282 results about "Plant biology" patented technology

The invention relates to a rice salt-tolerant gene OsRR22 mutant, an encoded amino acid sequence thereof, a plant and a making method of the mutant, and belongs to the technical field of plant biology. A CRISPR/Cas9 technology is sued to edit self-selected rice variety WDR58 with rice salt-tolerant gene OsRR22 to obtain a rice salt-tolerant gene OsRR22 function deleted mutant new germplasm WDR58-cas-1 with important application values. The mutant can significantly increase the salt tolerance of rice, and can be applied to high-yielding salt-tolerant rice breeding.

The invention belongs to the technical field of plant biology, and relates to a transgenic soybean event B4J8049 exogenous inserted fragment flanking sequence and applications thereof. The provided anti-disease transgenic soybean event B4J8049 is identified as unit point insertion through the Southern hybrid, and the right boundary flanking sequence of the exogenous fragment of the insertion site is represented by SEQ-1. The specific primers, which are designed according to the flanking sequence, are represented by the SEQ-5 and SEQ-6, and a specific PCR detection method of the anti-disease transgenic soybean event B4J8049 is established. The provided detection primer and detection method are suitable for carrying out specific detection on the anti-disease transgenic soybean event B4J8049, which comprises parent, derivation species, and products (plants, tissue, seed, and product).

The invention provides a molecular marker for a paddy recessive genic male sterility gene cyp704b2 and application thereof, belonging to the technical field of plant biology. The molecular marker provided by the invention comprises two forward primers SEQ ID NO.1 and SEQ ID NO.2 and one reverse primer SEQ ID NO.3. The molecular marker can be utilized to complete the genetic typing of the paddy recessive genic male sterility gene cyp704b2 on the basis of conventional PCR and PAGE glue electrophoresis. The molecular marker has the advantages of simplicity and convenience in operation, high typing speed, accurate result and low cost, is capable of increasing the selection efficiency of the target trait and can meet the requirements of large-scale molecular marker-assisted selection breeding.

The invention belongs to the technical field of plant biology and particularly relates to separation, function identification and application of a rice anther specific expression promoter. The disclosed promoter is specifically expressed in a plant anther and has good application prospects in the field of plant genetic modification.

The invention belongs to the technical field of plant biology, and particularly discloses a molecular marker for a rice aroma gene and application thereof. The molecular marker consists of a pair of outer primers Badh2-O-F and Badh2-O-R and a pair of inner primers Badh2-WT-F and Badh2-E7-R, wherein the primer sequences are shown as SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3 and SEQ ID NO:4; an amplified fragment of the marker is moderate and is high in specificity; the rice gelatinization temperature control gene badh2-E7 can be genotyped into aroma rice and aroma-free rice varieties; auxiliary selection of the molecular marker of the rice aroma characteristics can be realized by utilizing the marker; the breeding efficiency is improved; the requirement on large-scale molecular breeding is met.

The invention belongs to the technical field of plant biology, in particular relates to a method of tissue culture of Medicago sativa L. By way of developing a novel tissue culture technical method, the invention focuses on improvement and innovation of two technical nodes (seed disinfection and callus formation and differentiation) in culture steps. With adoption of a mercury bichloride and hydrogen peroxide compound disinfection method, the invention realizes the purposes of thorough disinfection effect and low pollution rate and minimum damage to seeds. Finally, the invention solves the problem of severe pollution to current explants. Meanwhile, a method of TDZ and 6-BA is adopted, thereby, the callus induction rate and the formation rate are remarkably increased, the callus quality is improved, the differentiation capacity of the callus is reserved to a maximum extent, the formation of embryoid sprouts is promoted so that the novel technical method of the tissue culture of the Medicago sativa L. which is efficient, saves time, and has low toxicity and low browning, is obtained.

The present invention relates to the fields of molecular biology and plant biology. Specifically, the invention is directed to the methods for expressing spider silk proteins in plants and the synthesis and purification of spider silk proteins therefrom.

