83 results about "Cell localization" patented technology

An imaging fountain flow cytometer allows high resolution microscopic imaging of a flowing sample in real time. Cells of interest are in a vertical stream of liquid flowing toward one or more illuminating elements at wavelengths which illuminate fluorescent dyes and cause the cells to fluoresce. A detector detects the fluorescence emission each time a marked cell passes through the focal plane of the detector. A bi-directional syringe pump allows the user to reverse the flow and locate the detected cell in the field of view. The flow cell is mounted on a computer controlled x-y stage, so the user can center a portion of the image on which to zoom or increase magnification. Several computer selectable parfocal objective lenses allow the user to image the entire field of view and then zoom in on the detected cell at substantially higher resolution. The magnified cell is then imaged at the various wavelengths.

With the goal of creating a combination vaccine against Shigella and other diarrheal pathogens we have constructed a prototype vaccine strain of Shigella flexneri 2a (SC608) that can serve as a vector for the expression and delivery of heterologous antigens to the mucosal immune system. SC608 is an asd derivative of SC602, a well-characterized vaccine strain, which has recently undergone several phase 1 and 2 trials for safety and immunogenicity. Using non-antibiotic asd-based plasmids, we have created novel constructs for the expression of antigens from enterotoxigenic E. coli (ETEC), including CFA / I (CfaB and CfaE) and the B-subunit from heat-labile enterotoxin (LTB) in Shigella vaccine strain SC608. Heterologous protein expression levels and cellular localization are critical to immune recognition and have been verified by immunoblot analysis. Following intranasal immunization (SC608(CFAI) and SC608(CFAI / LTB) of guinea pigs, serum IgG and IgA immune responses to both the Shigella LPS and ETEC antigens can be detected by ELISA. In addition, ELISPOT analysis for ASCs from cervical lymph nodes and spleen showed similar responses. All vaccine strains conferred high levels of protection against challenge with wild-type S. flexneri 2a using the Sereny test. Furthermore, serum from guinea pigs immunized with SC608 expressing CfaB and LTB contained antibodies capable of neutralizing the cytological affects of heat-labile toxin (HLT) on Chinese Hamster Ovary (CHO) cells. These initial experiments demonstrate the validity of a multivalent invasive Shigella strain that can serve as a vector for the delivery of pathogen-derived antigens.

The invention provides recombinant DNA molecules and constructs useful for providing efficient transgene sub-cellular localization of proteins in transgenic plants. Recombinant DNA molecules and constructs for conferring herbicide tolerance or resistance to plants are further provided, as well as plants exhibiting herbicide tolerance and methods for producing or utilizing such plants.