23 results about "Hindbrain" patented technology

The invention provides hindbrain organoids and methods of producing such organoids. Said hindbrain organoids may include healthy or healthy and diseased tissue. Also provided are methods of testing therapeutic agents using said hindbrain organoids. The invention also provides culture mediums used for producing (patterning) said hindbrain organoids and kits including the culture medias.

The invention discloses an adjustable maxillofacial region pressurizing and fixing device which comprises a longitudinal bandage, a transverse bandage and a lower jaw bandage. The longitudinal bandage is longitudinally wound on the head and detachably connected with the transverse bandage through the quadrilateral lock catch, and the transverse bandage is transversely wound on the forehead and the afterbrain; a band body of the longitudinal binding band is provided with a fixing device used for pressurizing the anterior parotid gland area of the ear, and the fixing device is in an oval shape and internally provided with a concave face. The longitudinal binding band is connected with the lower jaw binding band through the chin lock catch, the chin lock catch is tightly attached to the lower jaw, the lower jaw binding band is transversely wound on the lower jaw, and the lower jaw binding band is provided with the chin lock catch. The lock catches can be adjusted, medical pressurizing and fixing parts can be adjusted according to different surgical parts, and the problems that the head and the face are inconvenient to fix in the early stage and a traditional bandage is abraded are solved; a pressurizing fixing device is adopted and can be properly adjusted up and down, and a gauze roll can be placed in a concave surface to adjust the pressure; and the design of the chin lock catch and the chin lock catch is adopted, so that the whole body is more fit with the human face.

The present invention refers an in vitro method for inducing differentiation of human pluripotent stem cells (hPSCs) generating hindbrain neurons and compositions thereof. The invention also refers to the hindbrain neurons and compositions thereof for use in diagnostic screening and for use in the prevention and / or treatment of neurological disorders.

The application discloses a method for differentiating stem cells into caudal serotonergic neurons, a complete culture medium and application. Specifically, a new culture medium is formed by adding small molecule compounds, so that human pluripotent stem cells are gradually induced to obtain hindbrain neural stem cells, ventral hindbrain caudal neural stem cells, serotonergic precursor cells and caudal serotonergic neurons of the hindbrain. The culture method is simple, and can efficiently obtain mature serotonergic neurons of specific regions, thereby providing an effective cell model for research on diseases related to the serotonergic system.

Provided herein are methods of generating hindbrain cells, including respiratory hindbrain cells, from pluripotent stem cells. Also provided are methods of generating a three-dimensional organoid comprising a population of hindbrain cells including a heterogeneous population of interneurons.

The application discloses application of an RB1 mutant in a neurodegenerative disease. The application finds through experiments that juvenile fish and adult heterozygotes of a zrb1-KO mutant zebrafish (2 bases are deleted at positions 67 and 68 of the 2nd exon of an rb1 gene) exhibit motor and memory learning dysfunction, exhibit increased post-mitotic neuron apoptosis in the hindbrain, and have the characteristics of a neurodegenerative disease, and therefore, the zrb1-KO mutant zebrafish can be used to construct an animal model of a neurodegenerative disease. In addition, the application also statistically analyzes the mutation frequency and mutation type of RB1 of a neurodegenerative disease patient, and verifies the pathogenicity of R621S and L819V mutations of RB1 in neuron apoptosis through experiments, thereby providing an effective approach for diagnosis or treatment of a neurodegenerative disease.

The presently disclosed subject matter provides for in vitro methods of inducing differentiation of stem cells into hindbrain progenitor cells, enteric neural crest lineage cells, as well as differentiated hindbrain lineage or enteric neural crest lineage cells obtained by such methods. The presently disclosed subject matter also provides for uses of such cells for preventing and / or treating enteric nervous system disorders (e.g, Hirschsprung's disease), and for screening compounds suitable for preventing and / or treating enteric nervous system disorders (e.g., Hirschsprung's disease).

This invention discloses a trapezius muscle acupressure massage device. The trapezius muscle acupressure massage device of this invention, through its back-of-the-head and cervical spine placement parts formed by closely following the curves of the user's back of the head and neck, allows the user's head to remain in a naturally fixed state. Therefore, the piston of the trapezius muscle acupressure module can apply accurate and stable acupressure to the trapezius muscle, thereby relaxing and strengthening the muscles around the trapezius muscle. This promotes and activates blood circulation, and further enhances the therapeutic effect through acupressure, traction, and stretching that promote metabolism.