The invention belongs to the technical field of plant biology and in particular relates to a method for extracting paeonol from paeonia suffruticosa roots. The method comprises the steps of picking, complex enzyme liquid preparation, ultrasonic treatment, centrifugal treatment and reduced pressure distillation. The method has the beneficial effects that enzyme treatment is firstly carried out on paeonia suffruticosa roots and barks at 30 DEG C for 20 minutes, so that the cell walls of the paeonia suffruticosa roots and barks and the interstitial structures of the cell walls can suffer from changes such as local loosening, swelling, collapse and the like; short-time ultrasonic treatment is then carried out on the paeonia suffruticosa roots and barks, ultrasonic waves generate strong impact waves and microjet inside the liquid, and the powerful impact flow can effectively reduce and eliminate the blocking layer between a solvent and an aqueous phase, thus increasing the mass transfer rate; besides, the impact flow generates a physical shear force towards cell tissues, so that the cell tissues deform, fracture and release inclusions, the cell walls and the interstitial structures of the cell walls suffer from local loosening and swelling, the extraction process of paeonol in the cells is accelerated, and the effect of getting twofold results with half the effort is achieved.

The invention belongs to the technical field of plant biology, and particularly relates to a method for agrobacterium tumefaciens-mediated genetic transformation of dried radix rehmanniae. A leave disc formed in a shearing mode after blades of aseptic dried radix rehmanniae seedlings are unfolded initially and veins are removed serves as the infection receptor of agrobacterium tumefaciens, and a transgenetic seedling of the radix rehmanniae is finally acquired through the processes of agrobacterium infection, co-culture, resistant callus induction, resistance bud initiation, and resistance bud rooting. The method is short in culture period and high in transformation efficiency, successfully achieves genetic transformation of the dried radix rehmanniae, lays the foundation of stable expression of an exogenous gene in the dried radix rehmanniae, and has significance in radix rehmanniae genetic improvement and the molecular biology study.

The invention belongs to the technical field of plant biology, and particularly discloses a regeneration culture medium and a culture method for improving regenerated adventitious buds of echinacea explants. According to the regeneration culture medium, corresponding concentration of DA-6 (diethyl aminoethyl hexanoate) is also especially added besides that 15-60g/L of cane sugar, 3-9g/L of agar, 0.1-1.5mg/L of BA (benzylaminopurine) and 0.01-0.2mg/L of NAA (naphthaleneacetic acid) are added to an MS (Murashige and Skoog) formula aiming at difference of different ploidies and different sources of echinacea explants on the sensitivity to diethyl aminoethyl hexanoate (DA-6). By adopting the regeneration culture medium, the effect of facilitating regeneration of BA-induced adventitious buds is significantly enhanced by adding DA-6. By adopting the regeneration culture medium and the culture method disclosed by the invention, the problem of different genotype limits in regeneration cultivation of echinacea can be effectively overcome; the regeneration efficiency of the adventitious buds is greatly improved; development of relevant research work of biotechnology breeding of the echinacea is facilitated.

The invention belongs to the technical field of plant biology, and particularly discloses a molecular marker for a rice blast resistance gene Pita and application thereof. The molecular marker consists of a pair of outer primers Pita-O-F and Pita-O-R and a pair of inner primers Pita-T-F and Pita-C-R, wherein the primer sequences are shown as SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3 and SEQ ID NO:4. The rice blast resistance gene Pita genotype is authenticated by utilizing the marker; rice blast resistance genes can be genotyped to distinguish a rice blast fungus resistant rice variety from a susceptible variety only through simple PCR without sequencing; auxiliary selection of the molecular marker of a rice blast can be realized; according to the molecular marker disclosed by the invention, the Pita gene detecting efficiency can be improved; the molecular marker is suitable for the molecular marker auxiliary selection of a rice improvement segregation population; the breeding efficiency is improved; the requirement on large-scale molecular breeding is met.

