48 results about "Tachysurus fulvidraco" patented technology

The invention discloses a juvenile pelteobagrus fulvdraco compound feed, and aims at providing the juvenile pelteobagrus fulvdraco compound feed with low feed coefficient, balanced and complete nutrition, strong attractant and good underwater stability. The juvenile pelteobagrus fulvdraco compound feed comprises the following compositions in percentage by weight: 15-25 percent of steam fish meal,5-15 percent of white fish meal, 20-25 percent of flour, 1-5 percent of corn, 20-30 percent of fermented soyabean cake, 5-10 percent of peanut bran, 5-10 percent of sunflower seed cake, 1-10 percent of vegetable cake, 0.5-2 percent of blood cell meal, 2-5 percent of corn protein powder, 0.5-1.5 percent of soya-bean oil, 0.5-1.5 percent of corn oil, 1.5-3 percent of fish oil, 0.8-1 percent of calcium biphosphate, 0.5-1 percent of corncob meal, and the balance of additive containing decavitamin, composite mineral substances, Vc phosphate, choline chloride, salt, mildewproof agents and antioxidant. The feed can be widely applied to the field of cultivating juvenile pelteobagrus fulvdraco.

The invention discloses a feed proportioning method and a cultivation method of juvenile yellow catfish. The proportioning method is characterized in that a juvenile yellow-head catfish feed mainly comprises 9.8-43.2wt% of vegetable protein, 34-8.8wt% of animal protein and the balance of an auxiliary feed. The cultivation method of the yellow-head catfish meat mainly comprises the step of: alternately feeding the juvenile fish by a juvenile fish feed with high-specific gravity animal protein and a juvenile fish feed with low-specific gravity animal protein at time intervals. The conventional feeding mode only uses a feed with single specification in the cultivation of the juvenile yellowcatfish. The invention makes use of the characteristic of the compensation growth of the yellow catfish, and the juvenile fish feed with the high-specific gravity animal protein and the juvenile fish feed with the low-specific gravity animal protein are alternately fed at time intervals, so that the feed efficiency can be improved, and the feed cost can be saved on the premise that the weight increment of the yellow catfish is not influenced.

The invention discloses a shrimp, crab and mandarin fish polyculture method, which includes pond construction, clearing and disinfection and shrimp seed stocking; pond construction means that an old pond is altered or a new pond is dug, so that the culture pond can come up to various indexes of standardized fish pond construction; clearing and disinfection mean that after old pond silt clearing, pond embankment leakage prevention and reinforcement, new / old fish pond escape-proof facility arrangement, pond insolation and other works, the whole pond is thoroughly disinfected; the quantity of stocked shrimp seeds is 10 kilograms of freshwater shrimps per mu, and the specification is 1000 to 1400 post-winter shrimp seeds per kilogram; the quantity of stocked silver carp seeds and bighead carp seeds is 30 to 50 per mu; the area of planted waterweeds is about 50 percent of the area of the pond; the quantity of migrated snails is 230 to 260 kilograms per mu; preferably, the quantity of stocked juvenile river crabs is 500 to 800 per mu; preferably, the quantity of stocked loaches is 350 to 450 per mu; and preferably, the quantity of stocked yellow catfishes is 250 to 350 per mu. The method covers the shrimp, crab and mandarin fish polyculture technique and the high-efficiency polyculture technique for a variety of fishes, and forms a unique feasible technical operation route, the polyculture mode is novel, the economic benefit is high, and the ecological environment is good.

The invention relates to a pseudobagrus fulvidraco puffed formulated feedstuff which is a mixture prepared from materials by using a puffed particle feedstuff processing method. The feedstuff is prepared from raw materials of, by weight: 30-40 parts of fish meal, 20-35 parts of soybean meal, 20-25 parts of flour, 5-10 parts of meat and bone meal, 2-6 parts of maize protein powder, 2-4 parts of soybean oil or fish oil, 1-2 parts of calcium dihydrogen phosphate, 0.8-1 part of potassium salt, 0.8-1 part of sodium salt, 0.8-1 parts of multivitamin, and 0.3-0.8 parts of a plant extract additive. The feedstuff provided by the invention is advantaged in scientific formulation, balanced and comprehensive nutrition, good stability in water, high feeding attraction activity, low feed coefficient, and low pollution to aquaculture water. With the added plant extract additive, the body color of artificially cultivated pseudobagrus fulvidraco can be substantially improved, and the body color of the artificially cultivated pseudobagrus fulvidraco is close to that of wild pseudobagrus fulvidraco. Therefore, the commodity value of the artificially cultivated pseudobagrus fulvidraco can be increased.