The present application belongs to the technical field of sitting furniture, and particularly relates to a multifunctional sitting furniture for relieving cervical and lumbar vertebrae and a use method. The multifunctional sitting furniture for relieving cervical and lumbar vertebrae comprises a chair leg, a seat cushion is arranged on the chair leg, a backrest support is hingedly arranged at the rear side of the seat cushion, a multifunctional pillow is arranged on the backrest support and is in lifting and lowering activity connection with the backrest support, the multifunctional pillow is in close contact with the back of the head and the back of the brain when the multifunctional pillow is lifted to the rear side of the head relative to the backrest support, the top of the multifunctional pillow supports and abuts against the chin when the multifunctional pillow is lowered below the chin relative to the backrest support, a backrest is arranged on the backrest support and is below the multifunctional pillow and in lifting and lowering activity connection with the backrest support, and multifunctional cantilevers are arranged on both sides of the backrest and extend to the waist on both sides of the human body. The present application has the advantages that the pressure on the cervical and lumbar vertebrae is relieved.

The utility model relates to the technical field of clinical nursing of respiratory medicine. The tightness-adjustable oxygen inhalation mask comprises a main body structure, the main body structure comprises a breathing mask, two sets of first clamping discs, two sets of second clamping discs and two sets of springs, and the number of the first clamping discs, the number of the second clamping discs and the number of the springs are all two. The breathing mask and the head support are attached to the face and the afterbrain of a patient respectively, then the two sets of adjusting discs are pressed inwards to move, the two sets of second clamping discs are driven to move synchronously, and the two sets of second clamping discs and the two sets of first clamping discs are not clamped directly any more; then two groups of rotating handles are rotated to drive adjusting discs to rotate, two groups of bandages are wound on the outer surfaces of the two groups of adjusting discs respectively, so that the lengths of the two groups of bandages are shortened, and by means of the design, the equipment can be adjusted according to patients under different conditions during use, and the equipment can be better attached to the faces of the patients.

The invention discloses an electroacupuncture and PNF collaborative ACLR afterbrain limb integrated precise rehabilitation method and system, and relates to the technical field of clinical medicine rehabilitation treatment, and the method comprises the steps: S1, baseline function and iconography evaluation; s2, preparing center modulation; s3, peripheral motion driving is carried out; s4, quantitative acquisition and response evaluation; s5, performing parameter adjustment and periodic execution; according to the invention, quantified physiological and functional indexes are embedded as a closed-loop adjustment basis, so that the electroacupuncture parameters and PNF strength of each treatment can be optimized in a data-driven manner. Through periodical evaluation of clinical knee joint function scores, balance parameters, SEMG and central function activation and adjustment and intervention according to preset rules, the method realizes transformation from empirical rehabilitation to evidence-driven and individualized rehabilitation. According to the closed-loop design, benefits of individuals on intervention can be amplified, and adverse reactions or overload risks can be recognized in real time, so that the possibility of treatment side effects or function degradation is reduced.

The application provides a kind of gold pompano heat stress resistance cultivation method, using domestication pool I, II, III, according to juvenile stage, juvenile stage, adult fish stage, gold pompano is domesticated, in juvenile stage, using bait domestication, through the specific bait formula in nutrition or pharmacological means, the fat utilization ability of fish body can improve the heat stress tolerance of fish, temperature domestication is carried out in juvenile stage, four-stage temperature domestication is adopted, the heat tolerance of fish body is gradually trained, the defense ability of non-specific immunity of fish body is improved, sound wave domestication is carried out in adult fish stage, the sound information is transmitted to the motor-related neuron in hindbrain, so as to promote the tail movement of itself, improve the immune function of fish body, the application domesticates the feeding, enemy, circadian rhythm regulation ability of fish body, improves the physical fitness of fish body to enhance the heat reaction ability, reduces the probability of heat reaction, at the same time, can better, faster adapt to harsh breeding environment, the survival rate reaches 98.8% after stress test, and the weight loss rate is-0.02%.

