37 results about "Edwardsiella ictaluri" patented technology

Aeromonas hydrophila is a reemerging pathogen of channel catfish (Ictalurus punctatus); recent outbreaks from 2009 to 2014 have caused the loss of more than 12 million pounds of market size catfish in Alabama and Mississippi. Genome sequencing revealed a clonal group of A. hydrophila isolates with unique genetic and phenotypic features that is highly pathogenic in channel catfish. Comparison of the genome sequence of a representative catfish isolate (ML09-119) from this virulent clonal group with lower virulence A. hydrophila isolates revealed four fimbrial proteins unique to strain ML09-119. In this work, we expressed and purified four A. hydrophila fimbrial proteins (FimA, Fim, MrfG, and FimOM) and assessed their ability to protect and stimulate protective immunity in channel catfish fingerlings against A. hydrophila ML09-119 infection for vaccine development. Our results showed catfish immunized with FimA, Fim, FimMrfG, and FimOM exhibited 59.83%, 95.41%, 85.72%, and 75.01% relative percent survival, respectively, after challenge with A. hydrophila strain ML09-119. Bacterial concentrations in liver, spleen, and anterior kidney were significantly (p<0.05) lower in vaccinated fish compared to the non-vaccinated sham groups at 48 h post-infection. However, only the Fim immunized group showed a significantly higher antibody titer in comparison to the non-vaccinated treatment group (p<0.05) at 21 days post-vaccination. Altogether, Fim and FimMrfG recombinant proteins have potential for vaccine development against virulent A. hydrophila infection. Genomic subtraction revealed three outer membrane proteins present in strain ML09-119 but not in the low virulence reference A. hydrophila strain; the major outer membrane protein OmpAI (OmpA1), TonB-dependent receptor (TonB-DR), and transferrin-binding protein A (TbpA). Here, the genes encoding OmpAI, tonB-DR, and tbpA were cloned from A. hydrophila ML09-119 and were expressed into Escherichia coli. The purified recombinant OmpA, TonB-DR, and TbpA proteins had estimated molecular weights of 37.26, 78.55, and 41.67 kDa, respectively. Catfish fingerlings vaccinated with OmpA1, TonB-DR, and TbpA emulsified with non-mineral oil adjuvant were protected against the subsequent A. hydrophila ML09-119 infection with 98.59%, 95.59%, and 47.89% relative percent survival (RPS), respectively. Furthermore, the mean liver, spleen, and anterior kidney bacterial loads were significantly lower in catfish vaccinated with the OmpA1 and TonB-DR than the non-vaccinated control group. ELISA demonstrated that catfish immunized with OmpA1, TonB-DR, and TbpA produce significant antibody response by 21 days post-immunization. Therefore, data generated during the study suggest that OmpAI and TonB-DR proteins could be used as potential candidates for vaccine development against A. hydrophila epidemic strain infection. However, TbpA protein failed to provide such strong protection. Recombinant ATPase from A. hydrophila also showed promise as a vaccine antigen. A live attenuated vaccine was prepared that combined the advantages of a live attenuated vaccine (ESC-NDKL1 (ΔgcvPΔsdhCΔfrdA) mutant of Edwardsiella ictaluri) against enteric septicemia of catfish (ESC) and three immunogenic recombinant proteins (Fim, FimMrfg, and ATPase) against A. hydrophila infection. Our results showed channel catfish fingerlings immersion-vaccinated with ESC-NDKL1::pETfim, ESC-NDKL1::pETmrfG, and ESC-NDKL1::pETATPase exhibited 100%, 91.67%, and 100% percent survival after challenge with the A. hydrophila ML09-119, which was significantly less than non-vaccinated group (88.89% mortality). In a second study, Catfish immunized with NDKL1::pETfim, ESC-NDKL1::pETmrfG, ESC-NDKL1::pETATPase had significantly (p<0.05) lower mortalities than sham-vaccinated group.

