Microsatellite marker relevant with tachysurus fulvidraco growth characteristics and detection and application thereof
A technology of growth characteristics and microsatellites, applied in the field of molecular marker genetics and breeding
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0028] Example 1 Screening and typing of microsatellite loci
[0029] The SSRHunter software was used to search the microsatellite loci in the full-length sequence of the GH gene of the yellow catfish to determine the type, location and length of the microsatellite loci. Primer Premier 5.0 software was used to design upstream and downstream primers at about 150 bp upstream and downstream of the SSRs site. See Table 1 for primer information.
[0030]
[0031] In order to realize the fluorescent semi-automatic detection of microsatellite polymorphisms, it is necessary to label the 5' or 3' ends of common primers with fluorescent modification groups. The PCR product amplified by fluorescent primers is detected by electrophoresis on a 96-well capillary 3730xl DNA analyzer. The fluorescent group will emit colored light under the action of the laser. The system automatically collects fluorescent information of different colors, and finally obtains the PCR product. Data informat...
Embodiment 2
[0049] Example 2 SSRs alleles and distribution
[0050] There are 160 experimental samples in this embodiment, 3 alleles are detected at site AT, 4 alleles are detected at site TCTT and site AC, and 5 alleles are detected at site GA. The alleles and distribution of each locus are shown in Table 3.
[0051]
Embodiment 3
[0052] Example 3 Analysis of SSRs genetic growth characteristics
[0053] The genetic characteristics analysis of the 4 SSR polymorphic sites in the study is shown in Table 4. The average heterozygosity of the 4 sites is 0.5661, the sites AT and TCTT are moderately polymorphic, and the sites GA and AC are highly polymorphic polymorphism, indicating that the polymorphism of these 4 microsatellites is high.
[0054]
[0055] In this study, SSRs were typed in 96-capillary ABI3730xl, and the general linear model (GLM) in SAS software was used to compare the genotypes of microsatellite loci and the main growth traits (weight, total length, body length, body height, Body thickness, head length, tail peduncle length, and tail peduncle height) were used for correlation analysis, and the sites with significant differences were tested by variance analysis, and Duncan's method was used for multiple comparisons. The results showed that there was no significant difference in growth tra...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com