32 results about "Pseudobagrus fulvidraco" patented technology

The invention relates to the technical field of aquaculture feedstuff, in particular to feedstuff additive premix material. The feedstuff additive comprises the following components according to the following weight portion: 150 to 450 portions of astaxanthin, 300 to 700 portions of dunaliella sallina, 150 to 400 portions of vitamin E, 300 to 600 portions of Chinese thorowax, 100 to 250 portions of canthaxanthin, 300 to 600 portions of antioxidant, and 150 to 1,000 portions of corn protein powder. The feedstuff additive has no innocuous and side residue, no drug resistance, environmental protection, and no adverse effect on the feedstuff palatability. The feedstuff additive can obviously improve the body color of fishes in the yellow and red color systems such as golden pompano, pseudobagrus fulvidraco, clarias fuscus, sea bream, large yellow croaker, and the like, can make the body color display natural yellow or red color, and can effectively improve the immunity, reproductive capacity and meat quality of cultivated fish. In addition, the feedstuff additive has stable raw material sources and a simple manufacturing process.

The invention relates to a pseudobagrus fulvidraco puffed formulated feedstuff which is a mixture prepared from materials by using a puffed particle feedstuff processing method. The feedstuff is prepared from raw materials of, by weight: 30-40 parts of fish meal, 20-35 parts of soybean meal, 20-25 parts of flour, 5-10 parts of meat and bone meal, 2-6 parts of maize protein powder, 2-4 parts of soybean oil or fish oil, 1-2 parts of calcium dihydrogen phosphate, 0.8-1 part of potassium salt, 0.8-1 part of sodium salt, 0.8-1 parts of multivitamin, and 0.3-0.8 parts of a plant extract additive. The feedstuff provided by the invention is advantaged in scientific formulation, balanced and comprehensive nutrition, good stability in water, high feeding attraction activity, low feed coefficient, and low pollution to aquaculture water. With the added plant extract additive, the body color of artificially cultivated pseudobagrus fulvidraco can be substantially improved, and the body color of the artificially cultivated pseudobagrus fulvidraco is close to that of wild pseudobagrus fulvidraco. Therefore, the commodity value of the artificially cultivated pseudobagrus fulvidraco can be increased.

The invention relates to the technical field of breeding of rice field eels, in particular to a high-efficiency ecological three-dimensional breeding technology. The high-efficiency ecological three-dimensional breeding technology mainly comprises a set of complete ecological three-dimensional breeding mode for the rice field eels. The technology comprises contents such as site selection, net cage arrangement, aquatic plant planting and matching of fish species, wherein the matching of fish species is the core; a pool of about 10 mu is selected; micro-pores are arranged for oxygenation; knotless polythene net sheets are adopted by net cages; the area of each net cage is 6 m<2>, the depth is 1 m, and the depth of the part of the net cage immersed in the water is 0.5 m; the net cages are fixed by using brackets and are arranged in a straight line at an interval of 1 m and a row spacing of 2 m; the total area of the net cages does not exceed 50 percent of the total area; alternanthera philoxeroides are planted in 50-60 percent of the area of the net cages; after water is sterilized for 15 days, 5,000 pseudobagrus fulvidracos, each of which has the weight of 10 g, 1,000-2,000 loaches, each of which has the weight of 3-5 g, 100-500 silver carps, each of which has the weight of 100-500 g and 50-200 spotted silver carps, each of which has the weight of 50-500 g are bred per mu; 2 kg / m<2> of eels, each of which has the weight of 20-50 g, 5 loaches / m<2> and 1-2 toads, each of which has the weight of 10-100 g are bred in each cage; the eels seeds are soaked with 2 percent salt solution for 10-20 minutes before being put in the cages so as to prevent the occurrence of saprolegniasis.

The invention discloses a preparation method of a ricefield eel aldo-keto reductase polyclonal antibody. The preparation method comprises the following steps of: (1) cloning of ricefield eel Eakr gene; (2) establishment of a recombinant expression vector of the ricefield eel Eakr gene; (3) inducible expression and purification of recombinant protein of ricefield eel Eakr; and (4) preparation and Western blot analysis of polyclonal antibody. In the invention, a recombinant expression vector of ricefield eel Eakr gene is established by molecular biology and genetic engineering to induce expression, and purified recombinant expression protein is obtained through affinity chromatography. The ricefield eel Eakr polyclonal antibody prepared by the method disclosed by the invention can meet the experimental requirements of immunohistochemistry and Western blot, realizes in-vitro immunoassay, studies the functions of ricefield eel Eakr as well as ricefield eel immunofluorescent staining laser confocal microscopic observation and also can be applied to the immunodetection of aldo-keto reductase of grass carp, pseudobagrus fulvidraco, megalobrama amblycephala, snakehead and crucian.

