Application of rose RrNUDX1 gene in enhancing of fragrance of plants

A gene and plant technology, applied in the direction of angiosperms/flowering plants, applications, plant products, etc., to achieve important economic value, increase commodity value, improve quality and its economic value

Inactive Publication Date: 2017-10-20
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the application of RrNUDX1 gene has not been reported

Method used

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  • Application of rose RrNUDX1 gene in enhancing of fragrance of plants
  • Application of rose RrNUDX1 gene in enhancing of fragrance of plants
  • Application of rose RrNUDX1 gene in enhancing of fragrance of plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0028] 1 Rose RrNUDX1 Gene Transformation in Petunia and Functional Verification

[0029] 1.1 Vector plasmid and strain

[0030] Both the pCAMBIA1304 expression vector and EHA105 Agrobacterium were preserved in our laboratory.

[0031] 1.2 Preparation of main reagents

[0032] (1) YEB liquid medium: 0.05g MgSO 4 ·7H 2 O, 1g yeast extract, 5g peptone, 5g beef extract, 5g sucrose, use ddH 2 O was dissolved and the volume was adjusted to 1000mL, the pH value was adjusted to 7.0, and stored at 4°C after sterilization. YEB solid medium: add 20g / L agar on the basis of YEB liquid medium, sterilize and store in aliquots.

[0033] (2) Kan: mother liquor 50mg / mL. 0.5 g kan, dissolved in 10 mL sterile ddH 2 In O, filter through a 0.22μm filter membrane, aliquot into 1.5mL centrifuge tubes, and store at -20°C.

[0034] (3) Rif: mother solution 5mg / mL. 0.05g Rif, dissolved in a small amount of absolute ethanol and sterilized with ddH 2 Dilute O to 10 mL, filter through a 0.22 μm ...

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Abstract

The invention belongs to the technical field of plant biology, discloses application of rose RrNUDX1 gene in enhancing of fragrance of plants, and provides a purpose of the rose RrNUDX1 gene in cultivating of new varieties of flowers with rich fragrance. An overexpression vector of the RrNUDX1 gene can be constructed by using the method, and the over expression vector is transferred into to-be-transformed flowers by Agrobacterium mediated methodto obtain the new varieties of transgenic flowers.

Description

technical field [0001] The invention discloses the application of rose gene RrNUDX1 in enhancing plant floral fragrance, and belongs to the field of plant biotechnology. Background technique [0002] Rose (Rosa rugosa Thunb.) is an important natural spice plant in the world, and also a traditional medicinal and edible flower in my country. Among the monoterpenes, citronellol, geraniol, nerol and their acetate derivatives It is the main component that forms the characteristic aroma of roses. [0003] As the main species of terpenoids, monoterpenes are mostly distributed in secretory tissues such as glandular oil compartments of plants, and usually have strong aroma and biological activity. Monoterpenes play an important role in plant survival and human life, except for their role in plant defense. In addition to the functions in the plant, monoterpenes are also used as spices and medicines. At the same time, the main components of many valuable plant essential oils are compos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82C12N15/55A01H5/00
CPCC12N15/827C12N9/14
Inventor 冯立国臧姝生利霞魏恬恬
Owner YANGZHOU UNIV
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