68 results about "Brassica rapa" patented technology

The invention relates to the technical field of Brassica rapa polysaccharide detection, and discloses a quantitative detection method of Brassica rapa polysaccharide based on near infrared spectroscopy. The method comprises the following steps: acquiring near infrared spectrum data of a brassica rapa sample, and dividing detection stages by combining spectral characteristics to obtain a plurality of detection stage identifiers; performing differential mapping on the spectrum load spectrum according to the identifier to obtain an optical parameter set of each stage; constructing a staged near infrared spectrum quantitative model by using the set; performing importance weighting on the original spectral data according to the identifier to generate optimized spectral data; and extracting polysaccharide related characteristics from the optimized spectral data through a staged model, and outputting a predicted value of the content of the Brassica rapa polysaccharide. According to the method, through stage division, differential mapping and data weighting, the spectral data utilization rate and the model suitability are improved, a targeted technical path is provided for quantitative detection of Brassica rapa polysaccharide, and the method can be used for quality control of Brassica rapa related products.

The invention discloses a KASP molecular marker for identifying a Chinese cabbage nuclear male sterility gene and application of the KASP molecular marker. The invention belongs to the field of molecular biology, and particularly relates to a KASP molecular marker for identifying a Chinese cabbage nuclear male sterility gene and application of the KASP molecular marker. The method for identifying or assisting in identifying the Chinese cabbage fertility comprises the steps that the genotype of an SNP site in a Chinese cabbage genome to be detected is detected, the Chinese cabbage fertility is identified or assisting in identifying according to the genotype, the SNP site is an SNP site on a Chinese cabbage A07 chromosome, the nucleotide type of the SNP site is C or T, and the SNP site is the 1935th nucleotide of SEQ ID No: 4 in a sequence table. The SNP locus related marker can be used for Chinese cabbage male sterility molecular marker assisted breeding, and a new gene resource is provided for Chinese cabbage heterosis utilization.

The invention discloses a method for adjusting the pore opening degree of Shanghai green, and belongs to the technical field of plant detection. Shanghai leaves are placed in 20mL of open liquid containing (1) 0.5% potassium chloride and 100 [mu] M / L calcium chloride or (2) 0.5% potassium chloride and 50 [mu] M / L brassinolide or (3) 0.5% potassium chloride and 10 [mu] M / L cytokinin or (4) 0.5% potassium chloride and 5 [mu] M / L gibberellin or (5) 0.5% potassium chloride and 100 [mu] M / L methyl jasmonate, culture is performed under illumination, and the illumination time is set to be 5h, so that the pore opening degree of Shanghai is remarkably increased. Compared with a control group (containing 0.5% of potassium chloride), the pore openness of the treatment is respectively increased by 33.5%, 43.5%, 43.4%, 27.1% and 70.2%. The method disclosed by the invention lays a technical foundation for rapidly screening out the exogenous environmental stimulation factor (environical stimuli) capable of increasing the opening degree of the pore.

The application discloses a SNP molecular marker for identifying hypocotyl length of Brassica campestris L. var. and application thereof, and relates to the technical field of biotechnology.The molecular marker is SNP_LHY0701, which is located on a gene BraC07g017590 of the 7th chromosome of the Brassica campestris L.var., and a SNP site corresponds to a 119th base of a cDNA sequence of the gene BraC07g017590; when the base is C:C, the Brassica campestris L.var. shows long hypocotyls; and when the base is T:T, the Brassica campestris L.var. shows short hypocotyls.The KASP / PARMS technology is applied to gene typing of the SNP molecular marker, the hypocotyl length of the Brassica campestris L.var. can be quickly and accurately detected, the method has strong specificity, high-throughput and rapid detection can be achieved, the method can be simply and quickly applied to molecular-assisted breeding of the Brassica campestris L.var. suitable for mechanical harvesting, and the breeding efficiency is greatly improved.

The invention provides a gene for regulating and controlling thermo-sensitive male sterility characters of plants and application of the gene. Specifically, the thermo-sensitive male sterility gene is a newly identified gene, mutation of a gene coding region of the thermo-sensitive male sterility gene causes generation of thermo-sensitive male sterility characters, and a new material is provided for Chinese cabbage male sterility molecular mechanism research and breeding.

