19 results about "Albumin solution" patented technology

The invention discloses a pH-responsive deformable dual-targeting nano protein and a preparation method and application thereof, the method comprises the following steps: activating carboxylated mannose through an EDC / NHS system, reacting with an albumin solution, and dialyzing and purifying to obtain mannose modified albumin; and mixing the mannose modified albumin with an inhibitor IRG1-IN-1, and loading the IRG1-IN-1 through a hydrophobic effect to obtain the mannose albumin nanoparticles, ferritin serves as a carrier, oxaliplatin is loaded through a solvent replacement method, and ferritin nanoparticles are obtained; cHO-PEG2k-CHO is used as a coupling agent, and the mannose albumin nanoparticles and the ferritin nanoparticles are crosslinked through a Schiff base reaction, so that the large-size pH-responsive dual-targeting nano-protein is formed. The nano protein has the characteristic of double-targeting separability, so that specific treatment can be realized, immune cell metabolism is interfered while tumor cells are killed, metabolic immunosuppression is blocked, and anti-tumor immunotherapy is promoted.

A composition and method for perfusing tissue. A perfusate is composed of an endothelial cell stabilizer. thrombocyte concentrate (TC). The perfusate may further comprise a protein solution that may be an albumin-based solution or fresh frozen plasma (FFP). The perfusate composition may further comprise red blood cells. The perfusate composition may be perfused through an organ that has been removed from an organ donor and is being prepared for transport and transplant into a recipient. The perfusate composition may be perfused through an organ using a machine perfusion device.

The application provides a cell drug cryopreservation preparation and a preparation method and application thereof, relates to the technical field of gene modified NK cell drug cryopreservation, and is characterized in that a basic cell cryopreservation solution is uniformly mixed with one or more of human blood albumin solution, polysaccharide solution, a surfactant, an apoptosis inhibitor and a compound electrolyte injection to obtain a cell drug cryopreservation preparation. The cryopreservation preparation and the cryopreservation method of the application have specific protective effects on gene modified NK cell drugs, and the cryopreservation preparation does not contain serum components, so that the possibility of animal pathogen pollution is reduced. The gene modified NK cell drugs are cryopreserved by using the cryopreservation preparation and the cryopreservation method of the application, so that the cell viability, the chimeric antigen receptor (CAR) positive rate, the long-term stability and the killing function on tumor cells can be maintained.

A preparation method of an albumin-paclitaxel twin drug nanoparticle. The preparation particularly relates to mixing albumin with water to prepare an albumin solution; mixing paclitaxel or a paclitaxel twin drug compound with an organic solvent to obtain a drug solution; and mixing the albumin solution with the drug solution, and performing ultrasonic dispersion, and then rotary evaporation and ultrafiltration to prepare an albumin-binding paclitaxel or albumin-paclitaxel twin drug nanoparticle. Disclosed is a use of the albumin-paclitaxel twin drug nanoparticle. Drug accumulation in tumors can be increased by means of using the special GP60-caveolin-SPARC transport mechanism of albumin, and the anti-tumor effect is good.

The embodiment of the present application proposes a preparation method of albumin nanoparticles and its use. The preparation method comprises: dissolving albumin in water to obtain an albumin solution; adding ethanol to the albumin solution to obtain a mixed solution; heating and stirring the mixed solution to obtain a supernatant, and dehydrating the supernatant to obtain albumin nanoparticles. The preparation method of the albumin nanoparticles provided in the embodiment of the present application adopts ultrapure water to dissolve the albumin, and adds ethanol to the albumin solution to destroy the hydration layer on the surface of the albumin, and forms a gel-like structure of the albumin by heating. At the same time, the stirring shear force and the cavitation effect of the gas and ethanol vapor in the water cause the albumin to break and polymerize to form nanoparticles. Therefore, the preparation method of the embodiment of the present application not only shortens the time for nanoparticle formation, but also reduces the amount of organic solvent used and simplifies the process, that is, albumin nanoparticles with low particle size and uniform particle size distribution can be obtained.