The invention discloses apocarya pollen in-vitro germination liquid culture medium and application thereof in measuring pollen activity. The culture medium uses distilled water as solvent and contains sucrose, H3BO3 and Ca(NO3)2.4H2O. The cultivation method comprises hydration treatment, cultivation temperature and time adjustment. According to the apocarya pollen in-vitro germination liquid culture medium disclosed by the invention, the technical problems that the apocarya pollen germination rate is low and the germination tube growth is slow are solved, so that the pollen germination can be more than 70%. According to the culture method and the cultivation method disclosed by the invention, the pollen germination and growth of the apocarya are improved, the operation is simple, the cost is low, the period is short and the germination rate is high, so that the apocarya pollen in-vitro germination liquid culture medium has an extensive application prospect in the juglandaceae plant biology.

The invention belongs to the technical field of plant biology, and particularly relates to a cherry orchard sod culture method. The cherry orchard sod culture method comprises the following steps of selecting seeds, removing weeds, seeding bahia grass, seeding, fertilizing and watering, cradling and regenerating and seeding astragalus sinicus. By the configuration of a first compound fertilizer and a second compound fertilizer which have functions of nutrition and fermentation, degradation and utilization of herbaceous plant metabolite are benefited, soil environment is further improved, soil fertility is greatly improved, and cherry growing environment is improved. The first compound fertilizer and the second compound fertilizer in which triacontanol is added are applied in the process of orchard sod culture, the bahia grass and the astragalus sinicus are cultured by stages, soil can be guaranteed to be in a sod culture stage all the year around, the capacity of preventing drought and waterlogging of a cherry orchard is improved, the soil fertility of the cherry orchard is not affected, the ecological environment of the cherry orchard is enriched, a large number of bacterial parasites are generated, the number of natural enemies of pests is increased, spreading of the pests is restrained, and a relatively lasting ecological system of the cherry orchard is formed.

The invention relates to a method for culturing anti-soybean-mosaic-virus transgenic plants, and belongs to the technical field of plant biology. According to the method provided by the invention, a sequence of an RNA (ribonucleic acid) fragment shown by SEQ-1 or the sequence complementary with the segment is used. The RNA fragment can be used for interfering the copying and the movement of the soybean mosaic virus so as to inhibit the development of the soybean mosaic virus symptom. The method for culturing the anti-soybean-mosaic-virus transgenic plants provided by the invention comprises the following steps of expressing the RNA molecules in the target plant; obtaining the transgenic plants with the obviously improved resistance on the soybean mosaic virus. The method has an important application value on the culturing of wide-spectrum anti-soybean-mosaic-virus plants.

The invention provides molecular markers for haplotype identification of a paddy rice CMS restoring gene Rf-1 segment and applications thereof, and belongs to the fields of plant biology and molecular breeding. By comparing various types of restorer parent sequencing data of paddy rice, 32 sites of restoring gene Rf-1 segment polymorphism are screened, and a plurality of primer pairs and TaqMan-MGB probe compositions are designed. Sequences of the TaqMan-MGB probe compositions are respectively shown as SEQID NO.1-128. The 32 primer pairs and the TaqMan-MGB probe compositions are further prepared into an OpenArray chip. Haplotype identification of the paddy rice CMS restoring gene Rf-1 segment can be achieved by utilization of the molecular markers or the chip. The molecular markers can be applied for haplotype identification of the CMS restoring gene Rf-1 segment in the paddy rice resource and for breeding of three-line hybrid rice.

The invention relates to a hot pepper pollinator, and belongs to the technical field of plant biology. The pollinator is designed based on the characteristics that hot pepper anther is small, sealed and closed. The pollinator consists of a handle and a pollinating pipe. The making materials are an injector needle head and a pipet suction head; the rear part of the needle head is sleeved at one end of a big head at the top of the pipet suction head; the part of the pipet suction head is used as the handle; and the point part of the needle is used as the pollinating pipe. The pollinator has the advantages that pollen is easy to collect, the pollen storing capacity is high, the pollination area is large and the pollination effect is good.

