25 results about "Rice field eels" patented technology

The application discloses a monoclonal antibody of a rice field eel Adam8a and a preparation method and application thereof, relates to the technical field of biological antibodies, and fills the research blank of the rice field eel Adam8a. The rice field eel Adam8a monoclonal antibody is high in specificity and titer, simple in preparation process, and controllable in cost, can be widely used for recognizing Adam8a homologous proteins in different fish ovary, and is suitable for being prepared into a fish ovary Adam8a protein detection kit, and is compared with the expression of the protein in different fish, so that the rice field eel Adam8a monoclonal antibody becomes a very convenient detection tool in the biological research of fish ovary development.

The application discloses a new strain of ricefield eel with strong disease resistance, and relates to the field of ricefield eel breeding. The method comprises the following steps: step one, selection of breeding population; step two, screening of breeding parents; step three, establishment of a basic breeding population; step four, screening of F0 generation of the basic breeding population; step five, establishment and screening of F1 generation; step six, counter-selection of F0 generation; step seven, establishment and screening of F2 generation; step eight, counter-selection of F1 generation; step nine, establishment and screening of F3 generation; and step ten, population verification. The method can not only enhance the immunity and disease resistance of ricefield eel fry and improve the survival rate, but also formulates a technical regulation for ricefield eel fry cultivation, thereby guaranteeing the healthy development of the ricefield eel breeding industry.

The invention discloses a nitrogen fertilizer application method for coordinating high-yield rice planting and healthy procypris merus breeding in a rice-fish symbiotic system, and the method is characterized in that the nitrogen fertilizer required by the whole growth cycle of rice is divided into a base fertilizer and a panicle fertilizer which are applied twice in two specific periods, and the mass ratio of the application amount of the base fertilizer to the application amount of the panicle fertilizer is 7: 3. Through reasonable fertilization control, the nitrogen fertilizer absorption and utilization rate is effectively improved, and the yield of rice in the rice-fish symbiotic system is stabilized. In addition, the nitrogen fertilizer application method can reduce the toxic effect of ammonia nitrogen in the rice field water body on procypris merus, the high and stable yield of rice is guaranteed, meanwhile, the stocking density of the procypris merus in the rice field can be increased, and the yield and economic benefits of the procypris merus are increased.

The application discloses an Indel molecular marker for identifying a rice field eel strain in a southwest region, wherein the nucleotide sequences of the Indel molecular marker are respectively represented by SEQ ID NO: 9-12 or represented by SEQ ID NO: 13-16. The application also provides primer pairs for amplifying the Indel molecular marker. The genomic DNA of the rice field eel strains in different regions is amplified by using the primer pairs of the Indel molecular marker, and the rice field eel strains in the southwest region can be quickly screened according to the amplified fragments. The application of the Indel marker to practice is beneficial to the rapid genetic identification of the rice field eel strains in the southwest region, accelerates the domestication breeding of wild germplasm resources, and realizes precision breeding.

The invention discloses a fluorescent quantitative PCR (polymerase chain reaction) method for ricefield eel rhabdovirus, and belongs to the technical field of virus molecule detection. The purpose of the invention is not subversively replaced by the prior art, but is optimization based on the defects of complementing the defects and improving the performance of the existing detection requirements: the specificity advantage of molecular detection is reserved, and the defects of sensitivity and quantitative capability are made up through a fluorescent quantitative technology; according to the method, the operation process is simplified to adapt to basic application, the popularization threshold is lowered through compatibility design, a set of accurate, rapid, easy-to-use and economical CrERV detection scheme is finally formed, technical guarantee is provided for disease prevention and control of the ricefield eel culture industry, and meanwhile a reference thought is provided for fluorescent quantitative detection technology development of other aquatic viruses.

The utility model provides a kind of eel oxygenation transport case convenient for air circulation, including box, body inside upper end is fastened with second support frame, net cage is connected on second support frame, the top of net cage is fastened with mounting frame, and mounting frame is located on the top of second support frame;First support frame fastened with box is equipped above second support frame, and the sidewall upper end of box is equipped with multiple air holes, air hole is located between second support frame, first support frame, and annular support seat is fastened in the bottom of box, the height of annular support seat is greater than the distance between first support frame and the top of box;Multiple connecting pipes are fastened in the sidewall lower end of box, and the end of connecting pipe located in the box is fastened with spray head.The utility model is provided with oxygenation pump, which can effectively oxygenate the breeding water in the box, the air hole can improve the ventilation of the box, ensure the air circulation, and improve the survival rate of eel fry.

