48 results about "Tachysurus fulvidraco" patented technology

The invention discloses a shrimp, crab and mandarin fish polyculture method, which includes pond construction, clearing and disinfection and shrimp seed stocking; pond construction means that an old pond is altered or a new pond is dug, so that the culture pond can come up to various indexes of standardized fish pond construction; clearing and disinfection mean that after old pond silt clearing, pond embankment leakage prevention and reinforcement, new / old fish pond escape-proof facility arrangement, pond insolation and other works, the whole pond is thoroughly disinfected; the quantity of stocked shrimp seeds is 10 kilograms of freshwater shrimps per mu, and the specification is 1000 to 1400 post-winter shrimp seeds per kilogram; the quantity of stocked silver carp seeds and bighead carp seeds is 30 to 50 per mu; the area of planted waterweeds is about 50 percent of the area of the pond; the quantity of migrated snails is 230 to 260 kilograms per mu; preferably, the quantity of stocked juvenile river crabs is 500 to 800 per mu; preferably, the quantity of stocked loaches is 350 to 450 per mu; and preferably, the quantity of stocked yellow catfishes is 250 to 350 per mu. The method covers the shrimp, crab and mandarin fish polyculture technique and the high-efficiency polyculture technique for a variety of fishes, and forms a unique feasible technical operation route, the polyculture mode is novel, the economic benefit is high, and the ecological environment is good.

The invention discloses an artificial breeding method of tachysurus fulvidraco. The artificial method mainly includes the following steps that firstly, pond conditions are selected, wherein the area of a pond is 8 to 10 mu, the water depth is 1.8 m to 2 m, and quicklime pond cleaning and disinfecting are carried out in the pond before the tachysurus fulvidraco fries are cast; secondly, casting of the tachysurus fulvidraco fries is performed, wherein 6000 to 7000 tachysurus fulvidraco fries per mu are cast and bred in April, feed is cast for feeding from the last ten days of April to November, the conventional feed selected each time is cast according to 1.5 percent to 3 percent of the weight of the tachysurus fulvidraco fries, and the feed is cast twice every day; thirdly, the water quality of the pond is guaranteed; biological agents are used for regulating the water quality once a month from June to September, the amount of the biological agents used each time is 500 g to 1000 g per mu, water is not replaced in the first month and is replaced one to two times after one month, and water which is 10 centimeter to 20 centimeter in depth is replaced each time; fourthly, breeding and oxygenation are performed, wherein one aerator is added to the pond area of every three mu, and the aerators are turned on for oxygenation two hours before each time of feed casting during June to September. According to the artificial breeding method of the tachysurus fulvidraco, the sufficient oxygen content of the pond can be kept, the feed intake amount of the tachysurus fulvidraco fires is increased, in addition, harmful matter in the water can be evaporated while oxygenation is carried out in the pond, and therefore the water quality is adjusted.

The invention relates to a puffed compound feed for yellow catfish and a preparation method thereof. The feed can effectively reduce the use of antibiotics in the breeding process, reduce environmental pollution, and reduce drug residues. The ingredients in a function package can effectively promote the development of immune function and improve the body's own resistance. The perilla extract in the function package can disperse cold, relieve exterior syndrome, and regulate qi by alleviating mental depression. In addition, saffron has a unique fragrance that can be used for food calling; crudeold tea extract contains tea polysaccharides and tea polyphenols, is the best natural medicine to suppress blood sugar elevation, and can stabilize blood sugar in the normal range; and the perilla extract, the crude old tea extract and saffron extract are used in combination to carry out multi-system, multi-path, multi-level and multi-target comprehensive regulation on the yellow catfish bodies, so that the overall disease resistance and the self-healing potential of the yellow catfish bodies are started, and the immunity of the yellow catfish bodies further improved.

