Microsatellite marker relevant with tachysurus fulvidraco growth characteristics and detection and application thereof

A technology of growth characteristics and microsatellites, applied in the field of molecular marker genetics and breeding

Active Publication Date: 2016-11-30
SOUTH CHINA AGRI UNIV
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  • Application Information

AI Technical Summary

Problems solved by technology

The use of molecular markers to breed high-yield varieties with excellent growth characteristics is one of the important ways to improve economic benefits. Obtaining genes and gene loci related to the growth characteristics of yellow catfish can assist in the breeding of high-yield yellow catfish. Reports on genes and gene loci related to fish growth characteristics

Method used

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  • Microsatellite marker relevant with tachysurus fulvidraco growth characteristics and detection and application thereof
  • Microsatellite marker relevant with tachysurus fulvidraco growth characteristics and detection and application thereof
  • Microsatellite marker relevant with tachysurus fulvidraco growth characteristics and detection and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Screening and typing of microsatellite loci

[0029] The SSRHunter software was used to search the microsatellite loci in the full-length sequence of the GH gene of the yellow catfish to determine the type, location and length of the microsatellite loci. Primer Premier 5.0 software was used to design upstream and downstream primers at about 150 bp upstream and downstream of the SSRs site. See Table 1 for primer information.

[0030]

[0031] In order to realize the fluorescent semi-automatic detection of microsatellite polymorphisms, it is necessary to label the 5' or 3' ends of common primers with fluorescent modification groups. The PCR product amplified by fluorescent primers is detected by electrophoresis on a 96-well capillary 3730xl DNA analyzer. The fluorescent group will emit colored light under the action of the laser. The system automatically collects fluorescent information of different colors, and finally obtains the PCR product. Data informat...

Embodiment 2

[0049] Example 2 SSRs alleles and distribution

[0050] There are 160 experimental samples in this embodiment, 3 alleles are detected at site AT, 4 alleles are detected at site TCTT and site AC, and 5 alleles are detected at site GA. The alleles and distribution of each locus are shown in Table 3.

[0051]

Embodiment 3

[0052] Example 3 Analysis of SSRs genetic growth characteristics

[0053] The genetic characteristics analysis of the 4 SSR polymorphic sites in the study is shown in Table 4. The average heterozygosity of the 4 sites is 0.5661, the sites AT and TCTT are moderately polymorphic, and the sites GA and AC are highly polymorphic polymorphism, indicating that the polymorphism of these 4 microsatellites is high.

[0054]

[0055] In this study, SSRs were typed in 96-capillary ABI3730xl, and the general linear model (GLM) in SAS software was used to compare the genotypes of microsatellite loci and the main growth traits (weight, total length, body length, body height, Body thickness, head length, tail peduncle length, and tail peduncle height) were used for correlation analysis, and the sites with significant differences were tested by variance analysis, and Duncan's method was used for multiple comparisons. The results showed that there was no significant difference in growth tra...

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Abstract

The invention discloses a microsatellite marker relevant with tachysurus fulvidraco growth characteristics and detection and application thereof. That is, four microsatellite loci are found on a tachysurus fulvidraco GH sequence, the microsatellite loci are amplified by screening specific primers, relevance between the microsatellite loci and growth traits is analyzed, and dominant genotypes favorable for the growth traits are found. Results indicate that three loci are remarkably or extremely remarkably relevant with the growth traits, a locus GA is remarkably relevant with body weight, body length, standing height and body thickness and is extremely remarkably relevant with total length and depth of caudal peduncle, wherein CD is a dominant genotype; a locus TCTT is remarkably relevant with head length and depth of caudal peduncle, wherein BD is a dominant genotype; and a locus AC is remarkably relevant with body thickness and head length, wherein DD is a dominant genotype. The four microsatellite loci obtained by primer amplification have high genetic diversity and are remarkably or extremely remarkably relevant with the growth traits. The microsatellite marker is of great significance for molecular breeding practice.

Description

technical field [0001] The invention relates to the technical field of genetic breeding of molecular markers, in particular to a microsatellite marker related to the growth characteristics of yellow catfish and its detection and application. Background technique [0002] Yellow catfish is a small freshwater economic fish widely cultivated in my country. It is popular among consumers because of its tender meat, rich nutrition, and high meat content. The breeding parents of yellow catfish are mainly wild-caught or cultivated populations for several generations. Artificial breeding, its growth rate, disease resistance and breeding performance are weak, so it is of practical significance to carry out two kinds of breeding work of yellow catfish. [0003] At present, many scholars have carried out a large amount of research on the genetic breeding of yellow catfish, and obtained many achievements. With the development of biotechnology, there are more and more methods for fish gen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 刘文生李美娟朱雯璐王娟廖良源
Owner SOUTH CHINA AGRI UNIV
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