114 results about "Plant genetics" patented technology

The application discloses application of SbWOX gene and SbBBM gene in corn breeding and a corn genetic transformation method, and relates to a bioengineering technology.The application provides a nucleic acid molecule with co-expression of SbWOX protein gene and SbBBM protein gene, and corresponding gene co-expression vectors and microbial transformants.The application also provides application of the co-expression of the SbWOX gene and the SbBBM gene in the corn genetic transformation method.The application obtains corresponding genes and mutant proteins based on the SbWOX gene and the SbBBM gene of sorghum, the two genes are co-expressed in the corn genetic transformation process, even without adding plant growth hormones in the antibiotic screening medium in the tissue culture process, the plant genetic transformation process can be normally completed, and the transformation efficiency and the regeneration efficiency of the corn can be remarkably improved, the transformation rate reaches 10.42%, and the differentiation rate reaches 32.89%.

The present application relates to the technical field of plant genetic breeding, and particularly relates to a Brassica campestris fatty acyl reductase BcFAR2 gene, an expression protein thereof and application, a base sequence of the Brassica campestris fatty acyl reductase BcFAR2 gene is shown as SEQ ID NO. 1, and an amino acid sequence of the expression protein is shown as SEQ ID NO. 2, the Brassica campestris fatty acyl reductase BcFAR2 gene is cloned, and BcFAR2 gene overexpression Brassica campestris sterile plants can restore pollen activity; gene knockout Brassica campestris fertile plants can significantly reduce pollen activity; in Arabidopsis thaliana, overexpression of the gene can significantly improve salt tolerance of the transgenic plants; and in Brassica campestris, overexpression of the gene can significantly enhance salt tolerance.

The invention discloses a KASP molecular marker related to wheat stripe rust resistance characters and application of the KASP molecular marker, and belongs to the technical field of molecular markers and plant genetic breeding. SNP sites QYr.hzau4B of the KASP molecular marker are located at the 452, 424 and 208 basic groups of a wheat reference genome 4B chromosome, C / T mutation exists at the sites, and the nucleotide sequence of the KASP molecular marker is as shown in SEQ ID NO. 1. The KASP molecular marker can be used for identifying stripe rust resistant and susceptible varieties or strains of wheat, and a valuable molecular tool is provided for screening stripe rust resistant germplasm resources of wheat, so that the breeding efficiency of improved varieties is improved, and the breeding process is accelerated.

This invention discloses a genetic transformation and gene editing method applicable to multiple sesquiterpene species, belonging to the field of plant genetic transformation and gene editing technology. The method uses the cotyledonary nodes of sterile seedlings of the sesquiterpene genus as explants, and introduces an optimized pScEF1α-Cas9 gene editing transformation vector using Agrobacterium infection. Regenerated plants are obtained through recovery culture, selection culture, and rooting culture. The vector contains four gene expression units: pScEF1α:Cas9, pGmU6:sgRNA, pGmUBI:DsRed2, and pCaMV35S:HygR. In vivo screening is further performed using the DsRed2 fluorescent reporter gene, and the gene-edited plants are identified by molecular detection. This method is applicable to common sesquiterpene, stem-nodled sesquiterpene, and spiny sesquiterpene, and has advantages such as strong species versatility, high transformation efficiency, short cycle, visualized screening, and high positive rate, providing efficient technical support for gene function research and genetic improvement of sesquiterpene species.

The invention discloses a rice laodelphax striatellus resistance gene OsSAMS1 and application thereof. The gene provided by the invention is a DNA (Deoxyribonucleic Acid) molecule as shown in SEQ ID NO.2 and SEQ ID NO.3. The invention also provides a method for preparing the Corresponding proteins coded by the gene provided by the invention are proteins as shown in (a) and (b): (a) an amino acid sequence as shown in SEQ ID NO.1; and (b) a protein which is formed by substitution and / or deletion and / or addition of one or more amino acid residues of the amino acid sequence shown in SEQ ID NO.1, is related to rice laodelphax striatellus resistance and is derived from SEQ ID NO.1. The rice insect-resistant related protein provided by the invention affects the insect resistance of plants. Insect-sensitive plants can resist insects by improving expression of the protein coding gene, and laodelphax striatellus-resistant transgenic plants can be cultivated by silencing the gene. The gene and the encoding protein thereof can be applied to plant genetic improvement.