The present invention belongs to the technical field of pharmaceutical chemistry and biopharmaceuticals. Disclosed in the present invention are the preparation and use of an albumin-paclitaxel twin drug nanoparticle. The preparation particularly relates to mixing albumin with water to prepare an albumin solution; mixing paclitaxel or a paclitaxel twin drug compound with an organic solvent to obtain a drug solution; and mixing the albumin solution with the drug solution, and performing ultrasonic dispersion, and then rotary evaporation and ultrafiltration to prepare an albumin-binding paclitaxel or albumin-paclitaxel twin drug nanoparticle. With regard to the albumin-paclitaxel twin drug prepared in the present invention, drug accumulation in tumors can be increased by means of using the special GP60-caveolin-SPARC transport mechanism of albumin. Both in-vivo and in-vitro experiments prove that the albumin-paclitaxel twin drug nanoparticles of the present invention have a good anti-tumor effect.

The present invention discloses a bionic olfactory sensor and its preparation method and application, which belongs to the field of electrochemical biosensors. The working electrode of the bionic olfactory sensor is modified by a ZIF-8@SWCNT solution, a MOR1-1 / AuNPs / PB solution and a bovine serum albumin solution. The preparation method comprises: in situ growing a metal organic framework ZIF-8 on a single-walled carbon nanotube to obtain a ZIF-8@SWCNT solution, drop-coating the solution on the surface of a glassy carbon electrode, drying it in a dust-free environment, electro-depositing the MOR1-1 / AuNPs / PB solution on the electrode surface, refrigerating it until completely dry, and finally drop-coating a bovine serum albumin solution and refrigerating it until completely dry. The bionic olfactory sensor of the present invention can be used for the detection of hexanal content in grapes, and has high sensitivity, specificity and good stability.

The application belongs to the technical field of biological medicine material preparation, and particularly relates to albumin nanoparticles loaded with ferroptosis related drugs and a preparation method thereof. The ferroptosis related drug composition comprises porphyrin drugs, unsaturated fatty acid drugs-cystamine and sulfosuccinimidyl oleate (SSO). The porphyrin drugs, the unsaturated fatty acid drugs-cystamine and the SSO are slowly added dropwise into an albumin solution under stirring, the drugs enter the hydrophobic cavities of the albumin, and uniform nanoparticles are spontaneously formed. The obtained nanoparticles are small in size and uniform, can efficiently deliver drugs through passive targeting mediated by the EPR effect and active targeting mediated by the Gp60-Caveola-SPARC pathway, improve stability, reduce accumulation at non-target sites and systemic toxicity, amplify the effect of ferroptosis after combination compared with the ferroptosis related drugs used alone, and thus produce a synergistic anti-tumor effect.

This invention discloses a method for preparing flower-shaped silver powder. The method includes: 1. Preparing a silver nitrate solution to obtain solution A; 2. Preparing equal volumes of ascorbic acid solution and soy albumin solution, and mixing them to obtain solution B; 3. At room temperature, simultaneously and symmetrically adding solution A and anhydrous ethanol to solution B to react, allowing the generated silver particles to settle, be washed, and dried to obtain flower-shaped silver powder; applying this flower-shaped silver powder to low-temperature silver paste and Raman-enhanced substrates. The preparation method of this invention effectively controls the morphology of the reduced product silver powder through the synergistic effect of soy albumin and ethanol, preparing flower-shaped silver powder with a multi-level nanostructure. This flower-shaped silver powder has good dispersibility and high activity, and the preparation process is simple, efficient, environmentally friendly, and easy to scale up for production, making it suitable for fields such as electronics, catalysis, and biomedicine.

