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83 results about "Cell localization" patented technology
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High resolution imaging fountain flow cytometry
InactiveUS7161665B2Improve throughputHigh resolutionWithdrawing sample devicesMaterial analysis by optical meansHigh resolution imagingFlow cell
An imaging fountain flow cytometer allows high resolution microscopic imaging of a flowing sample in real time. Cells of interest are in a vertical stream of liquid flowing toward one or more illuminating elements at wavelengths which illuminate fluorescent dyes and cause the cells to fluoresce. A detector detects the fluorescence emission each time a marked cell passes through the focal plane of the detector. A bi-directional syringe pump allows the user to reverse the flow and locate the detected cell in the field of view. The flow cell is mounted on a computer controlled x-y stage, so the user can center a portion of the image on which to zoom or increase magnification. Several computer selectable parfocal objective lenses allow the user to image the entire field of view and then zoom in on the detected cell at substantially higher resolution. The magnified cell is then imaged at the various wavelengths.
Owner:UNIVERSITY OF WYOMING
Microfluidic chip for capturing and identifying circulating tumor cells
ActiveCN106754240AAvoid lostHigh precisionBioreactor/fermenter combinationsBiological substance pretreatmentsControl layerFiltration
The invention provides a microfluidic chip for capturing and identifying circulating tumor cells. The microfluidic chip comprises a three-layer structure sequentially stacked and sealed to each other, wherein the three-layer structure comprises a microvalve control layer, a microvalve film layer and a substrate with multiple functional areas from top to bottom, respectively; the micro-valve control layer is provided with a hole penetrating through the control layer for the sample inflow and outflow, and a gas passage for the gas introduction; the micro-valve film layer can bend to deform under the control of pressure in a thin film layer area corresponding to the gas passage; and the substrate contains three functional areas, respectively an impurity filtration area, a cell separation area and a single cell localization and fluorescence identification area. The three functional areas are connected by three fluid passages, and the opening and closing of each fluid passage are controlled by a separate gas microvalve. According to the invention, the capture, identification and automatic counting of circulating tumor cells in the blood of cancer patients are realized, and an effective means is provided for analysis of the circulating tumor cells.
Owner:THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
Imaging cell sorter
InactiveUS20150132766A1Accurate analysisImage enhancementBioreactor/fermenter combinationsCell sorterFluorescence
The present invention provides a cell enrichment / purification device having a function of continuously enriching cells, a function of locating the cells in a particular area of a flow path in a continuous array after the cell enrichment, a function of recognizing the shape of the cells and fluorescence emission from the cells at the same time in units of one cell based on an image, and a function of recognizing the cells based on the information on the shape and fluorescence emission to separate / purify the cells.
Owner:ON CHIP CELLOMICS CONSORTIUM +1
System for and method of positioning cells and determing cellular activity thereof
InactiveUS20060129043A1Enhance affinity adherenceAvoid componentsNanomedicineLaboratory glasswaresCellular targetingEngineering
A device for positioning at least one cell in at least one addressable position, the device comprising a substrate (12) formed with at least one addressable pore (14) and at least one channel (16) embedded in the substrate and being held in fluid communication with the at least one pore. The at least one pore and the at least one channel are designed and constructed such that an under-pressure formed in the at least one channel results in vacuum adherence of the at least one cell onto the at least one pore, such that a single cell is vacuum adhered onto a single pore. In one embodiment, the substrate is a non-conductive substrate and is further formed with one or more electrode structures (22), where each of the electrode structures is positioned in one of the pores. In an additional embodiment the device is designed to be locatable on an organ, such as the brain.
Owner:RAMOT AT TEL AVIV UNIV LTD
Expression cloning processes for the discovery characterization, and isolation of genes encoding polypeptides with a predetermined property
InactiveUS6197502B1Protein production is suppressedSugar derivativesMicrobiological testing/measurementBinding siteRapid identification
The present invention is directed to a method for detection, characterization and isolation of nucleic acids encoding proteins of a desired property, such as a particular cellular localization. The invention further provides for rapid expression of such proteins or glycoproteins in mammalian cells and for facilitated purification of the novel secreted proteins or glycoproteins. Further, the invention provides for radioactive labelling of the novel proteins or glycoproteins, for rapid identification of sites of binding including animals and for rapid production of infective viral vectors for use in gene transfer.
