A fluorescent carbon dot for nuclear staining and its application and method in nuclear imaging

A fluorescent carbon dot and fluorescent imaging technology, applied in the field of nanomaterials and biological imaging, can solve the problems of long incubation time and difficult cell nucleus imaging, and achieve the effects of short incubation time, cheap devices and reagents, and low incubation concentration

Active Publication Date: 2021-06-04
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

When the carbon dots invented at present are used for cell imaging, due to the size of the material, surface charge and other properties, it is difficult to apply to the imaging of the nucleus. Most of the invented carbon dots can only enter the cytoplasm, mitochondria and other parts of the cell, and require longer incubation time

Method used

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  • A fluorescent carbon dot for nuclear staining and its application and method in nuclear imaging
  • A fluorescent carbon dot for nuclear staining and its application and method in nuclear imaging
  • A fluorescent carbon dot for nuclear staining and its application and method in nuclear imaging

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Embodiment 1

[0029] A kind of fluorescent carbon dots for cell nucleus staining, the carbon dots are prepared by the following steps:

[0030] 1) Dissolve 5 mg of folic acid and 13 mg of m-phenylenediamine in 5000 mg of deionized water, place it in a hydrothermal reactor and heat it to 200 °C for 12 hours; in other embodiments, the mass ratio of folic acid to m-phenylenediamine is 1 : (2-20) can be used, the amount of deionized water can be enough to dissolve the raw materials;

[0031] 2) After the reaction, centrifuge (remove large particle agglomerates) to take the upper layer solution and pass it through silica gel column chromatography to collect the yellow-green fluorescent part, rotate it (remove the organic solvent), transfer it to distilled water, and freeze-dry it to obtain fluorescent carbon dots; The mobile phase used in silica gel column chromatography is a mixture of methanol and ethyl acetate in a volume ratio of 1:1; the specific operation of freeze-drying is: first freeze ...

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Abstract

A fluorescent carbon dot for cell nucleus staining. The carbon dot is prepared by the following steps: 1) dissolving folic acid and m-phenylenediamine in water and heating for reaction; 2) after the reaction, centrifugation, and silica gel column chromatography to collect yellow The green fluorescent part was evaporated, transferred to water, and freeze-dried. The carbon dots prepared by the invention emit green light under ultraviolet excitation, have a large Stokes shift, and the optimal excitation position is consistent with the existing commercial 405 laser, and can be well applied to laser confocal microscopy.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials and biological imaging, and in particular relates to a fluorescent carbon dot for cell nucleus staining and its application and method in cell nucleus fluorescence imaging. Background technique [0002] Cells are important carriers in life activities, and the study of cells and foreign substances can help us understand the transport, metabolism and toxicity of cells. Among them, the nucleus is particularly important for many processes of the cell, such as metabolism, passage and inheritance. However, relative to the crucial importance of the nucleus, its research is relatively lacking. Staining the nucleus is the first step, it can show the morphology of the nucleus, after which the transport of the nucleus and the transport of reagents to the nucleus can be studied. With the rapid development of fluorescence microscopy, fluorescent staining of cell nuclei has become extremely common due t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/65C01B32/15B82Y20/00B82Y40/00G01N21/64
CPCB82Y20/00B82Y40/00C09K11/65C01B32/15G01N21/6486
Inventor 李朝辉刘海芳孙远强杨杰屈凌波
Owner ZHENGZHOU UNIV
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