38 results about "Nuclear staining" patented technology

The invention provides a single cell separation method based on droplet micro-fluidic chips. The method concretely comprises the following steps: A, a single cell suspension flows into a dispersed phase inlet channel from a dispersed phase inlet; B, an oil phase liquid flows into a continuous phase inlet channel from a continuous phase inlet; C, the above two phases merge to form droplets enclosing single cells, the droplets flow over a droplet capturing unit with the generation of a large number of droplets in a liquid storage pool, and stand for 2-5 min, and the superfluous droplets are sucked out when the droplets slowly settle into the droplet capture unit; and D, the droplet chips which capture the single cells are cultured in a 37 DEG C incubator, then DAPI is added to carry out nuclear staining, and the single cell capture rate is detected. The method has the advantages of simplicity and rapidity in operation, small use amounts of the cells and reagents, low experiment cost, high integration and wide application range.

The invention discloses a breast cancer Ki67/ER/PR nuclear staining cell counting method based on staining separation. The method comprises the steps that firstly, a breast cancer pathological sectionimage is dyed and separated, an image IH based on an H coloring agent and an image IDAB based on a DAB color developing agent are obtained, then the influence of intercellular cytoplasm is eliminatedthrough filtering, and an image IH, filterd, IDAB and filterd are obtained; secondly, counting the negative cells according to IH and filterd, counting strong positive, middle positive and weak positive cells according to IDAB, filterd and the brightness map VDAB of the IH and filterd, and finally obtaining the number of the negative cells, the number of the positive cells and the number of the positive cells (divided into strong positive, middle positive and weak positive) and the probability of occupying the total number of the cells. According to the breast cancer nuclear staining cell counting method based on staining separation, cell counting can be rapidly and effectively achieved, doctors are assisted in work, the workload of the doctors is reduced, and meanwhile the calculation accuracy is guaranteed.

The present invention relates to compositions, methods, and kits suitable for detecting nucleic acids in a biological sample. The nuclear staining composition of the present invention contains a pH buffering reagent, a solubilizing reagent, a basic dye, and an aqueous medium. The composition can be used alone to detect nucleic acids in a biological sample or in combination with other histological dyes for nuclear counterstaining.

The invention relates to a full-automatic liquid-based cell tableting and staining integrated machine, comprising: a mounting frame, a circulating sample injection mechanism, a specimen liquid puncture-adding mechanism, a slide loading mechanism, a code printing mechanism, an alcohol fixing and nuclear staining mechanism, a cytoplasm staining mechanism, a rinsing mechanism, a slide collecting mechanism and a slide clamp circulating mechanism, wherein the mounting frame is the skeleton of the whole machine; the slide clamp circulating mechanism is used for circulating a slide clamp in the machine; the circulating sample injection mechanism is used for transmitting a sample to the specimen liquid puncture-adding mechanism; the specimen liquid puncture-adding mechanism is used for adding thesample liquid to the slide clamp; the slide loading mechanism is used for loading a slide into the slide clamp; the code printing mechanism is used for printing a code on the slide in the slide clamp;the alcohol fixing and nuclear staining mechanism is used for carrying out fixing and nuclear staining on the sample; the cytoplasm staining mechanism is used for carrying out cytoplasm staining on the sample; the rinsing mechanism is used for washing floating color of the sample; and the slide collecting mechanism is used for taking out the slide from the slide clamp and putting into a collection box. The machine is easy to operate, high in automation degree and high in efficiency.

A method is provided for improving the shelf-life of a nuclear staining solution. In particular, the present invention provides a method of adding an antioxidant to a hematoxylin staining solution which maintains the performance of the stain over its shelf-life.

The invention provides a fluorescent carbon dot for nuclear staining. The fluorescent carbon dot is prepared from the following steps: 1) dissolving folic acid and m-phenylenediamine in water, and carrying out heating for a reaction; and 2) after the reaction, performing centrifuging and silica gel column chromatography, collecting a yellow-green fluorescent part, transferring the yellow-green fluorescent part into water after rotary evaporation and then carrying out lyophilization. The carbon dot prepared in the invention emits green light under ultraviolet excitation, has large Stokes shift,is consistent with the existing commercial 405 laser at the optimal excitation position, and can be well applied to a laser confocal microscope.

