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Kit for detecting chicken growth characters and chicken molecular breeding method

A technology for molecular breeding and growth traits, applied in the field of molecular genetics, which can solve the problems of insignificant differences and unfavorable selection and retention.

Inactive Publication Date: 2015-09-09
NANYANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in this method, the miRNA-1704SNP has no significant difference between the birth weight, 2-week-old body weight, and 4-week-old body weight of the chicken, and the difference is significant from the 6-week-old age. Considering that the current growth cycle of intensive chicken farming is About 50 days, this method is not conducive to early selection and retention

Method used

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  • Kit for detecting chicken growth characters and chicken molecular breeding method
  • Kit for detecting chicken growth characters and chicken molecular breeding method
  • Kit for detecting chicken growth characters and chicken molecular breeding method

Examples

Experimental program
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Effect test

Embodiment 1

[0036] The kit for detecting chicken growth traits in the present embodiment includes:

[0037] 10pmol / μl upstream primer F PRimer: 5'-ACGTTGGATGTGAACAGCAACACAGCTAGG-3',

[0038] 10pmol / μl downstream primer R PRimer: 5'-ACGTTGGATGAGCAACTTCTTTGCTGGCTG-3';

[0039] 7 μM single base extension primer: 5'-AGTGACTGCAGCATAAAAA-3'.

Embodiment 2

[0041] In this embodiment, the kit used to detect chicken growth traits, in addition to the following primers, also includes: 10 × buffer, 25mM Mg 2+ , 25mM dNTP, 5U / μl Hotstar, the reaction concentration is 1×iPLEX stop reaction Mix, the reaction concentration is 1×iPLEX single base elongase, SAP buffer, 1.7U / μl SAP enzyme, ddd H 2 O and DNA Marker;

[0042] 10pmol / μl upstream primer F PRimer: 5'-ACGTTGGATGTTCTGCCATACCAGTGTGTG-3',

[0043] 10pmol / μl downstream primer R PRimer: 5'-ACGTTGGATGACTCCTCCAGCTGCTGCTTC-3';

[0044] 7 μM single base extension primer: 5'-CATCAACACCAACCCCA-3'.

Embodiment 3

[0117] The molecular breeding method based on chicken miRNA-1687 gene SNP in the present embodiment may further comprise the steps:

[0118] 1) Genomic DNA extraction and PCR amplification

[0119] The chicken peripheral blood DNA was extracted by the phenol chloroform isoamyl alcohol method, dissolved in an appropriate amount of TE (pH 8.0), stored at -20°C for later use, and used for PCR amplification with primers F Primer / R PRimer to obtain the amplified product;

[0120] Upstream primer F PRimer: 5'-ACGTTGGATGTGAACAGCAACACAGCTAGG-3',

[0121] Downstream primer R PRimer: 5'-ACGTTGGATGAGCAACTTCTTTGCTGGCTG-3';

[0122] The reaction system of PCR amplification is: total 5.0μl; 10×buffer 0.5μl, 25mM Mg 2+ 0.4μl, 25mM dNTP0.1μl, 5U / μl Hotstar 0.2μl, 10pmol / μl F Primer / R Primer 0.5μl each, 100ng / μl total DNA 1.0μl, ddd H 2 O 1.8 μl;

[0123] The reaction program of PCR amplification is: pre-denaturation at 95°C for 2min; 95°C for 30s; 56°C for 30s; 72°C for 60s, 45 cycles; 72...

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Abstract

The invention discloses a kit for detecting chicken growth characters and a chicken molecular breeding method, which belong to the field of molecular genetics. According to the kit and the chicken molecular breeding method, a Mass ARRAY system matrix-assisted laser desorption / ionization time-of-flight mass spectrometry technique is combined with single primer extension reaction, so as to perform miRNA-1687 (rs15179830G / T) single SNP locus genetic typing on Gushi-Anak F2 generation hybrid chickens and perform association analysis on the growth characters of the F2 generation hybrid chickens, results indicate that chicken miRNA-1687 gene SNP (single nucleotide polymorphism) is significantly correlated to chicken weight at different developmental stages, TT type individual weight is significantly superior to GT type individual weight and GG type individual weight, and a chicken group with good genetic resources can be quickly established by using the TT genotype as assistant selection of the chicken weight and a molecular genetic breeding marker.

Description

technical field [0001] The invention relates to a kit for detecting growth traits of chickens, and also relates to a molecular breeding method based on the single nucleotide polymorphism of chicken miRNA-1687 gene, which belongs to the field of molecular genetics. Background technique [0002] With the rapid development of social economy and the improvement of people's living standards, the market's demand for meat quality has generally shifted from the previous quantity requirement to the pursuit of quality and flavor. Growth traits, body size traits, meat quality traits and carcass traits are the most economical traits in the poultry industry today. Most economic traits are quantitative traits, which are mainly determined by a large number of micro-effect genes. These genes have additive effects, dominant effects and epistasis effects at the same time. The genetic basis of complex traits is the genetic factors that affect the phenotype. It is jointly determined by environ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/686C12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/178C12Q2565/627C12Q2533/101
Inventor 石建州赵金兵
Owner NANYANG NORMAL UNIV
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