The application discloses a pH-responsive deformable double-targeting nano-protein and a preparation method and application thereof, and the method comprises the following steps: after carboxylated mannose is activated through an EDC / NHS system, the carboxylated mannose is reacted with an albumin solution, and mannose-modified albumin is obtained through dialysis purification; the mannose-modified albumin is mixed with an inhibitor IRG1-IN-1, and IRG1-IN-1 is loaded through hydrophobic interaction to obtain mannose albumin nanoparticles; ferritin is used as a carrier, and oxaliplatin is loaded through a solvent displacement method to obtain ferritin nanoparticles; CHO-PEG2k-CHO is used as a connecting agent, and the mannose albumin nanoparticles and the ferritin nanoparticles are crosslinked through a Schiff base reaction to form large-size pH-responsive double-targeting nano-proteins. The double-targeting separable characteristics of the nano-proteins can realize specific treatment, kill tumor cells, interfere with immune cell metabolism, block metabolic immunosuppression, and promote anti-tumor immunotherapy.

The application discloses a kind of albumin-paclitaxel twin drug nanoparticles and application, belong to the field of pharmaceutical chemistry and biopharmaceutical technology, specifically related to albumin is mixed with water, and albumin solution is prepared;Paclitaxel or paclitaxel twin drug compound is mixed with organic solvent, and drug solution is obtained;Albumin solution and drug solution are mixed, and after ultrasonic dispersion, spinning, ultrafiltration are carried out to prepare albumin binding type paclitaxel or albumin-paclitaxel twin drug nanoparticles.The albumin-paclitaxel twin drug prepared in the application can utilize the special GP60-cellar protein-SPARC transport mechanism of albumin, increase the accumulation of drug in tumor, and in vivo and in vitro experiments prove that the albumin-paclitaxel twin drug nanoparticles described in the application have good antitumor effect.

The invention relates to the technical field of medicines, in particular to a composition and application thereof in preparation of antitumor medicines. The composition disclosed by the invention consists of combretastatin A1, combretastatin A4 and albumin. The preparation method of the nano composition comprises the following steps: (1) diluting different albumin solutions to different concentrations by using double distilled water; (2) sufficiently and uniformly mixing combretastatin A1 and combretastatin A4 molecules with different concentrations with the albumin solution in the step (1); and (3) carrying out ultrasonic treatment on the mixture in the step (2) in a cell ultrasonic crusher at different powers, and centrifuging to obtain the albumin nano composition containing the combretastatin A1 and the combretastatin A4. The invention provides the preparation method of the anti-tumor nano composition, the method is simple, the anti-tumor effect is remarkable, and the clinical problems of drug resistance of pMMR type solid tumors to immunotherapy and the like can be effectively solved and overcome.

The present invention provides a composite nanoparticle, which is prepared by a method comprising the following steps: S1. Adding a potassium permanganate solution to a serum albumin solution, and carrying out a first reaction under stirring to obtain a first reaction product; S2. Subjecting the first reaction product to a first dialysis to obtain a first dialysis product; S3. Mixing the first dialysis product with a bilirubin solution, and carrying out a second reaction under oscillation to obtain a second reaction product; S4. Subjecting the second reaction product to a second dialysis to obtain a second dialysis product. The present invention also provides the use of the composite nanoparticle as described above in the preparation of a medicament for treating diabetes. Through the above technical solution, the present invention effectively improves the activity and success rate of stem cell transplantation by means of the composite nanoparticle, thereby achieving a better effect in treating diabetes.

The invention provides a preserving fluid for stably preserving umbilical cord mesenchymal stem cell exosomes for a long time, which belongs to the technical field of biotechnology and comprises the following components: a mannitol solution, a dimethyl sulfoxide solution, a human serum albumin solution and beta-nicotinamide mononucleotide. The invention provides a preserving fluid for stably preserving umbilical cord mesenchymal stem cell exosomes for a long time. The number and physicochemical properties of the umbilical cord mesenchymal stem cell exosomes are protected from being affected by freezing.