The present disclosure relates to a method for preparing a cerebellar organoid, comprising: (A) a step of selective differentiation into hindbrain tissue, in which embryoid bodies formed from pluripotent stem cells are cultured in the presence of a hindbrain tissue induction medium including an endogenous WNT secretion inhibitor and a WNT signaling agonist; (B) a step of selective differentiation into dorsal metencephalic tissue, in which the embryoid bodies are cultured in the presence of a dorsal metencephalic tissue induction medium including a sonic hedgehog (SHh) signaling pathway inhibitor; and (C) a basal surface-apex surface polarization step of inducing basal surface-apex surface polarization, in which polarization of the embryoid bodies are induced in the presence of a basal surface-apex surface induction medium including an extracellular matrix-based hydrogel.

The utility model relates to the field of spine supporting protection, in particular to a spine external fixation supporting protection device which comprises a connecting block, a rotating limiting rod, a supporting rear plate, a jaw support assembly and a spine supporting assembly. The jaw support assembly comprises a support frame; a first adjusting wheel is mounted at the rear end part of the support frame; a first limiting bolt is arranged at the rear end part of the first adjusting wheel; the ends of the two sides of the supporting frame are rotationally connected with rotating supporting rods through rotating shafts. Rotating frames are arranged at the ends of the two sides of the first adjusting wheel. The head is positioned through the supporting frame and the lower jaw supporting plate, and the body of a patient is fixed by matching with the positioning column and the rotating limiting rod, so that the spine is fixed and corrected, and the problem that in the spine supporting and protecting process, due to the fact that most existing spine external fixing, supporting and protecting devices are provided with separated lower jaw and afterbrain positioning devices, the spine cannot be fixed and corrected is solved. When the spine is externally fixed and supported, the positioning between the spine and the afterbrain is relatively separated, so that the use effect of the spine external fixing, supporting and protecting device is easily reduced.

A brain magnetic detector support comprises a main support and at least one sub-support, the sub-support can be adjusted relative to the main support, the position of each sub-support relative to the main support can be adjusted according to needs, brain magnetic detector installation portions are formed on the main support and the sub-supports respectively, and the main support and the sub-supports are connected through the brain magnetic detector installation portions. The brain magnetic detector mounting portions are arranged in a predetermined pattern. The brain magnetic detector support is provided and comprises a main support body covering the head top portion, a first sub-support body and a second sub-support body, and the first sub-support body and the second sub-support body can be adjusted or moved relative to the main support body to be attached to the cheeks and the afterbrain. During wearing, the first sub-support and the second sub-support are moved away from the main support, so that the whole support can be easily worn on the brain, and after wearing, the first sub-support and the second sub-support are adjusted to be close to cheeks and afterbrain, so that each brain magnetic detector borne on the main support and the sub-supports can be close to the head. Therefore, the measurement precision is improved.

The present disclosure provides a method of producing human sensory organoids in vitro. The present disclosure provides a method of producing human dorsal hindbrain / cervical spinal cord organoids in vitro. The present disclosure provides a method of producing human ascending somatosensory assembloids in vitro. The present disclosure also provides a method of determining the effectiveness of a candidate agent on a human ascending somatosensory pathway assembloid. Also provided are human sensory organoids, human dorsal hindbrain / cervical spinal cord organoids, and human ascending somatosensory assembloids.

The invention provides a wearable brain-computer interface computer and an interaction control method thereof, and relates to the technical field of brain-computer interfaces. The wearable device comprises an anterior brain wearable part and an afterbrain wearable part, and the front end face of the afterbrain wearable part is fixedly connected with a fixed connecting rod; the tail end of the fixed connecting rod is sleeved with an adjusting pipe, and a first connecting ring is fixedly welded to the outer portion of the fixed connecting rod. And the outer part of the adjusting pipe is sleeved with an adjusting belt. After the forebrain wearing part and the afterbrain wearing part are assembled, electroencephalogram signals generated by the forebrain cortex and the afterbrain cortex are relatively more complete, the signal collection accuracy is improved, after the distance between the forebrain wearing part and the afterbrain wearing part is adjusted in the front-back direction of the adjusting belt, the wearable equipment is suitable for users with different head circumferences, and therefore the customization number can be reduced, the cost is reduced, and the user experience is improved. And the problems that most brain-computer interface equipment needs to be customized, the use cost is high, and market expansion is not facilitated are solved.

