12 results about "Acetolactate Synthetase" patented technology

This invention belongs to the field of agricultural herbicides and provides a photosynthetic inhibitor herbicidal composition comprising 5-aminolevulinic acid, malondialdehyde, and a herbicidal active ingredient. The herbicidal active ingredient is selected from one or a combination of several herbicides selected from acetolactate synthase inhibitors, photosynthetic system II inhibitors, or protoporphyrinogen oxidase (PPO) inhibitors. This invention utilizes the synergistic effect of 5-aminolevulinic acid, malondialdehyde, and a specific type of herbicidal active ingredient to effectively enhance the efficacy of the herbicidal active ingredient, enabling rapid and thorough weed control.

The invention relates to the technical field of agricultural herbicides, and discloses a herbicide suitable for cnidium monnieri planting in southern districts and a preparation method thereof, the herbicide is composed of 4-8% by mass of nicosulfuron, 16-20% by mass of atrazine and 8-12% by mass of quizalofop-p-ethyl, and the balance is water. The mass ratio of nicosulfuron to atrazine is (1: 3)-(1: 5). The nicosulfuron and the atrazine have a dual mechanism of inhibiting the activity of acetolactate synthetase of weeds and blocking photosynthesis, the control effect within 30 days after application reaches 85% or above, gramineae weeds such as digitaria sanguinalis and barnyard grass and broadleaf weeds such as chenopodium album and amaranth can be effectively prevented and removed, only slight phytotoxicity such as transient leaf chlorosis is caused to cnidium monnieri plants, and the effect is remarkable. The quizalofop-p-ethyl inhibits the activity of weed acetyl-coenzyme A carboxylase and blocks fatty acid synthesis, and has a good control effect on green bristlegrass, eleusine indica and other grassy weeds.

The present invention provides a mutant ALS polypeptide and its application. Specifically, the present invention provides a mutant acetolactate synthase (ALS), wherein the mutant polypeptides include a mutant ALS1 and a mutant ALS3. The mutant ALS1 polypeptide is mutated at position 178 of SEQ ID No. 1 compared to the parent ALS1 polypeptide; and / or the mutant ALS3 polypeptide is mutated at position 172 of SEQ ID No. 2 compared to the parent ALS3 polypeptide. The mutated ALS polypeptide has strong tolerance to herbicides and has broad application prospects in the field of improving and cultivating plants resistant to ALS-inhibiting herbicides.

The invention provides a mutated ALS polypeptide and application thereof, and particularly provides a mutated acetolactate synthetase (ALS), the mutated polypeptide comprises a mutated ALS1 and a mutated ALS3, and compared with a parent ALS1 polypeptide, the mutated ALS1 polypeptide mutates at the 178th site corresponding to SEQ ID No.1, and the mutated ALS polypeptide mutates at the 178th site corresponding to SEQ ID No.2. And / or compared with the parent ALS3 polypeptide, the mutant ALS3 polypeptide is mutated at the 172nd site corresponding to the SEQ ID No.2. The mutated ALS polypeptide has very strong tolerance to herbicides, and has a very wide application prospect in the field of improving and cultivating plants resistant to ALS inhibitory herbicides.

This application provides herbicidal compositions containing (a) fluroxypyr or an agriculturally acceptable ester or salt thereof and (b) an acetolactate synthase (ALS) inhibitor herbicide, wherein the ALS inhibitor herbicide is diclosulam, chloransulam, chlorimuron, or tifensulfuron, or an agriculturally acceptable ester or salt thereof. The compositions provide synergistic weed control of undesirable vegetation in areas including, but not limited to, soybean, cotton, corn, sorghum, sunflower, sugarcane, sugar beet, alfalfa, cereals (including but not limited to wheat, barley, rice, and oats), uncultivated areas, fallow areas, perennial crop areas, fruit orchards, or plantation crops.

The invention relates to the technical field of agriculture, in particular to a compound herbicide for siberian cocklebur and a preparation method thereof, and the compound herbicide comprises 150 ml of benazolin, 60 ml of florasulam, 1.5 g of alkyl polyglycoside, 7 g of polyvinyl alcohol, 100 g of diatomite and 70 ml of distilled water. According to the compound herbicide, synergistic interaction of multiple action mechanisms is realized through scientific proportioning. The benazolin is used as a benzothiadiazinone herbicide and can inhibit an electron transport chain in plant photosynthesis; the florasulam is used as an acetolactate synthetase inhibitor and can block synthesis of branched chain amino acid. After the two components are compounded, the risk of drug resistance caused by long-term use of a single component is remarkably reduced. A composite herbicide is stirred through stirring blades, the composite herbicide is extruded through movement of the stirring blades, the extruded composite herbicide is crushed through crushing blades, and finally crushed composite herbicide particles are dried, so that the preparation process of the composite herbicide is simplified; the preparation efficiency of the compound herbicide is improved.

