129results about How to "Short incubation time" patented technology

This invention relates to organometallic compounds comprising at least one metal or metalloid and at least one substituted anionic 6 electron donor ligand having sufficient substitution (i) to impart decreased carbon concentration in a film or coating produced by decomposing said compound, (ii) to impart decreased resistivity in a film or coating produced by decomposing said compound, or (iii) to impart increased crystallinity in a film or coating produced by decomposing said compound. The organometallic compounds are useful in semiconductor applications as chemical vapor or atomic layer deposition precursors for film depositions.

An insert for a centrifuge tube suitable for use in density gradient separation is described. The insert includes a member sized to fit within the tube for dividing the tube into a top portion and a bottom portion. Optionally the insert has a support extending or depending from the member for positioning the member within the tube. At least two openings are located on the member so that a first opening is closer to a bottom end of the tube relative to a second opening when the insert is positioned in the centrifuge tube. Also described are methods for separating a target population of cells from a sample using the insert for a centrifuge tube.

A method for identifying a platelet population, preferably a population of immature, reticulated platelets, in a biological sample involves incubating the biological sample for less than minutes with at least one labeled, ligand (e.g., monoclonal antibody) that binds to an epitope or antigen on platelets and with a nucleic acid dye. In one embodiment, the dye is Acridine Orange and the label on the ligand is PE-Cy7. The sample is then analyzed and one or more platelet populations is rapidly identified or quantified by passing the incubated sample through a sensing region of a flow cytometer. In one embodiment, this method occurs without a washing or physical cell separation step. The incubated sample is irradiated with a laser light source, and fluorescence of the labeled ligand and the nucleic acid dye are measured along with at least one additional parameter, e.g., light scatter, direct current, axial light loss, opacity, radio frequency, and fluorescence. These parameters are used to identify qualitatively or quantitatively the platelet populations in the sample. This rapid analytic method is particularly valuable in clinical situations where either low platelet counts or interfering conditions lead to inaccuracies of the platelet measurement. This method is suitable for performance in an automated hematology analyzer.

The invention provides a taxus chinensis var mairei cultivation method. The taxus chinensis var mairei cultivation method comprises the following steps: firstly carrying out tissue culture on taxus chinensis var mairei so as to obtain test-tube plantlets, hardening off the test-tube plantlets, and then carrying out cuttage. The taxus chinensis var mairei cultivation method has the beneficial effects that 1. selenium is added to a tissue culture medium and nutrient soil, and the growth of the taxus chinensis var mairei can be effectively promoted in a tissue cultivation process and in a cuttage process; 2. the tissue cultivation method is simple to operate, the technical scheme is mature, the cultivation time is short, the cost is low, the survival rate is high, the rooting percentage is high, and the bottle-out transplantation can be realized in short time; 3. the tissue cultivation method is capable of maintaining good characters of original varieties and lays a good foundation for taxus chinensis var mairei breeding and agricultural modernization; 4. the cuttage method is simple in operation steps, high in survival rate and applicable to most of regions in Southern China and has a good application effect.

Cellfree adipose tissue extracts, implants including the same and methods for the preparation thereof. The extracts and implants are capable of inducing adipogenesis and angiogenesis and are, thus, useful in applications including soft tissue repair and engineering and angiogenesis induction, e.g., in wound healing, treatment of burn injuries and ischemic conditions.

The invention belongs to the field of food or healthcare products and aims at providing a brand new tall gastrodia tuber culture method, tall gastrodia tuber liquor and a tall gastrodia tuber culture method by using small-caliber containers. The tall gastrodia tuber culture method comprises the following steps of: 1) preparing culture medium; and 2) culturing tall gastrodia tubers. The key points are focused on the selection of tall gastrodia tuber culture medium and the control of culture conditions. The culture medium comprises potato culture medium, tree sawdust and halimasch. The preparation method of the culture medium comprises the following steps of: adding the tree sawdust into culture containers and adding the potato culture medium to submerge the tree sawdust; conducting disinfection; and inoculating and culturing the halimasch. By adopting the tall gastrodia tuber culture method, the period from the seeding to the harvesting of the tall gastrodia tubers is approximate 4-6 months; and the tall gastrodia tuber culture method has the advantages that the culture period is short, the survival rate is high, the diameter of the tall gastrodia tubers can reach 6-8cm and the like. The minimum diameter of the tall gastrodia tubers in the containers which contain the tall gastrodia tuber liquor is larger than the diameter of the mouths of the containers, the tall gastrodia tubers in the containers are intact and cannot be integrally taken out from the mouths of the containers, the containers are novel and interesting and can attract the curiosity of the public and a new product is provided for the public.

