Method for forming albumen layer by mediating albumin on surface of material, biological material and application thereof

An albumin-mediated technology, applied in the field of biomaterials, can solve the problems that the biocompatibility of titanium and titanium alloys does not meet the clinical requirements

Active Publication Date: 2019-06-25
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, as a cardiovascular implant material for the treatment of coronary heart disease, the biocompatibility of titanium and titanium alloys has not yet met the clinical requirements.

Method used

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  • Method for forming albumen layer by mediating albumin on surface of material, biological material and application thereof
  • Method for forming albumen layer by mediating albumin on surface of material, biological material and application thereof
  • Method for forming albumen layer by mediating albumin on surface of material, biological material and application thereof

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Experimental program
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Effect test

Embodiment 1

[0050] This embodiment provides a method for mediating albumin to form a protein layer on the surface of a material, which includes the following steps:

[0051] (1) Alkali activation. After cleaning the surface of pure titanium, it is immersed in 2.5mol / L NaOH solution and reacted for 8 hours. Then, after ultrasonic cleaning with RO water, immerse in UP water, let stand at 80°C for 8 hours, ultrasonically clean with UP water, and then dry for use.

[0052] (2) Surface amination. Submerge the alkali-activated sample in a poly-L-lysine solution with a concentration of 2 mg / mL (poly-L-lysine with an average molecular weight of about 150 KDa is dissolved in a phosphate buffer with pH=7), and the reaction 8h, and then washed 3 times with phosphate buffered saline and stored for later use.

[0053] (3) Covalent immobilization of PAMAM. Use PAMAM with a molecular weight of about 1000 (carboxyl at the end) to configure a 0.5 mg / mL dendrimer solution, add carboxyl activator dichlo...

Embodiment 2

[0056] This embodiment provides a method for mediating albumin to form a protein layer on the surface of a material, which includes the following steps:

[0057] (1) Alkali activation. After cleaning the surface of pure titanium, it was immersed in a 3.5mol / L NaOH solution and reacted for 12 hours. Then, after ultrasonic cleaning with RO water, immerse in UP water, let stand at 90°C for 12 hours, ultrasonically clean with UP water, and dry for later use.

[0058] (2) Surface amination. Submerge the alkali-activated sample in a poly-L-lysine solution with a concentration of 3 mg / mL (poly-L-lysine with an average molecular weight of about 300 KDa is dissolved in a phosphate buffer solution of pH=8), and the reaction 12h, and then washed 3 times with phosphate buffered saline, and then stored for future use.

[0059] (3) Covalent immobilization of PAMAM. Use PAMAM with a molecular weight of about 100,000 (carboxyl at the end) to configure a 10 mg / mL dendrimer solution, add ca...

Embodiment 3

[0062] This embodiment provides a method for mediating albumin to form a protein layer on the surface of a material, which includes the following steps:

[0063] (1) Alkali activation. After cleaning the surface of pure titanium, it is immersed in 3mol / L NaOH solution and reacted for 10 hours. Then, after ultrasonic cleaning with RO water, immerse in UP water, let stand at 85° C. for 10 hours, ultrasonically clean with UP water, and then dry for use.

[0064] (2) Surface amination. Submerge the alkali-activated sample in a poly-L-lysine solution with a concentration of 2.5 mg / mL (poly-L-lysine with an average molecular weight of about 200 KDa is dissolved in a phosphate buffer with pH=7.4), React for 10 h, then wash with phosphate buffered saline for 3 times and store for future use.

[0065] (3) Covalent immobilization of PAMAM. Use PAMAM with a molecular weight of about 9000 (carboxyl at the end) to configure a 1 mg / mL dendrimer solution, add carboxyl activator N-hydroxy...

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Abstract

The invention discloses a method for forming an albumen layer by mediating albumin on the surface of a material, a biological material and application thereof and relates to the technical field of biomaterials. The method for forming an albumen layer by mediating albumin on the surface of a material includes standing a to-be-supported material in an alkaline solution and then obtaining an alkali-activated material; fixing dendrimers on the surface of the alkali-activated material, and then immersing the modified material in an albumin solution for standing reaction. Preferably, the dendrimer is a polyamide-amine dendrimer PAMAM; and more preferably, the molecular weight of PAMAM is 6000 to 10000. The biological material forms a PAMAM layer and the albumin layer on the surface of the to-be-supported material, and the albumin is mediated to form the albumen layer. The biological material is used for preparing a cardiovascular implant material and can reduce the adhesion of platelets to achieve anticoagulation, and the biocompatibility of the material is improved.

Description

technical field [0001] The invention relates to the technical field of biological materials, and in particular to a method for mediating albumin to form a protein layer on the material surface, biological materials and applications thereof. Background technique [0002] Due to their good biocompatibility, mechanical properties, corrosion resistance and machinability, medical titanium (Ti) and titanium alloys have become an important branch of materials science. Initially, pure titanium was first used in the United States and the United Kingdom to make biomedical materials such as screws, hip joints, and intramedullary nails. Since 1972, my country has begun to use domestic titanium and titanium alloy products, and many domestic hospitals have used titanium and titanium alloys successively. For clinical treatment and research. In the mid-1990s, titanium metal and its alloys were widely used in the fields of neurosurgery, oral and maxillofacial surgery, orthopedic surgery, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/10A61L31/14C07K14/765C07K1/113C07K17/08C07K17/14
Inventor 陈俊英彭行溉李莉曾峥魏来谭建英王焕然翁亚军黄楠
Owner SOUTHWEST JIAOTONG UNIV
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