225 results about "Plant genetics" patented technology

The present invention relates to the fields of genetic engineering and plant genetic breeding, and specifically to the application of wheat TaPND gene. In the present invention, by taking gene TaPND as a target, designing a sgRNA sequence based on CRISPR / Cas9, connecting a DNA fragment containing the encoding sgRNA sequence to a vector carrying CRISPR / Cas, a plant binary expression vector capable of knocking out the TaPND gene in wheat is constructed; by genetically transforming wheat, offspring with TaPND gene knockout are obtained, and wheat mutants with early flowering time are obtained. Experiments have confirmed that transgenic wheat with TaPND gene knockout achieves the advancement of wheat flowering time. The technical scheme of the present invention provides a practical method for realizing rapid wheat breeding using genetic engineering technology, which has important breeding application value and broad market application prospects.

The invention belongs to the technical field of plant heredity and genetic engineering, and particularly relates to a wheat saline-alkaline tolerant protein kinase gene TaCRCK and application thereof. Specifically, the invention discloses a wheat saline-alkaline tolerant protein kinase gene TaCRCK, and the nucleotide sequence of cDNA of the gene is as shown in SEQ ID NO. 1. The invention also discloses a plant expression vector containing the gene TaCRCK and application of the gene and the plant expression vector in cultivation of saline-alkali resistant plants. Tests prove that silent expression of the gene promotes the resistance of wheat to saline-alkaline stress, and the saline-alkaline tolerance of transgenic plants is obviously improved. The gene has a negative regulation effect on the salt and alkali tolerance of plants, provides a new tool and method for improving the salt tolerance of crops by using a genetic engineering means, and has important scientific significance and application prospects.

The invention belongs to the technical field of plant heredity and genetic engineering, and particularly relates to a wheat saline-alkaline tolerant ERF transcription factor gene TaERF109 and application thereof. The invention discloses the wheat saline-alkali stress response gene TaERF109 obtained by cloning by utilizing the existing plant gene engineering technology for the first time, the gene is overexpressed in wheat through an agrobacterium tumefaciens mediated method, and comparative analysis proves that the saline-alkali tolerance of a transgenic plant is obviously improved, and the transgenic plant has a good application prospect. And interference on expression of the TaERF109 can reduce the resistance of the wheat to salt and alkali stress. Therefore, the invention fully proves that the wheat saline-alkaline tolerant ERF transcription factor gene plays an important role in plant saline-alkaline tolerant regulation and control, and shows that the gene can be widely applied to cultivation and improvement of plant saline-alkaline tolerant varieties, so that the wheat saline-alkaline tolerant ERF transcription factor gene has good practical application value.

The invention relates to the technical field of plant genetic breeding, in particular to a rice sheath blight resistance related gene and an encoding protein and application thereof. The invention provides a rice sheath blight resistance related gene as well as an encoding protein and application thereof, the gene is OsSBR4 or a related gene thereof, the resistance of rice to sheath blight can be remarkably enhanced by knocking out the gene, and the resistance of rice to sheath blight can be reduced by overexpressing the gene. After agronomic traits of a transgenic line of the gene are investigated, it is found that the plant height, the ear length, the ear number, the thousand seed weight and the like are not affected, and it is indicated that the gene has important application value in sheath blight resistance molecular breeding. According to the invention, a novel rice sheath blight resistance related gene is screened and excavated, and the identification of the sheath blight resistance gene can provide a theory and a basis for breeding a sheath blight resistance rice variety.

The invention discloses an efficient genetic transformation method of stem tumor sesbania, and belongs to the technical field of plant genetic transformation. According to the efficient genetic transformation method of the stem tumor sesbania, the cotyledonary node is used as the initial explant, callus induction is not needed, an optimized culture medium component is combined, a genetic transformation system of the stem tumor sesbania can be rapidly and effectively established, the genetic transformation efficiency can be improved and can reach 25% or above, the transformation time is shortened, and the cost is reduced. And a foundation is laid for domestication and accurate improvement work of the stem tumor sesbania.

