96-well high-throughput solid-phase extraction device for mass spectrometric analysis and application thereof

A mass spectrometry analysis and extraction device technology, which is applied in the field of mass spectrometry devices

Active Publication Date: 2017-06-30
HANGZHOU WELL HEALTHCARE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the large sample volume required by the traditional extraction cartridges, it is impossible to meet the requirements of clinical proteom

Method used

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  • 96-well high-throughput solid-phase extraction device for mass spectrometric analysis and application thereof
  • 96-well high-throughput solid-phase extraction device for mass spectrometric analysis and application thereof
  • 96-well high-throughput solid-phase extraction device for mass spectrometric analysis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Fabrication of a 96-hole high-throughput solid-phase extraction device for mass spectrometry analysis

[0025] see Figure 1-2 , a 96-hole high-throughput solid-phase extraction device for mass spectrometry provided by the present invention, the main structure of the 96-hole high-throughput solid-phase extraction device is shown in figure 1 , mainly composed of silica gel sealing cover plate 1, row extraction column hole 2, 96-hole row extraction column plate 3, upper sieve plate 4, solid phase extraction column packing 5, lower sieve plate 6, 96-hole sample collection plate 7, sample Composed of 8 collection holes, the extraction column holes 2 are arranged in a row on the 96-hole extraction column plate 3, the extraction column holes 2 are open at both ends, the silica gel sealing cover plate 1 and the 96-hole extraction column plate 3 pass through the silica gel Particles are sealed, and after sealing, the pressure difference between the inside and outside...

Embodiment 296

[0029] Example 2 Application of 96-hole high-throughput extraction device in vacuum desiccator

[0030] The 96-well strip extraction column plate 3 filled with the solid phase extraction column filler 5 is placed on the 96-well sample collection plate 7, and the buffer solution or sample solution is added to the strip extraction column hole 2 of the 96-well strip extraction column plate 3 In the process, cover the 96-well extraction column plate 3 tightly with a silica gel sealing cover plate 1, put the assembled whole 96-well high-throughput extraction device into the vacuum desiccator 9, connect the vacuum pump to the vacuum pump connection port 10 of the vacuum desiccator, and pump vacuum, such as image 3, the solution quickly flows through the solid phase extraction column filler 5. Take out the 96-well sample collection plate 7, discard the solution or collect the extract. Re-add buffer or sample solution to well 2 of the strip extraction column, and repeat the above p...

Embodiment 396

[0031] Example 3 Application of a 96-hole high-throughput extraction device in a bottom-sealed vacuum device

[0032] The 96-well strip extraction column plate 3 filled with the solid phase extraction column filler 5 is placed on the 96-well sample collection plate 7, and the buffer solution or sample solution is added to the strip extraction column hole 2 of the 96-well strip extraction column plate 3 , and put into the bottom sealing vacuum device 12, after the rubber or silica gel sealing ring 13 of the bottom sealing vacuum device seals the 96-hole extraction column plate 3, the vacuum pump is connected to the vacuum pump connection port 11 of the sealing vacuum device to evacuate, as Figure 4 ; Flow through the solid phase extraction column packing 5. Take out the 96-well sample collection plate 7, discard the solution or collect the sample extract. Re-add buffer or sample solution to well 2 of the strip extraction column, and repeat the above process until the protein ...

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PUM

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Abstract

The invention provides a 96-well high-throughput solid-phase extraction device for mass spectrographic analysis. The device comprises a silicone seal cover plate, joint extraction column holes, a 96-well joint extraction column plate, an upper sieve plate, a lower sieve plate, a solid-phase extraction column packing, a 96-well sample collection plate and sample collection holes. According to the extraction device, a 96-well solid-phase extraction plate packed with the corresponding packing is utilized, a vacuum negative-pressure device or gravity centrifugation is connected, which is used for extracting high-throughput protein or polypeptide from the body fluid or biological sample lysate and directly used for matrix-assisted laser desorption ionization time-of-flight mass spectrometry and electrospray ionization mass spectrometry. By the extraction device, the solutions including protein or polypeptide extracted from multiple body fluid or biological sample lysate can be directly used for mass spectrometric detection without extra steps including drying, collection, dissolution and so on. During the whole process, the operation is simple and convenient, rapid and high-throughput. The extraction device has excellent application value in the pretreatment of high-throughput biological sample of mass spectrometry.

Description

technical field [0001] The invention belongs to a mass spectrometry analysis device, and relates to a 96-hole high-throughput solid-phase extraction device for mass spectrometry analysis. The device can be used for protein extraction of biological samples and directly for mass spectrometry detection. Background technique [0002] Clinical proteomics mass spectrometry detection has been widely used in disease research. Since proteins, not nucleic acids, are the specific executors and manifestations of life activities, the study of disease pathogenesis and the search for specific and sensitive disease markers objectively require further exploration at the level of the proteome. According to technology and research purposes, tumor proteomics can be divided into expression proteomics and functional proteomics. Expression proteomics includes two-dimensional gel electrophoresis (2-DE), chromatography combined with mass spectrometry, peptide and protein microarrays, tissue arrays ...

Claims

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Application Information

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IPC IPC(8): G01N27/64G01N1/34
CPCG01N1/34G01N27/64
Inventor 余捷凯郑树袁瑛
Owner HANGZHOU WELL HEALTHCARE TECH CO LTD
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