Owner:CYTOS BIOTECHNOLOGY AG
Integrated microfluidic chip and application thereof in living cell control and analysis
InactiveCN101597568AGood biocompatibilityImprove insulation performanceMicrobiological testing/measurementTissue/virus culture apparatusControl layerEngineering
The invention discloses an integrated microfluidic chip applied to localization, culture and interaction of different cells. The chip is composed of a flow layer, a control layer and a covering layer from up down; wherein, the flow layer is mainly composed of a micron pipe network and four round cavities, every two adjacent cavities are connected by a small pipe, and the flow layer is provided with ten sample inlet / outlets in total; the control layer is composed of a plurality of enclosed micro valve switches at the terminal of single pipe and can control time and space of input and output of samples at different pipe positions of the flow layer. The integrated microfluidic chip of the invention has the advantages of good quality of preparation material, strong repeatability of preparation method and wide application range and can be used for research on localization co-culture and interaction of multiple cells.
Owner:NORTHWEST A & F UNIV
Unicell detector based on nano fiber probe and its probe manufacturing method
ActiveCN103105353AIncrease success rateEnhanced interactionNanosensorsIndividual particle analysisPhoton detectionNanoring
The invention relates to an unicell detector based on a nano fiber probe, which comprises a nano probe, a light source unit, a micro operation system, an electricity detection unit, a cellular localization system and a photon detection unit, an innermost layer of the nano probe is a fiber layer, the outer wall of the fiber layer is wrapped with a nano ring electrode layer, and the outer wall of the nano ring electrode layer is wrapped with an insulating layer. A manufacturing method of the unicell detector based on the nano fiber probe comprises the steps of drawing, sputtering, making the insulating layer and cutting focused ion beam. The unicell detector has high sensitivity and can realize the unicell grade detection, compared with a traditional detection means by crushing millions of cells, the required cell sample amount is greatly reduced, and the success rate of disease detection at early stage can be increased. The unicell detector can perform in-vivo cell detection and can avoid the false appearance generation during a cell processing process. The space-time resolution and detectable target object scope can be greatly enhanced, and a biochemistry mechanism in the unicell enables real-time detection and analysis.
Owner:SOUTHWEST UNIVERSITY
Immunohistochemical PD-L1 membrane staining pathological section image processing method, device and equipment
The invention relates to an immunohistochemical PD-L1 membrane staining pathological section image processing method, device and equipment. The image processing method comprises the following steps: acquiring a digital section full-field image of a to-be-diagnosed immunohistochemical PD-L1 (SP263) membrane staining pathological section; adopting a region segmentation network to identify and segment a tumor cell region in the digital slice full-field image under the first visual field multiplying power to obtain a tumor cell region probability graph of the whole digital slice full-field image;identifying and segmenting cells in each digital slice full-field graph, taking the tumor cell region probability graph as a weight matrix to carry out region constraint on the cell positioning network, identifying cell characteristics on the digital slice full-field graph, and positioning and classifying various cells on the digital slice full-field graph; and marking the cell position, the celltype and the immunohistochemical PD-L1 (SP263) index on the full-field diagram of the digital slice. By designing a multi-level feature collaborative diagnosis strategy, the tumor proportion score isaccurately evaluated in a mode of constraining cell features by using regional features.
Owner:杭州迪英加科技有限公司 +1
Single-cell extraction electrospray mass spectrometric analysis system and method
ActiveCN110763752AHigh precisionHigh speedPreparing sample for investigationMaterial analysis by electric/magnetic meansCell extractionCell mass
The invention provides a single-cell mass spectrometric analysis system and a single-cell mass spectrometric analysis implementation method. The system comprises a first micropositioner, a microscope,a second micropositioner, a first image collection component and a mass spectrometer which are sequentially arranged, wherein a cell locating chip is arranged on an objective table of the microscope;a liquid adding needle is arranged on the first micropositioner and is suitable for adding an extract liquor into the cell locating chip; and a liquid taking needle is arranged on the second micropositioner and is suitable for sucking extract obtained through extraction on the extract liquor and conveying the extract into the mass spectrometer. By use of the system, high-precision, high-speed, high-repeatability and high-automation mass spectrometric detection analysis on a single cell is realized, and the analysis quantity can reach a picoliter level. Meanwhile, inherent defects in the manual operation process can be overcome, mass spectrometric single-cell analysis gets rid of dependency on an operator, the process is more normalized, the system is easier and more convenient to use, analysis is more precise, and a result is more reliable.