The invention belongs to the technical field of plant biology and particularly discloses a molecular marker for identifying a genotype of a rice chalkiness major effect gene and application of the molecular marker. The molecular marker consists of a pair of outer primers Chalk5-O-F and Chalk5-O-R and a pair of inner primers Chalk5-C-F and Chalk5-T-R, and a primer sequence is shown in SEQ IDNO:1-4. The marker is moderate in amplified fragments and strong in specificity; when the marker is utilized for identifying the genotype of the rice chalkiness major effect gene Chalk5, gene typing can be performed on the rice chalkiness major effect gene only through simple PCR without sequencing; rice varieties with high and low chalkiness are distinguished, so that molecular marker-assisted selection performed on rice chalkiness characters is realized. The molecular marker disclosed by the invention can be used for the molecular marker-assisted selection of a rice improvement and separation population, improves the breeding efficiency and meets the large-scale molecular breeding requirements.

The invention provides a technology for obtaining peach embryo aseptic seedlings on a large scale, and belongs to the technical field of plant biology. The technology includes the steps of culture medium preparation, peach fruit washing, surface steeping and sterilizing, seed separation, gradient H2O2 solution soaking, sterile water flushing, kernel separation and inoculation, low-temperature processing, room temperature seedling emergence, and the like. The gradient H2O2 solution soaking of seeds serves as the innovative technology characteristic. According to the technology, peroxidase in seed coats and endosperm reacts with H2O2 to release reactive oxygen so that microorganisms possibly carried by the seeds can be killed, and meanwhile the gradient H2O2 solution soaking is beneficial to separation of seed coats and kernels. The technology is easy to operate, high in safety, beneficial for conducting large-scale seed sterilization and seed coat and kernel separation of peach embryo culture on a large scale, the contamination rate is decreased, the inoculation efficiency is improved, and a new method is provided for obtaining the peach embryo aseptic seedlings on a large scale.

The invention belongs to the technical field of plant biology and specifically relates to a method for discriminating the cold resistance of a peony tissue. The method provided by the invention is capable of directly reflecting the adaptability and the buffering ability of the peony tissue to the low-temperature environment and the obvious degree of supercooling by measuring the freezing point temperature and the supercooling temperature of the peony plant tissue part and defining the difference between the freezing point temperature and the supercooling temperature as peony tissue supercooling ability (delta E). The greater the value of delta E is, the more obvious the supercooling phenomenon of the tissue is, the larger the temperature zone sensitive to low-temperature freezing injury is and the higher the adaptability of responding to a sudden low temperature is. The method provided by the invention is simple and convenient, and high in feasibility; and the method is capable of obtaining the supercooling point temperature and the freezing point temperature of the plant tissue from a temperature freezing curve, thereby providing convenient and accurate scientific basis for low-temperature safe storage and low-temperature freeze injury protection of peony.

The invention provides a bone defect repair scaffold, and a construction method and a preparation method thereof, a computer-readable storage medium and equipment. The bone defect repair scaffold includes a bone defect repair scaffold body and a fusion surface layer. The contact surface between the bone defect repair scaffold body and a bone substance is provided with the fusion surface layer. Thebone defect repair scaffold body has a hollow inner cavity, the fusion surface layer is of a porous structure, and the bone defect repair scaffold is made of tantalum metal or tantalum alloy. The bone defect repair scaffold effectively avoids the stress shielding effect and reduces the risk of secondary fracture; tantalum metal or tantalum alloy is a biophilic metal, has no toxic or side effectson a human body, has excellent endophytic biological properties and has the function of promoting the bone defect repair; the porous structure of the tantalum metal or tantalum alloy is conducive to growth of newly regenerated bones into the material and good osseointegration effect is obtained.

The invention provides a molecular marker and application of a rice peel color gene Pb, belonging to the field of plant biotechnology. The molecular marker of the present invention is obtained by PCR amplification of one forward primer and two reverse primers, and the nucleotide sequences of the aforementioned primers are respectively shown in SEQ ID NO.1-3. Using the molecular marker of the rice pericarp color gene Pb, the genotyping of the rice pericarp color gene Pb can be completed by simple PCR, and the rice pericarp color can be predicted. The invention has the advantages of simple operation, rapid typing, low cost and the like, and is more suitable for use in large-scale molecular marker-assisted selection.

