A compound plant extract for inhibiting Edwardsiella tarda in water environment and its preparation method and application

A technology of plant extracts of Edwardsiella tarda, which is applied in the field of compound plant extracts and its preparation for inhibiting Edwardsiella tarda in water environments, can solve the problems of increased risk of pathogenicity, low value, and high cost of use, and reach a safe concentration range Large, inhibition of abnormal reproduction, good inhibitory effect

Active Publication Date: 2021-08-03
清远海贝生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In actual farming, in Guangdong, Hubei, Sichuan and other scale-free farming areas, a relatively common phenomenon is: when the water body temperature and organic matter and other conditions are suitable for the rapid propagation of E. tarda, E. tarda has a strong effect on channel catfish, channel catfish, catfish, The disease risk of yellow catfish is greatly increased. Once it enters the fish and causes disease, firstly, it will delay the effective feeding time, secondly, the cost of medicine will increase, and thirdly, the use of chemical medicine will lead to the risk of drug residue
[0004] By inhibiting the abnormal reproduction of Edwardsiella tarda in water bodies, thereby cutting off the risk of transmission of Edwardsiella tarda is one of the commonly used prevention and control methods. Commonly used disinfectants such as chlorine dioxide, povidone-iodine and potassium salts, although It has good safety and bactericidal ability, but it is easily affected by the content of organic matter in the water body. It is often difficult to achieve the bactericidal effect when using conventional doses when delayed Edwardsiella occurs. The total use cost of the 2-3 disinfection courses within is very high, which greatly reduces the willingness of farmers to use it; and in the research report based on the screening of different boiled Chinese herbal medicines for inhibiting Edwardsiella tarda, although it is clear that some boiled Chinese herbal medicines have inhibitory effects. Bacteria effect, but its relatively prominent problem is still too high dosage and corresponding use cost when converted into production application, and the value of conversion into production application is low

Method used

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  • A compound plant extract for inhibiting Edwardsiella tarda in water environment and its preparation method and application
  • A compound plant extract for inhibiting Edwardsiella tarda in water environment and its preparation method and application
  • A compound plant extract for inhibiting Edwardsiella tarda in water environment and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] A compound plant extract for inhibiting Edwardsiella tarda in water environments, by weight percentage, weighed 56 parts of the fresh leaf extract of Z. In the environment of 28°C, all the components mentioned above can be mixed, and the obtained mixed liquid is the compound plant extract.

[0071] Wherein, above-mentioned purple back grass fresh leaf extract is obtained by following method:

[0072] (1) Mix the fresh leaves and salt according to the mass percentage (1 / 100) and fully grind them into mash, mix the mash with mature vinegar solution at a mass volume ratio of 1:10, and place at room temperature 33-35°C Airtightly mix in the environment for 8-10 days, keep stirring at intervals of 3-4 hours for 4-5 minutes during the period, and take out the fresh leaves of the vinegar-made purple backgrass;

[0073] (2) Mix the vinegar-made purple-backed grass fresh leaves with water, lactic acid bacteria liquid culture medium (the weight-to-volume ratio is 8:22:5) and lac...

experiment example 1

[0076] Experimental Example 1 Composite Plant Extract of the present invention compares the inhibitory effect of common aquatic pathogenic bacteria

[0077] 1. Test material

[0078] 1.1 Test strains

[0079] Edwardsiella tarda, Aeromonas hydrophila, and Flavobacterium columnar: all are preserved and provided by our research group.

[0080] Povidone-iodine: a 10% povidone-iodine solution.

[0081] 1.2 Test consumables

[0082] TSB medium, Petri dishes, test tubes, distilled water, etc.

[0083] 1.3 Compound plant extracts

[0084] The compound plant extract of the present invention used in this experiment, its preparation method is the same as embodiment 1.

[0085] 2. Test method

[0086] 2.1 Bacterial suspension preparation

[0087] Inoculate Edwardsiella tarda, Aeromonas hydrophila, and Flavobacterium columnar in TSB nutrient broth, culture at 30°C for 16-20 hours, collect the bacteria by centrifugation, and conduct turbidimetric estimation of the bacterial liquid th...

experiment example 2

[0104] Experimental example 2 The compound plant extract prescription compatibility of the present invention is to the inhibition of Edwardsiella tarda

[0105] 1. Test material

[0106] 1.1 Preparation of compound plant extracts

[0107] The composite plant extract of the present invention used in this experiment is named as A, and its preparation method is the same as in Example 1.

[0108] 1.2 Preparation of compound plant extracts with missing prescriptions

[0109]Based on the compound plant extract formula prepared in Example 1 of the present invention, through the technical idea of ​​single-component deletion or double-component simultaneous deletion or three-component simultaneous deletion and the missing components are replaced by PBS in equal amounts to prepare The compound plant extract with single deletion of purple back grass fresh leaf extract, the single deletion compound plant extract of purslane extract, the single deletion compound plant extract of golden b...

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Abstract

The invention belongs to the technical field of antibacterial agents for water environment, and discloses a compound plant extract for Edwardsiella tarda in water environment. The compound plant extract is mainly made of the following components in mass percentage: Extract 55%-65%, Portulaca oleracea extract 26%-35%, Jinbuchang stem extract 12%-18%. Compared with the safety of traditional GMP disinfectants, the compound plant extract of the present invention belongs to environment-friendly substances, and has a large safe concentration range for scaleless fish. When splashed with water, it has obvious inhibitory effect on Edwardsiella tarda, And it will not be disturbed by organic matter in the water body. When the dosage is ≤5mg / L, it can effectively inhibit the abnormal reproduction of Edwardsiella tarda in the upper, middle and bottom layers of the aquaculture water body, and it is effective against the yellow catfish, channel catfish and channel catfish. Security is good.

Description

technical field [0001] The invention relates to the technical field of antibacterial agents for water environment, and more specifically, relates to a compound plant extract for inhibiting Edwardsiella tarda in water environment and its preparation method and application. Background technique [0002] Edwardsiella tarda is a serious Gram-negative pathogen with a size of about 1-4 μm. It is facultatively anaerobic, does not form spores, and uses perinatal flagella as its motility organ. It is commonly found in the world. In freshwater and seawater environments, it can grow at 4-10°C, the optimum growth temperature is 25-32°C, it does not grow above 42°C, and the pH range is 5.5-9. It can grow in the range of 0-4% salt. [0003] Edwardsiella tarda has strong pathogenicity to eel, catfish, flounder, frog and other scale-free cultured species. It enters the fish body by adhering to the bait or invades the fish body through the digestive tract, gills or injured epidermis of the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/22A01P1/00
CPCA01N65/00A01N65/22A01N65/12A01N65/08
Inventor 黄锦炉
Owner 清远海贝生物技术有限公司
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