The present application relates to the field of plant genetic breeding, and particularly relates to a kind of evaluation method of asparagus yield. Including the following steps: S1, obtaining young plant period asparagus;S2, measures i variety of young plant period asparagus following parameters: photosynthetic rate A, ribulose-1,5-bisphosphate carboxylase / oxygenase activity B, leaf dry weight per unit area C, acid invertase activity D and root crown ratio E;The measurement sample of the acid invertase activity D is the tender stem of young plant period asparagus at 10-30cm from top;S3, according to the formula, the evaluation value is calculated, and the evaluation value is used to evaluate the yield of asparagus. The evaluation value obtained by using the above measurement index and calculation formula, and the significance level obtained by SPSS software is used to evaluate the yield of asparagus. The yield potential of asparagus can be comprehensively evaluated from the genetic point of view in a short time. Using the evaluation method of the present application, high-yield asparagus varieties can be selected in a short time without tracking for many years.

This invention relates to a genetic transformation method for Ningxia wolfberry using germinating seeds as explants, belonging to the field of plant genetic transformation technology. The invention mainly includes the following steps: (1) After disinfecting Ningxia wolfberry seeds, inoculate them onto a germination medium, and obtain germinating seeds through vernalization and dark culture treatment; (2) Activate and culture Agrobacterium rhizogenes and Agrobacterium tumefaciens strains carrying target gene expression frames to obtain a bacterial solution for infection; (3) Take the germinating seeds obtained in step (1) and mix them with the bacterial solution obtained in step (2) for infection, then transfer the infected germinating seeds to a co-culture medium or substrate for co-culture; (4) Cultivate the material after co-culture in step (3) to induce root growth, and detect the expression level of the target gene or reporter gene on the roots or plants to obtain transgenic positive plants. This invention has the advantages of simple operation, significantly shortened transformation cycle, and high transformation efficiency.

The invention provides an assembled and annotated tilia amurensis mitochondrial genome, and relates to the fields of molecular biology and plant genetics. The tilia amurensis mitochondrial genome is a single cyclic molecule; the total length of the tilia amurensis mitochondrial genome is 830088bp; the GC content of the tilia amurensis mitochondrial genome is 44.97%, the complete sequence of the tilia amurensis mitochondrial genome is reported for the first time, high repeatability and chloroplast sequence migration characteristics of the tilia amurensis mitochondrial genome are disclosed, and a molecular foundation is laid for endangered mechanism analysis, genetic diversity evaluation and protection strategy formulation of tilia amurensis.

The invention belongs to the technical field of plant biology, and particularly relates to a protein coded by a larch LarGRF1 gene and application of the protein to plant growth promotion. The invention aims to provide a new choice for plant genetic breeding by exploring growth regulatory factors (GRFs) of larch. The technical scheme of the invention is that the protein coded by the larch LarGRF1 gene has an amino acid sequence as shown in SEQ ID No. 2. Through overexpression of the MarGRF1 gene in dicotyledonous plants and monocotyledonous plants, it is found that an overexpression vector can promote proliferation of plant cells, especially proliferation of calluses and accelerate regeneration and growth.

The invention discloses an establishment method and application of an apple rootstock genetic transformation system for targeted knockout of an MdMLO gene, and belongs to the technical field of plant genetic transformation. The establishment method of the apple rootstock genetic transformation system comprises the following steps: cutting main leaf veins of tissue culture seedling leaves of apple rootstocks, and then putting the cut tissue culture seedling leaves into an infection solution to infect for 5-11 minutes; carrying out in-vitro regeneration culture on the infected tissue culture seedling leaves to obtain resistant seedlings; detecting MdMLO gene mutation sites of the resistant seedlings, and performing rooting culture on the resistant seedlings with mutated MdMLO genes to construct the apple rootstock genetic transformation system with stable powdery mildew resistance. According to the method, culture medium formulas in different stages are designed in the transformation process according to the characteristics of regeneration of 'Qingzhou No.1' apple rootstock leaves, and a Qingzhou genetic transformation system is established, so that effective delivery of a gene editing carrier is ensured.