The application belongs to the field of aquatic organism breeding, and particularly relates to a rice field eel D2HGDH The application relates to a SNP site combination related to a gene and resistance to aquatic pathogenic viruses and application thereof. D2HGDH The SNP site combination related to the gene and resistance to the rice field eel rhabdovirus is located at the 149th, 365th, 394th, 902nd and 939th base from the start codon of the gene. D2HGDH The detection of the SNP site combination of the gene can screen the individuals resistant to the rhabdovirus of the rice field eel. Therefore, the SNP molecular marker can be used for molecular marker assisted breeding of the rice field eel, accelerates the breeding of the disease-resistant rice field eel, and provides a reference for the disease-resistant breeding of the rice field eel.

The invention relates to the technical field of breeding, in particular to a feeding device and method for factory ricefield eel breeding, and the feeding device comprises a charging barrel, a feeding port, a boss, a connecting block, a side shaft and a spiral cutter. According to the feeding equipment and method for factory-like ricefield eel breeding, through alternate arrangement and sliding fit of bosses and connecting blocks, an adjustable multi-layer stirring structure is formed, through cooperation with a spiral cutter on a side shaft, materials in a charging barrel can be stirred and crushed in all directions, through meshing fit of a first outer gear ring and a gear ring, the feeding efficiency is improved, and the feeding efficiency is improved. The connecting ring drives the top shaft and the connecting piece to rotate around the axis of the top shaft while driving the connecting piece to slide in the circumferential direction, and due to the compound motion, the spiral cutter on the side shaft can do circular motion along with the connecting piece to cover a larger stirring range in the charging barrel, and the material stirring uniformity and the crushing efficiency can be greatly improved.

The application discloses an Indel molecular marker for identifying Ophiocephalus argus strains in Guangdong and Guangxi regions, wherein the nucleotide sequence of the Indel molecular marker A is shown in SEQ ID NO:5 or SEQ ID NO:7; and the nucleotide sequence of the Indel marker B is shown in SEQ ID NO:6 or SEQ ID NO:8. The application also provides primer pairs for amplifying the Indel molecular marker. The genomic DNA of Ophiocephalus argus strains in different regions is amplified by using the Indel molecular marker primer pairs, and the Ophiocephalus argus strains in Guangdong and Guangxi regions can be quickly screened according to the amplified fragments. The Indel marker provided by the application can be applied to the rapid identification of specific geographical strain germplasm resources, so that excellent properties of the strain can be obtained, and finally, precision breeding can be realized.

The invention discloses a ricefield eel fertilized egg artificial incubation system and an automatic siphoning method thereof. A high-level water tank automatically supplements water through a floating ball valve, and intermittent water supply is achieved under the triggering of a water level through an inverted-U-shaped siphon; the annular loop pipeline uniformly distributes water flow to the hatching barrels connected in parallel; an inverted-cone-shaped flow guide cover is arranged in the barrel to buffer water flow and is matched with a 20-mesh flat-bottom net and an anti-escape net cover to form an egg particle suspension area. The artificial hatching system for the fertilized eggs of the finless eels runs completely depending on gravity, simulates the natural hatching environment of the finless eels, has the advantages of zero energy consumption, low maintenance and high survival rate, and effectively solves the problem of large-scale production of finless eel fries, and the hatching capacity of a single barrel reaches 100,000.

The application discloses a high-temperature and high-density-based rice field eel training method and device, and the training method is used for training the rice field eel seedlings in a high-temperature and high-density environment, so that the seedlings are trained in the high-temperature and high-density environment, the adaptability of the seedlings to high-density breeding in the later period is improved, and the survival rate of the seedlings in summer is improved; the rice field eels with weak constitution, transport damage and high stress are selected out through a temperature transition period, and then are sold; then, the temperature of water is slowly increased for high-temperature training, the high-temperature training mortality is reduced, and the economic loss is reduced; therefore, the method has the characteristics of low cost and good benefit.

The utility model relates to a water surface walking platform for cage culture of ricefield eels, which comprises a walking supporting piece and a plurality of lighting assemblies, the plurality of lighting assemblies are fixed on the walking supporting piece at intervals, each lighting assembly comprises a light-emitting piece and a power supply piece, and the light-emitting pieces are connected on the walking supporting piece; the power supply part is connected to the walking supporting part and can supply power to the light-emitting part. The technical scheme has the beneficial technical effects that the power supply part and the light emitting part are arranged on the walking supporting part, so that a feeder can clearly see the specific position of the walking supporting part at any time when walking on the walking supporting part at night, and the risk that the feeder walks on the walking supporting part at night and falls into water is avoided. The problem that in the prior art, when a feeder walks at night, due to the illumination problem, the risk of falling possibly exists is solved.