The invention discloses a compound feed for a body color restoration period of tachysurus fulvidraco. The compound feed is prepared from the following raw materials in parts by weight: 0.1-0.3 part of microelements, 0.03-0.06 part of vitamins, 1-2 parts of minerals, 20-40 parts of bean pulp, 5-8 parts of corn protein powder, 2-3 parts of alfalfa powder, 15-25 parts of imported fish powder, 0.04-0.08 part of cholate substances, 1-2 parts of spirulina powder, 4-6 parts of kelp powder, 4-6 parts of grease, 0.2-0.4 part of 0.2% lutein and 20-30 parts of flour. The compound feed is balanced in various nutritional ingredients, reasonable in proportion and suitable for quickly growing the tachysurus fulvidraco, and can be used for enabling the body color of thetachysurus fulvidraco to be close to that of wild products.

The invention provides a primer group for identifying a male parent and a female parent of tachysurus fulvidraco and leiocassis longirostris hybrid species. The primer group comprises a primer pair for amplifying COI genes and a primer pair for amplifying an ITS sequence, wherein the primer pair for amplifying COI genes consists of a primer COIF and a primer COIR; the primer pair for amplifying an ITS sequence consists of a primer ITSF and a primer ITSR. The invention also provides a kit which is for identifying the male parent and the female parent of tachysurus fulvidraco and leiocassis longirostris hybrid species and comprises the primer group. The kit also comprises any one or more of the following reagents: a buffer solution for PCR, dNTP, heat-resistant DNA polymerase, a cloning vector, a DNA ligase, and a buffer solution for ligation. The invention also provides a method for identifying the male parent and the female parent of tachysurus fulvidraco and leiocassis longirostris hybrid species. The primer group, the kit or the method can be used for accurately identifying the male parent and the female parent of the tachysurus fulvidraco and leiocassis longirostris hybrid species, thus effectively managing a breeding plan and monitoring negative influence thereof, and has great significance in sustainable development of aquaculture industry.

The invention discloses a breeding method for increasing the survival rate of yellow catfish fry. The method includes the steps of pond selecting, bait breeding, water grass planting, fry putting, bait putting, water quality managing and daily managing. The breeding method is scientific and reasonable; by adopting methods of applying decomposed manure, increasing the oxygen content of a water body in a pond, replacing pond water in time, cleaning a food table, applying sterilize medicine and the like, the water quality is ensured and adjusted, the frequently-seen diseases of yellow catfish are effectively prevented, the immunity of yellow catfish weak in constitution at a fry stage is enhanced, the transition from endogenous nutritive yolk to exogenous nutrients can be well completed for fry, and the fry is balanced in nutrient, high in survival rate and rapid in growth speed and has good breeding and popularizing value.

The invention discloses an exogenous pigment-free special compound feed for tachysurus fulvidraco and a preparation method thereof. The exogenous pigment-free special compound feed for the tachysurus fulvidraco comprises the following components in parts by weight: 18-25 parts of fish meal, 15-20 parts of soybean meal, 10-15 parts of rapeseed meal, 5-10 parts of cottonseed meal, 10-15 parts of corn protein powder, 15-20 parts of flour, 5-10 parts of rice bran, 1-3 parts of monocalcium phosphate, 0.1-0.5 part of multi-vitamin premix, 0.5-1 part of multi-mineral premix, 0.1-0.5 part of choline chloride, 0.2-0.5 part of table salt, 1.5-3.5 parts of soybean oil, 0.01-0.05 part of an antioxidant, 0.1-0.5 part of a mildewproof agent, 2-5 parts of bentonite, 0.1-0.5 part of enveloped lysine, 0.5-1 part of marigold, 0.1-0.5 part of chlorella and 0.1-0.5 part of alfalfa juice protein. The exogenous pigment-free special compound feed for the tachysurus fulvidraco can meet growth and colouring requirements of the tachysurus fulvidraco, growth rate of the tachysurus fulvidraco is increased, feed conversion efficiency is improved, flavour of the tachysurus fulvidraco is enhanced, and meat quality of the tachysurus fulvidraco is improved.

