41 results about "Growth stimulation" patented technology

The invention relates to apparatus and method for ultrasonically stimulating cartilage growth. The apparatus includes at least one ergonomically constructed ultrasonic transducer configured to cooperate with a placement module or strip for placement in proximity to an area where cartilage growth is desired. The apparatus also utilizes a portable, ergonomically constructed main operating unit constructed to fit within a pouch worn by the patient. In operation, at least one ultrasonic transducer positioned in proximity to an osteochondral site is excited for a predetermined period of time. To ensure that at least one ultrasonic transducer is properly positioned, and to insure compliance with a treatment protocol, a safety interlock is provided to prevent inadvertent excitation of the at least one ultrasonic transducer.

The invention discloses a breeding method for medicinal silky fowl. The breeding method mainly comprises the steps of constructing a henhouse, brooding, breeding, disinfecting the henhouse and the like. The survival rate of chicken is greatly increased by adopting the breeding method provided by the invention for breeding silky fowl. According to the breeding method provided by the invention, a microbial technology is effectively combined with an ecological breeding technology, so that a powerful dominant bacterial community is formed in vitro and in vivo of animals under the effects of probiotic stock solution, fodder, drinking water and spraying sanitization, and a pathogenic bacteria colony is restrained and eliminated; a large quantity of nutrition and hormone matters, such as amino acid, protein, vitamin, various bio-chemical enzymes, antibiotics and somatomedin, are secreted and compounded, so that all the organ functions of animal bodies are adjusted and promoted; the feed conversion rate is increased; various functions, such as immunization, nutrition and growth stimulation are generated for the animals; obvious effects, such as preventing and curing diseases, increasing survival rate, promoting growth and breeding, lowering cost, eliminating fecaluria smell, purifying environment and increasing yield and income are achieved.

Bone growth for fusion promotion is stimulated in a mammalian patient in need thereof. Bone growth stimulation is achieved by implanting an electro-conductive bone growth stimulating implant in a region in the patient where bone growth is desired. An external device is worn by the patient to produce a direct current in the implant whereby bone growth is stimulated. The external device produces a magnetic field that induces an electric current in the implant. The electric current stimulates bone growth. The implant contains strips of a biocompatible conductive metal, such as, for example, nickel, gold or titanium. The strips can also be made of a conductive polymer such as for example, graphene. Implants to treat spinal cord pain are also disclosed.

A stem-cell-seeded porous scaffold implant and electromagnetic growth stimulation for treating or augmenting a breast tissue defect in a patient.

Fucose galactose carbohydrates have been shown to induce neuronal outgrowth. The invention includes methods of inducing neuronal outgrowth using carbohydrates, assemblies, and polymers bearing fucose-galactose moieties, as well as associated proteins. Cell growth can be stimulated in cells in culture or in cells within an animal or patient. Growth stimulation has application to understanding and treatment of neurodegenerative diseases including, for example, Parkinson's disease, Alzheimer's disease and multiple sclerosis and conditions such as stroke, brain injury and spinal cord injury. Such compounds, polymers, and assemblies also can be used to increase neural stem or progenitor cells in culture or in an animal, and to enervate engineered tissue.

The invention discloses an early rice seedling cold-proof agent and a using method thereof. The early rice seedling cold-proof agent is a solution formed by mixing, by weight, 30-40 parts of glucose, 20-30 parts of allantoin, 6-10 parts of aspirin, 0.3-0.5 part of gibberellin and 500 parts of water. The early rice seedling cold-proof agent has the advantages that the concentration of cell sap can be improved through the glucose, the cold hardiness of the cells can be improved through the allantoin, the aspirin has the antibacterial function, and the gibberellin has the growth stimulation function. After scientific compatibility of medicines, the cold resistance capacity of early rice seedlings can be improved, and the seedlings can be better promoted to grow strong.

The invention discloses a method for rapidly application of an esteya vermicola solid culture product in prevention and treatment of a pine wilt disease, and belongs to the technical field of biological control. According to the method, a certain proportion of nutrient substances, a protective agent, a growth stimulating factor, a spore stimulation factor and an adhesive are added into the solid culture product and are uniformly mixed, then a harmless plasticizer is added into the solid culture product, the solid culture product is pressed and processed into a long-rod-shaped product, finallya layer of wafer is wrapped around the surface of the long-rod-shaped product, the whole production process is isolated from the outside environment, manual operation is not needed, and therefore pollution caused by human factors or exposure to the external environment is reduced; during application, only two operation links of drilling and knocking-in are needed, a high-pressure plug does not need to be used, and therefore manpower and material resources are greatly reduced; meanwhile, the storage life of the product is greatly prolonged, and the biological activity of the product is remarkably improved; and the problem that a solid culture product cannot be directly applied to biological control can be solved. The method has the advantages of being simple in operation and low in cost when being used for the prevention and treatment work of woodland.

