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Integrated microfluidic chip and application thereof in living cell control and analysis

A microfluidic chip and cell technology, which is used in tissue cell/virus culture devices, microorganism measurement/inspection, biochemical instruments, etc., to achieve good biocompatibility and excellent light transmittance.

Inactive Publication Date: 2009-12-09
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Regarding the microfluidic chip for cell interaction, only a simple parallel single-channel level has been realized [A.P.Wong, R.Perez-Castillejos, L.J.Christopher, et al., Biomaterials 2008, 29(12): 1853-1861. 】

Method used

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  • Integrated microfluidic chip and application thereof in living cell control and analysis
  • Integrated microfluidic chip and application thereof in living cell control and analysis
  • Integrated microfluidic chip and application thereof in living cell control and analysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Such as figure 1 As shown, the integrated microfluidic chip prepared in this example is composed of three layers: a flow layer, a control layer and a cover layer (from top to bottom). Among them, there are eight sample inlets (such as figure 1 and 2, each with 4) and two sample outlets (such as figure 3 and 4) around the fluidized layer, and there are four circular cells in the middle. For the culture chambers (such as figure numbers 9, 10, 11, 12), the adjacent chambers are connected two by two by thin pipes 13 for cell interaction research.

[0025] The control layer is provided with a total of 28 air inlets (such as number 5, 6, 7, and 8, each with 7), which are respectively connected to the closed microvalve switch at the end of a single pipeline.

[0026] Production of microfluidic chips (taking the production of PDMS microfluidic chips as an example):

[0027] 1. Using soft lithography technology to prepare the chip mold, first make the design structure of the f...

Embodiment 2

[0030] The control of the microvalve switch on the air inlet of the control layer to the flow layer pipeline is as follows: figure 2 As shown, when the air inlet 24 does not take in air, the microvalve switch pipeline 23 is in the state of atmospheric pressure, and the microvalve switch 22 is in a closed state. At this time, the fluidized layer pipeline 21 is unblocked, and the samples in the pipeline can enter and exit at will; when the lower pressure (20psi) when the gas 32 enters the microvalve switch pipeline ( image 3 As shown), the microvalve switch 31 expands and presents a protrusion, so that the fluidized layer pipeline is in a half-closed state; further, as Figure 4 As shown, when the higher pressure (40psi) gas 42 enters the microvalve switch pipeline, the microvalve switch 41 is completely close to the fluidized layer pipeline, sealing the fluidized layer pipeline, thereby achieving the purpose of controlling the entry and exit of samples in the fluidized layer ...

Embodiment 3

[0032] This embodiment takes the localized culture of liver cancer cells and fibroblasts in a microfluidic chip as an example.

[0033] 1. First, the microfluidic chip prepared in Example 1 is autoclaved, and then the fluidized layer pipeline and cavity are cleaned (use a micro-syringe pump to inject samples, and the cleaning solution is 0.01M PBS, pH=7.4, flow rate 25μL / min, time 15min), and then coat and modify the surface of the flow layer pipe and cavity (coating modifier is poly-L-lysine, 100μg / mL, 37°C, 2h), and then wash (Sampling is also injected using a micro-syringe pump, 0.01M PBS is selected as the cleaning solution, pH=7.4, the flow rate is 25 μL / min, and the time is 15 min), blow dry with sterile gas, and set aside.

[0034] 2. Preparation of liver cancer cells and fibroblast suspensions. In this example, liver cancer cell line cells (HepG2 cells) and fibroblast cell line cells (NIH 3T3 cells) were selected, and the number of cells was amplified by conventional i...

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Abstract

The invention discloses an integrated microfluidic chip applied to localization, culture and interaction of different cells. The chip is composed of a flow layer, a control layer and a covering layer from up down; wherein, the flow layer is mainly composed of a micron pipe network and four round cavities, every two adjacent cavities are connected by a small pipe, and the flow layer is provided with ten sample inlet / outlets in total; the control layer is composed of a plurality of enclosed micro valve switches at the terminal of single pipe and can control time and space of input and output of samples at different pipe positions of the flow layer. The integrated microfluidic chip of the invention has the advantages of good quality of preparation material, strong repeatability of preparation method and wide application range and can be used for research on localization co-culture and interaction of multiple cells.

Description

technical field [0001] The invention relates to a microfluidic chip, in particular to an integrated microfluidic chip. The integrated microfluidic chip can be used to conduct time and space controlled positioning culture and interaction research of different types of cells. Background technique [0002] Microfluidic chip technology refers to the integration of basic operating units such as sample preparation, reaction, separation and detection involved in the fields of biology and chemistry into a chip of a few square centimeters or smaller, and the fluid is transported through the microchannel network. A technology that controls the biological or chemical reaction process and analyzes the reaction products of the whole system. The biggest advantage of microfluidic chips lies in the flexible combination and large-scale integration of various functional unit technologies in tiny sizes, resulting in maximum automation, high throughput and low consumption. At the same time, th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12Q1/02
Inventor 王进义刘文明李立宋鹏任丽王雪琴任莉莉王旭明
Owner NORTHWEST A & F UNIV
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