The invention discloses a non-support three-dimensional printing method based on inclined layering. The method comprises five steps of model surface overhanging area identification, model reestablishment, horizontal slicing, motion code coordinate transformation, and path coupling; a path includes two parts: a horizontal layered path and an inclined layered path. A path track inclined relative to a horizontal plane is formed at an overhanging part; when the region is printed, a new layer of material can be guaranteed to be not only suffered from gravity but also is suffered from an adhesive force in a deposition process due to the presence of a formed printing layer on an upper layer. An additional supporting material is omitted, a printing raw material is saved, the printing efficiency is improved, and a process of subsequent support removal is omitted. The inclined layered three-dimensional printing path provided by the invention has a common code format, can be directly applied in commercial and open-source desktop type three-dimensional printers, has extensive application prospects, and can be extended to the three-dimensional printing technology in biology, chemical engineering, macromolecule and food industries.