The invention discloses a TaIMPA1 gene and application of the TaIMPA1 gene in improvement of plant genetic transformation efficiency. The nucleotide sequence of the gene is shown as SEQ ID No: 1 in a sequence table. According to the invention, the key gene TaIMPA1 is cloned from a plant body to delivery for the first time, and the genetic transformation performance is enhanced by remarkably improving the delivery efficiency of exogenous DNA (Deoxyribose Nucleic Acid). The discovery fills up the research blank of plant endogenous delivery regulatory factors, and an original solution is provided for overcoming the core problem of genotype dependence.

The invention relates to a gene ZmILR1 for controlling the size of a corn kernel as well as a molecular identification method and application of the gene ZmILR1, and belongs to the technical field of plant genetic breeding and functional gene research. The ZmILR1 gene encodes an IAA-amino acid hydrolase, mutation of the IAA-amino acid hydrolase causes corn kernels to become smaller, and overexpression of the gene can create a corn material with increased kernel length, kernel width and hundred-kernel weight. A functional molecular marker A1A4 is developed according to the sequence difference of the mutant gene and a wild type gene at the DNA level, and can be used for rapid identification and screening of small-grain corn materials. The invention provides a theoretical basis and an application tool for analyzing a molecular mechanism of the corn grain size and developing molecular marker-assisted breeding and hybrid seed breeding, and provides important gene resources and technical support for high-yield and high-quality molecular breeding of corn.

This invention discloses a rice starch synthesis-related gene, OsFLO17, its encoded protein, and its applications. Through phenotypic analysis of the rice endosperm mealy mutant flo17 and preliminary localization of the target gene, the starch synthesis-related protein OsFLO17 was ultimately cloned. This protein consists of the amino acid sequence shown in SEQ ID NO.3. The starch granule development-related protein of this invention affects the rice endosperm starch synthesis process. Introducing the encoding gene of this protein into plants with abnormal starch granules can yield transgenic plants with normal starch filling. Therefore, the protein and its encoded gene of this invention can be applied to plant genetic improvement.

The invention belongs to the technical field of plant genetic transformation, and particularly relates to a method for rapidly obtaining a citrus rootstock genetic transformation root system and application thereof. According to the genetic transformation method, agrobacterium rhizogenes K599 is used as a medium, an infection solution is transfected through a wound of a citrus plant, the infection solution can be quickly transfected into a stem segment of the citrus plant, and finally, the genetically transformed citrus plant can be quickly obtained in a high-pressure propagation mode. The method provided by the invention can effectively shorten the transformation system period, reduce the genetic transformation cost, improve the genetic transformation efficiency and avoid the generation of chimeras, and the materials can be repeatedly utilized.

The present invention provides a method for improving plant genetic transformation and gene editing efficiency. Specifically, the method improves the regeneration efficiency of plant genetic transformation and / or improves the efficiency of plant gene editing by expressing genes that promote plant cell division, especially meristematic cell division.

The invention discloses a tool for realizing efficient specific gene inhibition in a plant by utilizing a SunTag system of CRISPR-dCas9 (clustered regularly interspaced short palindromic repeats-das9). According to the method, gRNA near a transcription start site of a targeted target gene is designed, dCas9 and scFv-GFP are accurately recruited to a target site by using a SunTag system, the transcription start process of RNA polymerase II is interfered, and the expression of the target gene is remarkably inhibited. In view of relatively high activity of the system in a plant rdr6 mutant, an artificial miR-RDR6 fragment is further introduced into a vector and is used for inhibiting expression of RDR6 in a wild plant, so that the applicability and functionality of the system are expanded. Compared with a traditional method depending on a plant endogenous effector, the method has the advantages that dependence on endogenous regulatory factors is avoided, the specificity and safety of gene inhibition are remarkably improved, and the method can be used for plant genetic function research and character improvement.