The invention belongs to the technical field of plant biology, and particularly discloses a molecular marker and application of a rice blast-resistant gene Pik. The molecular marker comprises a pair of outer primers Pik-O-F and Pik-O-R as well as a pair of inner primers Pik-T-F and Pik-C-R, and primer sequences are shown as SEQ ID NO: 1 to 4. When the molecular marker is used for identifying the genotype of the rice blast-resistant gene Pik, sequencing is not required, and only through simple PCR, genotyping can be performed on the rice blast-resistant gene to distinguish rice blast-resistant rice varieties from rice blast-susceptible varieties, so that molecular marker assistant selection of rice blast is achieved; the molecular marker can improve the detection efficiency of the Pik gene, is applicable to the molecular marker assistant selection of a rice improvement segregation population, can improve the breeding efficiency, and meets the requirement for large-scale molecular breeding.

The invention provides a molecular marker of a rice grain width gene GW2 and an application and belongs to the technical field of plant biology. The molecular marker is obtained through PCR amplification of two forward primers and a reverse primer, wherein nucleotide sequences of the primers are as shown in SEQ ID NO.1-3 separately. By using the molecular marker of the rice grain width gene GW2, genetic typing of GW2 can be completed only through simple PCR, and the rice grain width variation is predicted. The molecular marker has the advantages of being simple in operation, fast in typing, accurate in result and low in cost, is capable of improving the character selection efficiency, and meets the requirements of large-scale molecular marker-assisted selection.

The invention discloses application of tomato gene SISAUR58 in controlling tomato growth and drought resistance. It is discovered through series of experiments discover that by overexpression of SISAUR58 gene in two different tomato genes Micro-Tom and Ailsa Crag, all plants are dwarfed with the quantity and area of leaves significantly reduced and drought resistance improved, while by inhibitingSAUR58, nutrient growth of plants can be accelerated. A method established herein is applicable to tomato, not limited to its growth and resistance control, and is applicable to the increase of plantbiological yield and creation of novel drought-resistant varieties.

The invention belongs to the technical field of plant biology, discloses application of rose RrNUDX1 gene in enhancing of fragrance of plants, and provides a purpose of the rose RrNUDX1 gene in cultivating of new varieties of flowers with rich fragrance. An overexpression vector of the RrNUDX1 gene can be constructed by using the method, and the over expression vector is transferred into to-be-transformed flowers by Agrobacterium mediated methodto obtain the new varieties of transgenic flowers.

The invention discloses a bacteriostatic agent for plant tissue culture and application of bacteriostatic agent in anoectochilus formosanus tissue culture. The bacteriostatic agent for the plant tissue culture comprises the following components in parts by calculating at 1L: 100 to 500 ml of plant extraction liquid, 5 to 20 g of chitosan oligosaccharide, 5 to 20 ml of surfactant and the balance ofwater; and the plant extraction liquid is an ethanol extract solution of a mixture of garlic bulbs, ginger roots, bitter gourd peels and pomegranate peels, and the plant extract liquid and the chitosan oligosaccharide are mixed to obtain the bacteriostatic agent. The bacteriostatic agent can effectively reduce the pollution rate of bacteria and fungus in the tissue culture, can significantly increase the seedling forming rate of the anoectochilus formosanus tissue culture, can reduce the production cost, can be widely applied to the technical field of plant biology such as tissue culture rapid propagation, seedling detoxification, regenerated plant system establishment and the like.

The invention provides a method for culturing rhododendron fortunei polyploidy, and relates to the technical field of plant biology. The method comprises the following steps: sterilizing rhododendronfortunei seeds with oryzalin; transplanting the seeds to a seedling clod for culturing after the seeds are germinated, and detecting the ploidy variation of chromosome by a porosity method and a flowcytometer method; and transplanting the chromosome doubling plant determined by detection into greenhouse for culturing to obtain the polyploidy plant. The rhododendron fortunei polyploidy cultured byadopting the method has high inductivity reaching 30 percent, and high survival rate of variant plants reaching 85 percent or more; and the identifying method is simple and convenient, and can simultaneously prepare massive polyploidy materials, and the breeding efficiency is greatly improved.