The invention discloses a production method of triploid pseudobagrus fulvidraco, which belongs to the fish culture technology field. Artificial insemination is performed to the pseudobagrus fulvidraco female parent and male parent which are obtained through artificial induced spawning, after 10 to 15 minutes, triploid pseudobagrus fulvidraco is obtained through induction, and the duration time for induction is 10 to 20 minutes. After the triploid pseudobagrus fulvidraco is obtained through induction, the multiple of the chromosome is measured through the technologies, such as the karyotype analysis of chromosome, the flow cytometer, etc., and the triploid pseudobagrus fulvidraco is identified and sieved for culture. The method restrains the releasing time of the second polar body through time control on the artificial insemination, the induction through the physical or chemical method, and control on the induction duration time, and thereby the inductivity and the survival rate of the triploid pseudobagrus fulvidraco are greatly increased, and according to the experiment statistics, the artificial inductivity of the achieved triploid pseudobagrus fulvidraco is more than 50 percent, and the survival rate is more than 80 percent.

The invention relates to a bionic mixed culture method of Trionyx sinensis and full-male Pseudobagrus fulvidraco. The method comprises the following steps of: fish pond selection and disinfection, fish seed and turtle seed putting, fish seed and turtle feed feeding, water quality regulation and alternate harvesting and putting. According to the invention, the traditional situation that the mixed culture of four household fish and turtle is replaced, full-male Pseudobagrus fulvidraco with high growth speed is selected, the culture density and specification are reasonably determined, and the technical operation instruction of culture is established, thus the problem that the comparison benefit decreases when turtle is cultured in the pond is solved, the culture risk is reduced, the water utilization factor of the pond is simultaneously increased, and high-yield, high-efficiency, ecological and healthy mixed culture of Trionyx sinensis and full-male Pseudobagrus fulvidraco is realized.

The invention relates to a purse seine cultivation method, in particular to a purse seine high-efficiency ecological cultivation method, which adopts a three-layer three-dimensional mixed cultivation method, wherein the upper layer water body cultivates silver carps; the middle layer water body cultivates Taihu whitefish or squaliobarbus curriculus first, and then cultivates catfish; and the lower layer water body cultivates hemibarbus maculatus bleeker, and leiocassis longirostris or pseudobagrus fulvidraco first, and then cultivates turtle. The method selects proper fish species and breeding density according to the eutrophication degree of the water body and the food habits and the life habits of fishes, does not put feedstuffs, baits or fertilizers into the water body in the whole cultivation process, makes full use of the water body natural resources, changes the waste into valuable and harmful into beneficial, and reduces the water body pollution, thus the cultivation cost of aquatic products is low, the quality of the aquatic products is high, and the economic benefit is good. The method solves the technical problems that most of the prior purse seine cultivation species mainly comprise the fishes which are cultivated with feedstuffs, cause water quality eutrophication to pollute the water body, and cause ecological balance disturbance.

The invention relates to fish feed and in particular relates to fish feed for preventing enteritis of Pseudobagrus fulvidraco and a preparation method of the fish feed. The fish feed for preventing enteritis of Pseudobagrus fulvidraco is prepared from the following components in parts by weight: 22-30 parts of fish meal, 7-15 parts of bran, 8-14 parts of rice bran, 1-4 parts of compound vitamin, 4-9 parts of corn meal, 6-10 parts of Chinese gall, 3-8 parts of pomegranate bark, 3-7 parts of raspberry, 3-7 parts of Chinese yam, 2-5 parts of fructus psoraleae, 2-6 parts of rhizoma galangae, 1-5 parts of dried tangerine or orange peel, 1-4 parts of malt, 2-6 parts of fish oil, 1-4 parts of honey, 1-4 parts of olive oil. The fish feed can improve the anti bacterial resistance, the fed Pseudobagrus fulvidraco has high survival rate, low feed coefficient, high specific growth rate and strong disease resisting capacity.