The invention provides a method for diagnosing a melanocytic proliferation in a subject comprising staining a sample of lesional melanocytes with an antibody against soluble adenylyl cyclase (sAC) and interpreting the sAC staining pattern, which is associated with a diagnosis of a melanocytic proliferation. The sAC staining pattern, which is complex, is discriminatory and distinctive according to the nature of the melanocytic proliferation. The sAC staining pattern comprises one or more of dot-like Golgi staining, broad granular Golgi staining, diffuse cytoplasmic staining, nucleolar staining, incomplete granular nuclear staining, and pan-nuclear staining. The method of the invention is particularly useful in confirming or disaffirming a diagnosis reached through conventional histologic examination of a sample. Additionally, the invention provides a kit for use in interpreting melanocytic proliferations.

The invention relates to a nuclear staining cell counting method based on deep learning of an incomplete label, computer equipment and a storage medium, and the method comprises the following steps: (1) making label data: loading a pathological image to label software, and obtaining sub-mask image data pairs of all sub-images and positive cells; (2) model training: training a convolutional neural network model to respectively obtain a trained positive cell convolutional neural network model and a trained negative cell convolutional neural network model; (3) reasoning stage: inputting pathological images to be detected into the trained convolutional neural network model to obtain real mask images; and (4) post-treatment stage: calculating the number of positive cells and negative cells, and calculating to obtain the proportion p of the positive cells in all the cells. The method does not need extra parameters, is high in universality, greatly reduces manual adjustment, and effectively improves the recognition accuracy and robustness. The method is faster, more accurate and more effective in data marking.

The invention discloses a Pap staining solution and application thereof, and belongs to the field of biotechnology. Specifically, the Pap staining solution contains a cell nuclear staining solution and a cytosolic staining solution. The Pap staining solution provided by the invention is simple to operate, and ensures uniform staining, clear cell structure after staining and bright cell colors, soas to facilitate diagnostic observation and judgment of clinical pathology.

The invention belongs to the technical field of image analysis, and discloses a DNA fluorescence in situ hybridization BCR/ABL fusion state detection method and a detection system, which are characterized in that a CNN identification model is utilized to generate pseudonucleus staining of cells from a phase difference image, and FISH cell nucleuses are positioned and classified; and positioning and classifying each independent fluorescence signal, and dividing the number of color development points of the same classification in each photo by the total number of display points in the photo to obtain a BCR/ABL fusion ratio. The invention provides a system for detecting the fusion level of cell nucleuses and BCR/ABL genes by analyzing fluorescence in situ hybridization (FISH) images and calculating the image ratio of the number of abnormal cell nucleuses related to all classified cell nucleuses as an index for classifying the BCR/ABL gene fusion states of corresponding tumor samples. The detection method is high in detection efficiency, accurate in detection result and capable of achieving automatic detection.

The invention relates to an immunohistochemical nuclear staining section cell positioning multi-domain co-adaptation training method which is used for fully training a cell key point detection model under an only single-domain labeling data set. The method includes training the cell positioning model by adopting the source domain image and the target domain image, alternately inputting the sourcedomain image and the target domain image into an encoder to perform feature extraction, performing feature extraction on the source domain image to obtain a first feature, and performing feature extraction on the target domain image to obtain a second feature; inputting the first feature and the second feature into a discriminator for feature discrimination; when the loss function of the discriminator reaches a set condition, taking the extracted first feature and second feature as domain invariant features; alternately inputting the first feature and the second feature into a decoder for decoding, and performing activation operation to obtain a corresponding confidence map; in the training process, enabling the encoder and the decoder to perform parameter updating through continuous iteration; and when the number of training iterations reaches a specified number, ending the training.

The invention discloses a cell nucleus DNA staining method. The method comprises the following steps: preparing an AF stationary liquid, a hydrolysate, a rinsing liquid and an eosin staining liquid; preparing a cell DNA staining solution which is composed of a cell DNA staining solution A and a cell DNA staining solution B in a volume ratio of 1: 1; placing a pathological sample into the AF stationary liquid to be fixed; washing the sample with running water, and then placing the sample into a hydrolysate to hydrolyze; washing the sample with running water, and then placing the sample in the cell DNA staining solution for staining; washing the sample with running water, and then placing the sample in a rinsing liquid for rinsing; and carrying out dehydrating with ethanol step by step, placing the sample into an eosin staining solution, and carrying out decolorizing with absolute ethyl alcohol, airing, and sealing. According to the method, dyeing time is greatly shortened, the hydrolysate is composed of non-precursor chemicals and belongs to an environment-friendly reagent, compared with a traditional stationary liquid, the AF stationary liquid does not include acetic acid and lesssimulates the human body, and the cell DNA staining solution is simple to prepare, does not need to be heated and boiled, has the validity period longer than 18 months, is ready-to-use and is suitablefor commercial popularization.