Owner:TSINGHUA UNIV
Chip for implementing cellular localization culture based on single-cell capture and using and preparation method thereof
InactiveCN106047706AAccurate captureHigh single cell rateEpidermal cells/skin cellsArtificial cell constructsAdherent CultureCultured Cell Line
The invention relates to a single-cell capture and localization culture chip based on a micro-processing technology and a using and preparation method thereof and belongs to the field of bio-micro-electromechanical systems (MEMS). The chip is composed of a cellular localization culture layer and a slide layer. The cellular localization culture layer comprises a sample inlet 1, a sample outlet 8 and a plurality of cellular localization culture units between the sample inlet 1 and the sample outlet 8. When the chip is used, first cell suspension is added from the sample inlet 1, and cells enter a capture area 3 in an array; culture solution containing no cell is added from a sample outlet 7, captured cells are subjected to fluid thrust and enters a cell culture area 5 along a channel, and the cells undergo adherent culture in a culture structure. According to the chip, the contradiction between a small space needed for cell capture and a large space needed for cell culture spreading is solved, the cell capture area and the cell culture area are integrated on the chip, the cells can be captured accurately to obtain a high single cell rate, and an enough spreading space can be provided for single cells so that the cells can grow adherently.
Owner:NORTHWESTERN POLYTECHNICAL UNIV
Nanometer structure electrochemical cell sensor preparation method, produced nanometer structure electrochemical cell sensor and use thereof
InactiveCN103713028AImprove hydrophilicityGood biocompatibilityMaterial electrochemical variablesAptamerNucleotide
The present invention discloses a composite nanometer structure electrochemical cell sensor, which comprises two assemblies of a multi-functional mixed nanometer probe and a nanometer structure electrode interface, wherein the multi-functional mixed nanometer probe (HRP-TRAIL-Fe3O4@Au) for electrochemical cell sensing comprises Au nanoparticle-modified magnetic Fe3O4 nanometer spheres immobilized with recombinant human TRAIL protein and horseradish peroxidase (HRP) through a co-immobilization effect, and the nanometer structure electrode interface is an electrode cell sensing interface constructed through a layer-by-layer assembling method, wherein the nanometer structure electrode interface integrates high biocompatibility Au nanoparticles (AuDSNPs) stabilized by a dendrimer, high resistivity nitrogen-doped carbon nano-tubes (CNx) and a high specificity cellular localization oligonucleotide aptamer, and has a nanometer layered structure. The composite nanometer structure electrochemical cell sensor can be provided for carrying out selective quantification detection on expression of DR4 / DR5 death receptors on leukemic cells and surfaces thereof. The present invention further discloses the preparation method.
Owner:NANJING UNIV
Blood leukocyte segmentation method based on adaptive histogram threshold value and contour detection
ActiveCN110120056AImprove Segmentation AccuracyAchieve positioningImage enhancementImage analysisStainingPrior information
The invention relates to a blood leukocyte segmentation method based on an adaptive histogram threshold value and contour detection. The method comprises the following steps: firstly, enhancing the contrast ratio of cell nuclei through color component combination, and performing coarse segmentation on the cell nuclei by utilizing an adaptive histogram threshold technology to realize leukocyte localization; then, sequentially removing an image background and red blood cells by using the color prior information, and then executing edge detection to obtain a maximum connected component as an initial white blood cell contour; and finally, using image filling and morphological operation to realize extraction of a leukocyte region, so that a cytoplasm segmentation result is obtained. Experimental results on standard and rapid staining leukocyte image data sets prove that the method provided by the invention improves the leukocyte segmentation precision under two preparation conditions.
