168 results about "Electrospray mass spectrometry" patented technology

The invention is directed to multiply-substituted fullerene derivatives of novel configurations, and methods for their preparation and use. The methods involve the combinatorial synthesis of a library of fullerene derivatives and comprises the steps of forming a mixture of fullerene derivatives by reacting the Cn fullerene with two or more reactive precursor compounds, and removing the unreacted compounds to yield the fullerene derivatives having the desired activity. Methods for the identification and screening of a combinatorial library of fullerenes by 3He-nuclear magnetic resonance and electrospray mass spectrometry to define members with the optimal desired activity are also provided.

The present invention provides a method for rapid solution capture purification of nucleic acids for subsequent analysis by electrospray mass spectrometry which is efficient and cost-effective relative to existing methods. The present invention also provides for kits useful for practicing rapid solution capture of nucleic acids so that purified samples are in condition for analysis by electrospray mass spectrometry.

The invention discloses a multi-channel micro-fluidic chip and mass spectrum combined device. The multi-channel micro-fluidic chip and mass spectrum combined device comprises a micro-fluidic chip and an electric spraying mass spectrometer. The micro-fluidic chip is provided with a plurality of micro channels which are connected in parallel, and the outlet of each micro channel is communicated with a chamber. Each chamber is communicated with a main channel, and the other end of each main channel is a waste liquid outlet. One end of each main channel is close to a chamber and is communicated with a side channel, and the free end of the side channel is an extraction agent inlet. Height difference exists between the side channel and the main channel, and the upper rim of the side channel is arranged on the lower portion of the upper rim of the main channel. Sample outlets are communicated with sample outlet pipes respectively, wherein the outlets of the sample outlet pipes are arranged above the electric spraying device, and liquid drops generated by the electric spraying device is detected by the electric spraying mass spectrometer. A plurality groups of experiments can be simultaneously conducted by the multi-channel micro-fluidic chip and mass spectrum combined device, and real-time mass spectrum detection can be conducted after on-line sample pretreatment is conducted in the micro-fluidic chip.

The present invention provides a method for rapid solution capture purification of nucleic acids for subsequent analysis by electrospray mass spectrometry which is efficient and cost-effective relative to existing methods. The present invention also provides for kits useful for practicing rapid solution capture of nucleic acids so that purified samples are in condition for analysis by electrospray mass spectrometry.

An inexpensive electrospray mass spectrometer capable of performing measurements consecutively from the ESI mode to the cold-spray ionization mode and vice versa. The electrospray mass spectrometer has an electrospray ion source, a nebulization nozzle, and a sampling orifice. The axes of the nozzle and orifice intersect each other. The instrument has a movable cold-spray desolvation chamber. In the electrospray ionization mode, the desolvation chamber is placed off the axis of the nebulization nozzle. In the cold-spray ionization mode, the desolvation chamber is set on the axis of the nebulization nozzle.

A human glucagon associated peptide-2 analog Pro-Pro-h[Gly2]GLP-2(1-35) for treating the short bowel syndrome and malabsorption of stomach and intestine is prepared through configuring genetic engineering bacterium for effective expression of Pro- Pro-h[Gly2]GLP-2(1-35) in colibacillus cell, splitting, washing, dissolving urea, depositing in alcohol, separating fusion protein, hydrolyzing it, chromatography by DEAE-52 column, separating by HPLC, and freeze drying.

[The invention teaches the uses of a plurality of electric fields and of orthogonal spray configurations of vaporized analyte which combine so as to operate to enhance the efficiency of analyte detection and mass analysis with a mass spectrometer by reducing vapor in the vacuum system and concomitant noise. Several embodiments of the invention are described for purposes of illustration.] The invention relates to a method and apparatus for improving signal relative to noise without loss of ion collection efficiency in electrospray mass spectrometry, including liquid chromatography / mass spectrometry.

