Single-stranded DNA nucleic acid modified chitosan magnetic microsphere preparation method
A magnetic microsphere, nucleic acid modification technology, applied in chemical instruments and methods, other chemical processes, etc., to achieve good biocompatibility, reduce detection limit, and overcome the effect of easy shedding
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[0026] This embodiment takes adenosine as an example to describe the present invention in detail, but this does not limit the protection scope of the present invention.
[0027] Such as figure 1 As shown, the modified CS / Fe based on adenosine nucleic acid aptamer 3 o 4 The preparation method of magnetic microspheres is as follows:
[0028] (1) Accurately weigh 14.04g FeCl 3 ·6H 2 O, dissolve with boiled and cooled deionized water, transfer to a 500mL three-necked flask, wash the beaker with boiled and cooled deionized water and transfer all to the three-necked flask to obtain a brownish red solution, feed nitrogen for 5min, add 7.19g FeSO 4 ·7H 2 O mechanically stirred to dissolve. Transfer the three-neck flask to a 90°C water bath, add 40mL of 25% ammonia water drop by drop with a separatory funnel, black precipitate forms, and continue stirring at constant temperature for 1h. For magnetic separation, place the product on a magnet. After 5 minutes, the lower layer is a...
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