The invention discloses a DMP-based non-heading Chinese cabbage haploid creation method and application. According to the invention, gene editing is carried out on brassica rapa BrDMP, so that protein is lost or activity of brassica rapa BrDMP is reduced, and an obtained mutant plant is a non-heading Chinese cabbage haploid induction line; and carrying out selfing on the non-heading Chinese cabbage haploid induction line or the offspring of the non-heading Chinese cabbage haploid induction line, wherein the obtained selfing offspring is the non-heading Chinese cabbage haploid. The obtained haploid induction line of the non-heading Chinese cabbage can induce generation of haploid of the non-heading Chinese cabbage, and an effective way is provided for haploid breeding and double haploid creation of the non-heading Chinese cabbage.

The invention discloses an application of a BrSPDS1 gene in regulating and controlling the heat resistance of Chinese cabbage, the nucleotide sequence of the BrSPDS1 gene is shown as SEQ ID NO. 1, and the amino acid sequence of the BrSPDS1 gene is shown as SEQ ID NO. 2. The relationship between the BrSPDS1 gene and the heat resistance of the Chinese cabbage is identified by overexpressing and silencing the BrSPDS1 gene in the Chinese cabbage, and the heat resistance of the Chinese cabbage plant is enhanced by overexpressing the BrSPDS1 gene, namely the heat resistance of the Chinese cabbage is positively regulated by the BrSPDS1 gene. The BrSPDS1 gene silencing weakens the heat resistance of the Chinese cabbage, lays a foundation for further cultivation, screening or construction of a heat-resistant variety of the Chinese cabbage, and provides theoretical support for molecular breeding and improvement of the variety adaptability of such crops; new gene resources and theoretical basis are provided for Chinese cabbage heat-resistant breeding, and new application is provided for improving plant heat resistance by using the heat-resistant gene BrSPDS1.

The application provides a gene related to high-temperature resistance of Brassica campestris L. var. parachinensis Bailey and application thereof, relates to the technical field of genetic breeding, the gene is named as BrNAC043 gene, the nucleotide sequence of which is shown as SEQ ID NO. 1, and the amino acid sequence of which is shown as SEQ ID NO. 2, and the high-temperature resistance of each Brassica campestris L. var. parachinensis Bailey variety is judged according to the high-low expression of the BrNAC043 gene, wherein the high-temperature resistance of the Brassica campestris L. var. parachinensis Bailey variety with high BrNAC043 gene expression is better than that of the Brassica campestris L. var. parachinensis Bailey variety with low BrNAC043 gene expression, and the gene and method provided by the application are helpful to the genetic breeding of the Brassica campestris L. var. parachinensis Bailey.

The application relates to the field of cytology and relates to an oligonucleotide probe set design for identifying Brassica rapa chromosomes and application, which comprises the following steps: S1: obtaining a NR-TR set in a Brassica rapa R-o-18 reference genome; S2: statistically clustering the NR-TR set and obtaining TR array data of Brassica rapa chromosomes; S3: obtaining a repetitive sequence cluster which is enriched in all the chromosomes of Brassica rapa; S4: segmenting a repetitive sequence representative array of the obtained repetitive sequence cluster; and S5: respectively aligning the segmented sequences to the Brassica rapa R-o-18 reference genome by using blastn. The application establishes a technology for coating Brassica rapa chromosomes, and the technology can batch coat Brassica rapa chromosome materials.

The application provides a method for improving continuous cropping obstacle soil and improving quality of Chinese kale, comprising the following steps: selecting Chinese kale obstacle soil for five years, crushing the hardening layer, adjusting the soil pH, ploughing the soil to a depth of 30-40 cm, and layering the soil after 72 h; the soil is divided into two layers, 0-15 cm is the surface layer, and the composite microbial agent and organic-mineral complex are applied to the layer; 15-30 cm is the bottom layer, and the expanded perlite-polyacrylamide-ammonium polyphosphate composite particles are applied to the layer; installing a greenhouse and irrigation supporting facilities in the improved soil area; planting Chinese kale seedlings, irrigating micro-nano bubble water every two days, and removing the Chinese kale after 42 days. Through the synergistic effect of the composite microbial agent, organic-mineral complex and other substances, the soil physical and chemical properties are significantly improved, the growth index of Chinese kale is significantly improved, the SPAD value is increased, and the photosynthetic capacity is enhanced, which shows that the application has a significant promoting effect on the growth of Chinese kale and the repair of continuous cropping obstacle soil.