This invention relates to organometallic compounds represented by the formula M(NR₁R₂)_x wherein M is a metal or metalloid, R₁ is the same or different and is a hydrocarbon group or a heteroatom-containing group, R₂ is the same or different and is a hydrocarbon group or a heteroatom-containing group; R₁ and R₂ can be combined to form a substituted or unsubstituted, saturated or unsaturated cyclic group; R₁ or R₂ of one (NR₁R₂) group can be combined with R₁ or R₂ of another (NR₁R₂) group to form a substituted or unsubstituted, saturated or unsaturated cyclic group; x is equal to the oxidation state of M; and wherein said organometallic compound has (i) a steric bulk sufficient to maintain a monomeric structure and a coordination number equal to the oxidation state of M with respect to anionic ligands, and (ii) a molecular weight sufficient to possess a volatility suitable for vapor deposition.; a process for producing the organometallic compounds, and a method for producing a film or coating from organometallic precursor compounds.

This invention relates to organoaluminum precursor compounds represented by the formula:

wherein R₁, R₂, R₃ and R₄ are the same or different and each represents hydrogen or an alkyl group having from 1 to about 3 carbon atoms, and R₅ represents an alkyl group having from 1 to about 3 carbon atoms. This invention also relates to processes for producing the organoaluminum precursor compounds and a method for producing a film or coating from the organoaluminum precursor compounds.

The invention belongs to the technical field of culture and particularly relates to a method for breeding a new local stress-resistant meat rabbit species. According to the method, Sichuan white rabbits and German synthetic white rabbits are taken as breeding materials, and the new local stress-resistant meat rabbit species is bred through a grading hybridization method. The new meat rabbit species bred through the method has the advantages of the Sichuan white rabbits and the advantages of the German synthetic white rabbits and is strong in stress resistance, and the fattening survival rate reaches over 98%; bucks are strong in sexual desire and high in semen quality; does are obvious in estrus and easy in mating, the blood-nest mating pregnancy ratio reaches up to 82%, the number of annual production births is over 7-8 under the condition of artificially-assisted mating, the continuous production performance is good, and the new meat rabbit species has very strong adaptability to the climate environment and culture characteristics of the southwest of China.

The invention belongs to the technical field of breeding of meat rabbits, and relates to the breeding method of cold-resistant rabbits. The method comprises the first step of breeding cold-resistant breeding rabbits the Soviet union Chinchillas introduced from Russia and Vienna azure purebred rabbits for four generations respectively to form specialized strains; the second step of choosing Chinchillas doe and Vienna azure buck to conduct hybrid to produce an F1 generation; the third step of choosing doe which are expanding in skin, obvious in oestrus and good in maternity in the F1 generation to conduct backcross on the Vienna azure buck to generate an F2 generation; the fourth step of choosing buck which are large in physique, good in meat yield performance, and large in hair density in the F2 generation with doe which are expanding in skin, obvious in oestrus and good in maternity to conduct crossing to obtain an F3 generation; the fifth step of repeating the fourth step till a meat rabbit new strain in which the rabbits are basically consistent in body form and appearance, stable in genetic performance, and good in cold resistant performance is bred. The breeding method of the cold-resistant rabbit is simple and feasible, the breeding time is short, the cost is low, the new-strain rabbits are strong in reproduction capability, large in growth speed, high in adaptability and high in economic benefit.

The invention provides a high-production-performance black meat rabbit new strain breeding method. The breeding method comprises the steps that firstly, local male black rabbits are mated with femalewhite meat rabbits to obtain an F1 generation; the black male rabbits in the F1 generation are selected for backcrossing with the female white meat rabbits to obtain an F2 generation; the black male rabbits in the F2 generation are selected for backcrossing with the female white meat rabbits again to obtain an F3 generation; the black rabbits in the F3 generation are selected for crossing to generate an F4 generation; black male and female rabbits in the F4 generation are selected for gene identification, and homozygotic black male and female rabbits are selected to form a basic group; the basic group is subjected to atresia subculture to breed 3-4 generations, and a high-production-performance black meat rabbit new strain consistent in body shape and appearance can be bred. According to the black meat rabbit new strain bred through the breeding method, in the pure breeding and hybrid production process, it can be ensured that the hair colors of commercial generation rabbits are consistent. Meanwhile, the production performance and uniformity are relatively high, and the method is suitable for modern meat rabbit matching production.