Owner:江苏谷梵智能科技有限公司
Up-conversion luminescent nano-sensing material for detection of arginine and application thereof in detection of the arginine
InactiveCN106947467AReduce harmAvoid autofluorescenceMaterial nanotechnologyOrganic chemistryArginineRare earth
The invention relates to a novel ratio-luminescent up-conversion nano-sensing material, which is used for detecting the lysosome cell localization luminescence resonance energy transfer principle of arginine, and a preparation method of same. The nano-sensing material is prepared through the steps of: with oxides of yttrium, ytterbium and erbium as raw materials to produce a rare earth up-conversion luminescent nano-crystal; modifying the rare earth up-conversion luminescent nano-crystal with polyacrylic acid (PAA) and supporting a 2,3,3-trimethyl-3H-indole-5-carboxylic acid derivative on the polyacrylic acid in the rare earth up-conversion luminescent nano-crystal, excitation wavelength being 980 nm. The material has low damage on cell and effectively avoids autofluorescence of an organism and has good tissue penetrating capability. The material detects the arginine in a ratio luminescence manner; in addition, as a novel ratio-luminescent up-conversion sensor for detecting the lysosome cell localization luminescence resonance energy transfer principle of arginine, the material has good stability and anti-interference property and can be used for detecting the arginine in solutions and living cells.
Owner:ZHENGZHOU UNIV
Automatic cell localization method based on minimized model L1
InactiveCN103236052AAchieve positioningPositioning appliesImage analysisL1 minimizationComputer vision
The invention discloses an automatic cell localization method based on a minimized model L1. The automatic cell localization method includes (1) performing binarization processing to an original image, and acquiring a binarization image B; (2) etching the B, acquiring neuronal connected domains, and performing processing each connected domain according to the steps (3) and (4), and acquiring positions of all cells; (3) embedding each connected domain into a rectangle, namely a sub-region, acquiring candidate seed points from the sub-region, removing redundant candidate seed points according to spaces among the candidate seed points, taking rest candidate seed points as actual seed points, and marking the number of the actual seed points as k; and (4) establishing the minimized model L1, and acquiring actual cell positions and radius in the connected domains. By the aid of the automatic cell localization method, cell localization is performed in a sub-region extracting manner, calculation of each sub-region can be performed simultaneously and independently, all the connected domains can be calculated in a parallel manner, entire calculation speed can be greatly increased, so that wide-range data can be processed effectively.
Owner:HUAZHONG UNIV OF SCI & TECH
Methods for diagnosing osteoarthritis
InactiveUS20100028882A1Efficiently transferPreventing accumulation retentionMicrobiological testing/measurementImmunoglobulins against animals/humansENCODEProhibitin Gene
Methods are disclosed for assessing risk of developing osteoarthritis. Said methods comprise determining the cellular localizations, blood or synovial fluid concentrations, sumoylation states and post-translational modifications of pituitary homeobox transcription factor 1 (pitx-1) repressor proteins, identifying mutations in genes which encode said proteins and identifying any mutations or post-translational modifications causing the nuclear accumulation or retention of said proteins. Said proteins include prohibitin (PHB-I), prohibitone (PHB-2) and B cell lymphoma-6 transcriptional repressor interacting co-repressor (BCoR).
Owner:CHU SAINTE JUSTINE
Methods for Improving Efficiency of Cell Electroporation Using Dielectrophoreses
InactiveUS20090000948A1High efficiency cell electroporationImprove efficiencyElectrostatic separatorsSludge treatmentElectrical field strengthDielectrophoresis
The present invention provides methods for enhancing the efficiency of cell electroporation using dielectrophoresis-assisted cell localization and uses thereof in a microfluidic biochip system. Cells are first subject to dielectrophoresis and localized to regions where the electric field intensity is high enough to render cells electroporated. The invention enhances the efficiency of in situ cell electroporation on a traditional microfluidic biochip.
