The invention relates to a method for extracting peony essential oil from peonies. The method comprises the following steps that step 1, the peonies are picked, forced air drying is conducted on the peonies, and the dried peonies are taken out and ground; step 2, a cellulase water solution and a pectinase water solution are mixed, acetum is added, and therefore a phosphoesterases complex solution is obtained; step 3, the ground peonies are placed in a triangular flask, the phosphoesterases complex solution is added, standing is conducted, ultrasonic treatment is conducted, and standing is conducted; step 4, the obtained solution in the step 3 is added to a petroleum ether ethanol solution, backflow is conducted, digestion is conducted at the indoor temperature, and the obtained solution is transferred into a rotary evaporator for vacuum concentration. According to the method, enzyme treatment is firstly conducted on the peonies so that local changes of loosening, expansion, crashing and the like are generated in the cell walls of the peonies and the mesenchyme structures of the cell walls; then, ultrasonic treatment is conducted on the peonies for a short time, and therefore the extraction rate of the essential oil from the cells of the peonies is greatly improved.