The present invention provides a yellowhead catfish feed which comprises the following components in parts by weight: 70 to 78 parts of agricultural waste poultry and livestock protein substances, 15 to 21 parts of soybean meal, 1 to 3 parts of sesame meal, 3 to 5 parts of gluten flour and 1.5 to 2.5 parts of a YCF additive. The yellowhead catfish feed is produced from pure natural animal and vegetal high-quality raw materials, and has the advantages of easily available materials, wide material sources, appropriate matched nutrition, good viscoelasticity, strong flocculability in water, long floating time, proper fineness, and ingestion easiness for yellowhead catfish. The processing method is simple and convenient, and is low in cost. The yellowhead catfish feed provided by the invention has a strong attractant effect and good palatability, can be rapidly absorbed and easily digested, and contains various amino acids, vitamins and trace elements for the growth and development of yellowhead catfish. In particular, the added YCF additive endues the feed with ideal floating, sinking and drifting states which facilitate the ingestion of yellowhead catfish, and provides good growth promoting and disease preventing effects, so that the immune functions of yellowhead fish are improved, the yield and quality of yellowhead catfish are significantly improved, and the economic benefits are significant.

The invention discloses an artificial breeding method of tachysurus fulvidraco. The artificial method mainly includes the following steps that firstly, pond conditions are selected, wherein the area of a pond is 8 to 10 mu, the water depth is 1.8 m to 2 m, and quicklime pond cleaning and disinfecting are carried out in the pond before the tachysurus fulvidraco fries are cast; secondly, casting of the tachysurus fulvidraco fries is performed, wherein 6000 to 7000 tachysurus fulvidraco fries per mu are cast and bred in April, feed is cast for feeding from the last ten days of April to November, the conventional feed selected each time is cast according to 1.5 percent to 3 percent of the weight of the tachysurus fulvidraco fries, and the feed is cast twice every day; thirdly, the water quality of the pond is guaranteed; biological agents are used for regulating the water quality once a month from June to September, the amount of the biological agents used each time is 500 g to 1000 g per mu, water is not replaced in the first month and is replaced one to two times after one month, and water which is 10 centimeter to 20 centimeter in depth is replaced each time; fourthly, breeding and oxygenation are performed, wherein one aerator is added to the pond area of every three mu, and the aerators are turned on for oxygenation two hours before each time of feed casting during June to September. According to the artificial breeding method of the tachysurus fulvidraco, the sufficient oxygen content of the pond can be kept, the feed intake amount of the tachysurus fulvidraco fires is increased, in addition, harmful matter in the water can be evaporated while oxygenation is carried out in the pond, and therefore the water quality is adjusted.

The invention discloses a descendant determining method for the development of yellow catfishes, snakehead super male fishes and all male fishes. The method comprises the following steps: (1) controlling the sex by the hormone of the yellow catfishes and the snakehead super male fishes; (2) testcrossing and screening pseudo males; (3) obtaining all female groups and pseudo female groups; (4) testcrossing by the pseudo males and screening the pseudo females; (5) testcrossing by the all female groups and screening YY males and YY females to form super male lines. Compared with the prior art, the descendant determining method for the development of the yellow catfishes, the snakehead super male fishes and the all male fishes has the advantages of easiness in operation, high efficiency, low risk in breeding and high testcross efficiency, overcomes the problem of the uncertainty for traditionally developing the all male offspring seeds of XX-XY genotype farmed animals, and reduces the counting difficult.

