202 results about "Plant genetics" patented technology

The present invention is in the field of plant genetics and provides recombinant nucleic acid molecules, constructs, and other agents associated with the coordinate manipulation of multiple genes in the fatty acid synthesis pathway. In particular, the agents of the present invention are associated with the simultaneous enhanced expression of certain genes in the fatty acid synthesis pathway and suppressed expression of certain other genes in the same pathway. Also provided are plants incorporating such agents, and in particular plants incorporating such constructs where the plants exhibit altered seed oil compositions.

The present invention is in the field of plant genetics. More specifically the invention relates to nucleic acid molecules and nucleic acid molecules that contain markers, in particular, single nucleotide polymorphism (SNP) and repetitive element markers. In addition, the present invention provides nucleic acid molecules having regulatory elements or encoding proteins or fragments thereof. The invention also relates to proteins and fragments of proteins so encoded and antibodies capable of binding the proteins. The invention also relates to methods of using the nucleic acid molecules, markers, repetitive elements and fragments of repetitive elements, regulatory elements, proteins and fragments of proteins.

The present invention is in the field of plant genetics and provides recombinant nucleic acid molecules, constructs, and other agents associated with the coordinate manipulation of multiple genes in the fatty acid synthesis pathway. In particular, the agents of the present invention are associated with the simultaneous enhanced expression of certain genes in the fatty acid synthesis pathway and suppressed expression of certain other genes in the same pathway. Also provided are plants incorporating such agents, and in particular plants incorporating such constructs where the plants exhibit altered seed oil compositions.

The present invention is in the field of plant genetics and provides recombinant nucleic acid molecules, constructs, and other agents associated with the coordinate manipulation of multiple genes in the fatty acid synthesis pathway. In particular, the agents of the present invention are associated with the simultaneous enhanced expression of certain genes in the fatty acid synthesis pathway and suppressed expression of certain other genes in the same pathway. Also provided are plants incorporating such agents, and in particular plants incorporating such constructs where the plants exhibit altered seed oil compositions.

The present invention is in the field of plant genetics and biochemistry. More specifically, the present invention relates to genes and polypeptides associated with the tocopherol biosynthesis pathway, namely those encoding 4-Hydroxyphenylpyruvate Dioxygenase activity, and uses thereof.

The invention discloses a remediation method for heavy metal cadmium pollution of orchard soil based on galinsoga parviflora. The remediation method comprises the following steps: preparing galinsoga parviflora seedlings in a direct transplanting mode or a direct seeding mode; watering to guarantee the water-holding capacity of soil in a field and performing daily management on the galinsoga parviflora; performing harvesting treatment, i.e., harvesting the aboveground part of the galinsoga parviflora. The galinsoga parviflora has resistance and hyperaccumulation feature to cadmium heavy metal, the seedlings absorb and accumulate the cadmium heavy metal in the contaminated soil of the orchard, and the cadmium is transferred to the aboveground part to reduce the cadmium content in the orchard soil, so that the purpose of reducing the cadmium content in the contaminated soil of the orchard is achieved. In addition, the galinsoga parviflora has strong reproductive capacity, grows quickly and is shade-tolerant, the base part has strong branching capacity and can sprout after being harvested; the galinsoga parviflora can be planted almost all the year round in southern China and can grow in extremely barren land, the cost is low, the operability is high, and a new plant genetic resource is developed for phytoremediation of the heavy metal cadmium contaminated orchard soil.

The present invention is in the field of plant genetics. More specifically the invention relates to nucleic acid molecules and nucleic acid molecules that contain markers, in particular, single nucleotide polymorphism (SNP) and repetitive element markers. In addition, the present invention provides nucleic acid molecules having regulatory elements or encoding proteins or fragments thereof. The invention also relates to proteins and fragments of proteins so encoded and antibodies capable of binding the proteins. The invention also relates to methods of using the nucleic acid molecules, markers, repetitive elements and fragments of repetitive elements, regulatory elements, proteins and fragments of proteins.

The invention belongs to the technical field of plant genetic engineering and relates to the isolation, identification and use of a plant stress specifically inducible promoter Oshox24P. A rice drought-inducible promoter Oshox24P, which has a nucleotide sequence SEQ ID No.1 in a sequence table, is obtained by isolation and identification. The promoter of the invention can be used in plant genetic engineering for plant genetic improvement, in particular for plant drought resistance improvement.