The invention discloses a method for artificially propagating pseudobagrus fulvidraco fries in autumn. The method comprises the following steps: (1) selecting and cultivating parents; (2) cultivating the parents; (3) laying eggs and fertilizing, namely a, carrying out artificial spawning by adopting exogenous hormones; and b, carrying out artificial insemination; and (4) carrying out incubation management. The method for artificially propagating the pseudobagrus fulvidraco fries is specific to summer and autumn high-temperature seasons, malformation rate of the fries is reduced, and effective rate and survival rate of the fries are improved.

The invention relates to a method for cultivating Pseudobagrus fulvidraco in a rice field, particularly a stichopus japonicus fertilizer which comprises the following raw materials in parts by weight: 2-4 parts of Bacillus subtilis powder, 1-4 parts of ferment bacteria, 0.5-0.9 part of solid photosynthetic bacteria, 0.8-1.2 parts of bdellovibrio bacteriovorus, 0.2-0.6 part of vitamin additive, 10-16 parts of mineral additive, 0.2-0.4 part of chelator, 60-80 parts of hyacinth, 50-80 parts of edible fungus residue, 100-110 parts of vinasse, 40-60 parts of fishbone dust, 70-80 parts of rice bran, 10-16 parts of brown sugar, 45-55 parts of kongpo monkshood root, 40-50 parts of semen momordicae, 1-15 parts of Stellera chamaejasme and the like. A comparison shows that the composite microbial fertilizer can promote quick growth of stichopus japonicus, increase the EPA content, reduce the fish diseases and the amount of fish dying of illness, and increase the beneficial microbes in the water body, thereby enhancing the final quality and grade.

The invention relates to a Pseudobagrus fulvidraco feed. The invention is characterized in that the Pseudobagrus fulvidraco feed comprises the following raw materials in parts by weight: 0.5-0.8 part of silicate bacterium, 1-3 parts of Streptococcus faecalis, 0.6-0.8 part of Trichoderma, 2-5 parts of Rhodopseudomonas palustris, 0.3-0.5 part of microzyme, 50-60 parts of fishbone dust, 65-70 parts of vinegar residue powder, 50-55 parts of waste liquor precipitate, 40-60 parts of rice bran, 12-14 parts of brown sugar, 10-14 parts of table salt, 80-85 parts of pig manure, 45-55 parts of pork abattoir waste, 60-65 parts of aquatic, 12-16 parts of calcium bicarbonate, 14-17 parts of urea, 1-3 parts of ferric citrate, 0.6-0.8 part of vitamin B1, 0.2-0.6 part of vitamin B12, 20-30 parts of pepper and the like. A comparison shows that the composite microbial fertilizer can promote quick growth of stichopus japonicus, increase the EPA content, reduce the fish diseases and the amount of fish dying of illness, and increase the beneficial microbes in the water body, thereby enhancing the final quality and grade.

The present invention belongs to the technical field of aquaculture, and particularly relates to a method for breeding monopterus albus and pelteobagrus fulvidraco in the same pond. The method comprises the steps of: (1) arranging a net cage on the upper part of water in the pond, and breeding monopterus albus in the net cage; breeding pelteobagrus fulvidraco in the lower part of water; (2) setting the net cage to be 3 m long, 2 m wide and 1 m high, and enabling the upper end of the net cage to be above the water surface by 40 cm, and enabling the lower end of the net cage to be below the water surface by 60 cm; (3) putting 1.5 kg of monopterus albusper fries per square meter of the net cage, wherein the weight of each fry is 35 g; and putting 5000 pelteobagrus fulvidraco fries per mu, wherein the weight of each fry is 35 g; (4) respectively feeding monopterus albus expanded pellet diet with protein content of 40% and pelteobagrus fulvidraco expanded pellet diet with protein content of 35%, wherein a feeding ratio per day is 3-5% of the weight of each fry; and (5) during the rearing period, spraying lime water monthly to improve the water quality of aquaculture. According to the method, space and time of the water in the pond are fully utilized, thereby effectively improving the comprehensive benefits of the water in the pond.

