Sample target processing system of matrix-assisted laser desorption ionization time-of-flight mass spectrometer

A technology of time-of-flight mass spectrometry and matrix-assisted laser, which is applied in the preparation of test samples, instruments, analytical materials, etc., can solve problems such as reducing the efficiency of microbial mass spectrometry identification, affecting sample stability, and biological safety issues, and ensuring stability. performance, faster drying, and shorter working steps

Pending Publication Date: 2021-02-26
AUTOBIO LABTEC INSTR CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The self-air drying method takes a long time, which reduces the efficiency of microbial mass spectrometry identification; the heating and drying method will cause the sample to decompose and affect the stability of the sample
At the same time, the above two drying methods are in an open environment, and the drying process is likely to cause biological safety problems

Method used

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  • Sample target processing system of matrix-assisted laser desorption ionization time-of-flight mass spectrometer
  • Sample target processing system of matrix-assisted laser desorption ionization time-of-flight mass spectrometer
  • Sample target processing system of matrix-assisted laser desorption ionization time-of-flight mass spectrometer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Such as figure 1 , 2 As shown, the matrix-assisted laser desorption ionization time-of-flight mass spectrometer sample target processing system of the present invention includes a vacuum drying chamber 1 and a vacuum sampling chamber 2; the vacuum drying chamber 1 is used to dry the sample to be detected on the sample target , the vacuum sampling chamber 2 is provided with a moving part for carrying the sample target groove 3, and the sample target groove 3 is used to carry the sample target; the mobile part is preferably selected as a remote control rail car 4, and the remote control rail car 4 is arranged in the vacuum sampling chamber On the track 5 in the chamber 2, the remote control rail car 4 is provided with a lifting mechanism for raising / lowering the sample target groove 3; a transition chamber 6 is provided at the sampling port of the vacuum sampling chamber 2; the transition chamber 6 The formation process is as follows: when the sample target groove 3 move...

Embodiment 2

[0028] Such as image 3 , 4 As shown, the matrix-assisted laser desorption ionization time-of-flight mass spectrometer sample target processing system of the present invention includes a vacuum sampling chamber 1a, and a moving part for carrying a sample target groove 2a is arranged in the vacuum sampling chamber 1a, and the sample target The slot 2a is used to carry the sample target; the mobile part is preferably a remote control rail car 3a, and the remote control rail car 3a is set on the inner rail 4a of the vacuum sample chamber 1a, and the remote control rail car 3a is provided with a slot 2a for raising / lowering the sample target The lifting mechanism; located at the sampling port of the vacuum sampling chamber 1a is provided with a transition chamber 5a that doubles as a vacuum drying chamber, that is, the vacuum drying chamber and the transition chamber in this embodiment are the same chamber. In other embodiments of the present invention, the moving part may have o...

Embodiment 3

[0036] Such as Figure 5 As shown, the difference between this embodiment and Embodiment 2 is that two sample inlets are provided on the side wall of the vacuum sampling chamber 1b, and hatches 2.1b and 2.2b are respectively arranged at the two inlets, corresponding to Specifically, two sample target grooves 3.1b, 3.2b are arranged in the vacuum sampling chamber 1b, and the moving part can independently drive any one of the sample target grooves to move. The air inlet of the mechanical pump 4b is divided into two gas paths, one gas path communicates with the molecular pump 6b through the valve 3.1; The transition chambers 5.1b and 5.2b communicate with the atmosphere through the valve 3.4, the valve 3.5 and the intake filter 7b respectively, and the exhaust port of the mechanical pump 4b communicates with the atmosphere through the exhaust filter 8b.

[0037] In order to avoid the time-consuming drying of the sample target in the transition chamber (the vacuum drying chamber ...

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Abstract

The invention discloses a sample target processing system of a matrix-assisted laser desorption ionization time-of-flight mass spectrometer. The sample target processing system comprises a vacuumizingsystem, a vacuum sample injection chamber provided with a cabin door, and a vacuum drying chamber used for drying a sample target to be tested, wherein a moving part for bearing a sample target groove is arranged in the vacuum sample injection chamber, and the sample target groove is used for bearing a to-be-detected sample target; a transition chamber is arranged at the sample inlet of the vacuum sample injection chamber, and is used for enabling the sample target groove, the sample inlet and the cabin door to form a closed cavity in an enclosing manner when the sample target groove moves tothe sample inlet; an exhaust filter is arranged in a vacuumizing air path of the vacuum drying chamber and is used for filtering harmful impurities in the vacuum drying chamber; and the sample inletis communicated with the atmosphere through a valve. The method has the advantages that mass spectrometric detection can be completed without waiting for the drying of the sample target, so the working steps of mass spectrometric detection are greatly shortened, and meanwhile, the biosafety problem in the drying process is avoided.

Description

technical field [0001] The invention relates to a matrix-assisted laser desorption ionization time-of-flight mass spectrometer, in particular to a sample target processing system for a matrix-assisted laser desorption ionization time-of-flight mass spectrometer. Background technique [0002] Matrix Assisted Laser Desorption Ionization-Time Of Flight Mass Spectrometer (MALDI-TOF MS) is a new type of soft ionization biological mass spectrometer developed in recent years, because of its high sensitivity and accuracy It has been widely used in genomics and proteome research because of its advantages of high precision and high resolution. [0003] In the identification of microbial mass spectrometry, the pretreatment of the sample to be tested is the key factor affecting the success of the identification result, and the operation of adding sample liquid in the pretreatment is an important operation that affects the success of the sample pretreatment. After the sample pretreatmen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/64G01N1/28B01D46/00
CPCB01D46/00G01N1/28G01N27/64
Inventor 李向广尚元贺张亚芳李康康张子奇蔡克亚
Owner AUTOBIO LABTEC INSTR CO LTD
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