The present invention discloses a temperature and humidity control method of trionyx sinensis incubation room. The temperature and humidity control method comprises the following control steps: (1) providing a heating apparatus, a humidifying apparatus and a ventilation apparatus in the incubation room, and providing a water sprayiing apparatus at the roof of the incubation room; and (2) by virtue of a signal collected by the temperature sensor and humidity sensor provided in the incubation room, controlling the temperature of the incubation room at 28-30 DEG C and the humidity at 78%-85% by controlling the heating apparatus, the humidifying apparatus, the ventilation apparatus and the water spraying apparatus. According to the temperature and humidity control method of trionyx sinensis incubation room disclosed by the invention, after trionyx sinensis eggs in a trionyx sinensis pond are collected in a unified mode, the method is utilized to perform incubation; and the method has characteristics of a high incubation rate and short incubation time.

A process for the bio-neutralisation of red mud, the process including: feeding an alkaline red mud into a bio-digester; feeding biomass including insoluble organic matter into the bio-digester, the biomass supporting a microbial consortium; mediating the digestion of the biomass in the bio-digester or through a train of bio-digesters with microbes in the microbial consortium, to thereby produce organic acid(s) which neutralise alkalinity of the red mud and reduce pH of the red mud; producing a bio-neutralised red mud product having a pH of 10 or less.

The invention discloses a method for accelerating germination of seeds of cyclocarya paliurus in the southern region in Shandong province, and belongs to the field of tree planting. The method is characterized by comprising the first step of soaking the seeds, the second step of carrying out vibration decompression nitrogen-enriching process and the third step of accelerating germination. Compared with the prior art, the method can accelerate the germination of the seeds of sassafras, improve the cold-resisting property of seedlings and enhance the adaptation ability of the seeds of the sassafras to bad environments, and accordingly a high germination rate and a high seedling survival rate are achieved.

The invention discloses a rhubarb floating seedling and transplanting method and relates to the technical field of plantation of Chinese medicinal herbs. The method includes the steps: firstly, adopting a greenhouse facility; secondly, seeding; thirdly, cultivating and fertilizing; fourthly, transplanting. The step of sowing includes: 1, bud forcing treatment: soaking for 6-8 hours in warm water at the temperature of 18-20 DEG C, covering with gauze, and flushing with cold water for 2-3 times every day; 2, filling of seedling raising plates: filling a prepared culture medium into the seedling raising plates, and punching for standby application by a puncher; 3, sowing: sowing seeds into holes, wherein one to two seeds are sowed in each hole, and sowing depth is 0.5cm. The step of cultivating and fertilizing includes: water injection, plate placement, environment management, seedling emergence and fertilization. Transplanting is generally carried out in a 'three leaf and one core' period, namely each seedling grows out three main leaves and one plant core, and transplanting is carried out in spring, summer and autumn. By the rhubarb floating seedling and transplanting method, the problems of limits of physioclimate conditions, seedling supply time concentration, low seedling yield per mu, high labor demand, high seed consumption, low germination percentage of seeds sowed in land and the like in rhubarb plantation are solved.

A method for quickly breeding a new aromatic rice variety includes the following steps: (1) parent selection; (2) sexual hybridization and seed harvest; (3) hybrid seed growing and harvest; (4) seeding and sampling; (5) leaf aroma testing; (6) selective growing and seed harvest; (7) finalization; (8) seed aroma testing. When the method is utilized to breed the new aromatic rice variety, the breeding time is short, the success rate is high, and the aroma segregation of the rice is little; and in addition, the method is easy to operate, the workload is less, the new aromatic rice variety can be predictably and directionally bred, moreover, the purity of the obtained variety is high, and the breeding effect can be greatly enhanced.

This invention relates to organometallic precursor compounds represented by the formula i-PrN═Ta(NR₁R₂)₃ wherein R₁ and R₂ are the same or different and are alkyl having from 1 to 3 carbon atoms, provided that (i) when R₁ is ethyl, then R₂ is other than ethyl and (ii) when R₂ is ethyl, then R₁ is other than ethyl, and a method for producing a film, coating or powder from the organometallic precursor compounds.

The invention discloses a method for rapidly culturing anaerobic granular sludge applicable to pharmaceutical wastewater treatment. The method specifically comprises the following steps: according to the method disclosed by the invention, performing fermentation treatment on residual sludge by adopting a mode of feeding microbial preparations, putting the treated sludge into an anaerobic reactor, mixing with anaerobic flocculent sludge to culture, and feeding nutrient elements such as N, P, Fe and Mn in the culture process, thereby obtaining the anaerobic granular sludge after about 71-98 days. The anaerobic granular sludge formation time can be greatly shortened. In addition, the anaerobic granular sludge prepared by using the method can be adopted to treat pharmaceutical wastewater of antibiotics, and has a relatively high COD removal rate and a good antibiotic degradation effect.