8 results about "Nuclear staining" patented technology

This invention proposes a sperm staining kit based on a sequential staining strategy of tail pre-staining – rapid differentiation – enhanced nuclear staining. A gentle fixation with 4% paraformaldehyde and acetate buffer preserves the complete nucleic acid of the sperm head and the protein structure of the tail. The addition of PVP K30 and Tween-20 effectively inhibits non-specific adsorption and reduces staining background interference. Brilliant Green SF combined with sodium tartrate is used for specific staining of the sperm tail, followed by rapid differentiation to wash away non-specific staining. Nuclear staining is performed using toluidine blue O combined with sodium chloride and sodium tetraborate hydrate, specifically binding to DNA and enhancing color development stability, resulting in a blue sperm head and a light green tail with clear color contrast. This invention produces a clean staining background, accurately distinguishing the morphology of the sperm head, acrosome, and tail, significantly improving the accuracy of microscopic identification of abnormal sperm such as large-headed, small-headed, curled-tailed, broken-tailed, and short-tailed sperm. It is suitable for detecting sperm morphology and abnormality rates in breeding males.

The invention relates to the technical field of immunohistochemical staining image quantitative analysis, in particular to an immunohistochemical staining image quantitative analysis method and system, and the method comprises the following steps: S1, obtaining a digital image of an immunohistochemical staining section; and S2, carrying out color deconvolution processing on the digital image, and decomposing the digital image into an exclusive channel image corresponding to at least one target coloring agent and a background channel image. According to the method, color deconvolution is realized through a non-negative matrix factorization algorithm, target coloring agent signals and background and other dyeing signals in the composite dyeing image are accurately split, and the problem that dyeing components of an existing system are not thoroughly separated is solved; through combination of a Mask R-CNN deep learning network and multi-mode adaptive parameters, positive and negative cells in different dyeing modes can be accurately identified, and multiple scenes such as nuclear dyeing, membrane dyeing and slurry dyeing can be adapted.

The invention discloses a microscope image analysis method and device based on immunohistochemistry and a medium, belongs to the technical field of medical image analysis, and aims to solve the problems that manual interpretation burden of microscope images under existing immunohistochemistry detection is heavy, the problems of man-made subjectivity and consistency are prominent, the stability is easily influenced by illumination and dyeing differences, and the detection efficiency is low. And the intelligent picture analysis cost is high. The method comprises the following steps: carrying out standardization processing on an original collection image collected by a microscope in real-time visual field to obtain a standardized collection image; pixel segmentation and recording under a cell level are carried out on the standardized collected image; carrying out intensity grade discrimination processing of related nuclear staining markers on the cell-level pixel information to generate a cell-level structured quantification result; performing membrane-related phenotype prediction calculation under a related membrane staining marker on the cell-level pixel information to obtain a cell-level prediction result; and carrying out integration processing on the cell-level structured quantification result and the cell-level prediction result to obtain a cell-level analysis result.

The invention discloses a method for observing the shape and number of synapses in a dentate gyrus region based on image analysis, and relates to the technical field of neurobiology. The method comprises the following steps: S1, tissue treatment: after an experimental animal is anesthetized, pouring a buffer solution and a stationary solution into the heart in stages, separating hippocampus tissue, trimming and fixing, pre-cooling, slicing and preserving at low temperature; s2, dyeing and marking: performing targeted marking on the dentate gyrus area of the sea horse by adopting DiI particles through a fine tungsten steel through needle, and preserving at low temperature after incubation; s3, fixing and mounting: fixing for the second time, and mounting with an anti-fluorescence quenching agent after DAPI nuclear dyeing; s4, confocal imaging is carried out, exclusive parameter layer scanning is set, and qualified dendritic spine images are screened; and image quantitative analysis can be further carried out, and accurate quantification is realized according to classification standards. The method is standard in operation, greatly improves sample quality and experimental result credibility, optimizes experimental efficiency and controllability and reduces technical threshold by means of targeted marking and standardized processes, and is suitable for relevant basis and application research of the hippocampal synapses.

The present inventions provide improved solution compositions and methods for detecting cancerous and dysplastic lesions in mucosal tissue in vivo. Specifically, the disclosed pretreatment solution results in enhanced removal of mucous and tissue debris. The disclosed detection solution compositions (nuclear stain-based and lectin-based) provide enhanced disease detection, when used individually or sequentially, tissue permeation, fluorescent signal, and shelf stability. Lectin fragmentation methods and processes of manufacturing are also disclosed. Additionally, various methods utilizes the disclosed inventive solution compositions are disclosed for accurate disease detection in vivo yielding high sensitivity and specificity values.

The present invention relates to the technical field of spatial transcriptomics, and in particular to a method for identifying a cell boundary applied in spatial transcriptomic analysis. In general, during spatial transcriptomic analysis, the procedure substantially comprises sample preparation, optical structure image generation, spatial transcriptomic information collection, alignment of optical structure images with spatial transcriptomic information, and single-cell annotation. The characteristic of the present invention lies in selecting a specific fluorescent protein transgenic animal model and employing an optical and transcriptional image alignment method compatible with the animal model, thereby enabling clear visualization of the structure and contour of a single cell in a sample under a fluorescence imaging system without requiring cell membrane or nuclear staining to obtain a fluorescence image. Consequently, drawbacks of dye‑induced effects on mRNA capture efficiency and sample displacement are avoided. In addition to significantly improving the efficiency of cell boundary identification, the method further enhances the accuracy of subsequent algorithm‑assisted cell image segmentation and large‑language‑model‑assisted cell annotation.

The invention relates to the technical field of microbiological detection and separation, and provides an intestinal active bacterium screening method based on metabolic activity labeling and fluorescence sorting, which comprises the following steps: firstly, treating an excrement sample in an anaerobic environment, and preparing a pure bacterium suspension through the steps of homogenization, centrifugation, filtration and the like. Then, L-homoalkynyl glycine is used for conducting metabolic labeling on the bacterial liquid, so that the marker is integrated into the body when the bacteria with the activity synthesize new protein. Then fixing the cells, and efficiently and specifically coupling a fluorescent dye to a marker through a click chemical reaction, so as to realize fluorescent labeling of the active bacteria. And finally, carrying out nucleus staining on all cells, carrying out microscope confirmation, and carrying out fluorescence sorting and collection on the target active bacteria according to a fluorescence signal by utilizing flow cytometry.

The invention relates to the technical field of biology, in particular to an economical collagenous fiber dyeing kit and a preparation method thereof, and the economical collagenous fiber dyeing kit comprises the following reagents: modified iron hematoxylin, a Cole hematoxylin solution, a sirius red dyeing solution, a modified eosin dyeing solution, a modified fuchsin solution, a differentiation concentrated solution, an acidic differentiation solution, a bluing solution and a Scott bluing promoting solution. According to the invention, the modified iron-hematoxylin a liquid can enhance the solubility of the dye, delay oxidative browning and prevent precipitation, so that the nuclear dyeing is clearer and more uniform, and the modified iron-hematoxylin b liquid can stabilize the release of iron ions, reduce the excessive corrosion of hydrochloric acid and enhance the dyeing contrast; the mixing of the two ensures that cell nucleus staining has excellent selectivity, distinct contrast ratio and good reproducibility; the modified eosin dye liquor can maintain the pH stability of the dye liquor and enhance the permeability and affinity to cytoplasm; the modified fuchsin solution can rapidly form a uniform and stable working solution, and the dyeing effects of high specificity and distinct contrast on collagenous fibers are achieved.