The invention discloses a cultivation method for cultivating Chinese softshell turtles and yellow-head catfish meats. The method comprises the following steps: (1) draining water in a tank and exposing to sun, sterilizing by uniformly scattering calcined lime for sterilization, applying fermented organic manure after 2-3 days, and equipping an aerator; (2) breeding yellow-head catfish meat fingerlings which are 2-3cm per fish, and Chinese softshell turtle fingerlings which are 0.25-0.5kg per fish, regulating the amount of fodders once every 7-10h, and adding vitamins and / or saccharomycetes which take up to 5-10% of fodders in the fodders every 10-15 days; (4) after 7 days of breeding the fingerlings, spraying trichodina once, filling fresh water every 7-10 day once, and keeping the transparency of the cultivation water to be greater than 30cm; and (5) fishing once every 1-3 months. The healthy cultivation method for cultivating Chinese softshell turtles and yellow-head catfish meats with high efficiency is used for cultivating, so as to reasonably determine the breeding specification and cultivation density, improve the utilization factors of the tank water, control the cultivation risk, improve the comparative benefit of pond culture, therefore, the biological healthful aquaculture of cultivating Chinese softshell turtles and yellow-head catfish meats can be realized.

The invention discloses high-protein feed for promoting the growth of tachysurus fulvidraco fry. The feed is prepared from, by weight, 15-25 parts of corn gluten meal, 6-14 parts of dried-powder of housefly larvae, 3-6 parts of high gluten flour, 2-5 parts of earthworm powder, 4-8 parts of flour weevil powder, 1-3 parts of fish meal, 2-5 parts of bean pulp, 1-4 parts of wheat bran, 3-5 parts of rapeseed meal, 3-6 parts of hericium erinaceus powder, 1-4 parts of chicken intestine powder, 2-5 parts of fish oil, 4-8 parts of bean oil, 1-5 parts of agaric powder, 2-4 parts of shell powder, 1-3 parts of shrimp meal, 3-6 parts of monocalcium phosphate, 1-4 parts of L-ascorbic acid phosphate, 2-5 parts of HJ-1 aquatic adhesive, 1-4 parts of glycine betaine, 2-6 parts of vitamin premix, 3-8 parts of mineral premix, 1-4 parts of choline chloride and 2-6 parts of hydroxymethyl cellulose. The tachysurus fulvidraco fry feed contains rich protein and can promote the growth of tachysurus fulvidraco fry and improve the breeding efficiency and meat quality of tachysurus fulvidraco fry.

The invention relates to a healthy artificial breeding technology of yellow catfishes. The healthy artificial breeding technology comprises the following steps: establishing a breeding pond, cleaning and disinfecting; culturing water; putting young fishes into the breeding pond; domesticating and feeding the young fishes; performing daily management; catching the fishes for marketing. By adopting the healthy artificial breeding technology, the survival rate and growth speed of the yellow catfishes bred artificially can be increased effectively, the yield is increased, the economical benefit is increased, and the development of the yellow catfish breeding industry is promoted.

Provided is a method of alternate culturing of South America white shrimps in a yellow catfish aquaculture pond. The method comprises the steps of 1 offspring seeds purchase on selection and intermediate culture; 2 shrimp seed breeding in an earthen pond; 3 culturing management of the shrimp seeds; 4 the fishing of commercial shrimps for the market; 5 the preparation of the pond and the stocking of yellow catfish seeds; 6 the culturing management of the fish seeds is conducted; 7 the fishing of yellow catfishes. A specific culturing method is adopted in a series of the steps, so that the whole culturing period uses the water body space of the pond reasonably, the fishing carrying capacity is small in high temperature season, the biological prevention and control measure of aquatic product diseases are good, the occurrence rate of disease of aquatic products of scale is low, the efficiency of feed utilization is high, and the economic benefits and ecological benefits of the aquaculture pond are remarkable.

