95 results about "Ionization time of flight" patented technology

The present invention provides for methods of quantitating the amounts of proteins or peptides, including those that are closely related isoforms, using matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). Measurement of protein concentrations in vivo has been extremely difficult and problematic, and protein concentrations have not been shown to correlate well with mRNA levels, the standard used in the past. The present invention overcomes the deficiencies of prior methodologies by taking advantage of MALDI-TOF-MS technology and applying it to proteins and peptides in a way that allows for accurate, quantitative measurement in vivo of protein or peptide concentrations.

The present invention provides a device, system, and associated methods to actively or passively sample air by directing it onto the surface of a porous light-absorbing semiconductor, for example, a desorption/ionization on porous silicon (“DIOS”) chip. Upon adsorption of an analyte, the surface may be analyzed directly by laser desorption/ionization time-of-flight mass spectrometry. Because the process of laser desorption/ionization and subsequent mass detection does not require elevated temperatures, thermal degradation of analytes is avoided.

The present invention provides methods for single nucleotide polymorphism (SNP)-based killer cell immunoglobulin-like receptor (KIR) gene cluster genotyping using the matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometer. In general, the methods involve amplifying a plurality of target sequences of a plurality of KIR genes, and detecting the presence or absence of a plurality of single SNPs of the plurality of KIR genes by MALDI-TOF mass spectrometry. The invention also features compositions, including arrays of capture primers and optionally extension primers on a substrate surface, and kits, for use in the methods of the invention.

The invention relates to a polypeptide immunoassay kit, which comprises a protein G or protein A agarose serving as a solid phase carrier, a purified serum polypeptide antibody and PBS buffer solution. The invention also relates to a polypeptide immunomic spectrometry analysis method, which comprises the following steps: coupling a purified serum antibody with a proper solid carrier; allowing the coupled product to bond with a polypeptide marker antigen specifically, and separating the antigen from the carrier by using eluent; and finally, detecting polypeptide antigen in the eluent by high-pass matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOFMS). Thus, the complete immunomic spectrometry analysis method is established.

Laser desorption/ionization time-of-flight mass spectrometer (“LDI-TOF-MS”) devices, and methods, that accurately measure the mass of analytes contained in a sample and which also measure the quantities of analytes present in a sample in a consistent manner from instrument-to-instrument and over time on a single instrument. In particular, the invention provides LDI-TOF-MS devices and methods in which: 1) the energy of the laser pulse and the area of the sample illuminated (fluence) is consistent and controlled so as to produce consistent conditions for analyte desorption and ionization; 2) the mass analyzer behaves in a reproducible manner; and 3) the detection system produces a signal that consistently represents the arrival of ions of different masses.

The invention relates to a complete-set primer applied to detecting of skin aging related susceptible genes SNP (single-nucleotide polymorphism) and application thereof. The complete-set primer is prepared from amplification primer pairs of 29 SNP sites of 22 skin aging related susceptible genes of human genome DNA and extension primers; a pair of multiple PCR amplification primers and a piece ofsingle-base extension primer are designed for each site respectively, and an MALDI-TOF MS (matrix-assisted laser desorption/ionization time-of-flight mass spectrometry) method is utilized to detect SNP site genotyping of a sample to be detected. The skin aging related susceptible gene detecting method based on gene molecular subtyping disclosed by the invention can finish a plurality of SNP genotyping in the same reaction at the same time, so that accuracy, high efficiency and low cost are achieved. The gene detection disclosed by the invention can be applied to predicting of congenital agingresistance of the skin; thus, a scientific basis is provided for an external skin care scheme.

The invention discloses application of naphthylethylenediamine inorganic acid salt or naphthylethylenediamine organic acid salt as a matrix in MALDI MS (matrix-assisted laser desorption/ionization mass spectrometry). In the application, the MALDI MS is MALDI-TOF MS (matrix-assisted laser desorption/ionization - time-of-flight mass spectrometry); the naphthylethylenediamine inorganic acid salt is naphthylethylenediamine hydrochloride, naphthylethylenediamine nitrate, naphthylethylenediamine phosphate or naphthylethylenediamine sulfate; and the naphthylethylenediamine organic acid salt is naphthylethylenediamine trifluoroacetate, naphthylethylenediamine acetate, naphthylethylenediamine formate, naphthylethylenediamine citrate or naphthylethylenediamine oxalate. The invention technically overcomes the defect that small-molecule samples cannot be effectively analyzed because serious matrix background interference is apt to be caused in a low molecular weight zone when organic small-molecule matrices are commonly used. The matrix used by the invention is not required to be added with ionizing reagent and the requirements on sample treatment are reduced; and since background interference hardly exists within a testing range (m/z: 0-1000), kinds of complex mixed systems can also be analyzed by using the matrix.