The invention discloses an anisotropic composite film based on albumin and a preparation method and application thereof, and belongs to the technical field of biomedical materials. The preparation method comprises the following steps: reducing an albumin solution by using a reducing agent and adjusting the pH value of the albumin solution by using organic alkali to obtain a denatured albumin solution; obtaining an albumin nanofiber membrane with high orientation degree through electrostatic spinning; sequentially treating with a double-bond modifier, a hydrophobic modifier and a sulfydryl RGD peptide solution to obtain a functionalized nanofiber membrane; and finally, covering albumin solution on the upper and lower surfaces and drying to form the anisotropic composite film with a three-layer structure. According to the preparation method, high-orientation arrangement and accurate biological activity modification of nanofibers are realized by regulating and controlling a spinning process and surface functional modification, and the prepared composite membrane has excellent anisotropic mechanical properties, controllable degradability and a cell guiding function, and is suitable for the biomedical fields of tissue repair, adhesion prevention, soft tissue reconstruction and the like.

The embodiment of the invention provides a pH-sensitive tumor targeting nanoparticle as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving baicalin in an organic solvent to obtain an organic phase; mixing the organic phase with an albumin solution to obtain baicalin albumin nanoparticles; dissolving folic acid in a buffer solution, adding a shrinking agent and a carboxyl activating agent, and reacting to obtain a folic acid activating solution; mixing the baicalin albumin nanoparticles with a folic acid activating solution, and reacting to obtain baicalin folic acid coupled albumin nanoparticles; the preparation method comprises the following steps: dissolving mPEG-NH2 and DCA in a buffer solution, and reacting to obtain mPEGA-DCA; adding a shrinking agent and a carboxyl activating agent into the mPEGA-DCA solution to obtain a pH sensitive polymer; and mixing the pH-sensitive polymer and the baicalin folic acid coupled albumin nanoparticles to prepare the pH-sensitive tumor targeting nanoparticles. According to the application, the toxic and side effects of the baicalin can be reduced from multiple aspects, and the tumor targeting capability of the baicalin is improved, so that the anti-tumor capability of the baicalin is enhanced.

The present invention relates to human albumin solutions and in particular to methods of preparation and uses thereof. The invention also relates to a human albumin solution having an increased mercaptoalbumin content and / or an increased transport capacity for reactive oxygen species, reactive nitrogen species and endogenous and exogenous ligands. Some embodiments of the invention have been particularly developed for the treatment of disease and will be described hereinafter with reference to the present application. However, it is understood that the present invention is not limited to this particular field of application.

The invention discloses a preparation method and application of Fe3O4 nanoparticles modified by an amyloid-like albumin coating, and relates to the technical field of nano-medicines. The preparation method comprises the following steps: S1, performing amyloid-like transformation on human serum albumin to prepare an amyloid-like albumin solution; and S2, modifying the surface of a Fe3O4 nanoparticle with the amyloid-like albumin solution prepared in the step S1 to prepare the nanoparticle modified by the amyloid-like albumin coating. According to the preparation method and the application of the Fe3O4 nano-particles modified by the amyloid-like albumin coating, the Fe3O4 nano-particles modified by the amyloid-like albumin coating, which have macrophage phagocytosis and inflammation bidirectional regulation and control effects, are innovatively constructed through an amyloid-like protein self-assembly technology.

The invention provides a preparation method of all-transretinoic acid and arsenic trioxide composite nano sustained-release microspheres, which comprises the following steps: a, adding albumin and L-reduced glutathione into deionized water, and carrying out reduction reaction on disulfide bonds in albumin molecules to obtain a reduced albumin aqueous solution; b, taking a sodium hydroxide solution of ATO, and then adding the sodium hydroxide solution of ATO into the reductive albumin aqueous solution obtained in the step a to obtain an ATO-albumin solution; c, ATRA is added into absolute ethyl alcohol and stirred, and an ethyl alcohol solution of ATRA is obtained; d, dropwise adding the ATRA ethanol solution obtained in the step c into the ATO-albumin solution obtained in the step b by using a micro-injection pump to obtain an ATRA and ATO composite nano sustained-release microsphere crude product; and e, centrifuging the crude product, cleaning with deionized water, and freeze-drying to obtain the microsphere product. The preparation method of the product is rapid, simple and convenient, the targeting property is strong, the effect of resisting tumor growth and metastasis of the medicine can be obviously enhanced, and the biological safety is good.