44 results about "Growth stimulation" patented technology

The invention relates to apparatus and method for ultrasonically stimulating cartilage growth. The apparatus includes at least one ergonomically constructed ultrasonic transducer configured to cooperate with a placement module or strip for placement in proximity to an area where cartilage growth is desired. The apparatus also utilizes a portable, ergonomically constructed main operating unit constructed to fit within a pouch worn by the patient. In operation, at least one ultrasonic transducer positioned in proximity to an osteochondral site is excited for a predetermined period of time. To ensure that at least one ultrasonic transducer is properly positioned, and to insure compliance with a treatment protocol, a safety interlock is provided to prevent inadvertent excitation of the at least one ultrasonic transducer.

The invention provides an applicator for skin treatment having one or more RF electrodes. An article is located between the electrodes, such as a roller or flexible belt containing one or more protruding pins electrically isolated from the RF electrodes. The invention also provides a system for skin treatment comprising the applicator of the invention and a control unit. The invention further provides a method of treating skin disorders in which a section of the skin is heated while, essentially simultaneously, piercing one or more holes in the heated section of the skin. The method of the invention may be used, for example, in collagen remodeling.

The invention discloses a breeding method for medicinal silky fowl. The breeding method mainly comprises the steps of constructing a henhouse, brooding, breeding, disinfecting the henhouse and the like. The survival rate of chicken is greatly increased by adopting the breeding method provided by the invention for breeding silky fowl. According to the breeding method provided by the invention, a microbial technology is effectively combined with an ecological breeding technology, so that a powerful dominant bacterial community is formed in vitro and in vivo of animals under the effects of probiotic stock solution, fodder, drinking water and spraying sanitization, and a pathogenic bacteria colony is restrained and eliminated; a large quantity of nutrition and hormone matters, such as amino acid, protein, vitamin, various bio-chemical enzymes, antibiotics and somatomedin, are secreted and compounded, so that all the organ functions of animal bodies are adjusted and promoted; the feed conversion rate is increased; various functions, such as immunization, nutrition and growth stimulation are generated for the animals; obvious effects, such as preventing and curing diseases, increasing survival rate, promoting growth and breeding, lowering cost, eliminating fecaluria smell, purifying environment and increasing yield and income are achieved.

Bone growth for fusion promotion is stimulated in a mammalian patient in need thereof. Bone growth stimulation is achieved by implanting an electro-conductive bone growth stimulating implant in a region in the patient where bone growth is desired. An external device is worn by the patient to produce a direct current in the implant whereby bone growth is stimulated. The external device produces a magnetic field that induces an electric current in the implant. The electric current stimulates bone growth. The implant contains strips of a biocompatible conductive metal, such as, for example, nickel, gold or titanium. The strips can also be made of a conductive polymer such as for example, graphene. Implants to treat spinal cord pain are also disclosed.

A stem-cell-seeded porous scaffold implant and electromagnetic growth stimulation for treating or augmenting a breast tissue defect in a patient.

This invention provides a device that uses a lens to focus electromagnetic energy to a singular three dimensionally focused point. The invention has three-dimensional target positioning capability. This invention can be used in tissue ablation, tissue thermography, tissue heating, and tissue hyperthermia. Possible specific treatment applications include, but are not limited to: cancer ablation, scar tissue ablation, epithelial tissue ablation, infected tissue ablation, herniated disk removal, tissue growth stimulation. The main advantage of this device is that it maximizes the effect on the target tissue and greatly reduces the effect of the surrounding tissue to the point of there being close to no side effects from radiation therapy.

Fucose galactose carbohydrates have been shown to induce neuronal outgrowth. The invention includes methods of inducing neuronal outgrowth using carbohydrates, assemblies, and polymers bearing fucose-galactose moieties, as well as associated proteins. Cell growth can be stimulated in cells in culture or in cells within an animal or patient. Growth stimulation has application to understanding and treatment of neurodegenerative diseases including, for example, Parkinson's disease, Alzheimer's disease and multiple sclerosis and conditions such as stroke, brain injury and spinal cord injury. Such compounds, polymers, and assemblies also can be used to increase neural stem or progenitor cells in culture or in an animal, and to enervate engineered tissue.

