Method for extracting paeonol from paeonia suffruticosa roots

Abstract

The invention belongs to the technical field of plant biology and in particular relates to a method for extracting paeonol from paeonia suffruticosa roots. The method comprises the steps of picking, complex enzyme liquid preparation, ultrasonic treatment, centrifugal treatment and reduced pressure distillation. The method has the beneficial effects that enzyme treatment is firstly carried out on paeonia suffruticosa roots and barks at 30 DEG C for 20 minutes, so that the cell walls of the paeonia suffruticosa roots and barks and the interstitial structures of the cell walls can suffer from changes such as local loosening, swelling, collapse and the like; short-time ultrasonic treatment is then carried out on the paeonia suffruticosa roots and barks, ultrasonic waves generate strong impact waves and microjet inside the liquid, and the powerful impact flow can effectively reduce and eliminate the blocking layer between a solvent and an aqueous phase, thus increasing the mass transfer rate; besides, the impact flow generates a physical shear force towards cell tissues, so that the cell tissues deform, fracture and release inclusions, the cell walls and the interstitial structures of the cell walls suffer from local loosening and swelling, the extraction process of paeonol in the cells is accelerated, and the effect of getting twofold results with half the effort is achieved.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, and in particular relates to a method for extracting paeonol from peony roots. Background technique [0002] Peony is a shrub of Paeoniae genus in Ranunculaceae, and it is also known as Luoyang flower, rich flower and "king of flowers". Its root bark is an important traditional Chinese medicine with important medicinal value, and paeonol is an important active component of the root bark. Paeonol is colorless needle crystal, melting point 49°C-51°C, slightly soluble in water, soluble in hot water, insoluble in cold water, volatile with water vapor, soluble in ethanol, ether, acetone, chloroform, benzene and carbon disulfide. Special smell, slightly spicy storage conditions: cool, dry, away from light. Paeonol has analgesic, anti-inflammatory, antipyretic and anti-allergic effects. It has obvious analgesic effect on pain caused by physical or chemical factors such as tail pressure and acetic a...

AI Technical Summary

Problems solved by technology

Under the extraction conditions of 15 MPa and 52 °C, the paeonol extract was a light reddish brown liquid after 2.5 h, and automatically separated into layers after refrigeration. The upper layer was a light yellow liquid, and the lower layer was a light reddish brown solid. The paeonol extracted by the method is less