A high-power density laser sputtering ionization flying time mass spectrometer and the application thereof relate to a mass spectrometer. A high-power density laser sputtering ionization flying time mass spectrometer and the application thereof are provided. The high-power density laser sputtering ionization flying time mass spectrometer is provided with a vacuum chamber, a laser source, a laser-focusing lens, a sampling cone, an ion transmission device, an interception cone and a flying time mass analyzer; the laser source and the laser-focusing lens are arranged outside the vacuum chamber; besides, the sampling cone, the ion transmission device, the interception cone and the flying time mass analyzer are sequentially arranged in the vacuum chamber. The vacuum chamber is provided with a third-level vacuum chamber; a first-level vacuum chamber is arranged in front of the sampling cone; a second-level vacuum chamber is arranged between the sampling cone and the interception cone; and the third-level vacuum chamber is arranged behind the interception cone. Through the collision with the auxiliary gas molecules (atoms) in the ion source, the kinetic energy of the sample irons is reduced to facilitate sampling and transmission; the multivalent ions lose valences and are converted into polyvalent ions to remove the interference peak of multivalent ions, so that the spectrum is clear and easy to read and has strong distinguishing ability.

The invention discloses a complete-set primer for SNP (Single Nucleotide Polymorphism) sites of drug-metabolizing enzyme genes and application thereof. The complete-set primer comprises amplificationprimers and extension primers of 9 SNP sites of 7 drug-metabolizing enzyme associated genes of human genome DNA, wherein a pair of multiple PCR amplification primers and a single-base extension primerare respectively designed for each site, and an MALDI-TOF MS (Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry) method is adopted to detect typing of the SNP sites of a sample to be detected. The complete-set primer disclosed by the invention has the beneficial effects that a detection method for the drug-metabolizing enzyme genes based on gene molecule typing is established, and the typing of multiple SNPs can be finished at the same time in the same reaction, so that the accurate and efficient effects are achieved and the cost is low; the covered genes and sitesare related to the metabolizing enzyme genes related to 8 major types of common drugs for children, and the coverage is most complete in gene detection products of safe drugs used for children so far.

The invention discloses a matrix with selectivity for micromolecule MALDI-TOF MS (Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry) detection and an application thereof. The matrix is graphene oxide grafted 4-vinylbenzeneboronic acid, and takes 4-vinylbenzeneboronic acid as a monomer; the 4-vinylbenzeneboronic acid is grafted to graphene oxide which is subjected to bromine functionalization by triggering atom transfer radical polymerization reaction on the surface, thereby preparing the matrix. The graphene oxide grafted 4-vinylbenzeneboronic acid disclosed by the invention can act as the MALDI-TOF MS matrix for selectively recognizing such micromolecule compounds containing cis-form adjacent dihydroxy structure, as cis-form adjacent dihydroxy compounds in saccharides, phenols and nucleoside; the minimum detection limit can reach 1pmol/mL around, and the selectivity and flexibility are both very high.

The invention discloses a protein fingerprint model formed through drawing according to mass-to-charge ratios of a plurality of proteins and protein crest strength coefficients, wherein the mass-to-charge ratios are 1097, 1777, 2551, 2988, 3381, 3708, 5492, 6007, 7144 and 8012. The invention further discloses a protein detection and analysis system based on the model and a laser desorption/ionization time of flight mass spectrometry detection method for anthrax. In the detection method, protein spectrums of samples are compared with the model to detect the anthrax in the samples qualitatively. The protein fingerprint model contains specific protein spectrum information of the anthrax, is provided with a specific recognition function, and can be applied to commercialized detection and analysis systems of the anthrax. According to the protein fingerprint model, the system and an application thereof for the anthrax, the detection method which is high in sensitivity, specificity and detection rate is provided for the anthrax and is particularly suitable for port check and inspection departments.

The present invention discloses a mobile surveillance car-based single particle volatile organic compound online mass spectrum detection system and method, the system includes a mobile surveillance car and a volatile organic compound online mass spectrometer, the interior of the mobile surveillance car is provided with a driving room, a workspace area, a functional area and an instrument area, and the volatile organic compound online mass spectrometer is a single-photon ionization flight time mass spectrometer, is placed in the functional area, and is used for qualitative and quantitative real-time online analysis of industrial source volatile organic compound emissions. The mobile surveillance car-based single particle volatile organic compound online mass spectrum detection system can detect the composition and concentration of industrial source volatile organic compounds, a sample can be directly and quickly detected without sample preprocessing, high throughput sample analysis can be achieved, pollutant source volatile organic compound emissions can be detected in crusing mode, and the emissions of the composition and concentration of the volatile organic compounds at higher time resolution over time can be characterized.

The invention discloses a kit used for detecting body size traits of chicken and a molecular breeding method of the chicken and belongs to the field of molecular genetics. MassARRAY system matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and a single-primer extension reaction are combined, miRNA (micro ribonucleic acid)-1658 (rs16681031 C/G) single-SNP (single nucleotide polymorphism) site genotyping detection on F2 generation resource population samples of Gushi chicken and Anak chicken is performed, and correlation analysis with the size traits of F2 generation resource populations is performed. Results show that the chicken miRNA-1658 gene SNP (single nucleotide polymorphism) is obviously correlated with the body size traits of the chicken; a GG gene facilitates obvious increasing of chest breadth, crista breadth and body slanting length of a four-week-year-old chick; chicken flocks with excellent genetic resources can be quickly built by taking the GG gene as assistant selection of the body size trait of the four-week-year-old chick and as a molecular genetic breeding marker of the four-week-year-old chick.