The invention discloses an early rice seedling cold-proof agent and a using method thereof. The early rice seedling cold-proof agent is a solution formed by mixing, by weight, 30-40 parts of glucose, 20-30 parts of allantoin, 6-10 parts of aspirin, 0.3-0.5 part of gibberellin and 500 parts of water. The early rice seedling cold-proof agent has the advantages that the concentration of cell sap can be improved through the glucose, the cold hardiness of the cells can be improved through the allantoin, the aspirin has the antibacterial function, and the gibberellin has the growth stimulation function. After scientific compatibility of medicines, the cold resistance capacity of early rice seedlings can be improved, and the seedlings can be better promoted to grow strong.

Provided is an applicator for skin treatment having one or more RF electrodes. An article is located between the electrodes, such as a roller or flexible belt containing one or more protruding pins electrically isolated from the RF electrodes. Also provided is a system for skin treatment including the applicator and a control unit. Further provided is a method of treating skin disorders in which a section of the skin is heated while, essentially simultaneously, one or more holes are placed in the heated section of the skin. The present method may be used, for example, in collagen remodeling.

The invention discloses a method for inducing high-yield astaxanthin of Haematococcus pluvialis by a trichromatic light compound culture, which belongs to the technical field of microalgae biologicalfunction development. The method was applied to the culture of Haematococcus pluvialis with light intensity of 2000-3000lx, and the light intensity of 7000-12000 lx of blue, red and white combined light for mixed illumination. The technical scheme of the invention overcomes the technical prejudice existing in the prior art that only the red light or the red-white mixed light is used to promote thegrowth of the algae strain, and only the blue light or the blue-white mixed light is used to promote the astaxanthin accumulation of Haematococcus pluvialis. The growth and astaxanthin accumulation of Haematococcus pluvialis were stimulated by white, blue and red light simultaneously. As prove by experiments, that technical effect of simultaneous growth stimulation and product induction of Haematococcus pluvialis by use of trichromatic light is significantly superior to that of using only monochromatic light or mixing only with white light. The invention is characterized in that the trichromatic light is used for stimulating the growth of Haematococcus pluvialis and inducing the product.

The invention discloses a method for rapidly application of an esteya vermicola solid culture product in prevention and treatment of a pine wilt disease, and belongs to the technical field of biological control. According to the method, a certain proportion of nutrient substances, a protective agent, a growth stimulating factor, a spore stimulation factor and an adhesive are added into the solid culture product and are uniformly mixed, then a harmless plasticizer is added into the solid culture product, the solid culture product is pressed and processed into a long-rod-shaped product, finallya layer of wafer is wrapped around the surface of the long-rod-shaped product, the whole production process is isolated from the outside environment, manual operation is not needed, and therefore pollution caused by human factors or exposure to the external environment is reduced; during application, only two operation links of drilling and knocking-in are needed, a high-pressure plug does not need to be used, and therefore manpower and material resources are greatly reduced; meanwhile, the storage life of the product is greatly prolonged, and the biological activity of the product is remarkably improved; and the problem that a solid culture product cannot be directly applied to biological control can be solved. The method has the advantages of being simple in operation and low in cost when being used for the prevention and treatment work of woodland.

Devices, systems, and methods for therapies involving the application of an electrical signal within the body of a subject involve the use of an implanted piezoelectric nanogenerator to provide a self-generated electrical signal without the use of batteries. The electrical signal stimulates healing of a tissue, such as bone, or provides pain relief by inhibiting neuronal pain signals. An external signal generator induces mechanical stress in an implanted piezoelectric nanomaterial, which produces the electrical signal.

A hybrid cytokine comprising the B-chain of hepatocyte growth factor and IL-7, linked by a linker molecule, having pre-pro-B growth stimulating activity.

Fucose galactose carbohydrates have been shown to induce neuronal outgrowth. The invention includes methods of inducing neuronal outgrowth using carbohydrates, assemblies, and polymers bearing fucose-galactose moieties, as well as associated proteins. Cell growth can be stimulated in cells in culture or in cells within an animal or patient. Growth stimulation has application to understanding and treatment of neurodegenerative diseases including, for example, Parkinson's disease, Alzheimer's disease and multiple sclerosis and conditions such as stroke, brain injury and spinal cord injury. Such compounds, polymers, and assemblies also can be used to increase neural stem or progenitor cells in culture or in an animal, and to enervate engineered tissue.

