46 results about "Genus Trionyx" patented technology

The invention discloses an expanded pellet feed for the trionyx sinensis. The curing degree of the expanded pellet feed is 100 percent. The expanded pellet feed is prepared from domestic white fish meal, America white fish meal, imported eel-grade red fish meat, flour, cassava pieces, soy beans, non-transgenic soybean meal, fermented soybean meal, pregelatinization starch, milk powders, mineral premix, vitamin premix, monocalcium phosphate, calcium propionate and phosphatide oil by using a twin-screw extruding fully-curing expansion machine to firstly carry out quenching and tempering on the materials for 160 to 200 seconds under the conditions that a vapour pressure is between 0.4 and 0.5MPa, a humidity is between 19 and 23 percent, and a temperature is between 85 and 90 DEG C, and then carry out curing on obtained product under the conditions that a curing temperature is 180 DEG C, a cavity pressure is between 22 and 28atm and a curing time is between 25 and 30 seconds. Each kilogram of mineral premix comprises Mg, Na, K, Fe, Cu, Mn, Zn, Se, Co and I. The expanded pellet feed for the trionyx sinensis has the advantages of reasonable configuration of various elements required by nutrition and growth, complete curing, resistance to cause damage to all nutritional factors, low cost, higher stability in water and higher digestibility.

The invention discloses microsatellite DNA markers for Trionyx sinensis, which is characterized in that an enriched library of microsatellites (CA)n, (AAC)n, (ACAG)n and (GATA)n from the Trionyx sinensis is constructed, and positive clones of microsatellite sequences are screened and sequenced to obtain 63 clones containing microsatellite repetitive sequences, thus determining 15 microsatellite markers with rich polymorphism, namely, PSW01, PSW02, PSW03, PSW04, PSW05, PSW06, PSW07, PSW08, PSW09, PSW10, PSW11, PSW12, PSW13, PSW14 and PSW15. The microsatellite DNA markers for the Trionyx sinensis of the invention can be applied to researching genetic diversity and paternity test for the Trionyx sinensis in different geographical populations, have the advantage of good repeatability, and are reliable and effective molecular markers.

The invention provides a special feed for breeding trionyx sinensis and a preparation method of the special feed. The special feed is prepared from the following raw materials in parts by weight: 19-21 parts of fish meal, 4.5-5.5 parts of barley pest powder, 9-11 parts of shrimp meal, 10-12 parts of tubifex powder, 9-11 parts of soybean cake powder, 9-11 parts of wheat feed, 9-11 parts of corn flour, 4.5-5.5 parts of animal blood, 8-10 parts of wheat flour, 1 part of fish oil, 1 part of corn oil, 2.5-3.5 parts of bone meal, 0.3 part of lysine, 0.2 part of methionine, 0.5 part of salt, 2 parts of multivitamins, 1.8-2.2 parts of yeast extract powder, 0.5 part of probiotics and 0.5 part of mineral substances. The feed is scientific and reasonable in nutrient substances, and good in palatability, digestive absorption is facilitated, and the immunity of the trionyx sinensis is improved. Compared with the conventional feed, the yield of a control group of the product is improved by 4.9-6.5%, and the cost is reduced by 4.7-5.3%. The feed is simple in preparation technology, easy to operate, stable in product quality, and small in equipment investment, and has good economic benefits, social benefits and ecological benefits.

The invention provides a tortoise or trionyx sinensis spawning pond, which comprises a pond body, wherein the pond body is provided with a slope, a spawning table and a spawning groove, wherein the spawning table is provided with an installation groove; the spawning groove is arranged in the installation groove; the slope is arranged in the middle of a pond bottom; one end of the spawning table is connected with the slope; the other end of the spawning table is connected onto the pond wall; the spawning groove comprises a base plate and a three-face coaming arranged on the base plate; the spawning groove is provided with a filter screen layer and spawning sand covering the spawning groove; so that external influence on a tortoise or trionyx sinensis spawning process is low, the spawning sand is easy to clear, and the egg masses are easy to collect; after being taken out from the spawning groove, the egg masses are placed into cultivation water; and the spawning pond can be used as a tortoise or trionyx sinensis cultivation pond. The tortoise or trionyx sinensis spawning pond is simple in structure and is easy to realize.

