Non-programmable-controlled cooling cryopreservation method and protection agent for hematopoietic stem cells at minus 80DEG C
A technology of hematopoietic stem cells and cryopreservation protective agent, which is applied in the field of cold storage of stem cells, can solve the problems of differences in stem cell preservation effect and storage time, large differences in the use of protective agents, and difficulty in ensuring the safety of stem cells, etc., to achieve cheap raw materials and simple operation , the effect of a simple formula
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[0018] 1. The consumption of each reagent of hematopoietic stem cell-80 ℃ non-programmed cooling cryopreservation protection agent of the present invention, as shown in Table 1:
[0019] Table 1
[0020]
[0021] 2. The preparation method of hematopoietic stem cells -80 ℃ non-programmed cooling cryopreservation agent
[0022] 1. Prepare reagent A:
[0023] Get 100% DMSO and hydroxyethyl starch sodium chloride solution respectively according to the proportioning of embodiment 1-5, mix, pack in the bag.
[0024] 2. Preparation of Reagent B:
[0025] Prepare 40ml of albumin according to the ratio of Example 1-5, set aside.
[0026] 3. Hematopoietic stem cells -80 ℃ non-programmed cooling method
[0027] Take a 500ml empty bag, extract 60ml of stem cells in the stem cell bag, inject it into the empty bag, keep the needle, inject 40ml of reagent B into the bag and mix well, take the reagent A just prepared (the volume of reagent A is equal to the volume of stem cells + reage...
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