The invention discloses a capillary needle. The capillary needle comprises a tube body, the two ends of the tube body are respectively provided with an inlet and an outlet for electro-spray, a part of the tube body is bent to form a sampling end, and the sampling end is provided with a sampling port communicating with a cavity in the tube body. The invention also discloses an electro-spray ionization mass spectrometry analytical apparatus applying the capillary needle. The invention also simultaneously discloses a method applying the electro-spray ionization mass spectrometry analytical apparatus for analytical detection. The analytical apparatus is high in integration, compact in structure, easy to control, small in size and low in cost; the sample consumption is greatly reduced, and the experiment cost is decreased; an integrated sample introduction method is employed, and an sample introduction end does not have to be repeatedly placed in a sample and blank solution, such that cross contamination is reduced, and more importantly, the analytical flux is substantially improved; and through combination with an automatic sample changing system, different samples can be automatically introduced, and therefore, the capillary needle, the apparatus and the method are quite suitable for such fields as high-flux screening, unicell analysis, mass spectrum imaging analysis and the like.

The invention belongs to the field of chemistry, and relates to a microextraction probe electrospray ion source and a manufacturing method and application of the microextraction probe electrospray ion source, in particular to a probe capable of being directly used for complex-matrix solid-phase microextraction and direct-electrospray mass spectrometry analysis and a manufacturing method and application of the probe. The probe is characterized in that a silanization reaction is carried out on adsorption materials containing silylation and pointed-shaped wood fiber matrixes with the surface rich in hydroxyl, and the microextraction probe is obtained. By means of the probe, high enrichment coefficients of trace target compounds in multiple kinds of complex matrixes can be directly extracted, the probe after extraction can carry out direct electrospray to ionize the target compounds in the normal-pressure open environment and carry out mass spectrometry analysis; the microextraction electrospray probe achieves solid-phase microextraction and ambient mass spectrometry combination analysis, can be used as the novel electrospray ion source and has the high sensitivity and the ideal reproducibility.

The charge state of ions produced by electrospray ionization is reduced in a controlled manner to yield predominantly singly charged ions through reactions with bipolar ions generated using a <210>Po alpha particle source or equivalent. The multiply charged ions generated by the electrospray undergo charge reduction in a charge reduction chamber. The charge-reduced ions are then detected using a commercial orthogonal electrospray TOF mass spectrometer, although the charge reduction chamber can be adapted to virtually any mass analyzer. The results obtained exhibit a signal intensity drop-off with increased oligonucleotide size similar to that observed with MALDI mass spectrometry, yet with the softness of ESI and without the off-line sample purification and pre-separation required by MALDI.

Affinity Capture-Mass Spectroscopy (AC-MS), an analytical technique which couples the sensitivity of a label-free binding detected biosensor, and the information richness of mass spectroscopy is described. A 3-dimensional porous silicon bio-surface is used to capture proteins, DNA, or small molecules while acquiring a label-free, time resolved signal linearly proportional to the amount of binding. A switch to dissociative buffer conditions then frees the captured molecule for analysis by mass spectroscopy. In particular, techniques for use with electrospray mass spectroscopy are described.

Novel and significantly simplified procedures for fabrication of fully integrated nanoelectrospray emitters have been described. For nanofabricated monolithic multinozzle emitters (NM2 emitters), a bottom up approach using silicon nanowires on a silicon sliver is used. For microfabricated monolithic multinozzle emitters (M3 emitters), a top down approach using MEMS techniques on silicon wafers is used. The emitters have performance comparable to that of commercially-available silica capillary emitters for nanoelectrospray mass spectrometry.

The present invention provides for a structure comprising a plurality of emitters, wherein a first nozzle of a first emitter and a second nozzle of a second emitter emit in two directions that are not or essentially not in the same direction; wherein the walls of the nozzles and the emitters form a monolithic whole. The present invention also provides for a structure comprising an emitter with a sharpened end from which the emitter emits; wherein the emitters forms a monolithic whole. The present invention also provides for a fully integrated separation of proteins and small molecules on a silicon chip before the electrospray mass spectrometry analysis.

The invention provides a determination method by liquid chromatography-electrospray mass spectrometry (LC-ESI-MS / MS) for detecting various phthalate esters in milk simultaneously, belonging to the field of food detection technology. The milk sample is directly extracted by acetonitrile and sodium chloride; a formic acid ammonium acetate solution-carbinol system is taken as a flowing phase for liquid chromatography separation so as to carry out gradient elution; and the quantitative detection is carried out by a mass spectrum detector by adopting the peak area method of an external standard method. The method has high sensitiveness, good exactness and preciseness, reliable specialization and authenticity, is extremely simple and convenient and easy to be applied to practical detection, and can meet the requirement in practical detection excellently.

