96-well high-throughput solid-phase extraction device for mass spectrometric analysis and application thereof

Abstract

The invention provides a 96-well high-throughput solid-phase extraction device for mass spectrographic analysis. The device comprises a silicone seal cover plate, joint extraction column holes, a 96-well joint extraction column plate, an upper sieve plate, a lower sieve plate, a solid-phase extraction column packing, a 96-well sample collection plate and sample collection holes. According to the extraction device, a 96-well solid-phase extraction plate packed with the corresponding packing is utilized, a vacuum negative-pressure device or gravity centrifugation is connected, which is used for extracting high-throughput protein or polypeptide from the body fluid or biological sample lysate and directly used for matrix-assisted laser desorption ionization time-of-flight mass spectrometry and electrospray ionization mass spectrometry. By the extraction device, the solutions including protein or polypeptide extracted from multiple body fluid or biological sample lysate can be directly used for mass spectrometric detection without extra steps including drying, collection, dissolution and so on. During the whole process, the operation is simple and convenient, rapid and high-throughput. The extraction device has excellent application value in the pretreatment of high-throughput biological sample of mass spectrometry.

Description

technical field [0001] The invention belongs to a mass spectrometry analysis device, and relates to a 96-hole high-throughput solid-phase extraction device for mass spectrometry analysis. The device can be used for protein extraction of biological samples and directly for mass spectrometry detection. Background technique [0002] Clinical proteomics mass spectrometry detection has been widely used in disease research. Since proteins, not nucleic acids, are the specific executors and manifestations of life activities, the study of disease pathogenesis and the search for specific and sensitive disease markers objectively require further exploration at the level of the proteome. According to technology and research purposes, tumor proteomics can be divided into expression proteomics and functional proteomics. Expression proteomics includes two-dimensional gel electrophoresis (2-DE), chromatography combined with mass spectrometry, peptide and protein microarrays, tissue arrays ...

AI Technical Summary

Problems solved by technology

However, due to the large sample volume required by the traditional extraction cartridges, it is impossible to meet the requirements of clinical proteomics with high throughput and rapid realization of a small number of large samples, rapid batch operation, and direct use in mass spectrometry detection.

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