Single-cell extraction electrospray mass spectrometric analysis system and method

A mass spectrometry, single-cell technology, applied in the field of single-cell extraction electrospray mass spectrometry system, can solve the problems of difficulty in removing the mutual interference of different components, affecting the detection of target components, insufficient stability, etc., and achieves high repeatability and results. Reliable, process-specific results

Active Publication Date: 2020-02-07
TSINGHUA UNIV
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Problems solved by technology

However, they rely on researchers to manually control tiny sampling needles, which are difficult to operate and lack stability
And because the cytoplasm is very viscous and has many components, including various substances such as proteins, lipids, nucleotides, inorganic

Method used

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  • Single-cell extraction electrospray mass spectrometric analysis system and method
  • Single-cell extraction electrospray mass spectrometric analysis system and method
  • Single-cell extraction electrospray mass spectrometric analysis system and method

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Embodiment 1

[0140] use Figure 1-5 Lung cancer cells are analyzed by single-cell mass spectrometry analysis system, in which, the inner diameter of the liquid injection needle tip is 15 microns, the cone angle is less than 5°, the inner diameter of the liquid collection needle tip is 2 microns, the cone angle is 15°, and the capture hole diameter is 30 microns.

[0141] The A549 lung cancer cell sample is transferred to a single-cell localization chip, and single cells are captured in the localization units distributed in the array on the chip. Then the cells are lysed by drying, and after the cells are lysed, the single-cell positioning chip is placed on the motorized stage, and the automatic single-cell picoliter extraction operation software (such as Figure 18 ), adjust the stage so that the sample is placed directly above the eyepiece, select an objective lens of appropriate magnification (10× objective lens is used here for the A549 of 10-15m level), and adjust the focal plane of th...

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PUM

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Abstract

The invention provides a single-cell mass spectrometric analysis system and a single-cell mass spectrometric analysis implementation method. The system comprises a first micropositioner, a microscope,a second micropositioner, a first image collection component and a mass spectrometer which are sequentially arranged, wherein a cell locating chip is arranged on an objective table of the microscope;a liquid adding needle is arranged on the first micropositioner and is suitable for adding an extract liquor into the cell locating chip; and a liquid taking needle is arranged on the second micropositioner and is suitable for sucking extract obtained through extraction on the extract liquor and conveying the extract into the mass spectrometer. By use of the system, high-precision, high-speed, high-repeatability and high-automation mass spectrometric detection analysis on a single cell is realized, and the analysis quantity can reach a picoliter level. Meanwhile, inherent defects in the manual operation process can be overcome, mass spectrometric single-cell analysis gets rid of dependency on an operator, the process is more normalized, the system is easier and more convenient to use, analysis is more precise, and a result is more reliable.

Description

technical field [0001] The invention relates to the field of instrument detection and analysis. Specifically, the present invention relates to a single cell extraction electrospray mass spectrometry system and method. Background technique [0002] Currently, single-cell analysis is getting more and more attention. In the field of biomedicine, such as gene analysis, drug development, tissue formation, cancer mechanism, disease treatment, etc., it is necessary to analyze single cells. In the traditional research of cell biology, people take population cells as the research object, and have explored the pathogenic mechanism and treatment methods of many diseases. However, with the development of life sciences, researchers began to gradually realize that there are differences between individual cells. This difference is called "single-cell heterogeneity", which plays a crucial role in many life processes. character of. In the clinical treatment of cancer, after a group of ca...

Claims

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Application Information

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IPC IPC(8): G01N27/62G01N1/34
CPCG01N27/62G01N1/34
Inventor 王文会赵鹏梁非丰泳翔
Owner TSINGHUA UNIV
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