The invention discloses application of a rice GL12 gene in regulation and control of rice grain shape, and belongs to the technical field of plant genetic engineering and plant genetic breeding. The invention provides the application of a rice GL12 gene related biological material for the first time, site-directed mutagenesis of the GL12 gene is carried out in a rice long-grain variety, and the grain length, the grain width and the thousand grain weight are all reduced. The GL12 gene is over-expressed in the rice short-grain variety, the grain length is increased, and the grain width and thousand grain weight have no stable difference. Therefore, the GL12 gene regulates and controls the rice grain shape character, and a theoretical basis is provided for rice grain shape research.

The invention provides a hormone inducible ZmEXPB6 promoter and application thereof in construction of a plant genetic transformation system, and belongs to the technical field of genetic engineering. The invention provides a hormone inducible ZmEXPB6 promoter. The nucleotide sequence of the hormone inducible ZmEXPB6 promoter is as shown in SEQ ID NO: 1. According to the embodiment of the invention, after a carrier containing the hormone-induced promoter and a target gene ZmWus2 gene is transferred into a corn immature embryo receptor, induced culture is carried out in a culture medium containing auxin 2 and 4-D. The result shows that the hormone-induced promoter drives the ZmWus2 gene to express, so that not only can a transgenic embryoid be quickly obtained and the conversion rate be increased, but also the yield of the corn immature embryo is increased. And compared with a constitutive promoter 35S promoter, the malformation rate of regenerated seedlings is obviously reduced. The hormone-induced promoter has a wide application prospect in the aspect of constructing a plant genetic transformation system.

The invention discloses rice OsARF6 and OsARF17 genes and application of the rice OsARF6 and OsARF17 genes in regulation and control of resistance of laodelphax striatellus. The protein provided by the invention is a protein as shown in (a) or (b): (a) a protein consisting of a sequence 1 in a sequence table and an amino acid sequence as shown in the sequence 1; and (b) a protein which is formed by substitution and / or deletion and / or addition of one or more amino acid residues of the sequence 1 and the amino acid sequence of the sequence 2, is related to plant insect resistance and is derived from the sequence 1 and the sequence 2. The plant insect-resistant related protein provided by the invention affects the insect resistance of plants. Insect-sensitive plants can resist insects by improving the expression of the protein coding gene, so that insect-resistant transgenic plants can be cultivated. The protein and the coding gene thereof can be applied to plant genetic improvement.

This utility model discloses an apparatus for plant genetic transformation culture, comprising a culture dish and culture bottle caps used in combination. The side of the culture dish is provided with graduations, including a minimum graduation for subculture, an intermediate graduation for differentiation culture, and a maximum graduation for rooting culture. The culture bottle caps include subculture bottle caps, differentiation culture bottle caps, and rooting culture bottle caps, each with ventilation holes at the top. This apparatus allows for precise control of the culture medium volume, avoiding waste and effectively reducing culture costs; it also improves the versatility of the apparatus, expanding its application range; and it facilitates observation and gas exchange, promoting healthy plant growth.

The present disclosure relates at least in part to genetic engineering of vegetable (e.g., tomato) plants to, for example, produce heme, a molecule contributing to a meat-like flavor, and to the incorporation of male sterility in these plants to facilitate the production of F1 hybrid seeds. Described herein, in some embodiments, are methods for constructing and introducing into such plants genetic constructs containing both a heme gene and a male sterility gene, the resulting plants, and the use of these plants in hybrid seed production. The present disclosure also provides at least in part methods for enhancing lycopene content in plants, such as tomato plants, either through genetic modification or selective breeding. While introducing key genes like Psy is effective, adding multiple genes or knocking out competing pathways can lead to more substantial increases in lycopene levels. Enhanced lycopene content can provide added health benefits, including potential cancer protection, making these plants, such as tomatoes, particularly valuable in the context of functional foods. The plants may be of varieties with high lycopene content combined with genetic modifications as provided herein. The present disclosure offers a comprehensive solution for producing plants (e.g., tomatoes) with increased nutritional value.

