Thymidylate synthase preparation loaded albumin nano-microsphere and preparation method thereof
A nanotechnology of thymidylate synthase and albumin, which is applied in the direction of medical preparations containing active ingredients, drug combinations, organic active ingredients, etc., to achieve good dispersion, good slow-release effect, in vivo performance, and high solubility
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Embodiment 1
[0034] Example 1 Preparation of Loaded Pemetrexed Modified Bovine Serum Albumin Drug-loaded Nanoparticles
[0035] In a 4mL reactor, dissolve 25mg bovine serum albumin and 6.3mg dithiothreitol in 1mL distilled water, react at 37°C for 0.5h, add sodium hydroxide solution to adjust the pH of the system to 8.0, add dropwise 1.5mg / 1.5 mL pemetrexed acetone solution to form nano-microspheres, add 20 μL of 0.5 mg / mL calcium sulfate solution and react for 15 minutes, react at 60°C for 5 minutes, and rotate at 37°C for 15 minutes. The ultrafiltration centrifuge tube was centrifuged to obtain the aqueous phase dispersion of the modified bovine serum albumin drug-loaded nano-microspheres. Adding 2% mannitol of the total mass of the system to freeze-dry to obtain drug-loaded nano microsphere powder.
Embodiment 2
[0036] Example 2 Preparation of modified bovine serum albumin drug-loaded nanospheres loaded with raltitrexed
[0037]In a 4mL reactor, dissolve 15mg of bovine serum albumin and 4.8mg of dithiothreitol in 1mL of distilled water, react at 37°C for 0.5h, add hydrochloric acid solution to adjust the pH of the system to 5.0, and dropwise add 1.5mg / mL of threitol Tetrexed absolute ethanol solution 1.1mL, form nano-microspheres, add 20 μL of 0.5 mg / mL zinc sulfate solution and react for 15 minutes, add 0.02 mM calcium sulfate solution 100 μL, stir for 2 hours, 37 ° C rotary evaporation for 15 minutes, nano-microspheres Ultracentrifuge at 12,000 rpm for 3 minutes, and redissolve to obtain the aqueous phase dispersion of modified bovine serum albumin drug-loaded nano-microspheres. Adding 2% mannitol of the total mass of the system to freeze-dry to obtain drug-loaded nano microsphere powder.
Embodiment 3
[0038] Example 3 Preparation of modified bovine serum albumin drug-loaded nanospheres loaded with raltitrexed
[0039] In a 4mL reactor, dissolve 15mg of bovine serum albumin and 3.5mg of mercaptoethanol in 1mL of distilled water, react at 37°C for 0.1h, add sodium hydroxide solution to adjust the pH of the system to 9.0, and dropwise add 1.5mg / mL Ratidine Add 1.5 mL of anhydrous ethanol solution of Trexed to form nano-microspheres, add 20 μL of 0.25 mg / mL calcium chloride solution and react for 2 hours, then react at 70°C for 2 minutes, then evaporate at 37°C for 15 minutes, freeze and centrifuge the nano-spheres at 100,000 rpm After 5 minutes, redissolve to obtain the aqueous phase dispersion of modified bovine serum albumin drug-loaded nano-microspheres. Adding 5% of the total mass of glucose to the system is freeze-dried to obtain drug-loaded nano-microsphere powder.
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