81 results about "Cell localization" patented technology

An imaging fountain flow cytometer allows high resolution microscopic imaging of a flowing sample in real time. Cells of interest are in a vertical stream of liquid flowing toward one or more illuminating elements at wavelengths which illuminate fluorescent dyes and cause the cells to fluoresce. A detector detects the fluorescence emission each time a marked cell passes through the focal plane of the detector. A bi-directional syringe pump allows the user to reverse the flow and locate the detected cell in the field of view. The flow cell is mounted on a computer controlled x-y stage, so the user can center a portion of the image on which to zoom or increase magnification. Several computer selectable parfocal objective lenses allow the user to image the entire field of view and then zoom in on the detected cell at substantially higher resolution. The magnified cell is then imaged at the various wavelengths.

The invention discloses an integrated microfluidic chip applied to localization, culture and interaction of different cells. The chip is composed of a flow layer, a control layer and a covering layer from up down; wherein, the flow layer is mainly composed of a micron pipe network and four round cavities, every two adjacent cavities are connected by a small pipe, and the flow layer is provided with ten sample inlet / outlets in total; the control layer is composed of a plurality of enclosed micro valve switches at the terminal of single pipe and can control time and space of input and output of samples at different pipe positions of the flow layer. The integrated microfluidic chip of the invention has the advantages of good quality of preparation material, strong repeatability of preparation method and wide application range and can be used for research on localization co-culture and interaction of multiple cells.

The present invention discloses a composite nanometer structure electrochemical cell sensor, which comprises two assemblies of a multi-functional mixed nanometer probe and a nanometer structure electrode interface, wherein the multi-functional mixed nanometer probe (HRP-TRAIL-Fe3O4@Au) for electrochemical cell sensing comprises Au nanoparticle-modified magnetic Fe3O4 nanometer spheres immobilized with recombinant human TRAIL protein and horseradish peroxidase (HRP) through a co-immobilization effect, and the nanometer structure electrode interface is an electrode cell sensing interface constructed through a layer-by-layer assembling method, wherein the nanometer structure electrode interface integrates high biocompatibility Au nanoparticles (AuDSNPs) stabilized by a dendrimer, high resistivity nitrogen-doped carbon nano-tubes (CNx) and a high specificity cellular localization oligonucleotide aptamer, and has a nanometer layered structure. The composite nanometer structure electrochemical cell sensor can be provided for carrying out selective quantification detection on expression of DR4 / DR5 death receptors on leukemic cells and surfaces thereof. The present invention further discloses the preparation method.

A phosphorescent transition metal complex especially suitable as sensor for hydrogen peroxide in cells, in particular in mitochondria in live cells, includes a metallic central atom, which is a transition metal, a first ligand with at least one pyridine ring, and a second ligand having at least one pyridine ring and at least one structural component selected from the group of 1,2-diketone moiety and an arylboronate moiety. A method for preparing the metal complex is also disclosed as well as a method of detecting hydrogen peroxide in cells by incubating cells with the transition metal complex. The metal complex shows advantageous intense and long-lived phosphorescence accompanied by exceptional cellular localization properties. The metal complex is highly photostable and highly selective towards hydrogen peroxide, thus, especially suitable for detecting mitochondrial H2O2.

With the goal of creating a combination vaccine against Shigella and other diarrheal pathogens we have constructed a prototype vaccine strain of Shigella flexneri 2a (SC608) that can serve as a vector for the expression and delivery of heterologous antigens to the mucosal immune system. SC608 is an asd derivative of SC602, a well-characterized vaccine strain, which has recently undergone several phase 1 and 2 trials for safety and immunogenicity. Using non-antibiotic asd-based plasmids, we have created novel constructs for the expression of antigens from enterotoxigenic E. coli (ETEC), including CFA / I (CfaB and CfaE) and the B-subunit from heat-labile enterotoxin (LTB) in Shigella vaccine strain SC608. Heterologous protein expression levels and cellular localization are critical to immune recognition and have been verified by immunoblot analysis. Following intranasal immunization (SC608(CFAI) and SC608(CFAI / LTB) of guinea pigs, serum IgG and IgA immune responses to both the Shigella LPS and ETEC antigens can be detected by ELISA. In addition, ELISPOT analysis for ASCs from cervical lymph nodes and spleen showed similar responses. All vaccine strains conferred high levels of protection against challenge with wild-type S. flexneri 2a using the Sereny test. Furthermore, serum from guinea pigs immunized with SC608 expressing CfaB and LTB contained antibodies capable of neutralizing the cytological affects of heat-labile toxin (HLT) on Chinese Hamster Ovary (CHO) cells. These initial experiments demonstrate the validity of a multivalent invasive Shigella strain that can serve as a vector for the delivery of pathogen-derived antigens.

The invention discloses a method for detecting the location of resveratrol in target cells. Specifically, the method comprises the following steps of: firstly pretreating the target cells with resveratrol based on the autofluorescence property of resveratrol; then treating the target cells by using a specifically fluorescently-labeled subcellular organelle probe; and finally determining the distribution of resveratrol in the subcellular organelle of the target cells according to the overlap state of the autofluorescence of resveratrol and the fluorescence of the subcellular organelle probe in the cells. The method disclosed by the invention can be used for detecting the distribution of resveratrol in target cells, and has wide application prospect.

The invention relates to phosphatase ligands and polyligands. In particular, the invention relates to ligands, homopolyligands, and heteropolyligands that modulate PP1 activity. The ligands and polyligands are utilized as research tools or as therapeutics. The invention includes linkage of the ligands, homopolyligands, and heteropolyligands to a cellular localization signal, epitope tag and / or a reporter. The invention also includes polynucleotides encoding the ligands and polyligands.

The invention provides recombinant DNA molecules and constructs useful for providing efficient transgene sub-cellular localization of proteins in transgenic plants. Recombinant DNA molecules and constructs for conferring herbicide tolerance or resistance to plants are further provided, as well as plants exhibiting herbicide tolerance and methods for producing or utilizing such plants.