The application relates to the technical field of biological extraction, and discloses a
spleen polypeptide extract as well as a preparation method and application thereof. The preparation method takes fresh
spleen tissue as
raw material, and realizes preliminary separation of proteins, polypeptides and nucleic acids through an optimized
isoelectric point grading process after pretreatment, homogenization, repeated freeze-thaw crushing,
centrifugal separation and
ultrafiltration grading, and finally obtains high-content products by adopting targeted
chromatography purification technology. The
spleen polypeptide extract and related products can be widely applied to the fields of biological medicines (such as immunomodulators and blood substitutes), food additives (such as
protein fortifiers) and scientific
reagent fields, the process is mild and has good
repeatability, the comprehensive utilization of the spleen
raw material is realized, the product added value is improved, and the application prospect of industrialization is good.