The invention discloses an industrialized production method for high galactosylated modification recombination human erythrocyte growth stimulation protein utilizing serum-free medium used for steadily improving protein expression index of Darbepoetin Alpha. The method comprises the following steps: adopting serum-free medium to cultivate CHO cell strain in a suspending manner in a biological reactor; expressing the Darbepoetin Alpha in high efficiency in the CHO cell. The invention further discloses the serum-free medium adopted by the industrialized production method for high galactosylated modification recombination human erythrocyte growth stimulation protein. The serum-free medium component used for steadily improving protein expression index of Darbepoetin Alpha is utilized; meanwhile, the CHO cell can efficiently and steadily express Darbepoetin Alpha; expression is stable; cultivation method is brief, and is suitable for large scale cultivation.

The invention relates to a testing device for quantitatively detecting a soluble growth stimulation expression protein 2. The testing device comprises a testing card and a quantitative immunity analyzer, wherein the testing card comprises a blood filtering pad, a colloidal gold pad, a nitrocellulose membrane, a water absorption pad, a back liner and a clamping shell, antihuman growth stimulation expression protein 2 antibodies marked by colloidal gold are fixed on the colloidal gold pad, detection lines coated by the antihuman growth stimulation expression protein 2 antibodies and quality control lines coated by IgG (immunoglobulin G) antibodies are arranged on the nitrocellulose membrane, a sample loading region and a detection region are arranged on the clamping shell, and the position of the detection region corresponds to the positions of the detection lines and the quality control lines; the quantitative immunity analyzer is used for detecting the optical density of the detection region of the testing card. The testing device is simple and convenient to operate and is used beside a bed, and the detection range is widened to reach 200 ng / ml maximally, so that the estimation of the prognosis and orders of severity of heart failures of different degrees is strengthened.

The invention relates to a method for automatically constructing a stable electrocatalytic bacterial biofilm. The method comprises the following steps: firstly assembling a microbe electrochemical reactor, and connecting circuits, wherein the first circuit is as follows: a fixed resistor is connected between an anode and a cathode in series, and the second circuit is as follows: an adjustable direct-current power supply, an ampere meter and a work contact of a circulating time relay are connected between the cathode and the anode; then injecting a bacteria growth medium solution into an anode chamber, inoculating a bacteria source, prestarting a microbe anode by switching on the first circuit, then controlling pulse current between the anode and the cathode to be gradually increased at preset velocity according to the potential change of the anode by switching on the adjustable direct-current power supply of the second circuit, wherein the pulse current is immediately lowered by 40%-60% when the potential of the anode is increased to a limit value and enters a new round of growth stimulation, and the electrocatalytic bacterial biofilm is completely constructed till the pulse current value reaches a preset current limit value and is stable at the preset current limit value for more than 10 hours. The electrocatalytic bacterial biofilm disclosed by the invention can be stably operated with the relatively high working current.

The invention relates to a preparation method of a poultry embryo egg extracting solution, and belongs to the technical field of health-care foods. The preparation method comprises the following technology steps of selecting poultry fertilized eggs, preforming conventional surface cleaning treatment, and performing conventional incubation for 8-12 days; after poultry embryos are determined to grow normally, performing cleaning, and disinfecting the surfaces of eggshells; collecting all substances in the embryos and eggs, and performing crushing with a crusher for 1-5 minutes so as to obtain homogenate; adding cane sugar which is 20-50% of the homogenate to the crushed homogenate, enabling the cane sugar to dissolve, and performing transferring to an ice bath; performing crushing treatment on the homogenate, wherein the crushing treatment comprises ultrasonic treatment, French high-pressure extruding treatment and / or grinding treatment with glass beads; performing centrifugation treatment on the homogenate; subpacking supernatant obtained through centrifugation in a sealed container, and performing pasteurization; or performing separation treatment on the supernatant obtained through centrifugation by a chromatographic fractionation method to obtain different particular protein and performing subpacking; and performing preservation at 4 DEG C or minus 20 DEG C. According to the preparation method disclosed by the invention, high-activity growth stimulation factors, high-activity nerve growth factors, high-activity active polysaccharide and high-activity active polypeptide are extracted and reserved to the maximum extent.