The invention discloses a microsatellite marker relevant with tachysurus fulvidraco growth characteristics and detection and application thereof. That is, four microsatellite loci are found on a tachysurus fulvidraco GH sequence, the microsatellite loci are amplified by screening specific primers, relevance between the microsatellite loci and growth traits is analyzed, and dominant genotypes favorable for the growth traits are found. Results indicate that three loci are remarkably or extremely remarkably relevant with the growth traits, a locus GA is remarkably relevant with body weight, body length, standing height and body thickness and is extremely remarkably relevant with total length and depth of caudal peduncle, wherein CD is a dominant genotype; a locus TCTT is remarkably relevant with head length and depth of caudal peduncle, wherein BD is a dominant genotype; and a locus AC is remarkably relevant with body thickness and head length, wherein DD is a dominant genotype. The four microsatellite loci obtained by primer amplification have high genetic diversity and are remarkably or extremely remarkably relevant with the growth traits. The microsatellite marker is of great significance for molecular breeding practice.

The invention relates to an artificial propagation method of Tachysurus fulvidraco, relates to a freshwater fish cultivation technique and provides a method of laying eggs by ecological induction and propagating Tachysurus fulvidraco by natural hatching. The method comprises the steps of (1) selecting parent fishes; (2) inducing spawning artificially; (3) inducing egg laying ecologically; (4) managing egg laying time and egg laying; (5) hatching; (6) cultivating fry. Tachysurus fulvidraco can be propagated by the method with no need for special hatching facilities, the operating technique is simple, it is very easy for a farmer to master the propagation technique, propagation rate and survival rate are high, propagation cost is low, and it is very easy to popularize and apply the method on large scale. In addition, it is possible to effectively improve the propagating capacity of natural fish resources.

The invention relates to a pond co-culture technology for crayfishes and yellow catfishes. The technology is characterized by co-culturing crayfishes and yellow catfishes, which are a predating variety and a predated variety, in a same pond; the technology is a technology of preventing a floating expanded feed from floating out of a net cage by adhering a plastic film on the net cage. According to a technical scheme adopted by the pond co-culture technology for crayfishes and yellow catfishes, the technology comprises the steps of: I, separated culture of crayfishes and yellow catfishes in the net cage; II, pond condition and preparation; III, structure and placement of the net cage; IV, fry throwing and culture; and V, cultural management. The crayfishes and yellow catfishes, which are a predating variety and a predated variety, are co-cultured in the same pond, so that two high additional value brand-name and high-quality aquatic product varieties which cannot be output by virtue of a conventional technology can be output in one pond. Therefore, the cultural water level is reasonably utilized, and the economical benefit is improved. By adhering the plastic film on the net cage, the floating expanded feed is prevented from floating out of the net cage, so that the cultural cost is saved and lowered.

The present invention relates to a feed for improving body color and meat quality of yellowhead catfish and a preparation method thereof, and belongs to the field of feeds. The feed comprises white fish meal, casein, Antarctic krill powder, corn germ oil, fish oil, cuttlefish liver oil, konjac starch, dextrin, peanut bran, calcium dihydrogen phosphate, compound vitamins, compound minerals, fermented plant powder, a traditional Chinese medicinal additive and porphyridium powder. The feed is reasonable in formula; the components have synergistic and complementary functions; the components are combined to promote the growth of the yellowhead catfish; the feed improves the body color and meat quality of the yellowhead catfish, is good in effects and stable, and can reduce ammonia and nitrogenemission in water. Conditions for producing the green feeds and aquatic products are created. The preparation method is as follows: the raw materials are mixed in ratios. The method is simple and rapid, good in condition controllability, good in feed stability and very good in application prospects.