Owner:CAPITALBIO CORP
Phosphorescent transition metal complex, its preparation and use
ActiveUS20170114275A1Good light fastnessNo undesired self-quenchingBiological testingFluorescence/phosphorescenceDiketoneAryl
A phosphorescent transition metal complex especially suitable as sensor for hydrogen peroxide in cells, in particular in mitochondria in live cells, includes a metallic central atom, which is a transition metal, a first ligand with at least one pyridine ring, and a second ligand having at least one pyridine ring and at least one structural component selected from the group of 1,2-diketone moiety and an arylboronate moiety. A method for preparing the metal complex is also disclosed as well as a method of detecting hydrogen peroxide in cells by incubating cells with the transition metal complex. The metal complex shows advantageous intense and long-lived phosphorescence accompanied by exceptional cellular localization properties. The metal complex is highly photostable and highly selective towards hydrogen peroxide, thus, especially suitable for detecting mitochondrial H2O2.
Owner:CITY UNIVERSITY OF HONG KONG
Zea mays zinc iron-regulated transporter ZmZIPs genes and applications thereof
The invention discloses a Zea mays zinc iron-regulated transporter ZmZIPs genes and applications thereof. Five Zea mays zinc iron-regulated transporter ZmZIP4 and ZmZIP6 genes are separated from Zea mays, and the cDNA sequences of the genes are selected from nucleotide sequences represented by SEQ ID No.1, SEQ ID No.3, SEQ ID No.5, SEQ ID No.7 and SEQ ID No.9 respectively. Subcellular localization shows that a zinc iron-regulated transporter encoded by the genes is localized in the plasma membrane and endoplasmic reticulum of a cell. Yeast complementation experiments show that each of the five ZmZIPs has a zinc and iron transportation function. The separated ZmZIPs genes have important application prospects in the regulation of zinc and iron absorption, transportation and storage of plants, promotion of the embryo and endosperm growth and increase of the content of zinc and iron in grains of food crops.
Owner:HEBEI AGRICULTURAL UNIV.
MYB transcription inhibition factor LrMYB3 related to lycium ruthenicum anthocyanin synthesis and application thereof
The invention discloses an MYB transcription inhibition factor LrMYB3 related to lycium ruthenicum anthocyanin synthesis and application thereof, and belongs to the technical field of gene engineering. According to transcriptome data of lycium ruthenicum, the MYB transcription inhibition factor LrMYB3 participating in anthocyanin synthesis is screened and cloned according to annotation results and expression quantity differences of MYB transcription factors, and belongs to a R2R3 type MYB transcription factor. Multiple sequence alignment and evolutionary tree analysis show that the transcription inhibition factor belongs to a FaMYB1-like transcription inhibition factor. QRT-PCR analysis shows that: the LrMYB3 is expressed in each tissue of the lycium ruthenicum, and the expression level is gradually increased along with ripening of the lycium ruthenicum. Subcellular localization and transcriptional activity detection experiments show that the LrMYB3 is a transcription factor which is localized in a cell nucleus and has no activation function.
Owner:WOLFBERRY SCI INST NINGXIA ACAD OF AGRI & FORESTRY SCI
Construction of live attenuated Shigella vaccine strains that express CFA/I antigens (CfaB and CfaE) and the B subunit of heat-labile enterotoxin (LTB) from enterotoxigenic E. coli
ActiveUS7759106B2Reduce intrusionBacterial antigen ingredientsBacteriaHeterologousMucosal Immune Responses
Owner:UNITED STATES OF AMERICA THE AS REPRESENTED BY THE SEC OF THE ARMY
Method for detecting location of resveratrol in target cells
The invention discloses a method for detecting the location of resveratrol in target cells. Specifically, the method comprises the following steps of: firstly pretreating the target cells with resveratrol based on the autofluorescence property of resveratrol; then treating the target cells by using a specifically fluorescently-labeled subcellular organelle probe; and finally determining the distribution of resveratrol in the subcellular organelle of the target cells according to the overlap state of the autofluorescence of resveratrol and the fluorescence of the subcellular organelle probe in the cells. The method disclosed by the invention can be used for detecting the distribution of resveratrol in target cells, and has wide application prospect.