The invention relates to a bionic mixed culture method of Trionyx sinensis and full-male Pseudobagrus fulvidraco. The method comprises the following steps of: fish pond selection and disinfection, fish seed and turtle seed putting, fish seed and turtle feed feeding, water quality regulation and alternate harvesting and putting. According to the invention, the traditional situation that the mixed culture of four household fish and turtle is replaced, full-male Pseudobagrus fulvidraco with high growth speed is selected, the culture density and specification are reasonably determined, and the technical operation instruction of culture is established, thus the problem that the comparison benefit decreases when turtle is cultured in the pond is solved, the culture risk is reduced, the water utilization factor of the pond is simultaneously increased, and high-yield, high-efficiency, ecological and healthy mixed culture of Trionyx sinensis and full-male Pseudobagrus fulvidraco is realized.

The invention discloses a compound pellet feed with low fish meal dosage for trionyx sinensis juveniles and a preparation method thereof, and belongs to the field of feeds. The feed is prepared from the following raw materials in percentage by weight: 35-40 percent of fish meal, 10-15 percent of fermented soybean meal, 10-15 percent of soybean protein concentrate, 2-3 percent of corn gluten meal, 20-22 percent of gelatinized starch, 6-12 percent of compound animal-derived nutritional attractant, 1.5-2.0 percent of mixed fat, 0.1-0.3 percent of coated methionine, 0.1-0.3 percent of choline chloride, 0.8-1.2 percent of monocalcium phosphate, 0.3-0.5 percent of vitamin premix, 0.3-0.5 percent of mineral premix and 0.01-0.02 percent of phytase. The feed is comprehensive and balanced in nutrition, and can meet the nutritional requirement for the trionyx sinensis juveniles. Compared with current trionyx sinensis juvenile commercial feeds sold in the market, the compound pellet feed has the advantages that the dosage of the fish meal is reduced, the production cost is greatly reduced, the feeding effect is good, and the feed coefficient is low.

The invention discloses a trionyx feed, comprising the following raw materials: 35-50 parts of imported fish meal, 18-22 parts of starch, 6-10 parts of soy protein, 15-20 parts of soya bean meal, 1-3 parts of fish oil, 1-2 parts of monocalcium phosphate, 1-3 parts of adhesive, 2-5 parts of beer yeast, 0.5-2 parts of multi-vitamin, 0.1-1 part of sodium salt and 0.5-2 parts of mineral substance in parts by weight. The antibiotic-free trionyx feed of the invention has comprehensive nutritional ingredients and strong palatability and can take the effect of preventing dysplasia and anorexia of trionyx.

The invention discloses a feed capable of preventing and curing diseases of young trionyx sinensis and a preparation method thereof. The feed comprises the following raw materials: pork powder, shrimp meal, purple common perilla seed cake, sorghum, wheat bran, vinegar dregs, molasses, grape pomace, peach gum, tomatoes, pineapples, satsuma mandarin, soy sauce powder, onion powder, acetate ester starch, Chinese white cabbages, water spinach, mung bean sprouts, leek flowers, radix isatidis, citrus reticulata blanco peels, coptis roots, drying rehmannia roots, Chinese yams and rhubarb. The feed can be used for solving the problems of shortage of nutrition, rough preparation, poor palatability, low growth speed of the young trionyx sinensis, poor physique, easiness in illness formation, low survival rate and the like of the conventional feed for the young trionyx sinensis, the feed is rich in nutrition, fine in taste and faint scent in mouthfeel, can promote the growth and weight increment of infant terrapin and reduce the growth time, is capable of preventing and curing diseases and reducing the infection possibility of bacteria and parasite to the young trionyx sinensis, and has the advantages that the young trionyx sinensis has strong immunity, cannot get ill, and is high in survival rate, the economical benefits of the cultivation of the young trionyx sinensis can be greatly increased, and the promotion value is extremely high.

The invention discloses nutrient feed for adult turtles. The nutrient feed comprises the following raw materials in parts by weight: 20-25 parts of fish powder, 25-29 parts of maelworms, 14-20 parts of bean pulp, 22-25 parts of starches, 2-3 parts of brown sugar, 3-5 parts of corn oil, 0.5-1 part of dandelion, 0.5-1 part of ecliptae herba, 0.5-1 part of pumpkin seeds, 0.5-0.8 part of composite vitamins, and 0.4-0.6 part of composite minerals. The proportion of plant egg white to animal egg white in the nutrient feed is about 1:6, and 3%-5% of the corn oil is contained, so that the food-calling effect and the raising effect of the nutrient feed are optimum; a few edible traditional Chinese medicine materials are added, so that the immunity of the adult turtles can be increased, and the attack rate is reduced.