The present invention is in the field of plant genetics. More specifically the invention relates to nucleic acid molecules and nucleic acid molecules that contain markers, in particular, single nucleotide polymorphism (SNP) and repetitive element markers. In addition, the present invention provides nucleic acid molecules having regulatory elements or encoding proteins or fragments thereof. The invention also relates to proteins and fragments of proteins so encoded and antibodies capable of binding the proteins. The invention also relates to methods of using the nucleic acid molecules, markers, repetitive elements and fragments of repetitive elements, regulatory elements, proteins and fragments of proteins.

The invention belongs to the technical field of plant biology and particularly relates to separation, function identification and application of a rice anther specific expression promoter. The disclosed promoter is specifically expressed in a plant anther and has good application prospects in the field of plant genetic modification.

The invention discloses a method for identifying hybrid germplasm of actinidia based on genome heterozygosity, relating to the technical fields of plant genetic resources and biology. The method comprises the following steps: (1) performing low-depth genome sequencing on a sample; (2) comparing reference genomes of sequencing data and mining single-base mutation sites; (3) calculating the heterozygosity of genome level of the sample based on the single-base mutation sites; (4) constructing a basic species heterozygosity distribution rule database of a main ancestor of the actinidia; and (5) performing heterozygosity comparison on a to-be-detected sample and the database so as to identify the hybrid germplasm. Compared with the traditional molecular marker technology, the method disclosed by the invention has the advantages that the identification accuracy is greatly improved; and meanwhile, compared with the traditional morphological identification method, the method disclosed by the invention is high-efficiency and rapid, and the identification time and cost can be saved. The method disclosed by the invention has wide applicability on different sequencing platforms, and comprehensive evaluation and application of the germplasm resources of actinidia can be formed.

The invention provides a transformation method utilizing a red fluorescent protein as a selection marker. The method comprises the following steps of: synthesizing a fluorescent protein (FP) gene and modifying according to the bias of a rice codon, wherein the modified FP gene is used as a selective marker gene of rice transformation and plant regeneration; driving the FP gene by a callus/seed coat-specific promoter, closely connecting with a target gene, and transferring to rice embryonic calli under the mediation of Agrobacterium tumefaciens; screening for three times by a fluorescence microscope in 30 days after coculture; and further verifying an obtained transgenic seedling by utilizing a polymerase chain reaction (PCR) and Southern hybridization. The result shows that the positive rate of the transgenic seedling is up to 80 percent, which proves that FP serving as the selection marker of plant transformation is completely feasible. By the method, the potential hazard of the traditional selection markers such as antibiotic and herbicide resistance genes and the like to environment and food safety is effectively reduced. The invention provides a novel safe plant genetic transformation screening system.

The invention relates to the field of plant genetic breeding technology, and particularly discloses a method for creating a cymbidium resource for high-efficiency generation of 2n male gametes. The method includes parent matching, distant hybridization combination, hybrid colony construction, 2n male gamete identification, screening of the resource for high-efficiency generation of the 2n male gametes and the like. With utilization of the distant hybridization method, the cymbidium resource for high-efficiency generation of the 2n male gametes is obtained, and lays the foundation for high-efficiency creation of sexual polyploids by utilizing a sexual hybridization method, effective promotion of cymbidium polyploidy breeding, and cultivation of a breakthrough new cymbidium species in the absence of polyploids.

The invention relates to the field of gene engineering, in particular to a multi-gene binary expression vector constructed by using homologous recombination. The multi-gene binary expression vector is formed by connecting single genes or multiple genes into a multi-gene polymer through polyclonal enzyme cutting sites so as to form a homologous recombination intermediate vector and adding the single-gene or multi-gene polymer into a basic binary vector by homologous recombination, wherein the homologous recombination can be performed for multiple times, so that the T-DNA length of the basic binary vector is gradually increased. The invention also discloses a construction method of the multi-gene binary expression vector and application in plant transgenes. The multi-gene binary expression vector applied in plant genetic modification can transfer multiple target genes at the same time, and has the advantage of high co-transformation efficiency.

The invention discloses a composition of plant direct gene transformation, transforming method and appliance, which is characterized by the following: making the component include goal gene and free nucleic acid component of adjusted and controlled element, transforming buffer liquid and transforming assistance component; co-culturing; soaking; surface-daubing; injecting; dripping; spraying; transforming with dip flower method or through genetic gun bombardment, supersonic wave and electric shock method. This component can be used to receptor plant cell direct transformation of fertilized ovum, suspended cell, protoplast, single-layer cell and diachyma cell and can be used to seed, receptor plant pollen, pistil, flower pillar, pollen pipe and ovary, which possesses impotent meaning for plant genetic seeding, variety improvement and gene functional analysis.