The application relates to the field of molecular breeding, in particular to a molecular marker for identifying interspecific hybrid of brassica rapa and coelonema and application thereof. The molecular marker comprises one pair of brassica rapa specific primers and one pair of coelonema specific primers. The identification method comprises the following steps: obtaining a sample to be identified, taking genomic DNA of the material as a template, and using two specific primer pairs for amplification; performing agarose gel electrophoresis operation on the amplified product; and judging the authenticity of the interspecific hybrid by observing the electrophoresis strip.

The invention relates to the technical field of molecular biology and plant breeding, and provides a black cabbage yellowing phenotype related KASP molecular marker, the marker corresponds to a specific SNP site of a black cabbage BcCHL27 gene, the SNP site is located at the 217bp position of the black cabbage BcCHL27 gene, and the basic group is mutated from A to G. The invention also provides a primer group for detecting the molecular marker. Meanwhile, the invention also provides application of the molecular marker or the primer group. Based on the KASP molecular marker, genotype identification and character screening of the yellow phenotype of the black cabbage are carried out, the accuracy, specificity and screening efficiency of yellow character detection of the black cabbage can be remarkably improved, rapid high-throughput identification of the yellow phenotype of the black cabbage in the seedling stage is achieved, an efficient tool is provided for genetic research of the yellow character of the black cabbage and molecular assisted breeding, and the application prospect is wide. Therefore, breeding cycle is shortened, resources are saved, and genetic modification of black cabbage is accelerated.

The application discloses a kind of phenylacrylamide derivatives and preparation method and purposes thereof, belong to organic synthesis chemistry and pesticide technical field.Phenylacrylamide derivatives are prepared by condensation reaction from cinnamic acid or its derivatives and benzimidazole derivatives.Biological activity test shows that the compounds exhibit different degrees of inhibitory activity on rice sheath blight pathogen, barley root rot pathogen, wheat scab pathogen and brassica rapa sclerotinia pathogen, and can be used for preparing fungicide for preventing and treating agricultural plant diseases.

The invention discloses an InDel molecular marker for detecting black spot resistance of broccoli, a method and application, and relates to the field of molecular biology. The site is located at 56724071bp-56724089bp of a broccoli chromosome 5, and the site has insertion / deletion variation of 18bp basic groups (the nucleotide sequence is as shown in SEQ ID NO. 1). When the site has a 18bp basic group (the sequence of an amplification product is shown as SEQ ID NO.2), the broccoli is a black spot resistant plant; otherwise, the plants are black spot sensitive plants. The invention also discloses a pair of specific primers for detecting the InDel molecular marker, and the nucleotide sequences of the specific primers are respectively shown as SEQ ID NO. 4 and SEQ ID NO. 5. The InDel molecular marker can rapidly and accurately detect the black spot resistance of broccoli germplasm resources at low cost, does not need field inoculation identification, can be used for early screening of broccoli black spot resistance varieties and molecular marker-assisted breeding, significantly improves the breeding efficiency, and shortens the breeding period.

The invention relates to the technical field of plant genetic engineering and molecular biology, and provides a BcRPGE1 gene for regulating petiole color differentiation of non-heading Chinese cabbage, and the nucleotide sequence of the BcRPGE1 gene is as shown in SEQ ID NO.1. The invention also provides an application of the gene in adjusting the petiole color differentiation of the non-heading Chinese cabbage and a specific method for adjusting the petiole color of the non-heading Chinese cabbage. In addition, the invention further provides application of the method in directional improvement of the petiole color of the non-heading Chinese cabbage and cultivation of non-heading Chinese cabbage varieties with different petiole colors. The BcRPGE1 gene has the advantages that the BcRPGE1 is determined as a key gene for regulating and controlling the petiole color differentiation of the non-heading Chinese cabbage for the first time, the directional regulation and control of the petiole color can be realized by regulating and controlling the gene, and a new gene resource and a technical support are provided for molecular regulation and control and breeding application of the petiole color of the non-heading Chinese cabbage.