Owner:ARMY MEDICAL UNIV
PP1 Ligands
The invention relates to phosphatase ligands and polyligands. In particular, the invention relates to ligands, homopolyligands, and heteropolyligands that modulate PP1 activity. The ligands and polyligands are utilized as research tools or as therapeutics. The invention includes linkage of the ligands, homopolyligands, and heteropolyligands to a cellular localization signal, epitope tag and / or a reporter. The invention also includes polynucleotides encoding the ligands and polyligands.
Owner:PRECIGEN INC
Method for accurately distinguishing cell cycle
The invention relates to a method for accurately distinguishing cell cycle. According to change rules of DNA and RNA in cell nucleus in cell cycle and the characteristic of protein phosphorylation in cell nucleus in mitotic phase, through cell localization by multiple parameters and by fluorescence detection with a flow cytometer, the cell cycle is effectively and accurately classified into five groups, namely G0, G1, S, G2 and M. The invention discloses, for the first time, a method for accurately distinguishing cell cycle. The method is simple and easy to operate. By the method, the defect that cell cycle cannot be distinguished accurately by a present method is solved. Results of the method provided by the invention are stable and reliable, and the method is suitable for detection of various cells.
Owner:SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
23-hydroxybetulinic acid fluorescent probe and its preparation method and use in cellular localization and uptake
InactiveCN105541955AGood effectAntitumor effectOrganic active ingredientsSteroidsDrugOrganic synthesis
The invention relates to the field of novel 23-hydroxybetulinic acid fluorescent derivative organic synthesis and pharmaceutical chemistry and concretely relates to a 23-hydroxybetulinic acid fluorescent probe obtained through connection of a coumarin fluorophore to a 23-hydroxybetulinic acid skeleton. The invention discloses a use of the 23-hydroxybetulinic acid fluorescent derivative in antineoplastic mechanism research and a potential use of the 23-hydroxybetulinic acid fluorescent derivative in cancer treatment and especially relates to a use of the 23-hydroxybetulinic acid fluorescent probe in detection of target cell localization and uptake under action of 23-hydroxybetulinic acid.
Owner:CHINA PHARM UNIV
Recombinant Live Attenuated Foot-and-Mouth Disease (FMD) Vaccine Containing Mutations in the L Protein Coding Region
ActiveUS20110177123A1More attenuationReduce probabilitySsRNA viruses positive-senseViral antigen ingredientsSubcellular distributionWild type virus
Previously we have identified a conserved domain (SAP, for SAF-A / B, Acinus, and PIAS) in the foot-and-mouth disease virus (FMDV) leader (L) protein coding region that is required for proper sub-cellular localization and function. Mutation of isoleucine 55 and leucine 58 to alanine (I55A, L58A) within the SAP domain resulted in a viable virus that displayed a mild attenuated phenotype in cell culture, along with altered sub-cellular distribution of L and failure to induce degradation of the transcription factor nuclear factor kappa-B. Here we report that inoculation of swine and cattle with this mutant virus results in the absence of clinical disease, the induction of a significant FMDV-specific neutralizing antibody response, and protection against subsequent homologous virus challenge. Remarkably, swine vaccinated with SAP mutant virus are protected against wild type virus challenge as early as two days post-vaccination suggesting that a strong innate as well as adaptive immunity is elicited. This variant could serve as the basis for construction of a live-attenuated FMD vaccine candidate.
Owner:US SEC AGRI
Immunohistochemical nuclear staining section cell positioning multi-domain co-adaptation training method
The invention relates to an immunohistochemical nuclear staining section cell positioning multi-domain co-adaptation training method which is used for fully training a cell key point detection model under an only single-domain labeling data set. The method includes training the cell positioning model by adopting the source domain image and the target domain image, alternately inputting the sourcedomain image and the target domain image into an encoder to perform feature extraction, performing feature extraction on the source domain image to obtain a first feature, and performing feature extraction on the target domain image to obtain a second feature; inputting the first feature and the second feature into a discriminator for feature discrimination; when the loss function of the discriminator reaches a set condition, taking the extracted first feature and second feature as domain invariant features; alternately inputting the first feature and the second feature into a decoder for decoding, and performing activation operation to obtain a corresponding confidence map; in the training process, enabling the encoder and the decoder to perform parameter updating through continuous iteration; and when the number of training iterations reaches a specified number, ending the training.