The invention discloses a CRISPR / Cas9 system for knocking out the dmrt1 gene at double gRNA sites in yellow catfish. The CRISPR / Cas9 system includes the following steps: (1) Target site 1 is designed on the dmrt1 gene of yellow catfish On the first exon, the target site 2 is designed on the third exon; (2) design primers according to the target site sequence of step (1) to detect the accuracy of the target site in the broodstock, use dmrt1 E1 F and dmrt1 E1 R amplifies target site 1 and nearby sequences, and uses dmrt1 E3 F and dmrt1 E3 R to amplify target site 2 and nearby sequences; (3) Using pUC19‑gRNA‑scaffold plasmid as a template, use dmrt1 E1 gRNA F and gRNA R was used to amplify the gRNA1 fragment by PCR, and dmrt1 E3 gRNA F and gRNA R were used to amplify the gRNA2 fragment by PCR; using the above PCR product as a template, gRNA was obtained by in vitro transcription and purification; (4) the pXT7‑hCas9 linearized plasmid was used as Template, transcribed and synthesized Cas9 mRNA in vitro; (5) Microinjected Cas9 mRNA and two gRNAs into one-cell embryos of yellow catfish; (6) Detected the mutation type and calculated the gene editing rate.

The invention discloses a yellow catfish β-defensin gene and its β-defensin antibacterial peptide and its application; the invention successfully expresses the yellow catfish β-defensin recombinant protein PET‑32a‑Pf_BD using in vitro recombinant expression technology, and inhibits Bacterial activity identification results show that it has a certain antibacterial effect on Staphylococcus aureus in Gram-positive bacteria, Escherichia coli in Gram-negative bacteria, Aeromonas hydrophila, Edwardsiella and Flavobacterium columnar. effect. This laid the foundation for the application of defensin recombinant protein as a broad-spectrum antibacterial drug in aquaculture.

The invention discloses an automatic bait feeding device for aquaculture of yellow catfish. The structure comprises: an upper sealing cover, a storage tank, a main controller, a bottom mounting seat, a feeding hopper, a guiding and feeding body, and a spiral lamp The upper sealing cover is installed on the top of the storage tank and locked with the storage tank. There is a guiding and unloading body under the storage tank. The guiding and unloading body is buckled with the storage tank. The right side of the guiding and unloading body There is a main controller and it is electrically connected with the main controller, so that when the equipment is in use, through the provided mechanism, the present invention can avoid that the baiting equipment can be operated under dark conditions at night due to the daytime and nocturnal characteristics of the yellow catfish. Feeding the feed cannot evenly feed the yellow catfish in the pond in a short period of time. Too much contrast will lead to different body shapes of the same batch of adult fish. Manual night feeding is too troublesome and wastes energy. The equipment saves more time and energy and uses the characteristics of the yellow catfish to feed it more evenly.

The invention relates to a feeding device, in particular to an automatic feeding device for yellow catfish breeding. The invention provides an automatic feeding device for yellow catfish breeding, which is consistent in the amount of the dropped feed each time when the feed is put in, and the feed does not agglomerate. An automatic feeding device for yellow catfish breeding comprises: a traction rope, one end of which is connected with a floating mechanism; and a storage mechanism, which is provided with a storage mechanism on the floating mechanism. Through the floating mechanism and the storage mechanism, the device can float around under the action of the water flow, and the feed leaks at the same time, thereby preventing a large number of yellow catfish from piling up and grabbing for food; the driving mechanism and the stirring mechanism can prevent the feed from being in the storage box. cured.

The invention relates to a method for cultivating Pseudobagrus fulvidraco in a rice field, particularly a stichopus japonicus fertilizer which comprises the following raw materials in parts by weight: 2-4 parts of Bacillus subtilis powder, 1-4 parts of ferment bacteria, 0.5-0.9 part of solid photosynthetic bacteria, 0.8-1.2 parts of bdellovibrio bacteriovorus, 0.2-0.6 part of vitamin additive, 10-16 parts of mineral additive, 0.2-0.4 part of chelator, 60-80 parts of hyacinth, 50-80 parts of edible fungus residue, 100-110 parts of vinasse, 40-60 parts of fishbone dust, 70-80 parts of rice bran, 10-16 parts of brown sugar, 45-55 parts of kongpo monkshood root, 40-50 parts of semen momordicae, 1-15 parts of Stellera chamaejasme and the like. A comparison shows that the composite microbial fertilizer can promote quick growth of stichopus japonicus, increase the EPA content, reduce the fish diseases and the amount of fish dying of illness, and increase the beneficial microbes in the water body, thereby enhancing the final quality and grade.