The invention belongs to the technical field of plant heredity and genetic engineering, and particularly relates to a soybean alpha / beta folding hydrolase family gene GmSAL3 and application thereof. Specifically, an alpha / beta folded hydrolase family gene GmSAL3 is separated from soybean Williams 82, and tests prove that the gene GmSAL3 plays an important role in plant salt tolerance regulation and control. Specifically, GmSAL3 is expressed in hairy roots of arabidopsis thaliana and soybean, so that the root elongation and survival rate of transgenic arabidopsis thaliana seeds under salt stress are higher than those of contrast plants, and the salt tolerance of soybean hairy root transgenic plants is higher than that of contrast plants. Therefore, the gene can be widely applied to the improvement of the salt tolerance of plant varieties, and has good practical application value.

The invention discloses a plant gene editing system and method for delivering CRISPR (clustered regularly interspaced short palindromic repeats) / Cas9 based on mobile RNA (ribonucleic acid). The system does not depend on tissue regeneration or protoplast culture, Cas9 and gRNA are remotely delivered from the root to enter the overground part by fusing a CRISPR / Cas9 gene editing carrier of a tRNA-like sequence (TLS) movable motif and combining a hairy root transformation system, target gene editing is completed, and heritable gene editing seeds can be obtained at the current generation. The method comprises the following steps: 1, constructing a movable CRISPR / Cas9 gene editing fusion vector, wherein the movable CRISPR / Cas9 gene editing fusion vector comprises a target vector, an intermediate vector and an editing vector; and 2, application of the agrobacterium rhizogenes mediated plant hairy root transformation system. And 3, detecting the mobility and editing type of the movable editing carrier. And 4, gene function verification application. The system greatly breaks through the bottlenecks of difficult plant genetic transformation and long time consumption of precise breeding, and provides theoretical basis and technical support for gene function research and germplasm innovation of important agronomic traits of plants.

The application discloses application of SbWOX gene and SbBBM gene in corn breeding and a corn genetic transformation method, and relates to a bioengineering technology.The application provides a nucleic acid molecule with co-expression of SbWOX protein gene and SbBBM protein gene, and corresponding gene co-expression vectors and microbial transformants.The application also provides application of the co-expression of the SbWOX gene and the SbBBM gene in the corn genetic transformation method.The application obtains corresponding genes and mutant proteins based on the SbWOX gene and the SbBBM gene of sorghum, the two genes are co-expressed in the corn genetic transformation process, even without adding plant growth hormones in the antibiotic screening medium in the tissue culture process, the plant genetic transformation process can be normally completed, and the transformation efficiency and the regeneration efficiency of the corn can be remarkably improved, the transformation rate reaches 10.42%, and the differentiation rate reaches 32.89%.

This invention relates to the field of plant genetic transformation technology, and provides a method for genetic transformation of crape myrtle stem segments with buds. The method includes: infecting crape myrtle explants with Agrobacterium tumefaciens containing the RUBY reporter gene, and co-culturing them in a co-culture medium; then transferring the co-cultured explants to a differentiation medium for differentiation culture; and inoculating the obtained clustered buds into a rooting medium to obtain genetically transformed positive seedlings; wherein the explants are crape myrtle tissue culture seedlings with stem segments containing buds. The crape myrtle stem segments used in this invention have a higher regeneration efficiency than leaves. The adventitious bud induction rate of crape myrtle leaves is 1.0%-4.7%, and the stem segment regeneration and proliferation coefficient is 5-20. A large number of positive plants can be obtained in the later stage, and no browning phenomenon occurs in any of the stem segments after infection.

The present application relates to the technical field of plant genetic breeding, and particularly relates to a Brassica campestris fatty acyl reductase BcFAR2 gene, an expression protein thereof and application, a base sequence of the Brassica campestris fatty acyl reductase BcFAR2 gene is shown as SEQ ID NO. 1, and an amino acid sequence of the expression protein is shown as SEQ ID NO. 2, the Brassica campestris fatty acyl reductase BcFAR2 gene is cloned, and BcFAR2 gene overexpression Brassica campestris sterile plants can restore pollen activity; gene knockout Brassica campestris fertile plants can significantly reduce pollen activity; in Arabidopsis thaliana, overexpression of the gene can significantly improve salt tolerance of the transgenic plants; and in Brassica campestris, overexpression of the gene can significantly enhance salt tolerance.