The invention relates to the technical field of aquatic animal feed and particularly relates to a feed for culturing pelteobagrus fulvidraco. The feed is prepared from the following raw materials in parts by weight: 30-50 parts of fish meal, 60-80 parts of vital gluten, 50-55 parts of corn flour, 5-10 parts of shrimp shell meal, 10-15 parts of blood meal, 0.02-0.025 part of yeast powder, 3-5 parts of barbed skullcap herb, 10-20 parts of stringy stonecrop herb, 2-5 parts of Japanese milkwort herb, 1.1-1.14 parts of tangerine peels, 12-15 parts of whey powder, 0.03-0.04 part of phytase, 0.2-0.25 part of mountain ebony barks, 0.1-0.3 part of Chinese tallow leaves and 1-1.1 parts of compound vitamin. By virtue of the feed, the intake of pelteobagrus fulvidraco can be improved; the non-specific immunity and anti-oxidization capability of pelteobagrus fulvidraco are enhanced; the content of total carotenoids and the content of xanthophyll in back and belly skins of fish bodies are improved, and the body color of pelteobagrus fulvidraco can be deepened.

The invention discloses a [beta]-actin promoter, a growth hormone gene and a 3<,>-untranslated region (3<,> UTR) of pseudobagrus fulvidraco richardson, and a pseudobagrus-fulvidraco-richardson-origin transgenic vector constructed with the [beta]-actin promoter, the growth hormone gene, and the 3<,> UTR as elements. According to the invention, sequences of the pseudobagrus fulvidraco richardson [beta]-actin promoter, sequences of exon 1, sequences of partial exon 2, amino acid data-encoding sequences of the growth hormone gene, and sequences of the 3<,> UTR are acquired. The pseudobagrus-fulvidraco-richardson-origin transgenic vector is constructed with the obtained [beta]-actin promoter, the obtained growth hormone gene, and the obtained 3<,> UTR as elements. According to the invention, the transgenic vector is constructed through driving the growth hormone gene expression of pseudobagrus fulvidraco richardson by the [beta]-actin promoter of its own. With the technical scheme of the present invention, biosafety problems of faced by transgenic fish can be effectively avoided. Therefore, the invention has great practical significance and application prospects in the genetic breeding and artificial fertilizing of pseudobagrus fulvidraco richardson.

The invention relates to a method for artificial propagation of hybrid pseudobagrus fulvidraco. The method comprises parent selection and artificial spawning, wherein when the water temperature of a pool stably reaches 24 DEG C or above, artificial spawning is carried out on female pseudobagrus fulvidraco and male pelteobagrus vachelli in the pool; female pelteobagrus fulvidraco is injected twiceat an interval of 12-14 hours; male pelteobagrus vachelli is injected once, the injection of male pelteobagrus vachelli is synchronous with the second time of injection of female pseudobagrus fulvidraco, and the injection amount of male pelteobagrus vachelli is 40-60% of the total oxytocin dosage of female pseudobagrus fulvidraco; after oxytocin is injected, female pelteobagrus fulvidraco and malepelteobagrus vachelli are temporarily cultured in the pool, and the pool is filled with oxygen by an oxygen pump, wherein the oxytocin is a mixture of lutropin releasing hormone, dioxone and chorionic gonadotropin. According to the method, a spawning promoting hormone composition for fishes is used for promoting spawning so that the breeding effect of hybrid pseudobagrus fulvidraco can be obviously improved, the effect time is shortened from 30 hours to 23 hours, and the spawning promoting rate, the fertilization rate and the hatching rate reach 90.0%, 84.7% and 88.9% respectively.

The invention discloses a starvation feeding method at intervals of 7 days for pond culture of Pseudobagrus fulvidraco. The feeding method for pond culture of the Pseudobagrus fulvidraco comprises thefollowing step: after continuous feeding for n days, feeding is stopped for 1 day, and starvation (feed deprivation) is performed circularly in accordance with the period, wherein n is equal to 7-10.According to the starvation feeding method at intervals of 7 days for pond culture of Pseudobagrus fulvidraco, traditional daily feeding is replaced with intermittent (starvation) feeding, and the input quantity of external fodder of aquaculture water, excretion of culture objects and the fodder cost are overall reduced while higher level compensation growth of the culture objects is achieved; besides, by (ingestion) utilization of plankton by the culture objects in the starvation period, the eutrophication degree of the water is reduced, and the self-cleaning level of the aquaculture water and the comprehensive culture benefit are increased. The method has positive significance in promoting healthy sustainable development of the aquaculture industry.