The invention provides a kit for detecting fish pathogenic bacteria; the kit comprises a primer with a nucleotide sequence as shown in SEQ ID NO. 1-2, and the primer is used to amplify genes of edwardsiella ictaluri. The invention also provides a method for detecting fish pathogenic bacteria, and an application of the primer shown in SEQ ID NO. 1-2. The kit provided by the invention can rapidly and accurately detect a pathogenic bacterium of ictalurus punctatus - edwardsiella ictaluri, and thus realizes pertinent control and detection of ictalurus punctatus enteric septicemia, and the invention has wide application prospects.

The invention relates to the field of aquaculture, and discloses lactobacillus acidilactici, an aquatic feed additive based on the lactobacillus acidilactici, fish feed and application of the lactobacillus acidilactici, the aquatic feed additive and the fish feed. The lactobacillus acidilactici can promote the growth performance of cultured fishes, improve the immunity of the cultured fishes, especially improve the capacity of resisting Edwardsiella infection of longsnout catfish, well adapt to the digestive tract environment of the cultured fishes and stably play a role.

A novel Edwardsiella ictaluri E-ict-VL33 strain, a vaccine derived from the novel Edwardsiella ictaluri E-ict-VL33 strain, especially in immersion form and oral form, and a method for protecting fishes from the infection of Edwardsiella ictaluri, especially catfish, the method including a primary immersion immunization using immersion vaccine, and after an appropriate period, boosting by oral vaccination, resulting in a strong and long-lasting immunity for fishes.

A live attenuated Edwardsiella ictaluri bacterium lacking a viable gene encoding a functional evpB protein and a method of using the same to protect fish against infection from virulent Edwardsiella ictaluri. The methods and compositions for protecting fish against infection from virulent Edwardsiella ictaluri comprising administering to a fish a therapeutically effective amount of an attenuated Edwardsiella ictaluri bacterium lacking a viable gene encoding a functional EvpB protein. The bacterium may include an insertion and / or deletion mutation in the evpB gene. The fish include catfish, preferably catfish fingerling or a catfish fry. The composition may be delivered via immersion delivery, an injection delivery, an oral delivery, or combinations thereof.

The invention relates to the field of aquatic product vaccinology, in particular to a vibrio harveyi, vibrio anguillarum and Edwardsiella tarda triple inactivated vaccine, and preparation and application thereof. The triple inactivated vaccine contains inactivated vibrio harveyi T4, vibrio anguillarum C312 and Edwardsiella tarda TX1. The highest relative percent survival (RPS) of the obtained inactivated vaccine for the vibrio harveyi, the vibrio anguillarum and the Edwardsiella tarda can be respectively 89.29%, 85.71% and 76.67%, and the RPS still can be about 50% in six months after immunization is carried out. The obtained vaccine has a simple preparation process and a low construction cost, does not have potential safety hazards, has a good immunization effect and can prevent and control three kinds of different-species pathogen infection through one-time immune inoculation.

The present invention is directed to a novel live attenuated isolate and sub-isolets thereof of a strain of the pathogen Edwardsiella ictaluri for protecting fish from ESC infection or disease, a vaccine composition comprising the isolet, a method of oral delivery of the vaccine and any vaccine, and an apparatus designed to effectively mix the vaccine with fish feed and deliver the vaccine to fish.

Live attenuated bacteria vaccines against enteric septicemia of fish, especially catfish, and methods related to the same. Mutant strains of the bacteria Edwardsiella ictaluri (a pathogenic bacterial strain of Enterobacteriaceae) are provided. The mutant Edwardsiella ictaluri bacteria (or other pathogenic bacterial strain of Enterobacteriaceae) contain one or more gene deletions or disruptions that result in less virulent bacterial strains as live attenuated vaccine compositions against virulent wild-type Edwardsiella ictaluri bacteria (or other pathogenic bacterial strain of Enterobacteriaceae). The mutant strains showing the best immunological protection and safety as a vaccine are the triple mutants ESC-NDKL1 (ΔgcvPΔsdhCΔfrdA) strain and ESC-NDKL2 (ΔgcvPΔsdhCΔmdh) strain, with the ESC-NDKL1 strain providing the greatest safety and efficacy of these two triple mutants.