This invention provides a gene encoding the E3 ubiquitin ligase BnaA02.SIP1 from Brassica napus and its application, belonging to the field of bioengineering technology. The nucleotide sequence of the BnaA02.SIP1 gene described in this invention is shown in SEQ ID NO:1. Specific primers were designed based on the coding region sequence of the BnaA02.SIP1 gene from Brassica napus, and a plant genetic transformation vector overexpressing the BnaA02.SIP1 gene was constructed using the constitutive expression promoter CaMV35S. The gene was then introduced into wild-type Arabidopsis thaliana and Brassica napus using Agrobacterium-mediated transformation, resulting in transgenic Arabidopsis thaliana and Brassica napus plants. The invention also verified that this gene plays a role in regulating salt tolerance in seedlings. This invention provides a theoretical basis and gene source for cultivating new salt-tolerant rapeseed varieties.

The invention discloses a KASP molecular marker related to wheat stripe rust resistance characters and application of the KASP molecular marker, and belongs to the technical field of molecular markers and plant genetic breeding. SNP sites QYr.hzau4B of the KASP molecular marker are located at the 452, 424 and 208 basic groups of a wheat reference genome 4B chromosome, C / T mutation exists at the sites, and the nucleotide sequence of the KASP molecular marker is as shown in SEQ ID NO. 1. The KASP molecular marker can be used for identifying stripe rust resistant and susceptible varieties or strains of wheat, and a valuable molecular tool is provided for screening stripe rust resistant germplasm resources of wheat, so that the breeding efficiency of improved varieties is improved, and the breeding process is accelerated.

This invention discloses a genetic transformation and gene editing method applicable to multiple sesquiterpene species, belonging to the field of plant genetic transformation and gene editing technology. The method uses the cotyledonary nodes of sterile seedlings of the sesquiterpene genus as explants, and introduces an optimized pScEF1α-Cas9 gene editing transformation vector using Agrobacterium infection. Regenerated plants are obtained through recovery culture, selection culture, and rooting culture. The vector contains four gene expression units: pScEF1α:Cas9, pGmU6:sgRNA, pGmUBI:DsRed2, and pCaMV35S:HygR. In vivo screening is further performed using the DsRed2 fluorescent reporter gene, and the gene-edited plants are identified by molecular detection. This method is applicable to common sesquiterpene, stem-nodled sesquiterpene, and spiny sesquiterpene, and has advantages such as strong species versatility, high transformation efficiency, short cycle, visualized screening, and high positive rate, providing efficient technical support for gene function research and genetic improvement of sesquiterpene species.

The invention provides a tilia amurensis primer combination, a DNA fingerprint spectrum, a variety identification method and application thereof, and relates to the field of molecular biology and plant genetics, the primer combination is a primer combination of an SSR molecular marker, and comprises a primer C110, a primer C840, a primer D150 and a primer TC5, and the primer sequences are shown as SEQ ID NO.1 to SEQ ID NO.8. The tilia amurensis primer combination is used for genetic diversity analysis of tilia amurensis, has the characteristic of high polymorphism, and can be used for identifying the variety of tilia amurensis. 70 polymorphic sites can be detected, different tilia amurensis individuals can be distinguished, the tilia amurensis DNA fingerprint spectrum can be constructed only through four pairs of primers, and the method is suitable for tilia amurensis germplasm resource protection, endangered population monitoring and molecular breeding.

