129results about How to "Short incubation time" patented technology

This invention relates to organometallic compounds comprising at least one metal or metalloid and at least one substituted anionic 6 electron donor ligand having sufficient substitution (i) to impart decreased carbon concentration in a film or coating produced by decomposing said compound, (ii) to impart decreased resistivity in a film or coating produced by decomposing said compound, or (iii) to impart increased crystallinity in a film or coating produced by decomposing said compound. The organometallic compounds are useful in semiconductor applications as chemical vapor or atomic layer deposition precursors for film depositions.

The invention relates to a method for quick cultivation of long-stem standard rose using cutting with long shoots. A stock of the long-stem standard rose to be cultivated by the method is a shoot of rosa multiflora Thunb. var. carnea Thory, which is dipped with rooting powder for cuttage, the rooting powder is made of IBA (indole-3-butyric acid) 0.8-1.0% and thiophanate-methyl 2-5%, mass water content of substrate soil is maintained to be 60-65% after cuttage, relative humidity of air in a seed bed is 60-80%, the relative humidity of the air is reduced to 50-60% after a cutting seedling rootsand sprouts, daytime temperature is controlled to be 25-26 DEG C, night temperature is controlled to be 10-14 DEG C, a black shade with light transmittance 50% is used for covering from 11 O'clock to17 O'clock in two weeks after rooting and sprouting, the black shade is removed after two weeks, daytime temperature is then controlled to be 26-28 DEG C, and the rose shoot or bud is grafted to the stock. The stock has average height 120cm and only requires 135-150 days, and cultivation of the long-stem standard rose only requires 225-270 days, so that production cycle is short.

An insert for a centrifuge tube suitable for use in density gradient separation is described. The insert includes a member sized to fit within the tube for dividing the tube into a top portion and a bottom portion. Optionally the insert has a support extending or depending from the member for positioning the member within the tube. At least two openings are located on the member so that a first opening is closer to a bottom end of the tube relative to a second opening when the insert is positioned in the centrifuge tube. Also described are methods for separating a target population of cells from a sample using the insert for a centrifuge tube.

The present invention relates to a method for the determination of the presence and amount of DNA in a sample. The method is based on the use of a nucleic acid template dependent enzyme in combination with a random primer to generate an enzymatic product which incorporates a binding species and a detectable species covalently linked.

A method for identifying a platelet population, preferably a population of immature, reticulated platelets, in a biological sample involves incubating the biological sample for less than minutes with at least one labeled, ligand (e.g., monoclonal antibody) that binds to an epitope or antigen on platelets and with a nucleic acid dye. In one embodiment, the dye is Acridine Orange and the label on the ligand is PE-Cy7. The sample is then analyzed and one or more platelet populations is rapidly identified or quantified by passing the incubated sample through a sensing region of a flow cytometer. In one embodiment, this method occurs without a washing or physical cell separation step. The incubated sample is irradiated with a laser light source, and fluorescence of the labeled ligand and the nucleic acid dye are measured along with at least one additional parameter, e.g., light scatter, direct current, axial light loss, opacity, radio frequency, and fluorescence. These parameters are used to identify qualitatively or quantitatively the platelet populations in the sample. This rapid analytic method is particularly valuable in clinical situations where either low platelet counts or interfering conditions lead to inaccuracies of the platelet measurement. This method is suitable for performance in an automated hematology analyzer.

The invention provides a taxus chinensis var mairei cultivation method. The taxus chinensis var mairei cultivation method comprises the following steps: firstly carrying out tissue culture on taxus chinensis var mairei so as to obtain test-tube plantlets, hardening off the test-tube plantlets, and then carrying out cuttage. The taxus chinensis var mairei cultivation method has the beneficial effects that 1. selenium is added to a tissue culture medium and nutrient soil, and the growth of the taxus chinensis var mairei can be effectively promoted in a tissue cultivation process and in a cuttage process; 2. the tissue cultivation method is simple to operate, the technical scheme is mature, the cultivation time is short, the cost is low, the survival rate is high, the rooting percentage is high, and the bottle-out transplantation can be realized in short time; 3. the tissue cultivation method is capable of maintaining good characters of original varieties and lays a good foundation for taxus chinensis var mairei breeding and agricultural modernization; 4. the cuttage method is simple in operation steps, high in survival rate and applicable to most of regions in Southern China and has a good application effect.