The invention discloses a river crab and yellow catfish polyculture method. A river crab culture pond with the pond area ranging from 10 mu to 20 mu is selected, the pond depth ranges from 1.2 m to 2.5 m, an oxygenation facility is matched, sterilization is conducted with quicklime of 10-20 kg for each mu 30 days before fry is cast, and fermented and thorough decomposed organic fertilizer of 50-100 kg each mu is cast one week later; the yellow catfish fry is cast around the Tomb-sweeping Day, the specification of the fry ranges from 10 g to 30 g, and 2.5 kg of fry is cast each mu. Due to the fact that the yellow catfish is omnivorous fish, the fry is small in appetite, and pellet feed and small trash fish are cast in the crab pond, the remaining feed of river crabs is enough to feed the yellow catfish, and feed does not need to be additionally cast. After August, along with the growth of the yellow catfish and the increase of the appetite of the yellow cast fish, feed special for the yellow catfish needs to be supplemented, the daily feed cast amount is 20-30 kg each mu, and the feed is cast two times in the morning and at the nightfall. The river crab and yellow catfish polyculture method has the advantages that the culture water body is saved, the culture yield is increased, and the feed is saved.

The invention discloses a method for biannually alternately culturing tilapia mossambica and tachysurus fulvidraco in a pond. By the method, two biannual culture stages can be respectively performed on the tilapia mossambica and the tachysurus fulvidraco in the same pond, and release of large-size tilapia mossambica and tachysurus fulvidraco fry cages for breeding and culturing the tilapia mossambica and the tachysurus fulvidraco are key links of a biannual culture mode. As shown by test results, the method for biannually culturing the tilapia mossambica and the tachysurus fulvidraco has the advantages of high yield and low price as compared with the traditional annular mixed culture mode, and has an obvious economical benefit.

The invention discloses a method of breeding and propagation of yellow-head catfish in Nansi lake. The method of breeding and propagation of yellow-head catfish in Nansi lake includes the steps: selecting inherent wild yellow-head catfish in the lake from different locations in the Nansi lake; after cultivating and establishing a parent fish family, and selecting high quality yellow-head catfish from the parent fish family as the parent fish; after three generations of hybridization, selecting the filial generation with a stable inheritable character, of yellow-head catfish as the parent fish to cultivate in a special pond for breeding the progeny of yellow-head catfish; and after propagation and cultivation, selecting high quality yellow-head catfish among the progeny of yellow-head catfish, releasing the high quality yellow-head catfish in the lake and realizing hybridization between the high quality yellow-head catfish and the wild yellow-head catfish in the lake. Therefore, the method of breeding and propagation of yellow-head catfish in Nansi lake optimizes the character of the wild yellow-head catfish in the Nansi lake, improves the fish production of yellow-head catfish in the lake area, and realizes autonomous breeding and propagation of the high quality yellow-head catfish in the lake.

The invention relates to a puffed compound feed for yellow catfish and a preparation method thereof. The feed can effectively reduce the use of antibiotics in the breeding process, reduce environmental pollution, and reduce drug residues. The ingredients in a function package can effectively promote the development of immune function and improve the body's own resistance. The perilla extract in the function package can disperse cold, relieve exterior syndrome, and regulate qi by alleviating mental depression. In addition, saffron has a unique fragrance that can be used for food calling; crudeold tea extract contains tea polysaccharides and tea polyphenols, is the best natural medicine to suppress blood sugar elevation, and can stabilize blood sugar in the normal range; and the perilla extract, the crude old tea extract and saffron extract are used in combination to carry out multi-system, multi-path, multi-level and multi-target comprehensive regulation on the yellow catfish bodies, so that the overall disease resistance and the self-healing potential of the yellow catfish bodies are started, and the immunity of the yellow catfish bodies further improved.

The invention discloses a hybrid culturing method for bass and crucian by using an ecological-floating-bed-technology-based pond. On the basis of lift habits of various cultured fishes, intercroppingyellow-head catfishes only need to eat residual feeds fully and intercropping crucian plays a role in removing excrements of the bass. When the crucian and yellow-head catfishes, together with the bass, are cultured together in a pond, a phenomenon that the water quality is affected by the residual feed corruption and deterioration is prevented, the ecological balance of the pond is kept, and thecrucian and yellow-head catfish yields are increased additionally. With the biological floating bed technology, water hyacinth is transplanted and the coverage rate of the water hyacinth on the watersurface is controlled, so that nitrogen and phosphorus transformation effect is fully utilized, the dissolved oxygen content in the water is ensured, and biomass of the green alga and blue-green algaeand the like is reduced obviously. Therefore, the purification effect of the culturing pond water is realized; and thus the healthy growth of fishes is promoted effectively and the culturing yield isincreased.