The invention discloses a neck fixing device for the emergency department. The neck fixing device comprises a supporting frame, a lower jaw bearing part, a side supporting assembly and an afterbrain bearing part. The lower jaw bearing part is adjustably arranged on the front side of the supporting frame; the side support assembly comprises a first side support and a second side support which correspond to the two sides of the neck, the first side support and the second side support are arranged on the left side and the right side of the supporting frame in an independent lifting adjusting mode respectively, and side adjusting pieces for driving the first side support and the second side support are further arranged on the two sides of the supporting frame. The afterbrain bearing part is adjustably arranged on the rear side of the supporting frame. The technical scheme has the beneficial effects that the first side support and the second side support can be independently adjusted so as to fix a patient at an optimal position, and particularly, when the neck of the patient is injured due to an emergency, the head of the patient cannot be recovered to an anatomical position under the condition, so that the patient cannot be injured. The neck and the head of the patient are fixed to the current position through independent adjustment of the first side support and the second side support, and secondary injury is avoided.

The purpose of the present invention is to provide a method whereby hindbrain neural stem cells can be cultured over a long period of time while maintaining multipotency. The present invention relates to a method for manufacturing hindbrain neural stem cells, the method involving: a step for inducing differentiation of hindbrain neural stem cells from pluripotent stem cells; and a step for culturing the hindbrain neural stem cells by using a culture medium that contains a GSK3β inhibitor, a TGFβ inhibitor, and a BMP inhibitor and does not contain a basic fibroblast growth factor (bFGF).

The utility model discloses an adjustable height's pillow relates to pillow technical field, including pillow support, and the pillow support is divided out with human body pillow pad part corresponding neck support area, left side support area, right side support area and back brain support area, further, be equipped with neck support height adjusting unit for adjusting neck support height on neck support area, be equipped with side support height adjusting unit for adjusting side support height on left side support area and right side support area, be equipped with back brain pillow support height adjusting unit for adjusting back brain pillow support height on back brain support area. Through setting up corresponding adjusting unit, make every support area can realize individual height adjustment, satisfy the use demand of user, ensure when using the pillow, cervical vertebra is in the most comfortable state, effectively avoid the harm to cervical vertebra because of the discomfort of pillow height.

The invention belongs to the technical field of biological pharmacy, and provides application of glycyrrhizin in preparation of a medicine for treating white matter injury. Animal experiment results show that the HMGB1 inhibitor Gly can obviously improve rat WMI pathological changes, reduce rat brain white matter region HMGB1 expression, obviously reduce the number of rat brain white matter region NG2 + Olig2 + double positive cells and improve rat WMI afterbrain white matter region OLs differentiation disorder; the myelination disorder of the white matter region of the rat after WMI can be improved, and the number of myelination axons of the rat is obviously increased; and the learning ability and the spatial memory ability of the rats are improved. It is clear that Gly can inhibit expression of HMGB1, and an effective technical means is provided for treatment of white matter damage.

The invention relates to the technical field of spinal external fixation braces, in particular to a novel spinal external fixation brace which comprises a brace front chest plate, an elastic steel plate and a neck supporting assembly, a crossed chest strap is hinged to the inner side of the brace front chest plate, and a brace rear waist plate is hinged to the back side end of the crossed chest strap; a transverse fixing belt is hinged to the inner side of the crossed chest belt, a length adjusting buckle is arranged at the outer side end of the crossed chest belt, a lower jaw supporting plate is arranged above the crossed chest belt, afterbrain supporting plates are symmetrically arranged on the side of the lower jaw supporting plate, and a neck supporting assembly is arranged outside the lower jaw supporting plate and the afterbrain supporting plates. According to the fixing brace, the feeding through opening is specially formed in the lower jaw supporting plate in the fixing brace, and the opening and closing design of the magnetic attraction lap joint belt and the magnetic attraction butt joint belt is adopted, so that a patient can normally open the mouth for feeding without detaching the neck support, the movement of the lower jaw can be properly recovered only by loosening the magnetic attraction lap joint belt, and the mouth opening requirement of the patient is met.

The present disclosure relates to a method for selective differentiation from pluripotent stem cells to hindbrain tissue, the method comprising a step of selective differentiation into hindbrain tissue, in which embryoid bodies formed from pluripotent stem cells are cultured in the presence of a hindbrain tissue induction medium containing endogenous WNT secretion inhibitors and WNT signaling agonists.