The invention discloses a soft particle starter feed for breeding monopterus albus fries and a preparation method of the soft particle starter feed, and belongs to the technical field of aquaculture. The feed consists of fish meal, defatted euphausia superba powder, mixed protein powder, yolk powder, a multi-vitamin and multi-mineral premix, sodium alginate, alpha-starch and betaine. The preparation method comprises the following steps: carrying out superfine grinding, mixing and stirring, carrying out twin-screw extrusion curing sterilization, granulating soft granules, and carrying out rapid freezing and shaping. The feed is comprehensive in nutrition, good in palatability and water stability and high in digestibility, can significantly improve the ingestion enthusiasm, survival rate and weight gain rate of the ricefield eel fries, solves the problems of high hardness, poor food calling performance, insufficient stability in water and the like of traditional feed, and is suitable for large-scale artificial breeding of ricefield eels.

The application relates to a metabolic reinforced ecological circulating water aquaculture system suitable for relay breeding of ricefield eels, wherein a breeding pond unit is a variable-width runway type breeding pond, the width ratio of the water inlet end to the water outlet end is 2:1-3:1, a plurality of eel nests are hung in the breeding pond, bait tables are arranged on each eel nest, and the bait tables are used for feeding ricefield eels; a water quality purification unit sequentially comprises a drum type solid-liquid separator, a moving bed type biological filter and a drip type biological filter in the water circulation direction, a pipeline at the water outlet end of the drip type biological filter is provided with an ultraviolet disinfection lamp; a temperature control unit comprises an air source heat pump and a refrigerating machine, and is communicated with the pipeline of the moving bed type biological filter to regulate and control the water temperature; an oxygenation unit is an air aeration oxygenation machine, and is communicated with the breeding pond unit and the moving bed biological filter; and a water circulation regulation unit is a circulating pump with a frequency converter, and is matched with a closed loop water circulation pipeline to adjust the hourly circulation times of the system water body.

The invention relates to the technical field of aquaculture, and discloses equipment and a system for indoor clean culture of ricefield eels, which comprise a temporary culture pond, a first water storage cylinder, a second water storage cylinder, a delivery pump, a centrifugal pump and an oxygenation system. Nozzles are arranged in the temporary rearing pond clockwise at an angle of 45 degrees, rotating water flow is generated to induce the ricefield eels to move reversely, and the water flow speed can be controlled within the range of 0.3-0.8 body length / second; the first water storage cylinder and the second water storage cylinder are respectively used for activating water quality and enhancing nutrition, and the water temperature is maintained at 28-30 DEG C through the heating element and the temperature sensor. The control system realizes automatic regulation and control of water temperature, water flow, dissolved oxygen and water level. The breeding environment is optimized through multi-module collaborative design, and the growth quality and breeding efficiency of ricefield eels are remarkably improved.

The utility model relates to an inhabiting device for fish culture. The inhabiting device comprises a base, a supporting rod and a plurality of bionic threads. The base is fixed to the water bottom, and the bottom end of the supporting rod is connected with the base; the bionic wires are all arranged at the upper part of the base, and the bionic wires are uniformly arranged around the supporting rod, so that the bionic wires form a canopy-shaped structure; the bionic silk is used for replacing a plant root system to provide a shading habitat environment for juvenile fishes of sciophilous fishes such as ricefield eels, compared with plants, the bionic silk can be recycled, the bionic silk cannot rot, water cannot be polluted, and the survival rate of the juvenile fishes is increased; besides, supporting materials are arranged to support the bionic silk, inhabiting space suitable for adult fishes is provided in combination with space design, and the device can also be applied to the cultivation process of shade-loving adult fishes.

The present application relates to aquatic product slaughtering equipment technical field, especially to a kind of ricefield eel slaughtering equipment.It includes: shell, for structure protection;Support frame, bolt connection in the inside of shell;Profiling accelerated feeding mechanism, for the eel to be slaughtered is transported;Dissecting and gutting linkage mechanism, for the eel transported by profiling accelerated feeding mechanism is opened and cleaned;Segmentation mechanism, for the eel after cleaning is segmented, and the eel after segmentation is sent out through discharge pipeline.The present application provides a kind of ricefield eel slaughtering equipment, can let eel enter the inside of profiling accelerated feeding mechanism in the inside of feeding water tank, through profiling accelerated feeding mechanism, eel is stably transported, while through the work of dissecting and gutting linkage mechanism, eel is opened and cleaned, and through the work of segmentation mechanism, eel is segmented and transported out, while after segmentation, through the water washing of bamboo joint pipe, blood dirt is washed.

