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Vaccine for preventing red head disease of Pseudobagrus fulvidraco

A vaccine and a technology for fish, applied in the field of vaccines to prevent yellow catfish red head disease, can solve the problems of aquatic product quality impact, economic loss, and increase in treatment costs, and achieve good results and low cost

Active Publication Date: 2010-09-15
TONGWEI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This new type of vaccination helps protect fish from harm caused by parasites that cause it sicknesses like Yellow Cat Fish Red Head (YC). It works well with people who are already infected but have no symptoms for them because they don't develop any signs until after being bitten down through their eyes on these seafood.

Problems solved by technology

This patented technical problem addressed in this patents relates to improving methods for preventing or treating diseases caused by harmful algae called pink crabs that can cause severe damage when eaten raw food containing these organisms. Current therapies involve administering chemical agents such as chlorine gas or nitrogen oxide into affected parts of crops where they may be causing further damaging symptoms like blobbings at their eyes.

Method used

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  • Vaccine for preventing red head disease of Pseudobagrus fulvidraco
  • Vaccine for preventing red head disease of Pseudobagrus fulvidraco
  • Vaccine for preventing red head disease of Pseudobagrus fulvidraco

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment one pathogenic bacteria Isolation and Identification of Edwardsiella ictaluri

[0025] 1 Materials and methods

[0026] 1.1 Test fish

[0027] Naturally occurring yellow catfish was collected from the Meishan yellow catfish farm in Sichuan; healthy yellow catfish was provided by Tongwei Co., Ltd. Sichuan Company, weighing 60.5±4.3g.

[0028] 1.2 Main reagents

[0029] Medium: TSA, TSB, TIS and BHI are self-made according to conventional methods.

[0030] Bacterial biochemical trace identification tube: purchased from Hangzhou Tianhe Biological Reagent Co., Ltd.

[0031] Taq DNA polymerase: purchased from Dalian Bao Biological Company.

[0032] PCR primers: synthesized by Dalian Bao Biological Company.

[0033] Drug-sensitive paper: purchased from Hangzhou Tianhe Biological Reagent Co., Ltd.

[0034] 1.3 Isolation and identification of bacteria

[0035] 1.3.1 Isolation of pathogenic bacteria: Sampling the brain and kidney of naturally infected fish by...

Embodiment 2

[0067] Embodiment two Comparative Study on the Standard Strain of Edwardsiella ictaluri and the CHNGY01 Strain Isolated by the Present Invention

[0068] 1 Materials and methods

[0069] 1.1 Source of bacteria

[0070] Strain CHNGY01 was isolated and identified from Tongwei Institute of Pharmaceutical Sciences from a yellow catfish farm in Meishan, Sichuan Edwardsiella, the deposit number is CCTCC NO: M209176.

[0071] The standard strain of Edwardsiella is ATCC 33202.

[0072] 1.2 Main reagents:

[0073] Bacterial biochemical trace identification tube: purchased from Hangzhou Tianhe Microbiological Reagent Co., Ltd.

[0074] Medium: Brain Heart Infusion (BHI) was purchased from Beijing Luqiao Technology Co., Ltd.

[0075] 1.3 Bacteria solution preparation

[0076] Before the test, use an inoculation loop to take the bacterial liquid from the glycerol bacterial liquid of the preserved strains, inoculate it in the brain heart infusion (BHI) broth medium, incubate at ...

Embodiment 3

[0087] Bacterial strain CHNGY01 of the present invention and Comparative Study on Inactivated Whole Bacteria Prepared by Edwardsiella Standard Strain (ATCC33202)

[0088] The "red head disease" of yellow catfish is a serious infectious disease that has occurred in recent years. The previous research has confirmed that its pathogen is Edwardsiella. In this experiment, the separation The comparative study on the immune effect of the inactivated vaccine prepared by Edwardsiella and the standard strain ATCC33202 on the red-head disease of yellow catfish provides a reliable basis for better immune prevention and treatment of red-head disease of yellow catfish.

[0089] 1. Materials and methods

[0090] 1.1 Strains

[0091] Yellow catfish Isolation and identification of Edwardsiella (CHNGY01) from yellow catfish naturally infected with red head disease

[0092] Edwardsiella Standard Strain (ATCC33202) ATCC Corporation

[0093] 1.2 Experimental fish

[0094]Healthy yell...

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Abstract

The invention provides application of Edwardsiella ictaluri to the preparation of fish vaccines and a fish vaccine. The fish vaccine comprises effective dose of immunogenic inactivated Edwardsiella ictaluri and pharmaceutically acceptable auxiliary materials or carriers. The vaccine or combined vaccine of the invention can pertinently prevent and treat red head disease of Pseudobagrus fulvidraco with good effect and is a low-cost and environmental-friendly prevention method.

Description

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Claims

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Application Information

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Owner TONGWEI
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