The invention belongs to the technical field of gene engineering and crop breeding, and particularly relates to a brassica campestris ALS natural variant gene, a mutant protein and application thereof. The Brassica campestris ALS mutant gene BrALS3R is found in rapes for the first time, consists of 1959 bases, is naturally mutated and encodes rape acetolactate synthetase III (ALS3), and compared with a wild ALS3 gene, the 1074th base of the Brassica campestris ALS mutant gene BrALS3R mutates from C to A, so that the 358th amino acid of mutant protein encoded by the Brassica campestris ALS mutant gene BrALS3R mutates from aspartic acid to glutamic acid (D358E). A plant containing the BrALS3R gene has ALS herbicide resistance, so that the gene is introduced into a plant without ALS herbicide resistance by utilizing a crossbreeding or transgenosis method, and the resistance of a receptor plant to the ALS herbicide can be improved.

The present utility model relates to a feed formulation for ZAMPEN native chicken (Gallus gallus domesticus) hardening stage characterized by comprising of a basal diet formulation and supplemental feed formulation made from fermented forages. The basal diet formulation comprises: of 1 wt.% molasses, 0.1 wt.% vitamins and minerals, or a combination selected from the group comprising: energy-source component of 4.75 wt.% cassava, 7 wt.%, corn bran, 50 wt.% corn yellow; a crude protein component of 3.9 wt.% copra meal, 20 wt.% fish meal and 12 wt.% soybean meal; and a calcium source component of 0.35 wt.% eggshell, 0.9 wt.% limestone. Whereas the supplemental feed formulation comprises: tricanthera, azolla, duckweed, and vegetable trimmings of pechay (Brassica rapa), mustasa (Brassica juncea), alugbati (Basella alba), kangkong (Ipomoea aquatica), and camote tops (Ipomoea batatas). The said formulation aims to significantly enhance feed intake of the native chicken while reducing feed costs.

The invention discloses application of brassica rapa L. polysaccharide targeted Golgi apparatus stress in preparation of vascular endothelial cell protection drugs, and belongs to the field of traditional Chinese medicine application. Pharmacological studies show that the Brassica rapa L. polysaccharide can reduce the expression of Golgi apparatus related proteins GRASP65 and p-GRASP65 and the ratio of p-GRASP65 / GRASP65, and the expression level of cell related proteins is reduced along with the increase of the concentration of the Brassica rapa L. polysaccharide. A transmission electron microscope is adopted to observe the form of the Golgi apparatus, and it is further proved that the Brassica rapa L. polysaccharide has the effect of inhibiting the stress response of the Golgi apparatus. Mechanism research shows that the Brassica rapa L. polysaccharide can inhibit Golgi apparatus stress and improve endothelial cell functions by regulating RvE1 / Ras / ERK signal axes. The invention provides an experimental basis for application and development of the Brassica rapa L. polysaccharide in treatment of vascular diseases.

The invention discloses a DNA methylation regulatory gene identification method related to formation of cold resistance of Brassica campestris L. and application of the DNA methylation regulatory gene identification method. The method belongs to the technical field of winter rape cold resistance epigenetic regulation mechanism analysis. According to the invention, a methylation sensitive amplification polymorphism technology is utilized, four Chinese cabbage type winter rape materials with large cold resistance difference after the same variety is domesticated in different breeding environments are selected as research objects, the change rule of DNA methylation after treatment at 4 DEG C for 24 hours and recovery growth for 2 days is analyzed, and finally differential methylation gene expression is verified by utilizing a qPCR technology. The invention provides a reference method for DNA methylation research of plants under adversity stress, and compared with other epimics research such as DNA methylation, the method is simple and convenient to operate and low in cost, does not need high-throughput sequencing, and only needs one-generation sequencing. The candidate gene identified by the invention provides a genetic basis for explaining a cold resistance evolution molecular mechanism of Brassica campestris L. and guiding cold resistance improvement of northern winter rapes.