The invention discloses an immunodetection reagent for soluble ST2 (growth stimulation expressed gene 2) protein as well as a preparation method and a detection method of the immunodetection reagent.The immunodetection reagent comprises enzyme-labeled soluble ST2 protein and an indication reagent which is used for detecting a soluble ST2 protein antibody and enzyme-labeled soluble ST2 protein compound, wherein the enzyme-labeled soluble ST2 protein is prepared from the soluble ST2 protein and glucose dehydrogenase by coupling. With adoption of the immunodetection reagent for the soluble ST2 protein, content of the soluble ST2 protein in samples of human blood and the like can be determined precisely and rapidly. Compared with the detection reagents in the market, the detection reagent hasthe advantages of being convenient, fast, high in sensitivity and specificity, accurate in quantification and the like, and clinical popularization and application are facilitated.

The invention discloses a Bacillus spp culture medium with coconut water as a main raw material. The culture medium comprises: (1) a growth stimulation culture medium ( / L); (2) a liquid amplification culture medium ( / L); and (3) a colony counting culture medium ( / L). According to the invention, economical, cheap and widely available coconut water is used as the main raw material for the Bacillus spp culture medium, and a scientific and reasonable application method of the Bacillus spp culture medium is constructed at the same time; with the application method, the culture medium can meet requirements of scale-up culture of Bacillus spp; a leftover of coconut processing in Hainan province, i.e., coconut water, is made full use of, the Bacillus spp culture medium with coconut water as the main raw material is created for the first time, a good cheap culture medium formula is provided for development of scale-up culture of Bacillus spp in the future, and the Bacillus spp culture medium has a good application prospect; an effective approach is provided for high-valued utilization of coconut water, an added value of coconut processing can be obviously increased, and pollution of the industry of coconut processing to the environment can be reduced at the same time.

The invention discloses a preparation method and application of pseudomonas chlororaphis for resisting disease and promoting growth. A strain is pseudomonas chlororaphis HT66CCTCC No:M2013467. A main metabolite of the strain comprises phenazine-1-phenazine and phenazine-1 formamide. The strain is inoculated into a microorganism culture medium and is fermented to obtain a thallus with high concentration and the metabolite, and can be mixed with materials like powder active carbon, bentonite, swell soil, kaolin, diatomite, zeolite, calcium carbonate and the like to prepare a microbial agent; the quantity of viable organisms in a finished product is 1-8.5*10<9>cfu / mL. The tablet laboratory verifies that the pseudomonas chlororaphis has good control effect of pathogenic bacteria of rice sheath blight diseases, cotton fusarium wilt, gaeumannomyces graminis and watermelon fusarium wilt, and also has a good promotion function in the growth of a part of plants.

The invention discloses a time-resolved fluoroimmunoassy test strip used for quantitative determination of sST2, and a preparation method thereof. The time-resolved fluoroimmunoassy test strip used for quantitative determination of sST2 comprises a base plate; a sample pad, a conjugate pad, a coating membrane, and an absorbent paper are arranged on the base plate in a staggered manner; the coating membrane is arranged on the middle part of the base plate; the upper end of one side edge of the coating membrane is covered by the conjugate pad, and the upper end of the other side edge is covered by the absorbent paper; and the edge of one side, far from the coating membrane, of the conjugated pad is covered by the sample pad. Compared with the prior art, the time-resolved fluoroimmunoassy test strip possesses following advantages: time-resolved fluoroimmunoassy is introduced into detection of soluble growth stimulation expressed gene 2 (sST2), quantitative determination of single portion sST2 is realized via combination with a time resolved fluorescence detector, sensitivity is high, intra-assay and inter-assay difference is low, great convenience is provided for clinical application, operation of the preparation method is simple, the preparation method is suitable for large scale production, and the preparation method possesses active significance in quantitative determination of sST2.