The invention discloses an industrialized production method for high galactosylated modification recombination human erythrocyte growth stimulation protein utilizing serum-free medium used for steadily improving protein expression index of Darbepoetin Alpha. The method comprises the following steps: adopting serum-free medium to cultivate CHO cell strain in a suspending manner in a biological reactor; expressing the Darbepoetin Alpha in high efficiency in the CHO cell. The invention further discloses the serum-free medium adopted by the industrialized production method for high galactosylated modification recombination human erythrocyte growth stimulation protein. The serum-free medium component used for steadily improving protein expression index of Darbepoetin Alpha is utilized; meanwhile, the CHO cell can efficiently and steadily express Darbepoetin Alpha; expression is stable; cultivation method is brief, and is suitable for large scale cultivation.

The invention relates to a growth stimulus expression factor 2 instant detection kit and a preparation method and application thereof, and the instant detection kit comprises an alkaline phosphatase labeled anti-growth stimulus expression factor 2 antibody working solution, a hydroxyl magnetic bead coated anti-growth stimulus expression factor 2 antibody working solution, a luminescent substrate working solution and a working cleaning solution; the luminous substrate working solution comprises a substrate solvent and 0.5% bovine serum albumin; the substrate solvent comprises the following components: 40% of diethanol amine, 30% of a 1M sodium hydroxide solution, 10% of a 0.1 M magnesium chloride solution, 10% of a 0.25% Proclin300 solution, 5% of a 0.5% gemini surfactant solution and 5% of AMPPD. The surface arrangement of the gemini surfactant solution is tighter, the surface energy is lower, the gemini surfactant solution has higher surface activity, the solubility of AMPPD in distilled water can be improved, the chemiluminescence reaction efficiency is promoted, and lower critical micelle concentration is achieved. The bovine serum albumin and the gemini surfactant are matched for use, so that the luminosity can be remarkably enhanced, and the detection sensitivity is further remarkably enhanced.

The invention relates to a method for automatically constructing a stable electrocatalytic bacterial biofilm. The method comprises the following steps: firstly assembling a microbe electrochemical reactor, and connecting circuits, wherein the first circuit is as follows: a fixed resistor is connected between an anode and a cathode in series, and the second circuit is as follows: an adjustable direct-current power supply, an ampere meter and a work contact of a circulating time relay are connected between the cathode and the anode; then injecting a bacteria growth medium solution into an anode chamber, inoculating a bacteria source, prestarting a microbe anode by switching on the first circuit, then controlling pulse current between the anode and the cathode to be gradually increased at preset velocity according to the potential change of the anode by switching on the adjustable direct-current power supply of the second circuit, wherein the pulse current is immediately lowered by 40%-60% when the potential of the anode is increased to a limit value and enters a new round of growth stimulation, and the electrocatalytic bacterial biofilm is completely constructed till the pulse current value reaches a preset current limit value and is stable at the preset current limit value for more than 10 hours. The electrocatalytic bacterial biofilm disclosed by the invention can be stably operated with the relatively high working current.

The invention relates to a preparation method of a poultry embryo egg extracting solution, and belongs to the technical field of health-care foods. The preparation method comprises the following technology steps of selecting poultry fertilized eggs, preforming conventional surface cleaning treatment, and performing conventional incubation for 8-12 days; after poultry embryos are determined to grow normally, performing cleaning, and disinfecting the surfaces of eggshells; collecting all substances in the embryos and eggs, and performing crushing with a crusher for 1-5 minutes so as to obtain homogenate; adding cane sugar which is 20-50% of the homogenate to the crushed homogenate, enabling the cane sugar to dissolve, and performing transferring to an ice bath; performing crushing treatment on the homogenate, wherein the crushing treatment comprises ultrasonic treatment, French high-pressure extruding treatment and/or grinding treatment with glass beads; performing centrifugation treatment on the homogenate; subpacking supernatant obtained through centrifugation in a sealed container, and performing pasteurization; or performing separation treatment on the supernatant obtained through centrifugation by a chromatographic fractionation method to obtain different particular protein and performing subpacking; and performing preservation at 4 DEG C or minus 20 DEG C. According to the preparation method disclosed by the invention, high-activity growth stimulation factors, high-activity nerve growth factors, high-activity active polysaccharide and high-activity active polypeptide are extracted and reserved to the maximum extent.

The invention discloses a Bacillus spp culture medium with coconut water as a main raw material. The culture medium comprises: (1) a growth stimulation culture medium (/L); (2) a liquid amplification culture medium (/L); and (3) a colony counting culture medium (/L). According to the invention, economical, cheap and widely available coconut water is used as the main raw material for the Bacillus spp culture medium, and a scientific and reasonable application method of the Bacillus spp culture medium is constructed at the same time; with the application method, the culture medium can meet requirements of scale-up culture of Bacillus spp; a leftover of coconut processing in Hainan province, i.e., coconut water, is made full use of, the Bacillus spp culture medium with coconut water as the main raw material is created for the first time, a good cheap culture medium formula is provided for development of scale-up culture of Bacillus spp in the future, and the Bacillus spp culture medium has a good application prospect; an effective approach is provided for high-valued utilization of coconut water, an added value of coconut processing can be obviously increased, and pollution of the industry of coconut processing to the environment can be reduced at the same time.