The invention discloses a method for nanotube-height-aided control of cytoskeleton change. The method comprises steps of performing anodization of pure titanium or low-modulus biphase titanium using an organic solvent; representing the microscopic structure of an anodization product using a microscopic means; preparing a titanium metal piece of nanopore and naotube microstructures; separating bone narrow mesenchymal stem cells, adhering three-generation cells on a surface-modified metal piece for incubation; observing cell adhesion effects by MTT, DYPI nuclear staining and SEM; placing the metal testing piece for cell incubation in culture holes of an aseptic elastic plate, and placing the metal testing piece in a universal testing system in a constant temperature state and providing the metal testing piece with different periods, frequencies and load compressive stress; and observing and testing cell deformation with a SEM electron microscope or optical microscope, and detecting representation of cell proliferation and differentiation. The method enables the elastic deformation signal of a modified titanium substrate surface to be amplified, thereby influencing osteoblast adhered on the modified titanium substrate surface to receive greater simulation, and leading to larger cytoskeleton change.

The invention discloses a spinal cord needle biopsy tissue fixation decalcification solution, a preparation method and a decalcification method. The spinal cord needle biopsy tissue fixation decalcification solution is prepared from the following components in parts by weight: 30-35 parts of formaldehyde, 300-400 parts of methanol, 400-500 parts of an ethylenediamine tetraacetic acid saturated solution, 7-9 parts of a PBS buffer solution, 25-35 parts of an accelerant, 1-3 parts of sodium chloride and 100-150 parts of water. The decalcification solution is small in tissue damage and better in tissue integrity, meanwhile, the decalcification time is greatly shortened, the decalcification time can be shortened from traditional 72 h to 24 h, the requirement for rapidly obtaining a pathological diagnosis report is greatly met, meanwhile, excimer detection can be conducted, accurate medication can be conducted on later-stage targeted therapy, the diagnosis efficiency is improved, and after decalcification treatment, the staining effect of a tissue specimen is better, the structure is clearer, the structures of soft tissues and bone groups in the section are completely displayed, the cell nucleus staining is clear, the nucleoplasm contrast is distinct, and the method has higher application value in the spinal cord needle biopsy tissue sheet preparation.

This invention provides isolated nucleic acid and amino acid sequences encoding VG5Q, a novel angiogenic growth factor protein with pro-angiogenic activity, a forkhead-associated domain, a G-patch domain; characteristic subcellular localization in an in vitro Matrigel model of angiogenesis: towards the cell periphery in early stages of tubulogenesis, between cells in newly formed endothelial tubes, and no nuclear staining after 24 hours; is expressed in endothelial cells; is secreted during angiogenesis; and interacts with TWEAK. The invention also provides for expression vectors containing nucleic acid sequences encoding VG5Q protein, and host cells containing one or more expression vectors for the recombinant expression of VG5Q. The invention also provides for methods of using VG5Q for the diagnosis and treatment of angiogenesis-mediated diseases or disorders.

The invention relates to a method for detecting morchella mycelium nucleus concentration through nucleus staining. The method comprises the following steps: carrying out mycelium culture by using a sterile plate containing a PDA culture medium; taking out cover glass covered with morchella mycelium from a culture medium, and dividing the cover glass into a control group and an experimental group;soaking the cover glass of the control group and the experimental group in a solvent, washing the cover glass after soaking is finished, and dropwise adding a fixing solution to the cover glass afterwashing is finished; washing the morchella mycelia fixed by the fixing solution by using a buffer solution, putting the cover glass of the control group and the experimental group into a clean dyeingbox, and adding a dyeing fixing solution into the dyeing box; washing the dyed morchella mycelia by using a buffer solution, sucking the residual buffer solution on the cover glass by using filter paper, and dropwise adding an anti-fluorescence quenching slide sealing solution into the center of the cover glass, so that the morchella mycelia are in contact with the anti-fluorescence quenching slide sealing solution to prepare slide samples of the control group and the experimental group. Understanding of factors such as growth, development, propagation and senescence of morchella esculenta andgrowth vigor and yield in the cultivation process is facilitated, and prejudgment is facilitated.