210 results about "Plant genetics" patented technology

The invention discloses a remediation method for heavy metal cadmium pollution of orchard soil based on galinsoga parviflora. The remediation method comprises the following steps: preparing galinsoga parviflora seedlings in a direct transplanting mode or a direct seeding mode; watering to guarantee the water-holding capacity of soil in a field and performing daily management on the galinsoga parviflora; performing harvesting treatment, i.e., harvesting the aboveground part of the galinsoga parviflora. The galinsoga parviflora has resistance and hyperaccumulation feature to cadmium heavy metal, the seedlings absorb and accumulate the cadmium heavy metal in the contaminated soil of the orchard, and the cadmium is transferred to the aboveground part to reduce the cadmium content in the orchard soil, so that the purpose of reducing the cadmium content in the contaminated soil of the orchard is achieved. In addition, the galinsoga parviflora has strong reproductive capacity, grows quickly and is shade-tolerant, the base part has strong branching capacity and can sprout after being harvested; the galinsoga parviflora can be planted almost all the year round in southern China and can grow in extremely barren land, the cost is low, the operability is high, and a new plant genetic resource is developed for phytoremediation of the heavy metal cadmium contaminated orchard soil.

The invention discloses a method for identifying hybrid germplasm of actinidia based on genome heterozygosity, relating to the technical fields of plant genetic resources and biology. The method comprises the following steps: (1) performing low-depth genome sequencing on a sample; (2) comparing reference genomes of sequencing data and mining single-base mutation sites; (3) calculating the heterozygosity of genome level of the sample based on the single-base mutation sites; (4) constructing a basic species heterozygosity distribution rule database of a main ancestor of the actinidia; and (5) performing heterozygosity comparison on a to-be-detected sample and the database so as to identify the hybrid germplasm. Compared with the traditional molecular marker technology, the method disclosed by the invention has the advantages that the identification accuracy is greatly improved; and meanwhile, compared with the traditional morphological identification method, the method disclosed by the invention is high-efficiency and rapid, and the identification time and cost can be saved. The method disclosed by the invention has wide applicability on different sequencing platforms, and comprehensive evaluation and application of the germplasm resources of actinidia can be formed.

The invention relates to the field of plant genetic breeding technology, and particularly discloses a method for creating a cymbidium resource for high-efficiency generation of 2n male gametes. The method includes parent matching, distant hybridization combination, hybrid colony construction, 2n male gamete identification, screening of the resource for high-efficiency generation of the 2n male gametes and the like. With utilization of the distant hybridization method, the cymbidium resource for high-efficiency generation of the 2n male gametes is obtained, and lays the foundation for high-efficiency creation of sexual polyploids by utilizing a sexual hybridization method, effective promotion of cymbidium polyploidy breeding, and cultivation of a breakthrough new cymbidium species in the absence of polyploids.