The invention discloses a rice laodelphax striatellus resistance gene OsSAMS1 and application thereof. The gene provided by the invention is a DNA (Deoxyribonucleic Acid) molecule as shown in SEQ ID NO.2 and SEQ ID NO.3. The invention also provides a method for preparing the Corresponding proteins coded by the gene provided by the invention are proteins as shown in (a) and (b): (a) an amino acid sequence as shown in SEQ ID NO.1; and (b) a protein which is formed by substitution and / or deletion and / or addition of one or more amino acid residues of the amino acid sequence shown in SEQ ID NO.1, is related to rice laodelphax striatellus resistance and is derived from SEQ ID NO.1. The rice insect-resistant related protein provided by the invention affects the insect resistance of plants. Insect-sensitive plants can resist insects by improving expression of the protein coding gene, and laodelphax striatellus-resistant transgenic plants can be cultivated by silencing the gene. The gene and the encoding protein thereof can be applied to plant genetic improvement.

The present invention discloses a plant gluten sorting-related protein OsGPA15, its encoding gene and applications. Through phenotypic analysis of rice gluten sorting mutants gpa15 and preliminary mapping of target genes, the gluten sorting-related protein OsGPA15 was finally cloned. The related protein is composed of the amino acid sequence shown in SEQ ID NO.1, and the nucleotide sequence of the gene is as shown in SEQ ID NO.2 or SEQ ID NO.3. The gluten sorting-related protein of the present invention affects the sorting process of gluten in rice endosperm. By introducing the encoding gene of the protein into plants with reduced mature gluten content, transgenic plants with normal mature gluten content can be obtained. Therefore, the protein and its encoding gene of the present invention can be applied to plant genetic improvement.

The invention belongs to the field of gene engineering, and relates to a chalkiness-related protein OsGAPDH of rice endosperm as well as a coding gene and application of the chalkiness-related protein OsGAPDH. The protein provided by the invention is a protein as shown in (a) or (b): (a) a protein consisting of an amino acid sequence as shown in SEQ ID NO: 3 in a sequence table; and (b) a protein which is obtained by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence of SEQ ID NO: 3, is related to plant starch synthesis and is derived from SEQ ID NO: 3. The protein provided by the invention affects normal development of rice endosperm. The coding gene of the protein is introduced into Ningjing No.7 wild type, so that a transgenic plant of which the chalkiness of endosperm is obviously reduced can be cultivated. The protein and the coding gene thereof can be applied to plant genetic improvement.

The invention relates to the technical field of plant breeding, in particular to a mutant of rice ALS protein and application of the mutant. The mutant is obtained by mutating an amino acid sequence of a rice wild-type ALS protein as follows: serine at the 627th site is mutated into threonine, glycine at the 628th site is mutated into histidine, and amino acid at the 629th site is deleted. The application comprises the following steps: (1) enabling plants to have ALS inhibitor herbicide resistance; (2) the resistance of plants to ALS inhibitor herbicides is improved; (3) the phytotoxicity of ALS inhibitor herbicides to plants is reduced; (4) weed control; (5) plant genetic breeding or seed production; and (6) serving as a plant transgenic selection marker. The rice ALS protein mutant is obtained through resistance stress screening, the mutant is reported for the first time, rice can have high resistance to more than two ALS inhibitor herbicides, and the rice ALS protein mutant has high market value and social benefits.

The invention relates to the field of genetic engineering and plant genetic breeding, and discloses a soybean GmMYB6 protein and application of a coding gene thereof. The invention provides a GmMYB6 protein and a coding gene thereof, the GmMYB6 protein is closely related to the stalk strength and lignin content of a plant, and the stalk strength and lignin content of the plant can be effectively regulated and controlled by knocking out the coding gene of the GmMYB6 protein from the plant. The GmMYB6 protein and the coding gene thereof provided by the invention can be used for cultivating lodging-resistant soybean varieties, and have a wide application prospect.

The invention discloses a regulatory gene SmCytb5 of anthocyanin in eggplants and application of the regulatory gene SmCytb5, and relates to the technical field of biotechnology and plant genetic breeding. The SmCytb5 overexpression transgenic plant is constructed through experiments, compared with a wild type, the leaf color of the transgenic plant is changed from green to purple, the anthocyanin content is remarkably increased, and therefore it is proved that the SmCytb5 gene regulates expression of the anthocyanin in the eggplant, and a foundation is laid for a graduate to produce the eggplant rich in anthocyanin.