The present invention discloses one kind of antibacterial peptide gene of Chinese prawn containing single whey acidic protein structure domain and its coded antibacterial peptide and application in preparing antibacterial active recombinant protein. The recombinant antibacterial peptide gene of Chinese prawn containing single whey acidic protein structure domain of the present invention may be applied in antibacterial feed additive, food preservation, animal and plant genetic conversion and medicine development.

The invention relates to a thermostatic male-killing device for rice seed production, belonging to the technical field of plant genetic breeding technology. The thermostatic male-killing device for rice seed production is characterized in that a container body (1) is located in an outer shell (2), a middle electric heating device (3) is arranged at the middle part inside the container body (1), and a bottom electric heating device (4) is arranged at the bottom; a bottle cap (6) is arranged at the bottleneck which is at the upper part of the container body (1); a handle (5) is arranged at the outer side of the outer shell (2); a thermostatic control device (8) is arranged at the upper part of the outer side of the outer shell (2), and an externally connected power socket (7) is arranged at the lower part of the outer side of the outer shell (2); the middle electric heating device (3), the bottom electric heating device (4) and the thermostatic control device (8) are connected with the externally connected power socket (7) through wires and are connected with an outer power source. The thermostatic male-killing device controls temperature in the male-killing device automatically through a thermostatic controller, and the temperature after being set is kept constant basically, so that the high-temperature male-killing effect is guaranteed, the male-killing accuracy is improved, the labor intensity is reduced, and the work efficiency is increased.

The invention discloses in-vitro gene stacking technology compatible with recombinase-mediated in-vivo gene stacking and application of in-vitro gene stacking technology. The in-vitro gene stacking technology includes element forming of in-vitro gene stacking series vector and an in-vitro gene stacking method. The in-vitro gene stacking method includes: utilizing an irreversible site-specific recombination system for cointegration between two DNA molecules; utilizing a reversible site-specific recombination system to delete unneeded DNA sequences; utilizing a second irreversible site-specific recombination system to transfer multigene vectors which are stacked well in vitro into a binary vector suitable for agrobacterium transformation for plant genetic transformation, or directly integrating the multigene vectors on a specific seat of a genome in a targeted manner through site-specific recombination in vivo. The in-vitro gene stacking technology meets needs of in-vitro gene stacking and is compatible with an in-vivo recombinase-mediated gene stacking system.

The invention discloses a purification method of plant endogenous brassinolide based on boron affinity solid phase extraction. According to the method, the plant endogenous brassinolide is extracted by a solvent, an extract liquid directly acts on a boron affinity material, the plant endogenous brassinolide is adsorbed on the surface of the material, matrix impurities are removed through leaching,and then the brassinolide adsorbed on the boron affinity material is eluted through an oxidation-hydrolysis-reverse phase elution strategy. The method is easy to operate, good in selectivity, high inrecovery rate, and thorough in removal of the matrix interference, can be used for high-sensitivity and quantitative analysis of trace brassinolide in plant materials, and has wide application prospects in plant genetics, crop science and other fields.

The invention relates to a method for creating male sterile lines in plant by genetic engineering. According to the structural and functional characteristics of the Ssp DnaE intein internal peptide and Barnase toxoprotein, the method is used to construct a self propagation line A and line B; and the hybrid filial generation shows sterility through the hybridization between the line A and the line B. After the hybridization of the sterile line and any other non-transgene fertile line, the fertility of the hybrid filial generation is restored. The method solves the problem that the fertility of a sterile line obtained by a conventional genetic engineering method cannot be restored by a simple method.

The invention relates to the field of plant genetics, in particular to a cotton fiber length-related quantitative trait locus (QTL) and application thereof. The cotton fiber length-related QTL qUHM-1-1 is positioned at chromosome 1, and the side adjacent marker of the cotton fiber length-related QTL qUHM-1-1 is BNL3580-BNL1667; the cotton fiber length-related QTL qUHM-22-1 is positioned at chromosome 22, and the side adjacent marker of the cotton fiber length-related QTL qUHM-22-1 is NAU3093-CICR345. The cotton fiber length-related QTL can be detected in two environments, and has stability which does not depend on the environment.