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Folic acid quantitative detection kit

A quantitative detection and kit technology, applied in biological testing, material inspection products, color/spectral characteristic measurement, etc., can solve the problems of weak anti-interference ability, poor repeatability of approval documents, low sensitivity, etc., and achieve reasonable production cost and specificity High, high-sensitivity effects

Inactive Publication Date: 2018-06-15
XINCHANG COUNTY MEIDI BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Each of the above detection methods has its disadvantages: for example, the microbial method has a long detection cycle, poor repeatability of approval documents, and cumbersome preservation of strains, which are generally used to detect the content of folic acid in food and dairy products; colorimetry, ultraviolet spectrophotometry and chromatography The method has weak anti-interference ability and low sensitivity, and is generally used to detect the content of folic acid in pure products, vitamin preparations or injections; chemiluminescence, fluorescence and chromatography are gradually used in clinical experiments due to their high sensitivity and good specificity. , but due to the need for expensive special equipment and the high cost, it has not been widely promoted

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0051] Preparation of reagents in the kit

[0052] 1. Preparation of folate-bound albumin

[0053] Step A: Prepare the stock solution

[0054] a1, prepare 4-hydroxyethylpiperazineethanesulfonic acid buffer solution with a pH value of 10; a2, prepare carrier protein solution: get 50 mg of carrier protein and add 4-hydroxyethylpiperazineethanesulfonic acid buffer solution with a pH value of 10 Obtain carrier protein solution in;

[0055] Step B: Weigh the analytical reagents of folic acid, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide sodium hydrochloride and N-hydroxysulfosuccinimide respectively;

[0056] Step C: Activation

[0057]c1. Add folic acid, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide sodium hydrochloride, and N-hydroxysulfosuccinimide in sequence in dimethylformamide with a pH value of 10 Analytical reagent, react at room temperature for 1 hour to obtain mixed solution A; c2, mix the carrier protein solution prepared in step a2 with mixed solution A obtained i...

Embodiment 2

[0075] Components of Example 2 Folic Acid Quantitative Detection Kit

[0076] Folic acid quantitative detection kit, including reagent R1, reagent R2, calibrator and quality control product; wherein:

[0077] (1) Reagent R1 includes the following components:

[0078]

[0079] (2) Reagent R2 includes the following components:

[0080] 3-(N-morpholino)-2-hydroxypropanesulfonic acid buffer pH7.2 50mM

[0081] Folic acid antibody latex particle suspension 1.2%

[0082] Sodium azide 1‰;

[0083] The packaging specifications of the reagents in the kit are reagent R1 60ml×1 and reagent R2 15ml×1. The kits should be stored at 2-8°C in the dark and sealed to avoid freezing and pollution. It can be stable for 12 months from the date of production. Please use up within 30 days after opening.

[0084] (3) Preparation of Calibrator and Quality Control

[0085] ① Accurately weigh 1 mg of folic acid chromatographically pure (Sigma) sample, add about 900 ml of purified water and stir...

Embodiment 3

[0092] The detection of folic acid in the embodiment 3 serum samples

[0093] according to figure 1 The parameter table shown sets the Olympus 2700 automatic biochemical analyzer, and the concentration and position of the standard are input according to the actual usage. Quantitative detection of folic acid concentration in the present embodiment can also use various automatic biochemical analyzers in the prior art, such as Hitachi 7080,7600; Olympus 400; Beckman CX9, LX20; Toshiba 40; DuPont RXL; Abbott C8000 and other automatic biochemical analyzers.

[0094] After the blood sample to be tested is collected, it is centrifuged, and the serum is taken for use; using the Olympus 2700 automatic biochemical analyzer, add 200 μL of reagent R1 to the blank tube, standard tube and measurement tube respectively, and pour into the measurement tube within 10 seconds. Add 10 μL of the serum to be tested, add 10 μL of distilled water to the blank tube, add 10 μL of calibrator to the st...

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Abstract

The invention provides a folic acid quantitative detection kit suitable for all kinds of fully automatic biochemical analyzer, wherein the kit includes a reagent R1, a reagent R2, a calibration product and a quality control product. The reagent R1 includes a folic acid binding albumin solution, and the reagent R2 includes a folic acid antibody latex particle suspension. The folic acid quantitativedetection kit can quickly quantitatively detect the content of folic acid in whole blood, erythrocytes and serum in ten minutes, has the advantages of high specificity, high sensitivity and simple operation. In addition, the kit has reasonable preparation cost, and can be used in batches in clinic.

Description

technical field [0001] The invention belongs to the field of medical detection kits, in particular to a folic acid quantitative detection kit suitable for various automatic biochemical analyzers. Background technique [0002] Folic acid, namely vitamin B11, is an essential growth element for the human body and has important physiological functions. One is to participate in various methylation reactions as a methyl donor in the body; the other is to participate in the synthesis of purine and thymine, that is, to synthesize ribonucleic acid (RNA) and deoxyribonucleic acid (DNA); the third is to participate in amino acid metabolism. The human body cannot synthesize folic acid itself and must be supplemented through exogenous sources. Lack of folic acid will cause megaloblastic anemia and leukopenia, especially if the intake of folic acid is insufficient in the first three months of pregnancy, the fetus will be prone to serious diseases such as congenital neural tube defects. ...

Claims

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Application Information

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IPC IPC(8): G01N33/82G01N21/31
Inventor 董静平
Owner XINCHANG COUNTY MEIDI BIOLOGICAL TECH
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