A compound plant extract for inhibiting Edwardsiella tarda in water environment and its preparation method and application
A technology of plant extracts of Edwardsiella tarda, which is applied in the field of compound plant extracts and its preparation for inhibiting Edwardsiella tarda in water environments, can solve the problems of increased risk of pathogenicity, low value, and high cost of use, and reach a safe concentration range Large, inhibition of abnormal reproduction, good inhibitory effect
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Embodiment 1
[0070] A compound plant extract for inhibiting Edwardsiella tarda in water environments, by weight percentage, weighed 56 parts of the fresh leaf extract of Z. In the environment of 28°C, all the components mentioned above can be mixed, and the obtained mixed liquid is the compound plant extract.
[0071] Wherein, above-mentioned purple back grass fresh leaf extract is obtained by following method:
[0072] (1) Mix the fresh leaves and salt according to the mass percentage (1 / 100) and fully grind them into mash, mix the mash with mature vinegar solution at a mass volume ratio of 1:10, and place at room temperature 33-35°C Airtightly mix in the environment for 8-10 days, keep stirring at intervals of 3-4 hours for 4-5 minutes during the period, and take out the fresh leaves of the vinegar-made purple backgrass;
[0073] (2) Mix the vinegar-made purple-backed grass fresh leaves with water, lactic acid bacteria liquid culture medium (the weight-to-volume ratio is 8:22:5) and lac...
experiment example 1
[0076] Experimental Example 1 Composite Plant Extract of the present invention compares the inhibitory effect of common aquatic pathogenic bacteria
[0077] 1. Test material
[0078] 1.1 Test strains
[0079] Edwardsiella tarda, Aeromonas hydrophila, and Flavobacterium columnar: all are preserved and provided by our research group.
[0080] Povidone-iodine: a 10% povidone-iodine solution.
[0081] 1.2 Test consumables
[0082] TSB medium, Petri dishes, test tubes, distilled water, etc.
[0083] 1.3 Compound plant extracts
[0084] The compound plant extract of the present invention used in this experiment, its preparation method is the same as embodiment 1.
[0085] 2. Test method
[0086] 2.1 Bacterial suspension preparation
[0087] Inoculate Edwardsiella tarda, Aeromonas hydrophila, and Flavobacterium columnar in TSB nutrient broth, culture at 30°C for 16-20 hours, collect the bacteria by centrifugation, and conduct turbidimetric estimation of the bacterial liquid th...
experiment example 2
[0104] Experimental example 2 The compound plant extract prescription compatibility of the present invention is to the inhibition of Edwardsiella tarda
[0105] 1. Test material
[0106] 1.1 Preparation of compound plant extracts
[0107] The composite plant extract of the present invention used in this experiment is named as A, and its preparation method is the same as in Example 1.
[0108] 1.2 Preparation of compound plant extracts with missing prescriptions
[0109]Based on the compound plant extract formula prepared in Example 1 of the present invention, through the technical idea of single-component deletion or double-component simultaneous deletion or three-component simultaneous deletion and the missing components are replaced by PBS in equal amounts to prepare The compound plant extract with single deletion of purple back grass fresh leaf extract, the single deletion compound plant extract of purslane extract, the single deletion compound plant extract of golden b...
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