Owner:杭州迪英加科技有限公司
Compositions and methods for efficient targeting of transgenes
ActiveUS10563220B2Effective controlNot to damagePolypeptide with localisation/targeting motifOther foreign material introduction processesBiotechnologyTransgene
The invention provides recombinant DNA molecules and constructs useful for providing efficient transgene sub-cellular localization of proteins in transgenic plants. Recombinant DNA molecules and constructs for conferring herbicide tolerance or resistance to plants are further provided, as well as plants exhibiting herbicide tolerance and methods for producing or utilizing such plants.
Owner:MONSANTO TECH LLC
Multifunctional plant expression vector as well as construction method and application thereof
PendingCN111187787AIncrease brightnessEasy to observe positioning resultsVector-based foreign material introductionBiotechnologyNucleotide
The invention discloses a multifunctional plant expression vector as well as a construction method and application thereof, and belongs to the technical field of genetic engineering. The multifunctional plant expression vector is Pesnoo-eYGFPur-apply, and the nucleotide sequence is shown in SEQ ID NO.1. Due to the addition of upstream and downstream regulatory elements of a target gene, the expression of the target gene can be significantly enhanced compared to a common overexpression vector driven by a CaMV 35S promoter, and at the same time, due to the fusion expression of the target gene and eYGFPuv, the expression of the eYGFPuv is also significantly enhanced; during subcellular localization observation, compared with an ordinary subcellular localization vector, the target gene and eYGFPuv fusion protein in cells for transforming the vector has higher brightness, so that the localization result is more conveniently observed; and the vector is used for plant genetic transformation,when a positive test is performed, people only needs a UV flashlight to irradiate a tissue sample or a plant sample to judge whether the sample is positive by observing the presence or absence of excited green fluorescence, so that the workload of molecular testing of transgenic samples is greatly reduced.
Owner:INST OF COTTON RES CHINESE ACAD OF AGRI SCI
Screening method for genes related to physical ability of excellent cross-country skiers
The invention provides a screening method for the genes related to physical ability of excellent cross-country skiers. The screening method provided by the invention comprises the following steps of: taking an excellent cross-country skier system as an object; comparing the physical ability differential gene expression profiles of the excellent cross-country skiers by applying a high-flux oligonucleotide chip technology; analysing and explaining the molecular functions, signal pathways and cellular localization information of differential genes via gene ontology, thus screening the target pathways related to physical ability heredity; looking for the pathways where the differential genes are located via BioCarta and KEGG website; screening the significant target pathways by applying a hypergeometric distribution statistical method; and verifying the differential genes by real-time fluorescence quantification (RT-PCT), so as to obtain the target genes related to physical ability of the excellent cross-country skiers.
Owner:HARBIN INST OF PHYSICAL EDUCATION
Integrated micro-fluidic neural network chip and bionic implementation method
ActiveCN108537333ACirculation route reservationIsolated from circulationPhysical realisationControl layerNerve cells
The invention discloses an integrated micro-fluidic neural network chip and a bionic implementation method. A flow layer of the chip is provided with a micro-cavity array, a connecting pipeline network, two groups of transmission pipeline networks and an inlet / outlet; the two groups of transmission pipeline networks are respectively positioned at the upper and lower ends of the micro-cavity arrayand communicated with the micro-cavity array; a control layer comprises a transmission valve, a connecting valve, an air inlet pipeline and an air inlet; the connecting valve is arranged just under the connecting pipeline between two adjacent groups of micro-cavities; the transmission valves corresponding to the two inlet / outlet transmission pipelines are respectively positioned on the outlet sideand the inlet side; between two optional groups of micro-cavities, at least one transmission valve exists in the transmission valves on each micro-cavity transmission pipeline communicated with any one group of micro-cavities, and all the transmission valves on the micro-cavity transmission pipeline of the other group of micro-cavities are not communicated with the same air inlet. Based on the chip of the structure, functions of arrayed cell localization and inoculation, bionic neural network building, biochemical stimulus treatment of nerve cells and neural network in a single group of micro-cavity areas and sample recovery can be realized.
Owner:CENT SOUTH UNIV
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