The application discloses a corn plant height regulation gene combination and application thereof in creating multi-gradient plant height corn materials, and the gene combination comprises a gene ZmCPS1, a gene ZmbHLH156 and a gene ZmEMF1L1. The application uses a homozygous near-isogenic line carrying a single regulation plant height gene in a corn 9782 background BC4 as a basic material, and then uses two-by-two hybridization of different gene near-isogenic lines, combines KASP molecular marker assisted screening based on a functional gene, gene chip detection, conventional breeding technology and plant height phenotype analysis, and finally, 8 three-gene aggregation corn materials with gradient plant height are obtained. The application aggregates different genotypes of alleles, and has high practical application value in plant genetics and breeding work.

The present invention relates to plant-based protein components suitable for use in consumables, including vegetarian or purely vegetarian meat or dairy analogues. The plant-based proteins have desirable organoleptic properties derived from improved methods in plant genetics.

The invention discloses application of a TaLAZY1 gene and a protein encoded by the TaLAZY1 gene in regulation and control of tillering angles of wheat, and belongs to the technical field of gene engineering. The technical problem to be solved by the invention is how to regulate and control the tillering angle of wheat. In order to solve the technical problem, the invention provides application of the TaLAZY1 protein or a substance for regulating and controlling the expression of a coding gene of the TaLAZY1 protein or a substance for regulating and controlling the activity or content of the TaLAZY1 protein in regulating and controlling the tillering angle of the plant. The TaLAZY1 protein can be a protein with an amino acid sequence as shown in SEQ ID NO. 3. According to the application, the coding gene of the TaLAZY1 protein is obtained through homologous cloning. The gene can remarkably regulate and control the tillering angle. The wheat gene TaLAZY1 can be widely applied to the plant fields of wheat genetic breeding, germplasm resource improvement, transgenosis, genome editing breeding and the like, and plays an important role in improving germplasm resources of crops such as wheat and the like.

The present application relates to the technical field of plant genetic breeding, and particularly relates to a wheat grain protein content related protein, a coding gene thereof and application.The wheat grain protein content related protein is a TaNPF5.5-2A protein and / or a TaNPF5.5-2B protein.The research shows that the grain protein content and the total nitrogen content of the TaNPF5.5-2B overexpression transgenic wheat strain have a significant advantage.The present application screens and mines a new wheat grain protein content related gene, which not only provides a theoretical clue for the molecular genetic research of the wheat grain protein content, but also provides important gene resources for the molecular breeding of the wheat quality.

The invention relates to the technical field of plant genetic transformation, and discloses a PEG-mediated rubber tree leaf protoplast large plasmid instantaneous transformation method which comprises the following steps: S1, taking 1-2g of leaves from the later stage of a bronze stage to a color change stage of a rubber tree within two years old, soaking the leaves in 0.6 M mannitol for 10 minutes, removing main leaf veins by using a scalpel, cutting the leaves into long and thin strips with the width of 0.5-1mm, transferring the long and thin strips into a 30ml enzyme solution, and carrying out enzyme deactivation on the long and thin strips; putting into a dark culture room at 28 DEG C, and carrying out enzymolysis treatment on a shaking table at 60 r / min for 5 hours; according to the method, transformation steps including centrifugal rotating speed, PEG action time and culture conditions are optimized, and a transformation reagent with a specific formula is adopted, so that the plasmid size limitation which is difficult to exceed in a rubber tree protoplast instantaneous transformation technology is broken through, large plasmids of more than 15kb are efficiently introduced into cells, and finally, the gene editing efficiency of more than 15% is achieved.