The invention provides application of Edwardsiella ictaluri to the preparation of fish vaccines and a fish vaccine. The fish vaccine comprises effective dose of immunogenic inactivated Edwardsiella ictaluri and pharmaceutically acceptable auxiliary materials or carriers. The vaccine or combined vaccine of the invention can pertinently prevent and treat red head disease of Pseudobagrus fulvidraco with good effect and is a low-cost and environmental-friendly prevention method.

The invention discloses an edwardsiella ictaluri colloidal gold immunochromatographic strip which can rapidly detect edwardsiella ictaluri in aquatic animals and a preparation method thereof. The edwardsiella ictaluri colloidal gold immunochromatographic strip comprises a bottom plate, a sample pad, a conjugate pad, a nitrocellulose membrane and a water absorption pad are sequentially stuck on the bottom plate from one end from top to bottom in a stepped manner, a colloidal gold-labeled edwardsiella ictaluri monoclonal antibody is sprayed on the conjugate pad, a detection line a coated with an edwardsiella ictaluri polyclonal antibody and a quality control line b constituted by goat anti-mouse IgG are arranged on the nitrocellulose membrane, and the edwardsiella ictaluri monoclonal antibody is prepared by immunizing a mouse with a hybridoma cell strain Ei-5D11 of an anti-edwardsiella ictaluri monoclonal antibody with the collection number of CGMCC No. 7305, obtaining ascites of the mouse, and purifying.

The invention discloses a medicine for preventing and treating Edwardsiella disease of eel and a preparation method thereof. The medicine formula is as follows: 1-5 parts of gall, 1-5 parts of skullcap, 1-5 parts of angelica, 5-15 parts of knotweed, and 5-15 parts of andrographis paniculata 10 to 50 parts of Hedyotis diffusa, 5 to 30 parts of Houttuynia cordata, 5 to 15 parts of Chickweed, 5 to 50 parts of purslane, and 5 to 30 parts of licorice. Toxin, hemostasis, Angelica dahurica for removing blood stasis and expelling pus, promoting muscle and relieving pain, Polygonum cuspidatum promoting blood circulation, dissipating blood stasis, Andrographis paniculata and Portulaca oleracea to detoxify, reduce inflammation and relieve pain, protect liver and protect liver, Hedyotis diffusa clears heat and detoxifies, relieves pain and disperses knots , Houttuynia cordata antibacterial, enhance immunity, chicken bone grass clearing heat and detoxification, soothing liver and relieving pain, licorice clearing heat and detoxification, invigorating spleen and nourishing qi, reconciling the effects of various medicines, can effectively eliminate eel pectoral and anal fin surface bleeding, liver and kidney enlargement , congestion, ulcers, gastrointestinal effusion and other diseases, with reasonable formula, fast onset, safe and non-toxic, stable efficacy and so on.

The invention provides a kit for detecting fish pathogenic bacteria; the kit comprises a primer with a nucleotide sequence as shown in SEQ ID NO. 1-2, and the primer is used to amplify genes of edwardsiella ictaluri. The invention also provides a method for detecting fish pathogenic bacteria, and an application of the primer shown in SEQ ID NO. 1-2. The kit provided by the invention can rapidly and accurately detect a pathogenic bacterium of ictalurus punctatus - edwardsiella ictaluri, and thus realizes pertinent control and detection of ictalurus punctatus enteric septicemia, and the invention has wide application prospects.

The invention belongs to the technical field of antibacterial agents for water environment, and discloses a compound plant extract for Edwardsiella tarda in water environment. The compound plant extract is mainly made of the following components in mass percentage: Extract 55%-65%, Portulaca oleracea extract 26%-35%, Jinbuchang stem extract 12%-18%. Compared with the safety of traditional GMP disinfectants, the compound plant extract of the present invention belongs to environment-friendly substances, and has a large safe concentration range for scaleless fish. When splashed with water, it has obvious inhibitory effect on Edwardsiella tarda, And it will not be disturbed by organic matter in the water body. When the dosage is ≤5mg / L, it can effectively inhibit the abnormal reproduction of Edwardsiella tarda in the upper, middle and bottom layers of the aquaculture water body, and it is effective against the yellow catfish, channel catfish and channel catfish. Security is good.