The invention relates to the technical field of freshwater fish aquaculture and specifically relates to fodder for enhancing tachysurus fulvidraco immunity and a preparation method thereof. The fodderis prepared by the following steps: S1, deep frying pork fat, grinding, heating until a material temperature is 130 to 150 DEG C and then adding grapefruit leaf powder to perform thermal insulation reaction to obtain a mixture A for standby application; S2, evenly mixing pig bone meal and chicken bone meal, heating until a material temperature is 180 to 200 DEG C and then adding apple peel to perform thermal insulation reaction to obtain a mixture B for standby application; S3, evenly mixing cassava dregs and bagasse to obtain a mixture C for standby application; S4, taking 1 / 2 of the mixtureC, jointly soaking the mixture C into an xanthan gum solution along with the mixture A and the mixture B, stirring the mixtures in a soaking process, taking suspended matter and the remaining mixtureC to granulate, preparing the mixture into particles with the particle size of 1 to 2 mm and then sequentially spraying microbial powder and hydroxyl modified walnut shell flour. The fodder disclosedby the invention has the advantages of good palatability, easiness in being digested, ability to enhance juvenile fish immunity and ability to reduce ammonium nitrogen content.

The invention discloses a yellow catfish β-defensin gene and its β-defensin antibacterial peptide and its application; the invention successfully expresses the yellow catfish β-defensin recombinant protein PET‑32a‑Pf_BD using in vitro recombinant expression technology, and inhibits Bacterial activity identification results show that it has a certain antibacterial effect on Staphylococcus aureus in Gram-positive bacteria, Escherichia coli in Gram-negative bacteria, Aeromonas hydrophila, Edwardsiella and Flavobacterium columnar. effect. This laid the foundation for the application of defensin recombinant protein as a broad-spectrum antibacterial drug in aquaculture.

The invention discloses an automatic bait feeding device for aquaculture of yellow catfish. The structure comprises: an upper sealing cover, a storage tank, a main controller, a bottom mounting seat, a feeding hopper, a guiding and feeding body, and a spiral lamp The upper sealing cover is installed on the top of the storage tank and locked with the storage tank. There is a guiding and unloading body under the storage tank. The guiding and unloading body is buckled with the storage tank. The right side of the guiding and unloading body There is a main controller and it is electrically connected with the main controller, so that when the equipment is in use, through the provided mechanism, the present invention can avoid that the baiting equipment can be operated under dark conditions at night due to the daytime and nocturnal characteristics of the yellow catfish. Feeding the feed cannot evenly feed the yellow catfish in the pond in a short period of time. Too much contrast will lead to different body shapes of the same batch of adult fish. Manual night feeding is too troublesome and wastes energy. The equipment saves more time and energy and uses the characteristics of the yellow catfish to feed it more evenly.

The invention relates to a method for cultivating Pseudobagrus fulvidraco in a rice field, particularly a stichopus japonicus fertilizer which comprises the following raw materials in parts by weight: 2-4 parts of Bacillus subtilis powder, 1-4 parts of ferment bacteria, 0.5-0.9 part of solid photosynthetic bacteria, 0.8-1.2 parts of bdellovibrio bacteriovorus, 0.2-0.6 part of vitamin additive, 10-16 parts of mineral additive, 0.2-0.4 part of chelator, 60-80 parts of hyacinth, 50-80 parts of edible fungus residue, 100-110 parts of vinasse, 40-60 parts of fishbone dust, 70-80 parts of rice bran, 10-16 parts of brown sugar, 45-55 parts of kongpo monkshood root, 40-50 parts of semen momordicae, 1-15 parts of Stellera chamaejasme and the like. A comparison shows that the composite microbial fertilizer can promote quick growth of stichopus japonicus, increase the EPA content, reduce the fish diseases and the amount of fish dying of illness, and increase the beneficial microbes in the water body, thereby enhancing the final quality and grade.