The present invention is a method to determine the TAN and TAN as a function of boiling point for a hydrocarbon feedstream using an electrospray ionization mass spectrometer (ESI-MS). The steps of the method include determining the signal as a function of mass from the ESI-MS while minimizing the formation of oligomers and fragmentation of the molecular species in the feedstream and then determining the TAN from the signals. The TAN is also determined as a function of boiling point.

An improved process for processing the raw aconite root in order to decrease its poison and increase its curative effect includes such steps as immersing in water, drying in the air, and high-temp baking to obtain prepared aconite root.

An alternating current electrospray mass spectrometry device includes an electrospray device having at least one emitter providing a passageway for transmission of an analyte sample. At least one conductive element is in electrical communication with the at least one emitter. A power source generates an alternating current electric field to form a liquid cone at a tip of the emitter and ionizes the analyte sample present in the liquid cone. The frequency of the electric field entrains low mobility ions in the liquid cone. The AC electric field causes the emitter to discharge the liquid cone as a liquid aerosol drop, and a mass spectrometry device analyzes the ionized analyte sample to determine the composition of the contained analyte sample.

An inexpensive electrospray mass spectrometer capable of performing measurements consecutively from the ESI mode to the cold-spray ionization mode and vice versa. The electrospray mass spectrometer has an electrospray ion source, a nebulization nozzle, and a sampling orifice. The axes of the nozzle and orifice intersect each other. The instrument has a movable cold-spray desolvation chamber. In the electrospray ionization mode, the desolvation chamber is placed off the axis of the nebulization nozzle. In the cold-spray ionization mode, the desolvation chamber is set on the axis of the nebulization nozzle.

Fluidic separation devices and methods with reduced sample broadening are provided. A column is located downstream from a holding chamber, and a flow providing means provides fluid flow effective to convey a sample along a flow path that extends from the holding chamber into the separation column. The sample is typically focused in the flow path upstream from the separation column. Optionally, the invention may be employed with electrospray mass spectrometry and in microfluidic applications.

The invention relates to a method for packing a separation column and a nozzle needle integrated capillary column and manufacturing a stopper, which comprises the following steps: preparing a chromatography packing into a homogenate with a certain concentration; reversely pressing the homogenate into the capillary column to pack by nitrogen under a certain pressure, wherein one end of the capillary column is a tapered taper; sintering the chromatography packing at the mouth of the taper to form the stopper; and during the process of sintering, using a stainless steel tube to control the lengthof the stopper and protect the chromatography packing at the back of the stopper. The method for packing the separation column and the nozzle needle integrated capillary column and manufacturing thestopper is simple and fast to operate without special equipment and high in success ratio. The capillary column packed according to the method has high separation efficiency and good separation reproducibility; and the stopper manufactured according to the invention has small volume, no sample adsorption and no post-column effect. The method for packing the separation column and the nozzle needleintegrated capillary column and manufacturing the stopper has very good application value in the fields of protein sciences, micro environments and biological sample analyses based on capillary liquidchromatography-electrospray ionization mass spectrometry technology.

Novel and significantly simplified procedures for fabrication of fully integrated nanoelectrospray emitters have been described. For nanofabricated monolithic multinozzle emitters (NM2 emitters), a bottom up approach using silicon nanowires on a silicon sliver is used. For microfabricated monolithic multinozzle emitters (M3 emitters), a top down approach using MEMS techniques on silicon wafers is used. The emitters have performance comparable to that of commercially-available silica capillary emitters for nanoelectrospray mass spectrometry.

The invention provides a method for inspecting quality of indigowoad leaf Chinese medicament, which comprises the following steps: processing indigowoad leaves by sample preprocessing means such as water extraction, ethanol processing, extraction with ethyl acetate and water saturated n-butyl alcohol sequentially, concentration an volume fixation; obtaining a finger-print of the indigowoad leaf Chinese medicament by electron spray mass spectrometry; measuring the nucleoside content and purine content of the indigowoad leaf Chinese medicament by high performance liquid chromatography; and measuring the total flavonoid content of the indigowoad leaf Chinese medicament by UV-VIS spectrophotometry, and integrating the finger-print and the measure data of the content to evaluate the quality of the indigowoad leaf Chinese medicament. The quality inspection method is accurate, quick, convenient and effective.

