Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Fluorescent carbon dot for nuclear staining, and application and application method of same to nuclear imaging

A fluorescent carbon dot and fluorescent imaging technology, applied in the field of nanomaterials and biological imaging, can solve the problems of long incubation time and difficult cell nucleus imaging, and achieve the effects of short incubation time, cheap devices and reagents, and low incubation concentration

Active Publication Date: 2018-12-18
ZHENGZHOU UNIV
View PDF3 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the carbon dots invented at present are used for cell imaging, due to the size of the material, surface charge and other properties, it is difficult to apply to the imaging of the nucleus. Most of the invented carbon dots can only enter the cytoplasm, mitochondria and other parts of the cell, and require longer incubation time

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fluorescent carbon dot for nuclear staining, and application and application method of same to nuclear imaging
  • Fluorescent carbon dot for nuclear staining, and application and application method of same to nuclear imaging
  • Fluorescent carbon dot for nuclear staining, and application and application method of same to nuclear imaging

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A fluorescent carbon dot for nuclear staining, the carbon dot is prepared by the following steps:

[0030] 1) Dissolve 5 mg of folic acid and 13 mg of m-phenylenediamine in 5000 mg of deionized water, place in a hydrothermal reactor and heat to 200°C for 12 hours; in other examples, the mass ratio of folic acid and m-phenylenediamine is 1 : (2-20) can be used, the amount of deionized water can be enough to dissolve the raw materials;

[0031] 2) After the reaction, centrifuge (remove large particle aggregates) and take the upper layer solution through silica gel column chromatography, collect the yellow-green fluorescent part, spin evaporate (remove organic solvent), transfer to distilled water, freeze-dry to obtain fluorescent carbon dots; The mobile phase used in silica gel column chromatography is a mixture of methanol and ethyl acetate at a volume ratio of 1:1; the specific operation of lyophilization is: first freeze at -16°C for 30h, and then freeze at -60°C for 4...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
The average particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention provides a fluorescent carbon dot for nuclear staining. The fluorescent carbon dot is prepared from the following steps: 1) dissolving folic acid and m-phenylenediamine in water, and carrying out heating for a reaction; and 2) after the reaction, performing centrifuging and silica gel column chromatography, collecting a yellow-green fluorescent part, transferring the yellow-green fluorescent part into water after rotary evaporation and then carrying out lyophilization. The carbon dot prepared in the invention emits green light under ultraviolet excitation, has large Stokes shift,is consistent with the existing commercial 405 laser at the optimal excitation position, and can be well applied to a laser confocal microscope.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials and biological imaging, and in particular relates to a fluorescent carbon dot for cell nucleus staining and its application and method in cell nucleus fluorescence imaging. Background technique [0002] Cells are important carriers in life activities, and the study of cells and foreign substances can help us understand the transportation, metabolism and toxicity of cells. Among them, the nucleus is particularly important for many processes of cells, such as metabolism, generation and inheritance. However, relative to its vital importance, the nucleus is relatively poorly studied. The staining of the nucleus is the first step, he can show the shape of the nucleus, and then the transport of the nucleus and the transport of reagents to the nucleus can be studied. With the rapid development of fluorescence microscopy, fluorescent staining of cell nuclei has become extremely common due to its v...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C09K11/65C01B32/15B82Y20/00B82Y40/00G01N21/64
CPCB82Y20/00B82Y40/00C09K11/65C01B32/15G01N21/6486
Inventor 李朝辉刘海芳孙远强杨杰屈凌波
Owner ZHENGZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com