Cellfree adipose tissue extracts, implants including the same and methods for the preparation thereof. The extracts and implants are capable of inducing adipogenesis and angiogenesis and are, thus, useful in applications including soft tissue repair and engineering and angiogenesis induction, e.g., in wound healing, treatment of burn injuries and ischemic conditions.

The present invention relates to a method for the determination of the presence and amount of DNA in a sample. The method is based on the use of a nucleic acid template dependent enzyme in combination with a random primer to generate an enzymatic product which incorporates a binding species and a detectable species covalently linked.

The invention belongs to the field of plant cultivation, and particularly relates to a cultivation method of organic broomcorn for making wine. The cultivation method of the organic broomcorn for making wine comprises the following steps: a, treating seeds; b, sowing; c, transplanting; and d, harvesting. By the adoption of the cultivation method, the quality of the broomcorn is good, yield is high, and cultivation time is short.

Nanoparticles as described herein are configured to bind to bacterial contaminants, such as Gram positive bacteria, Gram negative bacteria, and endotoxins. The nanoparticles include a core comprising a magnetic material; and a plurality of ligands attached to the core. The ligands include, for example, bis(dipicolylamine) (“DPA”) coordinated with a metal ion, e.g., Zn2+ or Cu2+, to form, e.g., bis-Zn-DPA or bis-Cu-DPA, which can bind to the bacterial contaminants. The nanoparticles can be included in compositions for use in methods and systems to separate bacterial contaminants from liquids, such as liquids, such as blood, e.g., whole or diluted blood, buffer solutions, albumin solutions, beverages for human and / or animal consumption, e.g., drinking water, liquid medications for humans and / or animals, or other liquids.

The invention belongs to the field of food or healthcare products and aims at providing a brand new tall gastrodia tuber culture method, tall gastrodia tuber liquor and a tall gastrodia tuber culture method by using small-caliber containers. The tall gastrodia tuber culture method comprises the following steps of: 1) preparing culture medium; and 2) culturing tall gastrodia tubers. The key points are focused on the selection of tall gastrodia tuber culture medium and the control of culture conditions. The culture medium comprises potato culture medium, tree sawdust and halimasch. The preparation method of the culture medium comprises the following steps of: adding the tree sawdust into culture containers and adding the potato culture medium to submerge the tree sawdust; conducting disinfection; and inoculating and culturing the halimasch. By adopting the tall gastrodia tuber culture method, the period from the seeding to the harvesting of the tall gastrodia tubers is approximate 4-6 months; and the tall gastrodia tuber culture method has the advantages that the culture period is short, the survival rate is high, the diameter of the tall gastrodia tubers can reach 6-8cm and the like. The minimum diameter of the tall gastrodia tubers in the containers which contain the tall gastrodia tuber liquor is larger than the diameter of the mouths of the containers, the tall gastrodia tubers in the containers are intact and cannot be integrally taken out from the mouths of the containers, the containers are novel and interesting and can attract the curiosity of the public and a new product is provided for the public.

This invention relates to organometallic compounds represented by the formula M(NR1R2)x wherein M is a metal or metalloid, R1 is the same or different and is a hydrocarbon group or a heteroatom-containing group, R2 is the same or different and is a hydrocarbon group or a heteroatom-containing group; R1 and R2 can be combined to form a substituted or unsubstituted, saturated or unsaturated cyclic group; R1 or R2 of one (NR1R2) group can be combined with R1 or R2 of another (NR1R2) group to form a substituted or unsubstituted, saturated or unsaturated cyclic group; x is equal to the oxidation state of M; and wherein said organometallic compound has (i) a steric bulk sufficient to maintain a monomeric structure and a coordination number equal to the oxidation state of M with respect to anionic ligands, and (ii) a molecular weight sufficient to possess a volatility suitable for vapor deposition.; a process for producing the organometallic compounds, and a method for producing a film or coating from organometallic precursor compounds.

This invention relates to organoaluminum precursor compounds represented by the formula: wherein R1, R2, R3 and R4 are the same or different and each represents hydrogen or an alkyl group having from 1 to about 3 carbon atoms, and R5 represents an alkyl group having from 1 to about 3 carbon atoms. This invention also relates to processes for producing the organoaluminum precursor compounds and a method for producing a film or coating from the organoaluminum precursor compounds.

