Sample target processing system of matrix-assisted laser desorption ionization time-of-flight mass spectrometer

Abstract

The invention discloses a sample target processing system of a matrix-assisted laser desorption ionization time-of-flight mass spectrometer. The sample target processing system comprises a vacuumizingsystem, a vacuum sample injection chamber provided with a cabin door, and a vacuum drying chamber used for drying a sample target to be tested, wherein a moving part for bearing a sample target groove is arranged in the vacuum sample injection chamber, and the sample target groove is used for bearing a to-be-detected sample target; a transition chamber is arranged at the sample inlet of the vacuum sample injection chamber, and is used for enabling the sample target groove, the sample inlet and the cabin door to form a closed cavity in an enclosing manner when the sample target groove moves tothe sample inlet; an exhaust filter is arranged in a vacuumizing air path of the vacuum drying chamber and is used for filtering harmful impurities in the vacuum drying chamber; and the sample inletis communicated with the atmosphere through a valve. The method has the advantages that mass spectrometric detection can be completed without waiting for the drying of the sample target, so the working steps of mass spectrometric detection are greatly shortened, and meanwhile, the biosafety problem in the drying process is avoided.

Description

technical field [0001] The invention relates to a matrix-assisted laser desorption ionization time-of-flight mass spectrometer, in particular to a sample target processing system for a matrix-assisted laser desorption ionization time-of-flight mass spectrometer. Background technique [0002] Matrix Assisted Laser Desorption Ionization-Time Of Flight Mass Spectrometer (MALDI-TOF MS) is a new type of soft ionization biological mass spectrometer developed in recent years, because of its high sensitivity and accuracy It has been widely used in genomics and proteome research because of its advantages of high precision and high resolution. [0003] In the identification of microbial mass spectrometry, the pretreatment of the sample to be tested is the key factor affecting the success of the identification result, and the operation of adding sample liquid in the pretreatment is an important operation that affects the success of the sample pretreatment. After the sample pretreatmen...

AI Technical Summary

Problems solved by technology

The self-air drying method takes a long time, which reduces the efficiency of microbial mass spectrometry identification; the heating and drying method will cause the sample to decompose and affect the stability of the sample

At the same time, the above two drying methods are in an open environment, and the drying process is likely to cause biological safety problems

Images