The invention discloses a traditional Chinese medicine composition applied to the purifying of pseudobagrus fulvidraco ponds. The traditional Chinese medicine composition is prepared from the raw materials in parts by weight: 30 to 50 parts of radix rehmanniae, 25 to 38 parts of fructus forsythiae, 15 to 35 parts of semen sterculiae lychnophorae, 12 to 18 parts of pepper, 5 to 16 parts of radix paeoniae rubra, 4 to 12 parts of cyrtomium rhizome, 4 to 11 parts of aloe, 5 to 13 parts of peony root, 2 to 8 parts of fructus crataegi, 12 to 18 parts of fructus schisandrae, 1 to 6 parts of tobacco leaf, 1 to 6 parts of fructus capsici, 1 to 4 parts of fructus lycii, 0.15 to 0.35 part of botanical active extract, 1 to 3 parts of hypersorber, 0.5 to 1.2 parts of microcrystalline wax and 4 to 12 parts of binder. The traditional Chinese medicine composition disclosed by the invention can effectively purify the pseudobagrus fulvidraco ponds and remarkably improve anti-stress capacity and immunityof pseudobagrus fulvidraco; the growth speed of the pseudobagrus fulvidraco is quick, and the case fatality rate of the pseudobagrus fulvidraco is low.

The invention relates to a breeding method of pelteobagrus fulvidraco. The breeding method comprises the following steps: when fries of the pelteobagrus fulvidraco grow to 3-4 cm, putting the fries into a pond, before putting the fries into the pond, treating the fries with urea water of which the mass percentage is 0.2% for 2 minutes, and then treating the fries with a baking soda solution of 1% for 2 minutes; and 2-5 months after the fries are put into the pond, using a feed A for raising the fries, 5 months after the fries are put into the pond, using a feed B for raising the fries, and a month before the pelteobagrus fulvidraco is taken out of the pond, using a feed C for raising the pelteobagrus fulvidraco. According to the breeding method of the pelteobagrus fulvidraco, provided by the invention, the fries of the pelteobagrus fulvidraco are treated before being put into the pond, so that the environmental adaptability of the pelteobagrus fulvidraco is promoted, and the situation that the fries are dead after being put into the pond is avoided; different feeds are used for the pelteobagrus fulvidraco during different growth periods of the pelteobagrus fulvidraco, so that the growth rate and the nutrient value of the pelteobagrus fulvidraco are improved.

The invention discloses an artificial-propagation and fry-raising method for pseudobagrus fulvidraco. The method comprises the following steps: selecting almost-mature pseudobagrus fulvidraco, and independently feeding female pseudobagrus fulvidraco and male pseudobagrus fulvidraco; adding oxytocin into a feed, feeding the pseudobagrus fulvidraco with the feed for a period of time, additionally carrying out white light illumination, and adding an ovulation induction agent in a later period; carrying out mixed feeding on the female pseudobagrus fulvidraco and the male pseudobagrus fulvidraco, separating a pool into two parts by virtue of a fishing net, separating all the pseudobagrus fulvidraco to one side close to a water inlet of the pool, carrying out feeding, adding a spawning agent into water, and keeping flowing water at a flowing state; maintaining the temperature of water, regulating a pH value in the water, pumping oxygen into the pool, and waiting until spawns are fertilized and incubated; after incubation, properly enlarging a feeding range of the fries, dissolving a fry-raising into water for feeding, carrying out proper white light illumination, screening the fries withdifferent volumes after 10-20 days, separating the fries, and carrying out feeding. The invention provides the method for rapidly carrying out artificial propagation operation and fry raising. According to the method, artificial spawning induction of the pseudobagrus fulvidraco can be realized in a relatively natural state, the operation is easy and convenient, and a propagation effect is good.

The invention belongs to the technical field of artificial reproduction of fish fry, and discloses a method for removing female pseudobagrus fulvidraco and male pseudobagrus fulvidraco hybrid offspring deformed fry. The method comprises the following steps that 1, fry are temporarily raised in a cement pool; 2, micro flowing water is kept in the temporary raising pool, an uninterrupted inflation state is kept, and temporary raising is carried out for 70-75 hours; 3, the fry which are scattered, not clustered and float after gradually dying are directly removed, the fry in a clustered state are taken out through tulle or siphon or the fry are directly centralized in a fry box from a drainage opening to be taken out, and a fry culture stage is executed. According to the method, the female pseudobagrus fulvidraco and male pseudobagrus fulvidraco hybrid offspring deformed fry are removed, operation is easy, the requirement on equipment is low, and the removal rate is high.