The invention belongs to the technical field of culture and particularly relates to a method for breeding a new local stress-resistant meat rabbit species. According to the method, Sichuan white rabbits and German synthetic white rabbits are taken as breeding materials, and the new local stress-resistant meat rabbit species is bred through a grading hybridization method. The new meat rabbit species bred through the method has the advantages of the Sichuan white rabbits and the advantages of the German synthetic white rabbits and is strong in stress resistance, and the fattening survival rate reaches over 98%; bucks are strong in sexual desire and high in semen quality; does are obvious in estrus and easy in mating, the blood-nest mating pregnancy ratio reaches up to 82%, the number of annual production births is over 7-8 under the condition of artificially-assisted mating, the continuous production performance is good, and the new meat rabbit species has very strong adaptability to the climate environment and culture characteristics of the southwest of China.

The invention belongs to the technical field of breeding of meat rabbits, and relates to the breeding method of cold-resistant rabbits. The method comprises the first step of breeding cold-resistant breeding rabbits the Soviet union Chinchillas introduced from Russia and Vienna azure purebred rabbits for four generations respectively to form specialized strains; the second step of choosing Chinchillas doe and Vienna azure buck to conduct hybrid to produce an F1 generation; the third step of choosing doe which are expanding in skin, obvious in oestrus and good in maternity in the F1 generation to conduct backcross on the Vienna azure buck to generate an F2 generation; the fourth step of choosing buck which are large in physique, good in meat yield performance, and large in hair density in the F2 generation with doe which are expanding in skin, obvious in oestrus and good in maternity to conduct crossing to obtain an F3 generation; the fifth step of repeating the fourth step till a meat rabbit new strain in which the rabbits are basically consistent in body form and appearance, stable in genetic performance, and good in cold resistant performance is bred. The breeding method of the cold-resistant rabbit is simple and feasible, the breeding time is short, the cost is low, the new-strain rabbits are strong in reproduction capability, large in growth speed, high in adaptability and high in economic benefit.

The invention provides a high-production-performance black meat rabbit new strain breeding method. The breeding method comprises the steps that firstly, local male black rabbits are mated with femalewhite meat rabbits to obtain an F1 generation; the black male rabbits in the F1 generation are selected for backcrossing with the female white meat rabbits to obtain an F2 generation; the black male rabbits in the F2 generation are selected for backcrossing with the female white meat rabbits again to obtain an F3 generation; the black rabbits in the F3 generation are selected for crossing to generate an F4 generation; black male and female rabbits in the F4 generation are selected for gene identification, and homozygotic black male and female rabbits are selected to form a basic group; the basic group is subjected to atresia subculture to breed 3-4 generations, and a high-production-performance black meat rabbit new strain consistent in body shape and appearance can be bred. According to the black meat rabbit new strain bred through the breeding method, in the pure breeding and hybrid production process, it can be ensured that the hair colors of commercial generation rabbits are consistent. Meanwhile, the production performance and uniformity are relatively high, and the method is suitable for modern meat rabbit matching production.

This invention relates to organometallic compounds represented by the formula (L1)xM(L2)y wherein M is a metal or metalloid, L1 and L2 are different and are each a hydrocarbon group or a heteroatom-containing group; x is a value of at least 1; y is a value of at least 1; x+y is equal to the oxidation state of M; and wherein (i) L1 has a steric bulk sufficiently large such that, due to steric hinderance, x cannot be a value equal to the oxidation state of M, (ii) L2 has a steric bulk sufficiently small such that, due to lack of steric hinderance, y can be a value equal to the oxidation state of M only in the event that x is not a value of at least 1, and (iii) L1 and L2 have a steric bulk sufficient to maintain a heteroleptic structure in which x+y is equal to the oxidation state of M; a process for producing the organometallic compounds, and a method for producing a film or coating from organometallic precursor compounds.