The technical solution of the present invention discloses a method for manufacturing an eye orbital and conjunctival sac growth stimulation device, including: obtaining the conjunctival sac and orbital shape data of the affected side; reconstructing the three-dimensional model data of the affected eye orbit based at least on the affected side's conjunctival sac and orbital shape data ; importing the three-dimensional model data of the affected side orbit into a three-dimensional printer; using materials with biocompatibility and plasticity to print out the orbit and conjunctival sac growth stimulating device through the three-dimensional printer. The technical scheme of the invention is simple and safe, does not require surgical implantation, is convenient to wear, and the patient feels comfortable after wearing it, without obvious adverse reactions, can fully stimulate the growth of the orbit and conjunctival sac, and has good cosmetic effect.

The invention provides a magnetic particle chemiluminescence detection kit for growth stimulation expression gene 2 protein, and belongs to the technical field of kits. The kit provided by the invention comprises a magnetic particle reagent and an acridinium ester reagent, the magnetic particle reagent comprises an ST2 antibody 1 coated with magnetic beads; the ST2 antibody 1 is purchased from Beijing holmes Biotechnology Co., Ltd., and the product number is HM148A-1148E; the acridinium ester reagent comprises an ST2 antibody 2 marked by acridinium ester; the ST2 antibody 2 is purchased from Beijing holmes Biotechnology Co., Ltd., and the product number of the ST2 antibody 2 is HM148A-1148F; the particle size of the magnetic beads is 1-3 microns, and carboxyl active groups are arranged on the surfaces of the magnetic beads. The magnetic particle chemiluminescence immunoassay kit for the growth stimulation expression gene 2 protein provided by the invention is high in sensitivity, good in specificity, high in precision, short in reaction time and simple to operate, and does not need to add a catalyst.

The invention provides a soluble growth stimulation expression gene 2 protein determination kit. The kit comprises a box body, and a cover body is hinged to one side of the box body. A first cavity, asecond cavity and a third cavity are sequentially formed in the box body. A centrifugal unit is mounted in the first cavity. A sterilized cotton bag placing area and a reagent card placing area are arranged in the second cavity, and a plurality of uniformly distributed partition plates are arranged in the reagent card placing area. A baffle and a placing plate are arranged in the third cavity, the baffle is positioned below the placing plate, a plurality of uniformly distributed through holes are formed in the baffle, a cooling area is formed in the third cavity below the baffle, a drawer boxis arranged in the cooling area, an ice bag is placed in the drawer box, a tool plate is further placed in the third cavity above the placing plate, tweezers, a sampling pipe and a suction head are placed on the tool plate, inserting blocks are arranged at the two ends of the bottom of the tool plate, and inserting grooves corresponding to the inserting blocks are formed in the side wall of the third cavity. The kit is simple to operate and can be used for rapidly detecting the soluble growth stimulating expression gene 2 protein.

The invention discloses a biological additive for livestock and poultry feed. The biological additive is prepared from the following components: a bacillus subtilis solution, a saccharomycetes solution, a lactic acid bacterium solution, an acetic acid bacterium solution, a saccharomyces cerevisiae solution and a plant lactobacillus solution in weight ratio of (0.4-1):1:(0.6-1.5):(0.6-1.5):(0.6-1.5):(0.3-0.7). After the biological additive for the livestock and poultry feed disclosed by the invention is infiltrated with feed and drinking water and enters the intestinal tracts of the livestock and poultry, free dominant populations can be formed by the biological additive infiltrated with the feed and drinking water, and beneficial florae in the intestinal tracts to inhibit and eliminate harmful pathogenic bacteria. Meanwhile, a big amount of amino acids, protein, vitamins, biochemical enzymes, antibiotics, growth promoting factors and other nutritional substances are secreted and synthesized, thereby adjusting and improving the organ functions of the bodies of the livestock and poultry, improving the feed conversion ratio, generating immunization, nourishment, growth stimulation andother effects on the livestock and poultry, and achieving the effects of eliminating odor of feces and urine, preventing and treating diseases, improving the survival rate, promoting growth and reproduction, purifying environments, improving economic benefits and the like.

The present invention relates to compositions and methods for identifying transformed cells. The method comprises introducing a visual marker polynucleotide into a plant cell and providing a growth stimulation protein. The compositions comprise a visual marker polynucleotide and a growth stimulation polynucleotide. Also provided are expression cassettes, plant cells, plant parts, and plants comprising same.