The invention provides a novel method for checking the polymorphism of mononucleotide by a combination of a special restriction endonuclease and a mass spectrometric method. Key points of the invention are as follows: a primer taking part in the PCR reaction contains recognition sites and endonuclease sites of different positions of the special restriction endonuclease, and PCR products undergo the restriction endonuclease digestion, which generates a single chain nucleotide segment containing SNP sites; after processes of purification and point target, etc., a sample which can be directly used for the substrate auxiliary laser analytic ionization flight time mass spectrometric detection is prepared. Compared with the prior art, the novel method has the advantages of short time, low cost, high resolution and convenient and easy operation.

The invention provides a 96-well high-throughput solid-phase extraction device for mass spectrographic analysis. The device comprises a silicone seal cover plate, joint extraction column holes, a 96-well joint extraction column plate, an upper sieve plate, a lower sieve plate, a solid-phase extraction column packing, a 96-well sample collection plate and sample collection holes. According to the extraction device, a 96-well solid-phase extraction plate packed with the corresponding packing is utilized, a vacuum negative-pressure device or gravity centrifugation is connected, which is used for extracting high-throughput protein or polypeptide from the body fluid or biological sample lysate and directly used for matrix-assisted laser desorption ionization time-of-flight mass spectrometry and electrospray ionization mass spectrometry. By the extraction device, the solutions including protein or polypeptide extracted from multiple body fluid or biological sample lysate can be directly used for mass spectrometric detection without extra steps including drying, collection, dissolution and so on. During the whole process, the operation is simple and convenient, rapid and high-throughput. The extraction device has excellent application value in the pretreatment of high-throughput biological sample of mass spectrometry.

A mass spectrometer probe is formed of a nonconductive polymer that is doped with conductive material. The probe may be used as, or as part of, a repeller plate in a parallel laser ion desorption/ionization time-of-flight mass spectrometer. Transparent locations on the probe enable a sample placed thereon to be visualized before or during mass spectrometry. The conductive nature of the probe maintains the consistency of the electromagnetic field applied to the sample. The probe also displays low outgassing and high mechanical and chemical stability, thereby enabling it to be used repetitively.

The invention provides an on-line self-cleaning method for a vacuum ultraviolet lamp, and belongs to the technical field of performance optimizing and maintaining of single-photon ionization time-of-flight mass spectrometers. The obtained method is designed for the problem that the light intensity is damped after the vacuum ultraviolet lamp is used for a long term. The method comprises the steps that when the light intensity is damped caused by the fact that a vacuum ultraviolet lamp window is polluted or oxidized by organic matter, oxyhydrogen with a certain concentration is pumped into the surface of the lamp window under on-line medium vacuum, and normal proceeding of a test is not affected; due to the strong reducing property of hydrogen, the surface of the oxidized lamp window can be recovered in a certain degree; in addition, the oxyhydrogen can generate hydroxyl radical, the hydroxyl radical reacts with hydrocarbon adhered to the surface of the lamp window to obtain the volatile organic matter to be off from the lamp window. Based on the above steps, on-line self-cleaning of the vacuum ultraviolet lamp is achieved, a device does not need to be disassembled, normal proceeding of the test is not affected, no interference on a collected spectrogram exists, and the advantages of being convenient to operate, simple in method, stable and reliable in performance and long in service life are achieved.

The invention discloses a kit for detecting chicken growth characters and a chicken molecular breeding method, which belong to the field of molecular genetics. According to the kit and the chicken molecular breeding method, a Mass ARRAY system matrix-assisted laser desorption/ionization time-of-flight mass spectrometry technique is combined with single primer extension reaction, so as to perform miRNA-1687 (rs15179830G/T) single SNP locus genetic typing on Gushi-Anak F2 generation hybrid chickens and perform association analysis on the growth characters of the F2 generation hybrid chickens, results indicate that chicken miRNA-1687 gene SNP (single nucleotide polymorphism) is significantly correlated to chicken weight at different developmental stages, TT type individual weight is significantly superior to GT type individual weight and GG type individual weight, and a chicken group with good genetic resources can be quickly established by using the TT genotype as assistant selection of the chicken weight and a molecular genetic breeding marker.

The present invention to a protein related to carcinoma of urinary bladder and its detection method. Said invention adopts surface intensified laser desorption ionization time-of-flight mass spectra technology, combined application protein chip technology and Biomarker Patterns Software to create tree analysis mode for detecting and analyzing the specific protein related to the carcinoma of urinary bladder which has difference expression in urine sample. The sensibility of the protein related to the tested carcinoma of urinary bladder can be up to 84%, and its specificity can be up to 91%.