Protein related to carcinoma of urinary bladder and detection method thereof

A technology related to proteins and detection methods, applied in biological testing, material testing products, peptide sources, etc., can solve the problems of low cytology sensitivity, limited clinical value, subjective interference, etc., achieve great clinical value, reduce medical treatment, etc. Expenses, high sensitivity effects

Inactive Publication Date: 2004-03-24
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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Problems solved by technology

However, the sensitivity of urine exfoliation cytology is low, only about 60%, especially for low-grade tumors, which has

Method used

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  • Protein related to carcinoma of urinary bladder and detection method thereof
  • Protein related to carcinoma of urinary bladder and detection method thereof
  • Protein related to carcinoma of urinary bladder and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1) Preparation of urine

[0020] Three groups of fresh urine samples were taken, respectively, the urine sample group (46 cases) of patients diagnosed with bladder cancer clinically and pathologically, and the urine sample group of patients with other benign diseases of the urinary system (such as benign prostatic hyperplasia, urinary system inflammation, ureteral stones, etc.) The liquid sample group (32 cases) and the urine sample group (40 cases) of healthy volunteers, the latter two groups were the control group (72 cases in total), and the samples were centrifuged and packaged at -80°C for later use.

[0021] 2) Preparation of chips

[0022] (1) Chip pretreatment:

[0023] Add 10 μl of CuSO4 100Mm (Sigma Company) solution to each sampling point, put it in a wet box for 10 minutes, then take it out and spin dry. Repeat the operation once. Put the chip into a test tube of deionized water, shake it, take it out and shake it dry. Add 10 μl of sodium acetate 50mM pH...

Embodiment 2

[0043] Blind Screening Application of Dendritic Analysis Model HS-BL-329

[0044] Three groups of 155 urine samples were used, including 118 samples for pattern establishment and another 37 urine samples. Among them, there were 61 cases in the bladder cancer group, 42 cases in the other benign urinary system disease group, and 53 cases in the healthy volunteer group. Among the 37 urine samples, there were 14 cases in the bladder cancer patient group, 10 cases in the patient group with other benign urinary system diseases, and 13 cases in the healthy volunteer group. Using the same experimental conditions as in Example 1 and the same software processing parameters, the obtained protein profiles were screened using the HS-BL-329 tree model analysis software. The results showed that the sensitivity of the above tree analysis model to detect 155 samples was 80.3%. , the specificity can reach 86.3%. And other benign diseases of the urinary system in the group 2 can be distinguish...

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Abstract

The present invention to a protein related to carcinoma of urinary bladder and its detection method. Said invention adopts surface intensified laser desorption ionization time-of-flight mass spectra technology, combined application protein chip technology and Biomarker Patterns Software to create tree analysis mode for detecting and analyzing the specific protein related to the carcinoma of urinary bladder which has difference expression in urine sample. The sensibility of the protein related to the tested carcinoma of urinary bladder can be up to 84%, and its specificity can be up to 91%.

Description

technical field [0001] The invention belongs to the field of biology and medical examination, and relates to a tumor biomarker and a detection method thereof, in particular to a bladder cancer-related protein and a detection method thereof. Background technique [0002] In recent years, with the rapid development of genomics research and proteomics, scientists have realized that genomics research needs to be supplemented by protein-level research data. It has been reported that a method for studying proteins (proteomic pattern diagnosis) uses surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF-MS) protein chip system to analyze proteins. This method overcomes the limitations of traditional protein analysis. It enables rapid analysis of the quality and functional properties of all proteins in blood, urine and other body fluids, cells or tissues in a small amount of crude extraction samples. SELDI is a mass spectrometry technology based on...

Claims

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Application Information

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IPC IPC(8): C07K14/435G01N33/68
Inventor 吕元关明刘维薇张万忠吴忠张元芳
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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