The present application relates to a kind of KASP molecular markers related to common pakchoi leaf purple and its application, by QTL-seq technology and genetic map location, the main effect QTL site related to leaf purple located on the 3rd chromosome of common pakchoi is found, and further developed the molecular marker closely linked to site, by the seed or seedling leaf extraction DNA of color unknown common pakchoi material, combined with the KASP primer of the present application carries out KASP molecular marker genotyping test, the color of common pakchoi can be predicted by the genotyping result of marker, without using artificial identification method, can obviously improve breeding efficiency, save breeding cost.

The disclosure provides, among other things, seeds and plants of Brassica rapa hybrids ‘E55B006’ and ‘E55B008’. The disclosure includes tissue cultures of Brassica rapa hybrids ‘E55B006’ and ‘E55B008’ and methods for producing a Brassica rapa plant that include crossing a Brassica rapa hybrid ‘E55B006’ plant or Brassica rapa hybrid ‘E55B008’ plant with itself or with another plant, such as a Brassica rapa plant of another genotype. The disclosure further includes seeds and plants produced by such crossing. The disclosure further includes plants, seeds, and tissue cultures of Brassica rapa hybrids ‘E55B006’ and ‘E55B008’ comprising introduced beneficial or desirable traits.

The invention discloses a probe method real-time fluorescent PCR (polymerase chain reaction) rapid detection primer and probe combination for cruciferae bacterial phytophthora blight and a detection method, and belongs to the technical field of biological detection. According to the probe method real-time fluorescent PCR rapid detection method for the cruciferae bacterial phytophthora blight, DNA of a tested strain is used as a template, and the probe method real-time fluorescent PCR rapid detection is carried out by using a primer and probe combination. The detection method has the advantages of high specificity, high accuracy, high sensitivity, rapidness, high efficiency, quantitative analysis, simple operation, good repeatability and strong applicability.

The invention relates to the technical field of biology, in particular to a Chinese cabbage hypocotyl length related molecular marker and application thereof. The invention firstly provides an application of the molecular marker related to the length of the hypocotyl of the Chinese cabbage or a substance for detecting the molecular marker related to the length of the hypocotyl of the Chinese cabbage in identification or auxiliary identification of the length of the hypocotyl of the Chinese cabbage. The molecular marker related to the length of the hypocotyl of the Chinese cabbage refers to a difference between sequence fragments of 49-420 sites of SEQ ID No.1 and sequence fragments of 49-155 sites of SEQ ID No.2. The molecular marker related to the length of the hypocotyl of the Chinese cabbage refers to the difference between the sequence fragments of 49-420 sites of SEQ ID No.1 and the sequence fragments of 49-155 sites of SEQ ID No.2. The molecular marker related to the length of the hypocotyl of the Chinese cabbage can be used for identifying or assisting in identifying the length of the hypocotyl of the Chinese cabbage in a seedling stage, and the breeding process of a new variety of the long-hypocotyl Chinese cabbage is accelerated.

The invention discloses an extraction method of Brassica rapa seed oil, and belongs to the technical field of compound extraction. According to the method, green solvent dimethyl ether (DME) is combined with a response surface method to optimize process parameters (pressure, temperature and time) for extracting the turnip seed oil, fatty acid composition of the obtained turnip seed oil is analyzed through gas chromatography, and saponified matter of the turnip seed oil is prepared and used for follow-up activity and functional evaluation. After optimization, under the conditions that the extraction pressure is 6 MPa, the extraction temperature is 47 DEG C and the extraction time is 83 min, the DME extraction rate (31.72%) of the method is higher than that (30.94%) of Soxhlet extraction of a traditional extraction method. More importantly, rare unsaturated fatty acids such as nervonic acid and the like are effectively retained by lower extraction temperature, and 21 fatty acids are identified by component analysis. The unique fatty acid composition guaranteed by an optimized process is directly associated with the remarkable biological activity of the fatty acid composition. Core advantages of the method in turnip seed oil extraction are proved, and scientific basis and technical support are provided for industrialization of turnip seed oil in the field of cosmetics.

