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Bovine serum albumin extraction method and bovine serum albumin

A technology of bovine serum albumin and its extraction method, which is applied in the direction of serum albumin, albumin peptide, peptide preparation method, etc., and can solve problems such as limiting the application of bovine serum albumin

Inactive Publication Date: 2016-11-02
CHENGDU YUANRUI BIOTECH CO LTD
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

Such impurities limit the further application of bovine serum albumin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0047] The method for extracting bovine serum albumin provided by the present embodiment comprises the following steps:

[0048] 1. Blood Separation

[0049] Get 6000 milliliters of fresh bovine blood, add 667 milliliters of 38% trisodium citrate, make the final weight concentration of trisodium citrate in bovine blood be 3.8%, then pass through blood cell separator (GFX-105 blood separator, Liaoyang Xianglong Pharmaceutical Machinery Co., Ltd.) to separate bovine blood (rotating speed 16300rpm / min) to obtain blood cells and plasma.

[0050] 2. Dissolution of protein precipitate

[0051] 2.1 Take 4000 ml of plasma in step 1, add 6000 ml of saturated ammonium sulfate to make the final concentration of ammonium sulfate in the plasma 60%, and stir for 1 hour to precipitate the protein in the plasma, remove the supernatant, and retain the protein precipitate .

[0052] 2.2 Add 6000 ml of ultrapure water at a mass ratio of 5:1 to the protein precipitate to dissolve the above pro...

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PUM

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Abstract

The invention discloses a bovine serum albumin extraction method which comprises the following steps: first concentration and liquid exchange: conducting ultrafiltration concentration on a protein dissolution solution through a first ultrafiltration membrane to obtain a protein concentrate; first purification: conducting chromatographic purification collection on the protein concentrate through a weak anion exchange medium to obtain a crude albumin solution; second concentration and liquid exchange: conducting ultrafiltration concentration on the crude albumin solution through a second ultrafiltration membrane to obtain a crude albumin concentrate; second purification: conducting chromatographic purification collection on the crude albumin concentrate through a strong cation exchange medium to obtain an albumin solution. According to the extraction method, through the steps of two times of ultrafiltration concentration and two times of chromatographic purification, impurities in blood plasma can be removed to the maximum extent, and the purity of the bovine serum albumin is improved. Besides, the invention also provides bovine serum albumin obtained through the method.

Description

technical field [0001] The invention relates to the technical field of bovine serum albumin extraction, in particular to a bovine serum albumin extraction method and bovine serum albumin. Background technique [0002] At present, bovine serum albumin (BSA), also known as the fifth component, is a globulin in bovine serum, containing 583 amino acid residues, with a molecular weight of 66.43kDa and an isoelectric point of 4.7. It is a biochemical reagent widely used in various fields of medicine and pharmacy, and can also be used as medical and health food and condiment. [0003] There are many methods for preparing albumin in bovine serum, mainly by salting out, organic solvent precipitation and heat shock extraction. The salting-out method is obtained by ammonium sulfate fractional precipitation and refined by octanoic acid treatment; the organic solvent precipitation method was first used in the production of human blood products, using the low dielectric constant properti...

Claims

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Application Information

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IPC IPC(8): C07K14/765C07K1/36C07K1/34C07K1/30C07K1/18
CPCC07K14/765
Inventor 熊川杨汝春邓飞
Owner CHENGDU YUANRUI BIOTECH CO LTD
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