The invention provides a method for inducing young elaphe carinata to eat non-living feeds, and belongs to the technical field of snake-raising methods. The method comprises the following steps of: 1, hatching elaphe carinata eggs with vermiculites; 2, collecting young elaphe carinata in 4 to 8 hours after hatching, and breeding the young elaphe carinata in a breeding room; 4, from the fourth week, feeding the young elaphe carinata with living suckling mice; 4, on the fourth to sixth day after the young elaphe carinata get feeding for the first time, feeding the young elaphe carinata with the mixture of unfrozen sliced pork and the suckling mice; and 5, feeding the young elaphe carinata with the mixture of the unfrozen sliced pork and the suckling mice every 4 to 6 days, wherein during each feeding, the content of the suckling mouse in the feed gradually decreases, the content of the sliced pork in the feed gradually increases, and the young elaphe carinata is completely fed by sliced pork in one month after the induction begins. The newly hatched young elaphe carinata are induced to actively eat low-price non-living open baits which are easy to process and store and can be added with additives easily by utilizing the imprinting behaviors of animals; in addition, the feeding method is independent of limitation of seasons, easy to operate and low in feeding cost; and the survival rate of young elaphe carinata is high.

The present invention discloses a temperature and humidity control method of trionyx sinensis incubation room. The temperature and humidity control method comprises the following control steps: (1) providing a heating apparatus, a humidifying apparatus and a ventilation apparatus in the incubation room, and providing a water sprayiing apparatus at the roof of the incubation room; and (2) by virtue of a signal collected by the temperature sensor and humidity sensor provided in the incubation room, controlling the temperature of the incubation room at 28-30 DEG C and the humidity at 78%-85% by controlling the heating apparatus, the humidifying apparatus, the ventilation apparatus and the water spraying apparatus. According to the temperature and humidity control method of trionyx sinensis incubation room disclosed by the invention, after trionyx sinensis eggs in a trionyx sinensis pond are collected in a unified mode, the method is utilized to perform incubation; and the method has characteristics of a high incubation rate and short incubation time.

A process for the bio-neutralisation of red mud, the process including: feeding an alkaline red mud into a bio-digester; feeding biomass including insoluble organic matter into the bio-digester, the biomass supporting a microbial consortium; mediating the digestion of the biomass in the bio-digester or through a train of bio-digesters with microbes in the microbial consortium, to thereby produce organic acid(s) which neutralise alkalinity of the red mud and reduce pH of the red mud; producing a bio-neutralised red mud product having a pH of 10 or less.

The invention discloses a method for accelerating germination of seeds of cyclocarya paliurus in the southern region in Shandong province, and belongs to the field of tree planting. The method is characterized by comprising the first step of soaking the seeds, the second step of carrying out vibration decompression nitrogen-enriching process and the third step of accelerating germination. Compared with the prior art, the method can accelerate the germination of the seeds of sassafras, improve the cold-resisting property of seedlings and enhance the adaptation ability of the seeds of the sassafras to bad environments, and accordingly a high germination rate and a high seedling survival rate are achieved.

The invention discloses a rhubarb floating seedling and transplanting method and relates to the technical field of plantation of Chinese medicinal herbs. The method includes the steps: firstly, adopting a greenhouse facility; secondly, seeding; thirdly, cultivating and fertilizing; fourthly, transplanting. The step of sowing includes: 1, bud forcing treatment: soaking for 6-8 hours in warm water at the temperature of 18-20 DEG C, covering with gauze, and flushing with cold water for 2-3 times every day; 2, filling of seedling raising plates: filling a prepared culture medium into the seedling raising plates, and punching for standby application by a puncher; 3, sowing: sowing seeds into holes, wherein one to two seeds are sowed in each hole, and sowing depth is 0.5cm. The step of cultivating and fertilizing includes: water injection, plate placement, environment management, seedling emergence and fertilization. Transplanting is generally carried out in a 'three leaf and one core' period, namely each seedling grows out three main leaves and one plant core, and transplanting is carried out in spring, summer and autumn. By the rhubarb floating seedling and transplanting method, the problems of limits of physioclimate conditions, seedling supply time concentration, low seedling yield per mu, high labor demand, high seed consumption, low germination percentage of seeds sowed in land and the like in rhubarb plantation are solved.

A method for quickly breeding a new aromatic rice variety includes the following steps: (1) parent selection; (2) sexual hybridization and seed harvest; (3) hybrid seed growing and harvest; (4) seeding and sampling; (5) leaf aroma testing; (6) selective growing and seed harvest; (7) finalization; (8) seed aroma testing. When the method is utilized to breed the new aromatic rice variety, the breeding time is short, the success rate is high, and the aroma segregation of the rice is little; and in addition, the method is easy to operate, the workload is less, the new aromatic rice variety can be predictably and directionally bred, moreover, the purity of the obtained variety is high, and the breeding effect can be greatly enhanced.