The present invention discloses a temperature and humidity control method of trionyx sinensis incubation room. The temperature and humidity control method comprises the following control steps: (1) providing a heating apparatus, a humidifying apparatus and a ventilation apparatus in the incubation room, and providing a water sprayiing apparatus at the roof of the incubation room; and (2) by virtue of a signal collected by the temperature sensor and humidity sensor provided in the incubation room, controlling the temperature of the incubation room at 28-30 DEG C and the humidity at 78%-85% by controlling the heating apparatus, the humidifying apparatus, the ventilation apparatus and the water spraying apparatus. According to the temperature and humidity control method of trionyx sinensis incubation room disclosed by the invention, after trionyx sinensis eggs in a trionyx sinensis pond are collected in a unified mode, the method is utilized to perform incubation; and the method has characteristics of a high incubation rate and short incubation time.

The invention provides a horizontal spiral ribbon mixer and a technology for processing of trionyx sinensis feed by the same. The horizontal spiral ribbon mixer comprises a base and a cylindrical mixing barrel fixed on the base. A main shaft runs through the mixing barrel along the axis, one end of the main shaft is dynamically connected to a motor fixed on the base through a speed reducer, the other end of the main shaft is rotatably connected to the base through a bearing, a plurality of support rods are welded and fixedly connected to the main shaft, the support rods are arranged on the horizontal main shaft along the axis direction at an equal interval, the upper and lower support rods are in staggered arrangement, and internal spiral ribbons and external spiral ribbons are welded and fixedly connected to the support rods. The technology for processing of trionyx sinensis feed by the horizontal spiral ribbon mixer specifically includes the steps of: artificial feeding, raw material cleaning, preliminary mixing, ultrafine grinding, secondary mixing, and finished product packaging. The horizontal spiral ribbon mixer provided by the invention improves the material mixing efficiency. And the technology improves the feed uniformity and reduces the feed doping rate.

The invention discloses a formula of a trionyx sinensis skin fester disease treating traditional Chinese medicine. The trionyx sinensis skin fester disease treating traditional Chinese medicine comprises licorice root, rhus chinensis mill, garlic, honeysuckle, wild chrysanthemum, rheum palmatum, ash bark, herba houttuyniae, hawthorn, coptis chinensis, scutellaria baicalensis, cortex phellodendri, isatis tinctoria, alisma plantago-aquatica linn, angelica, seasoned orange peel, radix asparagi, forsythia suspensa, fructus chebulae, artemisia carvifolia, smoked plum, pangolin, fortune paulownia flower, cayratia japonica (thunb.) gagnep, solanum lyratum thunb, viola philippica and snakegourd root. The trionyx sinensis skin fester disease treating traditional Chinese medicine of the present invention can be used for treating skin fester diseases, and has characteristics of obvious treatment effect and no side effect, wherein although the garlic and other heavy flavor materials are used, other drugs are subjected to reasonable compatibility, such that the purposes of flavor removing and treatment effect remaining are achieved.

An artificial hibemation method for storage of living tortoise or turtle for 15-30 days includes such steps as not applying feed for 1-2 days, capturing the living tortoises or turtles, cleaning them in clean water for 72 hr, individually packing them in boxes (one in one), storing at -5í¬-18 deg.C and immersing in clean water at 15-18 deg.C for 10 min every day.

The present invention discloses an instant five-spice trionyx sinensis and a preparation method thereof. The raw materials comprise trionyx sinensis, c. m. var. sarcodactylis flesh, loquat flesh, momordica. charantia var. abbreviata ser flesh, an onion juice, an apple juice, fresh lotus leaf, saururus chinensis, hibiscus mutabilis, thesium chinense turcz, rheum palmatum, hot pepper powder, oyster sauce, five-spice powder, white spirit, gravy salt, peanut oil and the like. The instant five-spice trionyx sinensis has beneficial effects of delicious taste, rich nutrition, easy eat and food therapy health effect, wherein a variety of Chinese herbs are matched so as to provide effects of yin nourishing, kidney invigorating, metabolism promoting, and life prolonging.