The present application relates to the field of plant genetic breeding, and particularly relates to a kind of evaluation method of asparagus yield. Including the following steps: S1, obtaining young plant period asparagus;S2, measures i variety of young plant period asparagus following parameters: photosynthetic rate A, ribulose-1,5-bisphosphate carboxylase / oxygenase activity B, leaf dry weight per unit area C, acid invertase activity D and root crown ratio E;The measurement sample of the acid invertase activity D is the tender stem of young plant period asparagus at 10-30cm from top;S3, according to the formula, the evaluation value is calculated, and the evaluation value is used to evaluate the yield of asparagus. The evaluation value obtained by using the above measurement index and calculation formula, and the significance level obtained by SPSS software is used to evaluate the yield of asparagus. The yield potential of asparagus can be comprehensively evaluated from the genetic point of view in a short time. Using the evaluation method of the present application, high-yield asparagus varieties can be selected in a short time without tracking for many years.

The invention belongs to the technical field of plant heredity and genetic engineering, and particularly relates to a cold-resistant and bolting-resistant related gene BraTZF3 of Chinese cabbage and application of the cold-resistant and bolting-resistant related gene BraTZF3. A stress-related transcription factor BraA01g008400.3 C is screened by combining a yeast stress library screening technology with transcriptome analysis, the stress-related transcription factor BraA01g008400.3 C belongs to a co-tandem C3H type zinc finger protein and comprises a C-x8-C-x5-C-x3-H zinc finger (ZNF) structural domain, and tests prove that the BraTZF3 is positively correlated to low-temperature stress response and cold resistance, and the BraA01g008400.3 C can be used for preparing the stress-related transcription factor BraA01g008400.3 C. According to the present invention, the BraTZF3 is subjected to heterologous overexpression to positively regulate the cold resistance and the bolting resistance of arabidopsis thaliana, such that the gene provides the positive regulation effect on the cold resistance and the bolting resistance of the plant, especially the Chinese cabbage, and the new tool and the new method are provided for improving the cold resistance and the bolting resistance of the crop by using the gene engineering means so as to provide the good practical application value;

The invention relates to the technical field of plant genetic improvement, in particular to application of a TaPLT8A gene or TaPLT8A protein to regulation and control of the iron content of monocotyledon seeds. The nucleotide sequence of the TaPLT8A gene disclosed by the invention is as shown in SEQ ID NO. 1; the amino acid sequence of the TaPLT8A protein is as shown in SEQ ID NO. 2. A result of a specific embodiment of the invention shows that the TaPLT8A gene is over-expressed in a wheat plant, so that the iron content of wheat grains can be remarkably increased; the gene is knocked out of wheat, so that the iron content of wheat grains is remarkably reduced. Therefore, the TaPLT8A gene or the TaPLT8A protein can regulate and control the iron content in the wheat grains, and the research provides an available gene resource for cultivating high-iron wheat varieties.

This invention relates to a genetic transformation method for Ningxia wolfberry using germinating seeds as explants, belonging to the field of plant genetic transformation technology. The invention mainly includes the following steps: (1) After disinfecting Ningxia wolfberry seeds, inoculate them onto a germination medium, and obtain germinating seeds through vernalization and dark culture treatment; (2) Activate and culture Agrobacterium rhizogenes and Agrobacterium tumefaciens strains carrying target gene expression frames to obtain a bacterial solution for infection; (3) Take the germinating seeds obtained in step (1) and mix them with the bacterial solution obtained in step (2) for infection, then transfer the infected germinating seeds to a co-culture medium or substrate for co-culture; (4) Cultivate the material after co-culture in step (3) to induce root growth, and detect the expression level of the target gene or reporter gene on the roots or plants to obtain transgenic positive plants. This invention has the advantages of simple operation, significantly shortened transformation cycle, and high transformation efficiency.