This invention relates to organometallic precursor compounds represented by the formula i-PrN═Ta(NR1R2)3 wherein R1 and R2 are the same or different and are alkyl having from 1 to 3 carbon atoms, provided that (i) when R1 is ethyl, then R2 is other than ethyl and (ii) when R2 is ethyl, then R1 is other than ethyl, and a method for producing a film, coating or powder from the organometallic precursor compounds.

The invention discloses a Ficus carica rapid vegetative propagation method, which can solve the problem that the cutting and transplanting survival rate is low during cutting seedling cultivation of the Ficus carica, the transplanted seedlings are not health and strong, and the cultivation time is long in the prior art. The Ficus carica rapid vegetative propagation method is cutting seedling cultivation and includes the following steps: cutting seedling bed preparation, cutting medium preparation, cutting scion selection, cutting scion pruning, cutting scion chemical solution treatment, cutting, after-cutting management. In an early stage after cutting, leaves of cutting scions are sprayed with water for many times each day till the leaves are wet, and the cutting scions are transplanted. The Ficus carica rapid vegetative propagation method is simple, is easy to operate, can perform propagation of the Ficus carica, is high in cutting and transplanting survival rate, is healthy and strong in transplanted seedlings, and is short in cultivation time.

The invention discloses a method for rapidly culturing anaerobic granular sludge applicable to pharmaceutical wastewater treatment. The method specifically comprises the following steps: according to the method disclosed by the invention, performing fermentation treatment on residual sludge by adopting a mode of feeding microbial preparations, putting the treated sludge into an anaerobic reactor, mixing with anaerobic flocculent sludge to culture, and feeding nutrient elements such as N, P, Fe and Mn in the culture process, thereby obtaining the anaerobic granular sludge after about 71-98 days. The anaerobic granular sludge formation time can be greatly shortened. In addition, the anaerobic granular sludge prepared by using the method can be adopted to treat pharmaceutical wastewater of antibiotics, and has a relatively high COD removal rate and a good antibiotic degradation effect.

The invention belongs to the technical field of plant cultivation, and particularly relates to a method for quickly cultivating salt-tolerant kandelia candel. The method for quickly cultivating salt-tolerant kandelia candel comprises the steps that a trans-regional seed collection mode is adopted, then mature kandelia candel hypocotyls are placed in self-made nutrient soil and cultivated to seedlings, the seedlings are transplanted to a seedling bed and cultivated to big seedlings, the big seedlings are cultivated in bags for a period of time after root breakage treatment, and then the big seedlings can be carried and transplanted for afforestation. For the big kandelia candel seedlings cultivated by means of the method for quickly cultivating salt-tolerant kandelia candel, the plant height is 1.5-1.8 m, the crown breadth is 50-75 cm, the salt tolerance degree reaches 35%o or above, the transplanting survival rate reaches 96% or above, planting afforestation can be conducted in deep-water beaches with high salinity, and multiple functions of kandelia candel for protecting banks, reducing waves, reducing and resisting disasters, optimizing the coastal environment, maintaining biodiversity and the like can be fully exerted.

The invention belongs to the technical field of a miniascape cultivation method, in particular to a miniascape root-inoculation cultivation method, which solves the problems that the traditional miniascape cultivation takes a long time and labor and material consumption are large. The steps of the miniascape root-inoculation cultivation method comprise: 1) the trunk or branch on the growing tree are selected to carry out the annular barking to the bottom thereof and then to cut off a sieve tube of a phloem; 2) a plurality of notably developed roots at the tree root can be selected to graft the roots at the upper part of the barking openings; 3) a cylinder container is sleeved and arranged at the grafting position, nutrition soils are filled in the container and the container is closed, a plurality of breathing holes are arranged on the container wall so as to keep the moisture in the container; 4) the cultivated trunk or branch are cut off until thickset roots are reproduced in the container to plant into a basin after removing the container; the trunk or branch are shaped and curd for 20 to 30 days. The invention takes short cultivation time for only 2 to 3 months with low consumption cost.