36 results about "Pompano" patented technology

Compound Chinese medicinal herb immunopotentiator for golden pompano is prepared from the components by weight: 10 to 20 percent of radix scutellariae, 10 to 15 percent of radix astragali, 10 to 15 percent of honeysuckle, 10 to 15 percent of largehead atractylodes rhizome, 10 to 15 percent of safflower, 5 to 10 percent of radix-polygoni multiflori, 5 to 10 percent of eucommia bark leaf powder, 5 to 10 percent of liquorice and 5 to 10 percent of radix paeoniae alba. The invention has the following advantages that the compound Chinese medicinal herb immunopotentiator can be used as feed additive in golden pompano feed and is added in the golden pompano feed by only 0.1 to 0.2 percent, can obviously increase the organism immunity and improve the survival rate of the golden pompano, has no adverse effect to the palatability of feed, and has stable raw material sources, low cost and simple production technology.

The invention provides trachinotus ovatus beta-thymosin and application thereof, and relates to the field of molecular biology. The cDNA nucleotide sequence of the trachinotus ovatus beta-thymosin is shown as SEQ ID NO. 1, and the amino acid sequence is shown as SEQ ID NO. 2. The invention also provides application of the trachinotus ovatus beta-thymosin to anti-bacterial disease medicine, and a recombinant protein expression method of the trachinotus ovatus beta-thymosin. The beta-thymosin provided by the invention can obviously inhibit the growth of pathogenic bacteria; after the injection into the fish is finished, the fish disease-resistant capability can be obviously improved.

The invention discloses a preparation method of probiotics-containing fish feed, which comprises the following steps: A. preparing primary ingredients in percentage by weight: 47 to 57% of fish meal, 10 to 20% of soybean meal, 3 to 6% of shrimp meal, 3 to 6% of squid liver paste, 20 to 26% of flour, 1 to 3% of minerals and 0.3 to 1% of choline chloride are uniformly mixing, adding water, mixing and puffing to obtain puffed granular material; B. preparing a probiotics-containing spray: uniformly mixing 30 to 50% of fish oil, 2 to 5% of vitamin, 20 to 50% of probiotics and 15 to 40% of water in percentage by weight; and C. spraying the spray on the puffed granular material, wherein the weight percent of the primary ingredients is 90 to 95% and the weight percent of the spray is 5 to 10% Tests prove that, the preparation method adopting process of probiotics spraying subsequent to buffing and granulation can improve the attraction effect of the feed and effectively maintain the stability and suspension property of the feed in water; and the operation of probiotics spray is not carried out in an high-temperature high-pressure environment, which is beneficial to maintenance of colony activity. According to the invention, breeding cost is lowered, great contribution to guaranteeing food safety is achieved, and accordingly, wide application prospect of golden pompano is opened up.

The invention discloses a system for characterizing macrophage phagocytosis microorganisms of pompanos. The pompanos are dissected and separated through a surgical knife and tweezers to obtain heads and kidneys; the heads and the kidneys are abraded through a cell sieve to obtain cell suspension containing macrophages, and the macrophages of the heads and the kidneys are obtained through a Percoll solution density gradient centrifugal tube. The survival amount of cells cultured in vitro through a trypan blue dyeing manner is counted, and the survival condition in one week is detected. The system is used for carrying out a phagocytosis experiment, and phagocytosis conditions of nocardia, yeast and the like of the pompanos by the head and kidney macrophages of trachinotus ovatus are determined, and a phagocytosis function is observed. An experiment result of the system shows that the macrophages of the pompanos can be extracted very well, and the survival condition and the phagocytosis condition of the macrophages of the pompanos can be observed very well. The system disclosed by the invention lays a foundation for further researches on physiology, pathology and toxicology of the macrophages of the pompanos.

Hepatinica used for golden pompano is prepared from the following components by weight: 20 to 30 percent of vitamin E, 20 to 30 percent of vitamin C- polyphosphonate, 10 to 15 percent of sinkaline, 5 to 10 percent of L-Carnitine, 5 to 10 percent of lecithin, 5 to 10 percent of Rhizoma Atractylodis Macrocephalae, 5 to 10 percent of medlar, and 5 to 10 percent of Alisma orientale. The hepatinica can be used as additive of golden pompano feed and is added in the golden pompano feed only by 0.1 to 0.2 percent, can prevent fatty infiltration of the golden pompano, strengthen the liver function, improve the feed efficiency and promote the growth of the golden pompano, improves the survival rate of golden pompano, has no poor influence to the palatability of feed, and has stable raw material resources, low price and simple production technology.