The invention discloses application of a soybean histone variant to improvement of genetic transformation efficiency of soybeans and other plants. By applying the soybean histone variant, the infection efficiency of agrobacterium to soybean cotyledonary node explants can be remarkably improved, so that efficient introduction of exogenous genes is promoted, the transformation efficiency is remarkably improved, finally, the higher stable transformation rate is obtained, and the application can be expanded to recalcitrant plant species with genetic transformation difficulty such as tea. In addition, the subsequent genetic transformation efficiency can also be remarkably improved by using the genetic transformation material for stable overexpression of the soybean histone variant as a receptor.

The invention discloses a molecular marker related to salt tolerance of peanuts and application of the molecular marker, and belongs to the technical field of molecular biology and plant genetic breeding. On the basis of screening the molecular marker related to the salt-tolerant character of the peanut, the molecular marker-assisted breeding method of the salt-tolerant variety of the peanut is established, and the new variety of the peanut with saline-alkali soil planting potential is cultivated. The molecular marker is an SNP (Single Nucleotide Polymorphism) site molecular marker linked with the salt tolerance character of the peanut, the SNP site molecular marker is located at the 77 site with the sequence of SEQ ID NO: 1, and the basic group of the SNP site molecular marker is G / A. The molecular marker related to the salt-tolerant character of the peanuts obtained through screening is used for screening salt-tolerant parents, so that the salt-tolerant genetic breeding speed of the peanuts is increased, and the breeding efficiency of salt-tolerant strains and the yield of saline-alkali soil are improved.

The application relates to a gene for controlling corn kernel size ZmILR1 and a molecular identification method and application thereof, and belongs to the technical field of plant genetic breeding and functional gene research. ZmILR1 The gene encodes an IAA-amino acid hydrolase, mutation of the gene leads to small corn kernels, and overexpression of the gene can create corn materials with increased kernel length, kernel width and hundred-kernel weight. According to the sequence difference between the mutant gene and the wild-type gene at the DNA level, a functional molecular marker A1A4 is developed, which can be used for rapid identification and screening of small-kernel corn materials. The application provides a theoretical basis and application tool for analyzing the molecular mechanism of corn kernel size, carrying out molecular marker-assisted breeding and hybrid selection, and provides important gene resources and technical support for corn high-yield and high-quality molecular breeding.

The invention relates to the technical field of plant genetic breeding, in particular to an efficient sorghum germplasm innovation method which aims at solving the problems that a traditional breeding period is long, gene editing application is limited and the like through genome evaluation and screening of candidate groups, optimization of a CRISPR / Cas9 system, construction of an efficient genetic transformation system, directional editing of polymerization characters, acceleration of breeding generations and special screening. And target varieties can be created in 3-4 generations. According to the method, the breeding period is shortened, the editing precision and the conversion efficiency are improved, multi-target characters can be aggregated, and the subdivision requirements of wine brewing, feeding and the like are met.

This invention belongs to the fields of genetic engineering and enzyme engineering, specifically relating to a sucrose synthase mutant, its preparation method, product, and application. The mutant is based on the amino acid sequence of the wild-type sucrose synthase shown in SEQ ID NO.1, with the 94th amino acid mutated from P to N, resulting in the amino acid sequence shown in SEQ ID NO.2; or the 660th amino acid mutated from Y to H, resulting in the amino acid sequence shown in SEQ ID NO.3. This mutant can be used for in vitro glycosylation reactions, plant genetic improvement, or industrial biocatalysis, solving the technical problems of poor stability and low catalytic efficiency of natural enzymes.

The invention belongs to the technical field of genetic engineering and plant genetic breeding, and particularly relates to a gene for regulating and controlling the cold tolerance of rice in the seedling stage and application of the gene. In order to excavate the gene for regulating and controlling the cold resistance of the rice, the nucleotide sequence of the rice gene OsCYP75B3 is mutated by virtue of a CRISPR / Cas9 genome targeted modification technology, and an experimental result shows that the cold resistance of the mutated rice in the seedling stage is remarkably reduced, so that the gene OsCYP75B3 is proved to negatively regulate and control the cold resistance of the rice in the seedling stage; the functional gene for regulating and controlling the cold resistance of the rice in the seedling stage is determined for the first time, and an important reference is provided for analyzing a molecular mechanism of the cold resistance of the rice in the seedling stage; based on the discovery, it is expected that the expression level of the gene OsCYP75B3 is improved through an overexpression technology, the gene OsCYP75B3 is applied to rice genetic engineering breeding, the cold resistance of rice is finally enhanced, and a key technical support is provided for guaranteeing grain safety.