This invention discloses a batch centralized hatching device for swamp eels, comprising an installation frame, with multiple support plates equidistantly connected inside the frame. Each support plate has a support frame directly below it, and several limiting sleeves are connected within the support frames. The limiting sleeves have an upward-opening trumpet shape in cross-section. A rotating shaft is rotatably connected to the top center of each support plate, with a sleeve coaxially and securely connected to the outside of the shaft. A motor is securely fastened to the top of the shaft. Symmetrically symmetrical through holes are provided on the top of each support plate, and guide wheels are rotatably connected to the side of the through holes closest to the motor. Traction ropes are symmetrically connected to the top of the support frames, with the ends of the ropes passing through the through holes and winding around to the top of the guide wheels. Driven by a motor, this invention allows for the raising and lowering of the support frames, thereby enabling continuous suspension and settling of fertilized eggs in the water. This ensures sufficient dissolved oxygen, prevents fertilized eggs from clumping and molding, promotes embryonic development, and effectively improves the hatching rate of swamp eels.

The utility model provides a kind of quantitative feeding device for rice field eel culture, including material containing box, the top coaxial connection of material containing box has sealing cover, and the top coaxial connection of sealing cover has connecting pipe, connecting pipe is coaxially connected with electric push rod in, and electric push rod free end penetrates sealing cover and extends to its bottom;Electric push rod's free end is coaxially connected with pressing plate, the diameter of pressing plate is equal to the inner diameter of material containing box, and multiple guide rods that penetrate sealing cover are vertically connected in the top of pressing plate;And multiple through holes are set in the bottom of material containing box, and conical tube is coaxially arranged in the bottom of through hole, and conical tube is fixedly connected with the bottom of material containing box.The utility model can control electric push rod to push pressing plate to move downwards, and the number of conical tube and the inner diameter of material containing box are certain, i.e., the height of pressing plate can be controlled to realize the quantitative feeding of feed, save a lot of manpower, ensure the quantitative feeding of feed, and effectively improve the practicability of device.

The invention discloses monopterus albus bone antioxidant peptide as well as a preparation method and application thereof, and belongs to the technical field of antioxidant active peptide. The amino acid sequence of the monopterus albus bone antioxidant peptide is shown as SEQ ID NO.2, and the ABTS activity and ORAC activity of the monopterus albus bone antioxidant peptide are higher than those of Trolox. The polypeptide is also provided for activating enzyme and non-enzyme in-vivo antioxidant systems of zebra fish by regulating a Keap1-Nrf2 pathway, so that the resistance of the organism to oxidative stress is enhanced. The potential application of the monopterus albus bone antioxidant peptide provided by the invention in functional products has important significance.

The application discloses application of an orange bony fish bone antioxidant peptide in preparation of antioxidant products and belongs to the technical field of antioxidant active peptides.The amino acid sequence of the orange bony fish bone antioxidant peptide is shown as SEQ ID NO.3-14, the 12 polypeptides are proposed to have antioxidant activity for the first time, the ABTS activity and ORAC activity of the polypeptides are 1.70-4.29 times and 2.79-5.90 times of Trolox respectively.The polypeptides are proposed to activate the enzyme and non-enzyme antioxidant system in zebra fish by regulating the Keap1-Nrf2 pathway, so that the resistance of the body to oxidative stress is enhanced.The potential application of the orange bony fish bone antioxidant peptide in functional products has important significance.

1. The name of the design product: handheld yellow hook. 2. The use of the design product: for fishing. 3. The design points of the design product: in shape. 4. The picture or photo that best shows the design points: the reference drawing in use. 5. The top view of component 1 has been omitted because its design points have been shown in other views.

The invention discloses an ecological planting and breeding method and planting and breeding system for vegetable eels, and the method comprises the following steps: treating breeding water after breeding eels to remove residual bait with the particle size of more than 0.15 mm and eel excrement, and then treating the breeding water by sewage treatment bacteria to serve as a nutrient solution of water spinach planted and bred in a soilless culture mode; the nutrient solution is used as a water body for culturing ricefield eels again after nutrients are absorbed by the water spinach, residual baits with the particle size smaller than or equal to 0.15 mm and ricefield eel excrement are dissolved in the culture water, and the residual baits with the particle size smaller than or equal to 0.15 mm and the ricefield eel excrement are converted into nutrients needed by water spinach planting and breeding through the sewage treatment bacteria. Therefore, the culture water, the nutrient solution and the water body for culturing the ricefield eels continuously circulate, the culture sewage is finally purified into clean culture water, external water is not required to be replaced, a high-cost filtering system is not adopted, the water spinach can be harvested while the ricefield eels are harvested, and the economic benefit is high.

The invention discloses a living body storage device and method based on hibernation habits of finless eels, and relates to the technical field of aquaculture equipment.The living body storage device is composed of a refrigeration house, a breeding device and a temperature control device, the breeding device is placed in the refrigeration house, and the temperature control device is installed in the breeding device and used for monitoring and adjusting the temperature in the breeding device; the electric power source of the temperature control device and the refrigeration house is the State Grid of China, accurate and automatic temperature regulation and control of the water temperature can be achieved, circulation of aquaculture water is achieved by automatically changing water at regular time, and therefore the storage time of the ricefield eels is prolonged.