The invention relates to a weeding composition based on metamifop and cyhalofop-butyl and a preparation method thereof, and belongs to the technical field of pesticides, the weeding composition comprises the following raw materials by mass: 10-14 parts of an active component, 60-70 parts of a dispersion medium, 10-15 parts of an emulsifier, 3-5 parts of a dispersant, 1.5-2.5 parts of a thickener, 1-2 parts of a stabilizer, 3 parts of an anti-freezing agent and 0.5 part of an antifoaming agent; wherein the active ingredients comprise metazoloyl oxalic acid, cyhalofop-butyl and bispyribac-sodium, the dispersion medium comprises methyl oleate and vegetable oil, and the vegetable oil comprises one or a mixture of more of soybean oil, rapeseed oil, peanut oil and walnut oil; bispyribac-sodium is introduced as a third component to form a ternary compound system, the Bispyribac-sodium is used as a pyrimidine salicylic acid herbicide, and the action mechanism of the Bispyribac-sodium is that an acetolactate synthetase ALS inhibitor and a pyruvate synthetase ACCase inhibitor have no cross resistance.

The present invention relates to mixtures comprising: i) a first herbicide selected from the group consisting of the Very Long-Chain Fatty Acid inhibitors, preferably dimethenamid, dimethenamid-P or flufenacet; and ii) a second and a third herbicide each independently selected from the group consisting of the Very Long-Chain Fatty Acid inhibitors, preferably dimethenamid, dimethenamid-P or flufenacet, the Acetolactate Synthase inhibitors, preferably thiencarbazone, and the 4-Hydroxyphenylpyruvate Dioxygenase inhibitors, preferably mesotrione; provided that the first, second and third herbicides are different from each other. The invention further relates to formulations containing said mixtures and to a method for controlling weeds.

The application provides an engineered halomonas and a method for producing acetoin, wherein the halomonas expresses acetyl-lactate synthase and acetyl-lactate decarboxylase, and does not express PHA hydratase and acetoacetyl-CoA or the expressed PHA hydratase and acetoacetyl-CoA are not functional or have reduced function. The engineered halomonas can realize economic, sustainable and efficient production of acetoin, and improve single batch fermentation yield.

The present invention relates to a microorganism belonging to the genus Brevibacterium or Corynebacterium having improved L-isoleucine production capacity, the activity of acetolactate synthase inherent in the microorganism belonging to the genus Brevibacterium or Corynebacterium being weakened or inactivated, and a method for producing L-isoleucine using the microorganism belonging to the genus Brevibacterium or Corynebacterium. And meanwhile, the strain has the activity of foreign acetohydroxy acid synthetase II, so that compared with a parent strain, the strain has the advantages that less by-products are generated, and the production yield of the L-isoleucine can be improved.

The invention discloses a repelling and poisoning synergistic type artemisia apiacea-tolfenpxrad pharmaceutical composition and application thereof, the active ingredients of the pharmaceutical composition are composed of artemisia apiacea extract and tolfenpxrad, and the mass ratio of the artemisia apiacea extract to the tolfenpxrad is 1: 0.1-1: 1. The pharmaceutical composition obtained by compounding the artemisia apiacea extract and the tolfenpyrad can generate better repelling and poisoning effects on the red weevil and the rhinoceros rhinoceros, the prevention and treatment effects on the red weevil and the rhinoceros rhinoceros are improved, the pollution to the environment and pesticide residues can be reduced, and the pharmaceutical composition is worthy of popularization and application. Ecological prevention and control of palm plant pests such as red palm weevils and rhinoceros rhinoceros are facilitated, and the method is of great significance to prevention and control of palm plant pests.

The invention relates to a recombinant halomonas. The recombinant halomonas comprises an alsS (acetolactate synthase) gene, an ilvC (acetohydroxyacid isoreductase) gene, an ilvD (dihydroxy acid dehydratase) gene and an ilvE (transaminase) gene which are externally introduced. According to the recombinant halomonas disclosed by the invention, the L-valine can be efficiently produced without sterilization, in an energy-saving and water-saving manner through open fermentation, and the industrial production of the L-valine is facilitated.