The invention discloses a feed for improving the taste of mandarin fish. The feed is prepared from the following components: pseudobagrus fulvidraco powder, corn powder, rape seed cakes, soybean meal, internal organs of catfish, soybean straw powder, decavitamin, eucommia ulmoides, chlorite schist powder, and mixed extract of radix puerariae and mint. Various raw material components of the feed for improving the taste of the mandarin fish do not have any toxic or side effect; through reasonable matching, the feed has rich nutrients and high palatability; the feed coefficient is low; the mandarin fish is plump and tender in fish quality, and tastes fresh and delicious; moreover, the content accumulation of selenium and calcium in a mandarin fish body can be promoted.

The invention provides a preparation method of nest soil capable of improving pseudobagrus fulvidraco egg hatchability. The nest soil is prepared from, by weight, 25 parts of modified starch, 46 parts of kaolin, 17 parts of porcellanite, 16 parts of talcum powder, 16 parts of ochre, 4 parts of gypsum rubrum, 12 parts of dried tangerine or orange peel, 3 parts of beautiful millettia roots, 7 parts of fructus forsythiae, 8 parts of pericarpium granati, 9 parts of the fruits of Chinese wolfberry, 1.5 parts of dandelion, 4 parts of polypodiaceae Pyrrosia lingua, 4 parts of asclepias curassavica, 3 parts of black sesame, 0.8 part of radix campanumoeae, 4 parts of water chestnuts, 0.3 part of eel bone, 14 parts of broom cypress, 5 parts of white quartz, 3 parts of geranium wilfordii, 4 parts of glutinous rice roots, 8 parts of isatis roots, 3 parts of cocklebur fruits, 3 parts of Chinese brake herb and 7 parts of banana peel. The nest soil is used for caving and breeding of pseudobagrus fulvidraco in the reproduction stage, nest soil ingredients are reasonable in matching, the materials are easy to obtain, the preparation method is simple, and the nest soil can improve the quality of water in a nest, promote pseudobagrus fulvidraco egg metabolism, improve the pseudobagrus fulvidraco egg hatchability, improve the resistance of pseudobagrus fulvidraco fry, and improve the anti-stress ability.

The invention relates to a special breeding feed for pseudobagrus fulvidraco, wherein the breeding feed comprises the following components in parts by mass: 25-35 parts of soybean powder, 30-40 partsof DDGS, 20-30 parts of soybean lecithin, 10-15 parts of bread yeast powder, 0.5-0.6 part of trace elements, 3-5 parts of castor cakes, 5-8 parts of beer yeast, 4-8 parts of verbena, 0.5-1.5 parts ofcoptis chinensis powder, 0.6-0.8 part of Chinese prickly ash shells, 1-2 parts of raw white paeony root, 8-12 parts of shrimp and crab shells, and 4-5 parts of towel gourd powder. The feed is suitablefor breeding pseudobagrus fulvidraco, and has a certain disease resistance to water mould, ichthyophthirius multifiliis and other fish diseases.

The invention discloses a [beta]-actin promoter, a growth hormone gene and a 3<,>-untranslated region (3<,> UTR) of pseudobagrus fulvidraco richardson, and a pseudobagrus-fulvidraco-richardson-origin transgenic vector constructed with the [beta]-actin promoter, the growth hormone gene, and the 3<,> UTR as elements. According to the invention, sequences of the pseudobagrus fulvidraco richardson [beta]-actin promoter, sequences of exon 1, sequences of partial exon 2, amino acid data-encoding sequences of the growth hormone gene, and sequences of the 3<,> UTR are acquired. The pseudobagrus-fulvidraco-richardson-origin transgenic vector is constructed with the obtained [beta]-actin promoter, the obtained growth hormone gene, and the obtained 3<,> UTR as elements. According to the invention, the transgenic vector is constructed through driving the growth hormone gene expression of pseudobagrus fulvidraco richardson by the [beta]-actin promoter of its own. With the technical scheme of the present invention, biosafety problems of faced by transgenic fish can be effectively avoided. Therefore, the invention has great practical significance and application prospects in the genetic breeding and artificial fertilizing of pseudobagrus fulvidraco richardson.