The invention relates to the technical field of biological information processing, in particular to a cattle embryo pre-implantation genetic evaluation method and system based on whole genome sequencing, and the method comprises the following steps: obtaining whole genome sequencing data of a male parent and a female parent, embryo trophoblast live detection sequencing data and culture solution free desoxyribonucleic acid sequencing data; performing variation detection and haplotype phasing on male and female parent data to form haplotype data, and calculating a haplotype transmission posterior at an anchor point based on trophoblast data; performing genome segmentation based on reading depth observation and allele frequency observation of a trophoblast and a culture solution, and performing Bayesian inference by combining haplotype transfer posteriori as priori to obtain posteriori probabilities of aneuploid, copy number variation, chimera, pathogenic homozygosis and pollution events; and outputting a passing / rechecking / elimination conclusion, adding sequencing and updating the posteriori during rechecking until ending, and outputting a final conclusion and a genome breeding value, so that the evaluation reliability and the decision interpretability are improved.

The present application belongs to the technical field of plant genetic transformation, and particularly relates to a sorghum genetic transformation method based on inhibition of expression of sorghum tannin synthesis related genes. On the basis of the existing efficient regeneration genetic transformation platform using development regulators to promote cell regeneration, the present application introduces a precise molecular intervention strategy. In the early stage of sorghum genetic transformation, the expression of sorghum tannin synthesis related genes TAN1 gene, TAN2 gene and Y1 gene is inhibited, the two strategies of "enhancing intrinsic regeneration potential" and "eliminating external chemical inhibition" are organically integrated, and the dual optimization of "regeneration ability" and "transformation compatibility" is realized. Compared with the prior art, the present application can not only further significantly improve the transformation efficiency on the basis of the existing high level, but also improve the state of the recipient tissue, increase the survival rate of transgenic plants, and broaden the universality of the technical solution in different tannin content sorghum genotypes.

This invention discloses a tea plant liquid phase chip and its applications, belonging to the field of molecular detection technology. The invention discloses a tea plant liquid phase chip and its applications, which, based on site screening requirements and probe design principles, includes 5781 SNP sites. It can achieve genotyping of tea plant resources using precise localization sequencing and genotyping technology based on liquid phase capture of target genomic sequences. This tea plant liquid phase chip of the invention enables low-cost genotyping, primarily due to its tea plant specificity. It can facilitate tea plant variety identification and phylogenetic analysis, scientifically guide tea plant hybridization and improvement work, contribute to the protection and development of tea plant germplasm resources, and has high application value in multiple fields of tea plant breeding. The tea plant liquid phase chip of the invention can be used for tea plant genetic diversity assessment, germplasm resource and phylogenetic identification, genetic map construction and gene localization, genome-wide association analysis, and marker-assisted breeding of tea plants.

The invention discloses a rice nitrogen utilization related gene OsPHL-N and an encoded protein and application thereof, and the gene OsPHL-N provided by the invention is a DNA molecule as shown in 1), 2), 3) or 4): 1) a DNA molecule as shown in SEQ ID NO.1; 2) a DNA molecule as shown in SEQ ID NO. 2; 3) a DNA molecule which is hybridized with the DNA sequence limited by 1) or 2) under strict conditions and is used for coding the protein; and 4) a DNA molecule which has more than 90% of homology with the DNA sequence limited by 1), 2) or 3) and is used for coding the plant nitrogen utilization related protein. The invention also provides a protein coded by the gene, and the protein affects the utilization of plant nitrogen and can be applied to plant genetic improvement.