The invention relates to an anti-trionyx sinensis immune globulin monoclonal antibody, preparing the materials according to the following steps: (1) trionyx sinensis specificity antigen: collecting trionyx sinensis disposed in a bottle, condensing at a room temperature, standing the trionyx sinensis for 1.5-3h at 35-38 DEG C, standing for 8-12h at 3-6 DEG C again, centrifugating, taking supernatant, preparing a chromatographic column, crudely extruding ammonium sulfate water solution and diluting after dialyzing and concentrating, loading after filtering and eluting, wherein the firstly eluted protein IgM is the trionyx sinensis specificity antigen; (2) polyclonal antibody: sampling blood of the antibody immune animal, separating antiserum and purifying to obtain the polyclonal antibody; (3) hybridoma cell line: fusing myeloma cell with the splenocyte for generating the anti-trionyx sinensis immune globulin monoclonal antibody to obtain the hybridoma cell line; and (4) monoclonal antibody of anti-trionyx sinensis immune globulin: generating the monoclonal antibody by the hybridoma cell line. The trionyx sinensis immune serum titer is detected by the monoclonal antibody. The invention is suitable for researching turtle etiology and immunology.

The invention relates to the field of aquaculture, in particular to a takifugu obscures, coilia nasus schlegel and trionyx sinensis three-dimensional ecological breeding method, which comprises sevensteps of pond preparation, stocking of coilia nasus schlegel fingerlings and takifugu obscures, stocking of pseudorasbora parva and silver carps, bait feeding, daily management, and net pulling for catching. The invention fully utilizes the water space of a takifugu obscurus parent breeding pond, increases yield of the coilia nasus schlegel and trionyx sinensis under the conditions of not increasing the input, not reducing the takifugu obscures parent breeding density, and not affecting the takifugu obscurus parent gonad development, improves the water utilization rate of the pond, reduces theproduction cost, improves the economic benefit, reduces the feed cost, improves the quality of the trionyx sinensis, and makes the water quality of the pond more stable; the method of the invention is easy to operate and can be easily accepted and promoted.

The invention discloses a technical method for rapidly constructing a multiplex PCR system of more than 10 * of Trionyx sinensis by using public data. the method is mainly characterized in that: 1) using a public database to obtain a genome sequence of Trionyx sinensis; 2) analyzing the genome sequence of Trionyx sinensis by using MISA software, designing primers in batches by using Primer 3, andcalculating the compatibility of polymorphic site primers by using AutoDimer software; 3) constructing a multiplex PCR system while reserving the 10-12 locus with the strong compatibility of primer sequence; according to the concentration of 0.4 mu M of upstream primer and 10 mu M of downstream primer of each locus, preparing the primer premix, and then preparing the working solution of multiplexPCR primer by mixing the premix of all loci in the same volume; and 4) amplifying that multiplex PCR system by a conventional PCR method and genotyping the amplification product by capillary electrophoresis. The multiplex PCR system constructed by the invention has the advantages of high mark flux, accurate genotyping result and high speed, and can provide a molecular tool for the research work ofthe genetics and breeding of the soft-shelled turtle of China and the like.

The invention discloses feed for trionyx sinensis and a preparation method thereof. The feed for the trionyx sinensis is prepared from the following raw materials in parts by weight: 20-30 parts of shell powder, 1-3 parts of compound amino acid, 30-50 parts of corn flour, 8-10 parts of vegetable oil, 30-50 parts of cottonseed meal, 2-4 parts of multi-vitamin, 20-40 parts of shrimp meal, 10-12 parts of egg shells, 8-10 parts of nutrition adhesive, 10-12 parts of porkburger and 40-60 parts of water. The prepared feed for the trionyx sinensis is scientific and reasonable in formula, balanced in energy and protein and high in feed conversion ratio and can promote growth of the trionyx sinensis.

