Preparation method and using method of hydrophilic adhesive glass slide

A glass slide, hydrophilic technology, applied in analytical materials, instruments, etc., can solve problems such as uneven dyeing, and achieve the effect of overcoming uneven dyeing, easy to spread evenly, and good compatibility

Inactive Publication Date: 2015-04-08
沈洪武
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the shortcomings of uneven staining that often occurs when ordinary adhesive slides are used on automatic immunohistochemical instruments, and to provide a method for preparing hydrophilic adhesive slides and a method for using them

Method used

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Examples

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Comparison scheme
Effect test

preparation example Construction

[0021] A method for preparing a hydrophilic adhesive slide, comprising the following steps:

[0022] 1) Soak the cleaned glass slide in the aminosilane solution with a volume concentration of 0.8-8% or the polylysine solution with a volume concentration of 0.1-1%, soak for 1-10min;

[0023] 2) Take out the above-mentioned slides, wash them with water, and dry them to obtain the adherent slides;

[0024] 3) Soak the above-mentioned adhesive slides in albumin solution with a mass concentration of 0.5-5% for 1-10 minutes;

[0025] 4) Take out the glass slide soaked in the albumin solution, wash it with water, and dry it to obtain a hydrophilic adhesive glass slide.

[0026] The solvent of the aminosilane solution is absolute ethanol, methanol or water.

[0027] The using method of described hydrophilic adhesion slide, comprises the following steps:

[0028] 1) After the tissue to be tested is sliced ​​into 3-5 microns, it is mounted on a hydrophilic adhesive glass slide;

[0...

Embodiment 1

[0032] A method for preparing a hydrophilic adhesive slide, comprising the following steps:

[0033] 1) Soak the cleaned glass slide in 2% aminosilane absolute ethanol solution (2mL aminosilane is dissolved in 98mL absolute ethanol) for 10min;

[0034] 2) Take out the above-mentioned slides, wash them with water (soak in water), and dry them to obtain the adherent slides;

[0035] 3) Soak the above-mentioned adhesive slides in albumin solution with a mass concentration of 1% for 10 minutes;

[0036] 4) Take out the glass slide soaked in the albumin solution, wash with water (soak in water), and dry to obtain a hydrophilic adhesive glass slide.

Embodiment 2

[0038] A method for preparing a hydrophilic adhesive slide, comprising the following steps:

[0039] 1) Soak the cleaned glass slide in 0.8% aminosilane absolute ethanol solution (0.8mL aminosilane is dissolved in 99.2mL absolute ethanol) for 1 min;

[0040] 2) Take out the above-mentioned slides, wash them with water (soak in water), and dry them to obtain the adherent slides;

[0041] 3) Soak the above-mentioned adhesive slides in albumin solution with a mass concentration of 0.5% for 1 min;

[0042] 4) Take out the glass slide soaked in the albumin solution, wash with water (soak in water), and dry to obtain a hydrophilic adhesive glass slide.

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PUM

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Abstract

The invention discloses a preparation method and a using method of a hydrophilic adhesive glass slide. The preparation method comprises the following steps: pre-processing the glass slide by using amino silane solutions with the volume concentration of 0.8-8 percent or polylysine solutions with the volume concentration of 0.1-1 percent; washing; airing; soaking the aired glass slide into albumin solutions with the mass concentration of 0.5-5 percent; taking out; washing; and airing to obtain the hydrophilic adhesive glass slide. The hydrophilic adhesive glass slide has hydrophilic groups on the surface, and is compatible with biological macromolecules and high in adhesion property; waterborne reagents are easily and uniformly spread on the surface of the glass slide, so that the defect that a common adhesive glass slide is always unevenly stained when used on an immunohistochemistry autostainer are overcome.

Description

technical field [0001] The invention relates to the field of glass slides, in particular to a method for preparing a hydrophilic adhesive glass slide and a method for using the same. Background technique [0002] At present, the ordinary adhesive slides made in China are hydrophobic adhesive slides. This hydrophobic adhesive slide often suffers from the disadvantage of uneven staining when used on Roche automated immunohistochemistry instruments. [0003] The liquid cover film of the Roche automatic immunohistochemistry instrument is light oil, which can adhere to the hydrophobic adhesive slide, resulting in uneven staining and unstained white flakes in the tissue to be tested, resulting in the failure of the tissue test. Therefore, hydrophobic adhesive slides cannot be used on Roche automated immunohistochemistry instruments. The Roche automatic immunohistochemical instrument is currently the most widely used automatic immunohistochemical instrument, accounting for about ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N35/00
Inventor 沈洪武
Owner 沈洪武
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