The present invention discloses the use of the Brassica rapa BrPILS5 gene for improving plant pollen heat tolerance. The application involves inhibiting the expression of the Brassica rapa BrPILS5 gene to enhance pollen resistance to high temperature stress during pollen development. The inhibition of the Brassica rapa BrPILS5 gene co-suppresses the Brassica rapa BrPILS5a and BrPILS5b genes. Overexpression of an artificial miRNA fragment that inhibits the expression of the Brassica rapa BrPILS5 gene can improve heat tolerance in both the early and late stages of pollen development, reversing complete male sterility caused by high temperature conditions. This gene has promising application prospects for resistance breeding in Brassica rapa or other flowering plants.

The invention provides a brassica rapa probiotic fermented beverage as well as a preparation method and application thereof. The preparation method of the beverage comprises the following steps: 1) activating strains, and culturing lactobacillus rhamnosus and lactobacillus plantarum to obtain a seed solution; 2) mixing brassica rapa, jam and water in proportion, homogenizing and sterilizing to obtain a brassica rapa mixed solution; (3) adding the seed solution into the Brassica rapa mixed solution, uniformly mixing, and fermenting; and (4) filtering, compounding and sterilizing the fermented mixed solution to obtain a clear solution, namely the brassica rapa probiotic fermented beverage. The prepared Brassica rapa probiotic fermented beverage has high content of active ingredients such as polysaccharides, polyphenols and SOD, has good uric acid reduction and oxidation resistance, has a good health-care effect, has a good flavor, and reduces the bad flavor of Brassica rapa.

The invention provides an Mcic000159 gene of a CYP450 family gene participating in cantharidin biosynthesis, the nucleotide sequence of the Mcic000159 gene is as shown in SEQ ID NO. 1, and the coded amino acid sequence is as shown in SEQ ID NO. 2. When the expression of the Mcic000159 gene of the CYP450 family gene in the Eye spot Brassica rapa larva is interfered, the content of juvenile hormone III in the Eye spot Brassica rapa larva is not influenced, the content of cantharidin is reduced, and the relative expression quantity of the upstream genes McicHMGR, McicFPPS and McicJHEH for synthesizing the cantharidin is up-regulated. The Mcic000159 gene of the CYP450 family gene acts between the downstream metabolite of the juvenile hormone III and the cantharidin, so that the blank of the stage from the juvenile hormone III to the cantharidin in the synthetic route of the cantharidin is filled.

The invention discloses a molecular marker related to a root expansion character of a brassica plant and application of the molecular marker. The invention provides a DNA (Deoxyribose Nucleic Acid) molecule (Inde-BrrRSN1), which is shown as SEQ ID NO: 1. The invention also provides a primer pair which is composed of a primer F as shown in SEQ ID NO: 2 and a primer R as shown in SEQ ID NO: 3. According to the molecular marker and the primer pair provided by the invention, early identification and screening can be carried out on the fleshy root and non-fleshy root characters of the brassica plant through molecular identification before character phenotype observation, so that rapid breeding of the brassica plant is realized, and the molecular marker and the primer pair have application and popularization values.

The application relates to a CdRHB1 gene for reducing cadmium absorption and accumulation of plants and application thereof, and belongs to the technical field of biological genetic engineering. The CdRHB1 gene is a Brassica rapa BrCdRHB1 gene, and the nucleotide sequence is shown as SEQ ID NO. 1; or a radish RsCdRHB1 gene, and the nucleotide sequence is shown as SEQ ID NO. 2. The application refers to the following: knocking out the BrCdRHB1 gene can increase the cadmium absorption and accumulation of plants; or overexpressing the RsCdRHB1 gene can reduce the cadmium absorption and accumulation of plants. The application confirms for the first time through experiments that knocking out the BrCdRHB1 gene or overexpressing the RsCdRHB1 gene can increase or reduce the cadmium absorption and accumulation of plants, and lays a good theoretical and application foundation for cultivating low-cadmium and green crop varieties by using the CdRHB1 gene.