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Matrix with selectivity for micromolecule MALDI-TOF MS (Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry) detection and application thereof

A selective, small-molecule technology, applied in measurement devices, analytical materials, material analysis by electromagnetic means, etc., can solve problems such as limited applications, and achieve the effect of highly sensitive selective detection

Inactive Publication Date: 2014-05-07
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, graphene or graphene oxide will polymerize during the synthesis and application process, which severely limits its application

Method used

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  • Matrix with selectivity for micromolecule MALDI-TOF MS (Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry) detection and application thereof
  • Matrix with selectivity for micromolecule MALDI-TOF MS (Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry) detection and application thereof
  • Matrix with selectivity for micromolecule MALDI-TOF MS (Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry) detection and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Disperse 0.3g of 4-vinylbenzeneboronic acid in 15mL of 1,4-dioxane, then add 0.1g of bromine-functionalized graphene oxide, sonicate for 10 minutes, and then add 0.044g of cuprous bromide under nitrogen protection and 0.044g of pentamethyldivinyltriethylamine, stirred at room temperature for 24 hours, after the reaction was complete, washed with methanol:acetic acid (4:1), deionized water, and acetone for several times to remove the reagents that did not react completely, and finally 30 °C vacuum drying. Graphene oxide grafted 4-vinylbenzene boronic acid is obtained.

[0021] The prepared graphene oxide grafted 4-vinylbenzene boronic acid was characterized by MALDI-TOF MS, and the results are shown in figure 1 . It can be seen from the figure that it acts as a matrix and has few impurity peaks in the low molecular weight region, so it is very beneficial for the detection of small molecules.

Embodiment 2

[0023] Disperse 0.1g of 4-vinylbenzeneboronic acid in 15mL of 1,4-dioxane, then add 0.1g of bromine-functionalized graphene oxide, sonicate for 10 minutes, and then add 0.02g of cuprous bromide under nitrogen protection and 0.02g of pentamethyldivinyltriethylamine, stirred at room temperature for 24 hours, after the reaction was complete, washed with methanol:acetic acid (4:1), deionized water and acetone for several times to remove the reagents that were not fully reacted, and finally 30 °C vacuum drying. Graphene oxide grafted 4-vinylbenzene boronic acid is obtained.

Embodiment 3

[0025] Disperse 0.5g of 4-vinylbenzeneboronic acid in 15mL of 1,4-dioxane, then add 0.1g of bromine-functionalized graphene oxide, sonicate for 10 minutes, and then add 0.06g of cuprous bromide under nitrogen protection and 0.06g of pentamethyldivinyltriethylamine, stirred at room temperature for 24 hours, after the reaction was complete, washed with methanol:acetic acid (4:1), deionized water and acetone for several times to remove the reagents that did not react completely, and finally 30 °C vacuum drying. Graphene oxide grafted 4-vinylbenzene boronic acid is obtained.

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PUM

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Abstract

The invention discloses a matrix with selectivity for micromolecule MALDI-TOF MS (Matrix-Assisted Laser Desorption / Ionization Time of Flight Mass Spectrometry) detection and an application thereof. The matrix is graphene oxide grafted 4-vinylbenzeneboronic acid, and takes 4-vinylbenzeneboronic acid as a monomer; the 4-vinylbenzeneboronic acid is grafted to graphene oxide which is subjected to bromine functionalization by triggering atom transfer radical polymerization reaction on the surface, thereby preparing the matrix. The graphene oxide grafted 4-vinylbenzeneboronic acid disclosed by the invention can act as the MALDI-TOF MS matrix for selectively recognizing such micromolecule compounds containing cis-form adjacent dihydroxy structure, as cis-form adjacent dihydroxy compounds in saccharides, phenols and nucleoside; the minimum detection limit can reach 1pmol / mL around, and the selectivity and flexibility are both very high.

Description

technical field [0001] The invention belongs to the technical field of detection of cis-ortho-dihydroxy compounds, and particularly relates to surface-induced atom transfer radical polymerization to synthesize graphene oxide grafted 4-vinylbenzene boronic acid and its detection of cis-ortho-dihydroxy compounds by MALDI-TOF MS Applications. Background technique [0002] In the late 1980s, a new type of soft ionization biological mass spectrometry, matrix-assisted laser desorption / ionization time-of-flight mass spectrometry (MALDI-TOF MS), was developed, which has the characteristics of good accuracy, high sensitivity and high resolution. MALDI-TOF MS is a simple and fast technique mainly used to analyze large biomolecules, so it has been widely used to analyze proteins, peptides, nucleotides, etc. It simplifies the sample preprocessing steps and saves a lot of labor. [0003] In MALDI-TOF MS, the matrix functions to absorb and transmit laser energy, ionizing the sample. Ho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62
Inventor 张静郑晓玲倪艳丽
Owner SHAANXI NORMAL UNIV
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