7 results about "Albumin solution" patented technology

A composition and method for perfusing tissue. A perfusate is composed of an endothelial cell stabilizer. thrombocyte concentrate (TC). The perfusate may further comprise a protein solution that may be an albumin-based solution or fresh frozen plasma (FFP). The perfusate composition may further comprise red blood cells. The perfusate composition may be perfused through an organ that has been removed from an organ donor and is being prepared for transport and transplant into a recipient. The perfusate composition may be perfused through an organ using a machine perfusion device.

The application belongs to the technical field of biological medicine material preparation, and particularly relates to albumin nanoparticles loaded with ferroptosis related drugs and a preparation method thereof. The ferroptosis related drug composition comprises porphyrin drugs, unsaturated fatty acid drugs-cystamine and sulfosuccinimidyl oleate (SSO). The porphyrin drugs, the unsaturated fatty acid drugs-cystamine and the SSO are slowly added dropwise into an albumin solution under stirring, the drugs enter the hydrophobic cavities of the albumin, and uniform nanoparticles are spontaneously formed. The obtained nanoparticles are small in size and uniform, can efficiently deliver drugs through passive targeting mediated by the EPR effect and active targeting mediated by the Gp60-Caveola-SPARC pathway, improve stability, reduce accumulation at non-target sites and systemic toxicity, amplify the effect of ferroptosis after combination compared with the ferroptosis related drugs used alone, and thus produce a synergistic anti-tumor effect.

This invention discloses a method for preparing flower-shaped silver powder. The method includes: 1. Preparing a silver nitrate solution to obtain solution A; 2. Preparing equal volumes of ascorbic acid solution and soy albumin solution, and mixing them to obtain solution B; 3. At room temperature, simultaneously and symmetrically adding solution A and anhydrous ethanol to solution B to react, allowing the generated silver particles to settle, be washed, and dried to obtain flower-shaped silver powder; applying this flower-shaped silver powder to low-temperature silver paste and Raman-enhanced substrates. The preparation method of this invention effectively controls the morphology of the reduced product silver powder through the synergistic effect of soy albumin and ethanol, preparing flower-shaped silver powder with a multi-level nanostructure. This flower-shaped silver powder has good dispersibility and high activity, and the preparation process is simple, efficient, environmentally friendly, and easy to scale up for production, making it suitable for fields such as electronics, catalysis, and biomedicine.

The application discloses a pH-responsive deformable double-targeting nano-protein and a preparation method and application thereof, and the method comprises the following steps: after carboxylated mannose is activated through an EDC / NHS system, the carboxylated mannose is reacted with an albumin solution, and mannose-modified albumin is obtained through dialysis purification; the mannose-modified albumin is mixed with an inhibitor IRG1-IN-1, and IRG1-IN-1 is loaded through hydrophobic interaction to obtain mannose albumin nanoparticles; ferritin is used as a carrier, and oxaliplatin is loaded through a solvent displacement method to obtain ferritin nanoparticles; CHO-PEG2k-CHO is used as a connecting agent, and the mannose albumin nanoparticles and the ferritin nanoparticles are crosslinked through a Schiff base reaction to form large-size pH-responsive double-targeting nano-proteins. The double-targeting separable characteristics of the nano-proteins can realize specific treatment, kill tumor cells, interfere with immune cell metabolism, block metabolic immunosuppression, and promote anti-tumor immunotherapy.

The application discloses a kind of albumin-paclitaxel twin drug nanoparticles and application, belong to the field of pharmaceutical chemistry and biopharmaceutical technology, specifically related to albumin is mixed with water, and albumin solution is prepared;Paclitaxel or paclitaxel twin drug compound is mixed with organic solvent, and drug solution is obtained;Albumin solution and drug solution are mixed, and after ultrasonic dispersion, spinning, ultrafiltration are carried out to prepare albumin binding type paclitaxel or albumin-paclitaxel twin drug nanoparticles.The albumin-paclitaxel twin drug prepared in the application can utilize the special GP60-cellar protein-SPARC transport mechanism of albumin, increase the accumulation of drug in tumor, and in vivo and in vitro experiments prove that the albumin-paclitaxel twin drug nanoparticles described in the application have good antitumor effect.

The invention discloses an anisotropic composite film based on albumin and a preparation method and application thereof, and belongs to the technical field of biomedical materials. The preparation method comprises the following steps: reducing an albumin solution by using a reducing agent and adjusting the pH value of the albumin solution by using organic alkali to obtain a denatured albumin solution; obtaining an albumin nanofiber membrane with high orientation degree through electrostatic spinning; sequentially treating with a double-bond modifier, a hydrophobic modifier and a sulfydryl RGD peptide solution to obtain a functionalized nanofiber membrane; and finally, covering albumin solution on the upper and lower surfaces and drying to form the anisotropic composite film with a three-layer structure. According to the preparation method, high-orientation arrangement and accurate biological activity modification of nanofibers are realized by regulating and controlling a spinning process and surface functional modification, and the prepared composite membrane has excellent anisotropic mechanical properties, controllable degradability and a cell guiding function, and is suitable for the biomedical fields of tissue repair, adhesion prevention, soft tissue reconstruction and the like.

The invention provides a preparation method of all-transretinoic acid and arsenic trioxide composite nano sustained-release microspheres, which comprises the following steps: a, adding albumin and L-reduced glutathione into deionized water, and carrying out reduction reaction on disulfide bonds in albumin molecules to obtain a reduced albumin aqueous solution; b, taking a sodium hydroxide solution of ATO, and then adding the sodium hydroxide solution of ATO into the reductive albumin aqueous solution obtained in the step a to obtain an ATO-albumin solution; c, ATRA is added into absolute ethyl alcohol and stirred, and an ethyl alcohol solution of ATRA is obtained; d, dropwise adding the ATRA ethanol solution obtained in the step c into the ATO-albumin solution obtained in the step b by using a micro-injection pump to obtain an ATRA and ATO composite nano sustained-release microsphere crude product; and e, centrifuging the crude product, cleaning with deionized water, and freeze-drying to obtain the microsphere product. The preparation method of the product is rapid, simple and convenient, the targeting property is strong, the effect of resisting tumor growth and metastasis of the medicine can be obviously enhanced, and the biological safety is good.