The invention discloses a gender proportioning method of a parent trionyx sinensis pool. The method comprises the following steps: (1) in the mating and spawning seasons of trionyx sinensis, selecting healthy parent trionyx sinensis with strong vitality from a male adult trionyx sinensis pool and a female adult trionyx sinensis pool; and (2) putting the selected parent trionyx sinensis with different genders in proportion in the parent trionyx sinensis pool to mat and spawn, wherein the proportion ratio of the male parent trionyx sinensis and the female parent trionyx sinensis is (3-4): 1. After the male and female parent trionyx sinensis which are proportioned according to a gender proportioning method of the parent trionyx sinensis, disclosed by the invention are put in the parent trionyx sinensis pool, the output eggs have the characteristics of high fertility rate and high hatchability.

An acoustic-sonic visual cage for catching of the Chinese softshell turtle is characterized in that the front end of a cage body is provided with a turtle entry, reverse steel wires which extend inward are connected to the turtle entry, a bait bag and a water level viewer are separately disposed in the middle of the cage body, the bait bag is fixed through stay ropes, a video lens, an observation lamp and a turtle ultrasonic apparatus are disposed in inner cavities of the water level viewer, respectively, a video transmission storage is connected to the upper end of the video lens, a video signal line is connected to the video transmission storage, and a plug is connected to the video signal line and is abutted to an interface of a computer.

The invention relates to a rice field farming method for continuously providing fresh bait for trionyx sinensis. The rice field farming method for continuously providing the fresh bait for the trionyxsinensis comprises the following specific steps that a rice field with convenient transportation, sufficient water source, no pollution, convenient drainage and irrigation, water and fertilizer retention, deep and waterproof plow layer is selected, periphery of the field after deep plowing is compacted, heightened by 50-60 cm and widened by 40-50 cm, an anti-escaping facility is built in the periphery by a color armor plate, and the selection and cultivation of rice varieties, the stocking of brood fishes and the stocking of the trionyx sinensis are carried out; one or two pond inspection areconducted every day, growth situations of the rice, the fish and the trionyx sinensis are inspected, the water quality and water level situations are inspected, and whether the anti-escaping facilityis strong or not is inspected. The rice field farming method for continuously providing the fresh bait for the trionyx sinensis scientifically utilizes the food chain relationship between species anda rice field farming method for continuously providing the fresh bait for the trionyx sinensis by using Tilapia mossambica which has the characteristic of iteroparity in a year is provided to improvethe comprehensive farming efficiency of the rice field, reduce the breeding cost and raise breeding quality of the trionyx sinensis.

The invention relates to fodder special for adult trionyx steindachneri. The nutritional fodder is prepared from the following components in parts by weight: 40 to 60 parts of fish powder, 12 to 18 parts of soybean powder, 4 to 8 parts of beef liver powder, 2 to 7 parts of yeast powder, 2 to 6 parts of pregelatinized starch, 1 to 6 parts of lysine, 1 to 3 parts of egg yolk powder, 2 to 5 parts of decavitamin, 1 to 6 parts or cooked carrot, 3 to 7 parts of brown sugar, 2 to 6 parts of fructus crataegi powder, 1 to 5 parts of vegetable oil and 15 to 18 parts of water. By adding the components such as the fish powder, the soybean powder, the beef liver powder, the lysine, the fructus crataegi powder and the vegetable oil, the fodder prepared by the invention is high in nutritional components; when the fodder prepared by the invention is applied for feeding trionyx seindachneri at regular intervals, the nutritional components obtained by the trionyx steindachneri are balanced, the growth speed of the trionyx steindachneri is fast, and the cultivation effect of the trionyx steindachneri is good.

The invention discloses a chicken embryo cultivation method of Trionyx sinensis systemic sepsis spherical virus. The method is characterized in that an SPF chicken embryo is used as an in vitro culture system of Trionyx sinensis systemic sepsis spherical virus; a healthy chicken embryo which has been pre-cultured for 4 to 6 days is selected; a pre-prepared Trionyx sinensis systemic sepsis spherical virus inoculation fluid is innoculated to the chick embryo yolk sac; cultivation is continued until the death of the chick embryo; and the dead chick embryo is collected to carry out homogenate centrifugal separation and obtain a supernatant containing Trionyx sinensis systemic sepsis spherical virus. It is the first time to apply chick embryo culture technique into the cultivation of Trionyx sinensis systemic sepsis spherical virus. In addition, the method can accomplish fast and efficient in vitro subculturing of Trionyx sinensis systemic sepsis spherical virus and is stable and convenient.