The technical solution of the present invention discloses a method for manufacturing an eye orbital and conjunctival sac growth stimulation device, including: obtaining the conjunctival sac and orbital shape data of the affected side; reconstructing the three-dimensional model data of the affected eye orbit based at least on the affected side's conjunctival sac and orbital shape data ; importing the three-dimensional model data of the affected side orbit into a three-dimensional printer; using materials with biocompatibility and plasticity to print out the orbit and conjunctival sac growth stimulating device through the three-dimensional printer. The technical scheme of the invention is simple and safe, does not require surgical implantation, is convenient to wear, and the patient feels comfortable after wearing it, without obvious adverse reactions, can fully stimulate the growth of the orbit and conjunctival sac, and has good cosmetic effect.

The invention provides a magnetic particle chemiluminescence detection kit for growth stimulation expression gene 2 protein, and belongs to the technical field of kits. The kit provided by the invention comprises a magnetic particle reagent and an acridinium ester reagent, the magnetic particle reagent comprises an ST2 antibody 1 coated with magnetic beads; the ST2 antibody 1 is purchased from Beijing holmes Biotechnology Co., Ltd., and the product number is HM148A-1148E; the acridinium ester reagent comprises an ST2 antibody 2 marked by acridinium ester; the ST2 antibody 2 is purchased from Beijing holmes Biotechnology Co., Ltd., and the product number of the ST2 antibody 2 is HM148A-1148F; the particle size of the magnetic beads is 1-3 microns, and carboxyl active groups are arranged on the surfaces of the magnetic beads. The magnetic particle chemiluminescence immunoassay kit for the growth stimulation expression gene 2 protein provided by the invention is high in sensitivity, good in specificity, high in precision, short in reaction time and simple to operate, and does not need to add a catalyst.

The invention provides a soluble growth stimulation expression gene 2 protein determination kit. The kit comprises a box body, and a cover body is hinged to one side of the box body. A first cavity, asecond cavity and a third cavity are sequentially formed in the box body. A centrifugal unit is mounted in the first cavity. A sterilized cotton bag placing area and a reagent card placing area are arranged in the second cavity, and a plurality of uniformly distributed partition plates are arranged in the reagent card placing area. A baffle and a placing plate are arranged in the third cavity, the baffle is positioned below the placing plate, a plurality of uniformly distributed through holes are formed in the baffle, a cooling area is formed in the third cavity below the baffle, a drawer boxis arranged in the cooling area, an ice bag is placed in the drawer box, a tool plate is further placed in the third cavity above the placing plate, tweezers, a sampling pipe and a suction head are placed on the tool plate, inserting blocks are arranged at the two ends of the bottom of the tool plate, and inserting grooves corresponding to the inserting blocks are formed in the side wall of the third cavity. The kit is simple to operate and can be used for rapidly detecting the soluble growth stimulating expression gene 2 protein.

The invention discloses a biological additive for livestock and poultry feed. The biological additive is prepared from the following components: a bacillus subtilis solution, a saccharomycetes solution, a lactic acid bacterium solution, an acetic acid bacterium solution, a saccharomyces cerevisiae solution and a plant lactobacillus solution in weight ratio of (0.4-1):1:(0.6-1.5):(0.6-1.5):(0.6-1.5):(0.3-0.7). After the biological additive for the livestock and poultry feed disclosed by the invention is infiltrated with feed and drinking water and enters the intestinal tracts of the livestock and poultry, free dominant populations can be formed by the biological additive infiltrated with the feed and drinking water, and beneficial florae in the intestinal tracts to inhibit and eliminate harmful pathogenic bacteria. Meanwhile, a big amount of amino acids, protein, vitamins, biochemical enzymes, antibiotics, growth promoting factors and other nutritional substances are secreted and synthesized, thereby adjusting and improving the organ functions of the bodies of the livestock and poultry, improving the feed conversion ratio, generating immunization, nourishment, growth stimulation andother effects on the livestock and poultry, and achieving the effects of eliminating odor of feces and urine, preventing and treating diseases, improving the survival rate, promoting growth and reproduction, purifying environments, improving economic benefits and the like.