The invention relates to antibacterial peptides and a preparation method thereof. An amino acid sequence of the antibacterial peptide is IleTrpArgIlePheArgArgIlePhe. The method comprises the following steps of: 1) designing a group of antibacterial peptides consisting of isoleucine, arginine and phenylalanine which serve as basic units by utilizing the bacteriostatic mechanism of the antibacterial peptides and the characteristic of amino acid composition; 2) synthesizing a polypeptide crude product by using a polypeptide synthesizer in a solid-phase synthetic process; 3) purifying the synthesized polypeptide by reversed phase high performance liquid chromatography, and identifying by electrospray mass spectrometry; and 4) performing an in-vitro activity test, wherein a result of the activity test indicates that antibacterial peptides B containing 3 basic units have high bacteriostatic activity and weak hemolytic activity, a treatment index is the highest, and cell selectivity is optimum, so the antibacterial peptides have high application value.

The invention provides a single-cell mass spectrometric analysis system and a single-cell mass spectrometric analysis implementation method. The system comprises a first micropositioner, a microscope,a second micropositioner, a first image collection component and a mass spectrometer which are sequentially arranged, wherein a cell locating chip is arranged on an objective table of the microscope;a liquid adding needle is arranged on the first micropositioner and is suitable for adding an extract liquor into the cell locating chip; and a liquid taking needle is arranged on the second micropositioner and is suitable for sucking extract obtained through extraction on the extract liquor and conveying the extract into the mass spectrometer. By use of the system, high-precision, high-speed, high-repeatability and high-automation mass spectrometric detection analysis on a single cell is realized, and the analysis quantity can reach a picoliter level. Meanwhile, inherent defects in the manual operation process can be overcome, mass spectrometric single-cell analysis gets rid of dependency on an operator, the process is more normalized, the system is easier and more convenient to use, analysis is more precise, and a result is more reliable.

The invention provides a constructing method of an electrospray ionization mass spectrometry fingerprint of Guangdong herbal tea granules and a standard fingerprint, and relates to a quality control method of a traditional Chinese medicine preparation, in particular to a method for establishing the electrospray ionization mass spectrometry fingerprint to achieve quality evaluation and source distinguishment of the Guangdong herbal tea granules. The method comprises the following steps: (1) preparation of a test solution; (2) mass spectrometry condition: electrospray ionization mass spectrometry is directly used for detection, mass spectrometry contour map is recorded in a full-scanning mode, and scanning quality range m / z is 100-1000; (3) detection: the fingerprint is directly obtained by the electrospray ionization mass spectrometry; (4) standard fingerprint: the fingerprint of a test product uses the fingerprint according to the Similarity Evaluation System for Chromatographic Fingerprint of TCM(2004 A edition); and the standard fingerprint is generated through calculation using a mean value method; and (5) main ingredient analysis: main ingredients of the fingerprint of the test product are analyzed by using statistics software, so as to evaluate the quality and distinguish sources. The constructing method of an electrospray ionization mass spectrometry fingerprint of the Guangdong herbal tea granules, provided by the invention, is beneficial for monitoring product quality and has the advantages as follows: the quality of the Guangdong herbal tea granules is rapidly, efficiently and reliably represented; simple and convenient method, stability, high sensitivity and good repetitiveness are achieved; requirements on high throughput analysis are satisfied; and the quality of a product can be rapidly and accurately identified.

The invention relates to an isotopic abundance detection method for D, 13C or 15N labeled organic compounds. A high performance liquid chromatograph mass spectrometer is used to obtain the mass spectrometry data of a target compound, and the spectrometry data is analyzed through a ''quality cluster'' classification calculation method, so as to realize the isotopic abundance detection of D, 13C or 15N labeled organic compounds. The detection objects are D, 13C or 15N labeled organic compounds. The detection instrument is a high performance liquid chromatograph mass spectrometer, and the liquid chromatography part is used as an automatic sampling device to achieve high throughput sample introduction; an electro-spray ionization mass spectrometry is used as a detector to obtain mass data acquisition. A ''quality cluster '' classification method is used as a calculation method, and through the analysis of the distribution of mass distribution of various ''quality clusters'' on the contribution of the mass distribution of the target compound (mass spectrum data of target compound), the mole fraction of each ''cluster quality'' is calculated, so as to calculate the isotope labeling abundance of the target compound.