The invention provides a soluble
growth stimulation expression gene 2
protein determination kit, a preparation method and application, and belongs to the technical field of biochemical
immunoassay. The latex enhanced
immunoturbidimetry is adopted to detect the soluble
growth stimulation expression gene 2
protein, key components of the
reagent 1 and the
reagent 2 are optimized and adjusted, preferred components and concentrations of polyanions, a surfactant, an
accelerant, a biochemical cross-linking agent and the like are obtained, and the problems that sST2 related reagents are poor in stability and poor in stability are solved. In order to solve the technical problems that in the prior art, the detection accuracy of the high value of sST2 larger than 400 ng / mL cannot be met and the like, the detection accuracy of the high value can be remarkably improved by the optimized components and concentration in the
linear range of 5 ng / mL to 432 ng / mL, particularly, the sensitivity, the anti-interference capability, the stability, the
repeatability and the front zone of the correspondingly prepared sST2 latex
reagent reach and are superior to those of a third-party reagent, and the detection accuracy of the high value of sST2 larger than 400 ng / mL can be remarkably improved by the optimized components and concentration in the
linear range of 5 ng / mL to 432 ng / mL. And the requirements of clinical use can be well met.