41results about How to "Avoid Biosafety Concerns" patented technology

The invention belongs to the technical field of plant biology and particularly relates to separation, function identification and application of a rice anther specific expression promoter. The disclosed promoter is specifically expressed in a plant anther and has good application prospects in the field of plant genetic modification.

The invention discloses a pollen-specific promoter and an expression vector thereof. Experiments show that the promoter can drive efficient and specific expression of a target gene in the pollen of transgenic rice. The promoter of the invention can be used for functional analysis and identification of genes related to growth and development of plant pollen, as well as creation of plant male sterile lines, prevention of plant transgenic drift, and the like. Especially, the promoter has an important application value in creation of male sterile lines of rice and other gramineous crops and utilization of heterozygous advantages.

The invention provides a method for efficiently detecting 15 kinds of bile acid in blood. In the method, serum is directly preserved on a filter paper scrip, and through an efficient extraction process, extracted bile acid is directly injected on a liquid chromatography-mass spectroscopy; 15 kinds of the bile acid in blood can be efficiently detected in a high-throughput and high-repeatability mode, and the method has the wide application value.

The invention discloses a biological mothballing system suitable for synechococcus elongatus, and a construction method and application of the biological mothballing system. The construction method comprises the steps that a virulent gene sepT2, an antitoxic gene sepA2 and an iron-deficiency inducible promoter PisiAB are obtained from the synechococcus elongatus 7942, and a promoter PpsbA2 is obtained from synechocystis 6803; a terminator Trbcl is obtained from integrative plasmid pBA3031 through amplification; pBR322 is taken as a carrier, the virulent gene sepT2 is expressed with the iron-deficiency inducible promoter PisiAB, and the terminator Trbcl is used for terminating transcription; and the antitoxic gene sepA2 is expressed with the promoter PpsbA2, and plasmid is constructed and transferred into the synechococcus elongatus. According to the system, the synechococcus elongatus grows normally under the non-inducing situation and rapidly dies after being induced. The important theoretical and practical significance for development and optimization of the biological mothballing system of the synechococcus elongatus is achieved, and reference is also provided for solving the bio-safety problem of other cyanobacteria.

The invention discloses a method for acquiring gene editing sheep by RNA-mediated specific FGF5 gene knockout and special sgRNA for the method. The sgRNA provided by the invention can realize specific targeted modification of sheep FGF5 gene, is RNA shown in the second to the 21st nucleotides from the sequence 4 to 5' end tail of a sequence table or RNA with the second to the 21st nucleotides from the sequence 4 to 5' tail end of the sequence table. The invention provides an effectively technical tool for sheep functional gene researches.

The invention belongs to the technical field of plant biology and particularly relates to separation, function identification and application of a plant anther specific-expression promoter. The promoter is specifically expressed in plant anther, so that the promoter is excellent in application prospect in the field of plant transgenosis.

The invention provides a self-heating amplification detection device. The self-heating amplification detection device comprises a reaction structure, a detection structure, an intermediate structure, a self-heating structure and a sealing structure. The self-heating amplification detection device can realize self-heating, can perform amplification reaction detection on different samples, and can also improve the safety performance of the device in the reaction process. The invention further provides a preparation method and a use method of the detection device, and the beneficial effects are as described above. The invention further provides a designed nucleic acid detection system, the nucleic acid detection system comprises an extraction device and a detection device, the RPA technology, closed nucleic acid extraction equipment and a self-heating-dependent colloidal gold test card are combined together, so that an extraction reaction system is completely isolated from the external environment, the biological safety problem can be effectively avoided while the detection time is saved by means of closed normal-temperature amplification, nucleic acid detection can be completely separated from large-scale instruments and equipment, and the on-site detection target of sampling while detecting is realized.

The invention belongs to the technical field of plant biology, discloses identification and application of a plant anther specific expression promoter pTaASG005 and particularly relates to separation, function identification and application of the plant anther specific expression promoter. The promoter is specifically expressed in plant anthers, particularly in anthers of pollen in a uninucleate stage, a binucleate stage and a trinucleate stage. The plant anther specific expression promoter pTaASG005 has a promising application prospect in the field of plant transgene.

The invention discloses a promoter with anther tissue specificity. The promoter with the anther tissue specificity is as follows (a) or (b) or (c): (a), 3'end contains at least 1-1698 nucleotide sequences of a sequence 1 in a sequence table, beginning at the 1698 position of sequence 1 and extending to the 3' end of sequence 1 in accordance with the nucleotide sequence of the sequence 1, expandingto the 3' end of the sequence 1 according to the nucleotide sequence of the sequence 1, and any DNA fragment of length from 1698 to 2205bp is obtained; and a DNA molecule has a promoter function; (b)a DNA fragment is provided with a defined identity of 75% or more than 75% of the nucleotide sequence limited by (a), and provided with the promoter function; and (c) a DNA fragment hybridizes with the nucleotide sequence limited by (a) or (b) under strict conditions and is provided with the promoter function. The promoter can be used for the specific expression of exogenous genes in anthers andthe creation of male sterile and restorer lines, and a wide application prospect is achieved.

The invention belongs to the technical field of plant biology and particularly relates to isolation, functional identification and application of a promoter pTaASG019 featuring plant anther-specific expression. The promoter featuring plant anther-specific expression has promising application prospect in the field of plant transgenosis.

The present invention belongs to the field of plant biotechnology, and particularly relates to separation, functional identification and applications of a plant anther-specific expression promoter TaASG046. According to the present invention, the promoter is specifically expressed in the plant anther, and provides good application prospects in the plant transgene field.