The invention provides a preparation method of nest soil capable of improving pseudobagrus fulvidraco egg hatchability. The nest soil is prepared from, by weight, 25 parts of modified starch, 46 parts of kaolin, 17 parts of porcellanite, 16 parts of talcum powder, 16 parts of ochre, 4 parts of gypsum rubrum, 12 parts of dried tangerine or orange peel, 3 parts of beautiful millettia roots, 7 parts of fructus forsythiae, 8 parts of pericarpium granati, 9 parts of the fruits of Chinese wolfberry, 1.5 parts of dandelion, 4 parts of polypodiaceae Pyrrosia lingua, 4 parts of asclepias curassavica, 3 parts of black sesame, 0.8 part of radix campanumoeae, 4 parts of water chestnuts, 0.3 part of eel bone, 14 parts of broom cypress, 5 parts of white quartz, 3 parts of geranium wilfordii, 4 parts of glutinous rice roots, 8 parts of isatis roots, 3 parts of cocklebur fruits, 3 parts of Chinese brake herb and 7 parts of banana peel. The nest soil is used for caving and breeding of pseudobagrus fulvidraco in the reproduction stage, nest soil ingredients are reasonable in matching, the materials are easy to obtain, the preparation method is simple, and the nest soil can improve the quality of water in a nest, promote pseudobagrus fulvidraco egg metabolism, improve the pseudobagrus fulvidraco egg hatchability, improve the resistance of pseudobagrus fulvidraco fry, and improve the anti-stress ability.