Method for increasing purity of plant variety

A plant variety and purity technology, applied in the field of plant genetics and breeding, can solve problems such as direct economic losses, reduced grain production, and needs to be improved, so as to achieve the effect of ensuring purity and improving purity

Active Publication Date: 2012-11-07
BEIJING XINGBOYA BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, the production of false hybrid seeds affects the quality of the seeds due to the reduction of the purity of the hybrid seeds, resulting in direct economic losses;
Therefore, a method that can effectively eliminate "false hybrid seeds" and improve the purity of plant varieties is of great significance for improving food production, however, current methods still need to be improved

Method used

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  • Method for increasing purity of plant variety
  • Method for increasing purity of plant variety
  • Method for increasing purity of plant variety

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Genetic segregation analysis of rice imidazolinone herbicide-resistant mutants

[0038] First, make a Huanghuazhan rice mutant plant resistant to imazapyr herbicide (DNA sequence analysis shows that the acetolactate synthase (ALS) gene of this plant exists in a heterozygous state, and the ALS mutant gene is at 1642 and 1643 A mutation occurs at the site, from TG to AT, resulting in the 548th position of the corresponding encoded protein changing from tryptophan to methionine, that is, compared to the wild-type acetolactate synthase, its acetolactate synthase has Trp548Cys mutation) since Cross fruit in order to obtain selfed seeds. Then, the inbred seeds obtained above were subjected to seed soaking experiment and seedling spraying experiment respectively by using imazamazione water agent (purchased from Shandong Xianda Chemical Co., Ltd.), the specific steps are as follows:

[0039] 1. Seed soaking experiment

[0040] Using imazapyr water solution (0.12% b...

Embodiment 2

[0051] Embodiment 2: Utilize imidazolinone herbicides to carry out rice seed soaking experiment

[0052] The imidazolinone herbicides currently produced and sold in China include imazethapyr (imazapyr), imazethapyr (Bailongtong), imazethapyr (imazethapyr), and imazethapyr (imazaquin). Utilize the above-mentioned herbicide solution to soak the rice hybrid self-fertile seeds described in Example 1 for 24 hours, take out the seeds, and carry out germination in a 32°C incubator for 24 hours, and then germinate in a 25°C incubator, After 5 days, the germination and growth of small buds were observed.

[0053] The length of the small buds of various seeds was counted, and the results showed that the length of the small buds of the herbicide-resistant seeds reached 1.5-2 cm, and the length of the small buds of the non-herbicide-resistant seeds stopped at about 0.1-0.2 cm. Then, calculate the number of resistant individuals and non-resistant individuals in the seeds after each herbic...

Embodiment 3

[0057] Embodiment 3: Utilize imidazolinone herbicide to carry out rice seedling spraying experiment

[0058]Using imazapyr, bailongtong, imazethapyr and imazethapyr to carry out field seedling spraying experiments on the rice hybrid self-fertilizing seeds described in Example 1, specifically, the seedlings at the 2-leaf stage were sprayed. Spray 87 milliliters per square meter, 2-3 weeks after the spray treatment, count the seedling survival situation, the results are shown in Table 3 below. It can be seen from Table 3 that the segregation ratio of seedling resistance to various herbicides: non-resistance is close to 3:1, which is in line with the expected dominant inheritance. The results further indicate that non-resistant seedlings can be eliminated by herbicide spray treatment, thereby utilizing herbicide resistance characteristics to ensure the purity of crop hybrid seeds during field use.

[0059] Table 3 Four kinds of imidazolinone herbicides spraying two-leaf stage se...

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Abstract

The present invention relates to a method for increasing purity of a plant variety. The method comprises step of: providing a plurality of plant seeds, wherein at least a portion of the plant seeds express an acetolactate synthase mutant and have herbicide resistance; and cultivating the seeds to obtain plants with herbicide resistance. Before the seed cultivation or in the cultivation process, at least one of the following treatments is carried out: before cultivation, a herbicide is utilized to treat the seeds, in order to remove seeds without herbicide resistance; and during the cultivation, herbicide is utilized to treat seedlings, so as to remove the seedlings without herbicide resistance. The method can improve and guarantee purity of hybrid varieties in the field and endow the hybrid seeds and seedlings with herbicide resistance activity.

Description

technical field [0001] The invention relates to the field of plant genetics and breeding. Specifically, the present invention relates to a method for improving the purity of plant varieties, a method for preparing plant hybrid seeds and a method for improving and ensuring the purity of plant field hybrid varieties. Background technique [0002] Hybrid breeding is recognized as the most effective way to improve the overall productivity of crops. Hybrid breeding can cross different ecotypes and distantly related strains and varieties, and the hybrid seeds produced show characteristics beyond the parents, that is, heterosis. In recent decades, hybrid breeding has been widely used as a means to increase crop yield, improve crop quality, and improve crop resistance to insects, diseases, and stress, and has become the main breeding method for many crops. [0003] The key to producing high-purity hybrid seeds is to effectively control crop self-pollination and fertilization. At ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00A01H1/02A01G13/00
Inventor 马力耕唐晓艳王海洋邓兴旺
Owner BEIJING XINGBOYA BIOLOGICAL TECH
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