58 results about "Bacterial activity" patented technology

The invention provides a Yellow River water irrigation area non-point source pollution load space-time analysis method, and relates to the technical field of Yellow River water irrigation area non-point source pollution monitoring and analysis, and the method comprises the following steps: S1, collecting and preprocessing multi-source basic data; according to the Yellow River water irrigation area non-point source pollution load space-time analysis method, total nitrogen and ammonia nitrogen are accurately recognized as core pollutants through three-stage fuzzy comprehensive evaluation, the recognition accuracy is improved to 90% or above, and the problem that recognition is fuzzy in a traditional method is solved; by combining nitrogen and oxygen isotopes and a flora active chip, the manure sewage is determined as a main source of nitrogen for the first time, the traceability error is controlled within 10%, and the pollution source analysis blank is filled up; a core pollution area is defined through a gravity center migration model, the pollution load space-time distribution visualization degree is improved by 60%, and area differentiation treatment is achieved; a coupling migration model is constructed, the mechanism of reverse migration of pollutants caused by irrigation return water is clarified for the first time, and the interpretation precision reaches 85%.

The invention relates to the technical field of environmental governance and ecological restoration, and discloses a microbial grouting solidification system for mine restoration. Comprising a microorganism propagation and activation module, a cementing liquid preparation and supply module, a double-liquid precise mixing and filling module, a multi-channel distribution and injection module and a real-time monitoring and intelligent control module which are connected with one another, the microbial propagation and activation module comprises a first-stage seed tank, a second-stage propagation tank and an activation storage tank which are sequentially connected through pipelines, and a bacterial liquid activity online detector is arranged at an outlet of the activation storage tank; the cementing liquid preparation and supply module comprises a urea solution storage tank, a calcium salt solution storage tank, a solvent water storage tank and an online dilution and blending unit, and the urea solution storage tank, the calcium salt solution storage tank and the solvent water storage tank are all connected with the online dilution and blending unit through pipelines. According to the system, efficient solidification of a slope rock-soil body, heavy metal fixation and slope stability improvement are achieved, and meanwhile the repair cost is reduced.

The invention relates to the field of intelligent regulation and control, and discloses a factory recirculating aquaculture intelligent control method and system fusing multi-source data. The method comprises the following steps: acquiring environmental chemical index data and microbial activity signal data to obtain a comprehensive real-time monitoring data set; if the dynamic change characteristic of the bacterial community structure indicates that the conversion rate is lower than a conversion threshold value, analyzing the coupling influence of an oxygen supply amount index and a nutrient solution proportion index to obtain an optimized adjustment parameter set; adjusting the balance between the nutrient solution dosage index and the buffer agent dosage index, and determining a final regulation and control scheme; according to the stable output value and the adjustment instruction, fusing the dynamic change characteristics of the bacterial community structure, processing real-time feedback circulation, and determining a system stable state index; and updating the bacterial activity model, judging whether the conversion efficiency reaches a target or not, and obtaining an optimized iteration signal. The problems that an existing system is difficult in interactive analysis, low in regulation and control accuracy, weak in data fusion and unstable in conversion efficiency due to state updating lag are solved.

The invention belongs to the technical field of beverage mixing equipment, and particularly discloses a bifidobacterium beverage for regulating intestinal flora and mixing equipment, the bifidobacterium beverage comprises a bifidobacterium microbial inoculum, prebiotics, a base material and water; the equipment comprises a mixing tank, a rotating frame is rotationally arranged in the mixing tank, rotating blades are rotationally arranged on the rotating frame in the radial direction, a fixing shaft is arranged in the mixing tank, an end face fluted disc is arranged on the fixing shaft, and the ends, facing the center of the fixing shaft, of the rotating blades are meshed with the end face fluted disc; when the rotating frame rotates, the rotating blades synchronously rotate, and the composite motion of the rotating blades can fully cover conical areas at the upper end and the lower end of the mixing tank, so that a material deposition layer is effectively broken, and the problem of non-uniform component distribution caused by mixing dead angles of existing equipment is solved; meanwhile, the motion mode of composite stirring is milder, the local shear force can be obviously reduced, the inactivation of bifidobacterium cells due to mechanical damage is avoided, and the mixing efficiency and bacterial activity protection are both considered.

This invention discloses a culture medium for manganese-oxidizing bacteria suitable for low-carbon source environments in water supply networks and its application. The culture medium is prepared by mixing ferric ammonium citrate, manganese sulfate, ammonium sulfate, sodium chloride, magnesium sulfate, dipotassium hydrogen phosphate, and sodium nitrate, followed by high-temperature and high-pressure sterilization to obtain a culture solution. Manganese sulfate is added to the culture solution to obtain the manganese-oxidizing bacteria culture medium. Manganese-oxidizing bacteria are screened using this culture medium. Cast iron pipe scale is subcultured in the medium to enrich the manganese-oxidizing bacteria culture solution. The enriched manganese-oxidizing bacteria culture solution is diluted and spread onto agar solid medium, and incubated at a constant temperature to obtain manganese-oxidizing bacteria colonies. This invention solves the problem of altered bacterial physiological metabolism caused by high concentrations of added manganese ions, effectively improving bacterial activity and manganese oxidation capacity.

The application discloses a plant Lactobacillus rhamnosus microcapsule and a preparation method and application thereof, and the plant Lactobacillus rhamnosus is embedded by adopting a low-temperature spray drying process with lily starch, citrus pectin and whey protein as wall materials, and mainly includes the following steps: preparation of a bacterial suspension, preparation of a wall material solution, preparation of a microcapsule solution and low-temperature spray drying for preparing the microcapsule. The preparation technology of the stable encapsulated plant Lactobacillus rhamnosus microcapsule provided by the application has the advantages that the viable bacterial count in the microcapsule is high after spray drying, the microcapsule has strong gastrointestinal fluid resistance, is stable in storage, and can effectively solve the problem that the bacterial activity of the plant Lactobacillus rhamnosus and products thereof is reduced or even inactivated in the production and processing, storage and transportation and in-vivo digestion process; the prepared microcapsule can be used as a probiotic bacterial agent and applied to foods such as cheese, yogurt, ice cream, milk, chocolate, lactic acid bacterial beverages and baked products, and helps to promote the healthy development of the probiotic industry, and has obvious economic and social benefits.

This invention provides bfrA Single gene knockout or bfrA Genes and bfrB Double gene knockout of Neisseria gonorrhoeae and its applications involve the field of biotechnology. Research has found that... bfrA Single gene knockout and bfrA and bfrB Double gene knockout of Neisseria gonorrhoeae resulted in increased formation of vesicles on the outer membrane, disruption of the cell wall and cell membrane, reduced adhesion and invasion abilities, decreased bacterial activity, decreased ATP levels, and weakened ability to infect mice. Therefore, knocking out Neisseria gonorrhoeae... bfrA Gene or bfrA and bfrB The dual-gene assay can be used to prepare products for the production of gonococcal outer membrane vesicles and to construct low-pathogenic gonococcal models.

This invention provides a photovoltaic-powered microbial fuel cell constructed wetland system, relating to the field of water pollution control. The system consists of a photovoltaic power supply module, a pulse modulation unit, a mode switching unit, a microbial fuel cell constructed wetland body, an auxiliary anode module, and an electrically isolated stimulation electrode pair. Through photovoltaic power supply and low duty cycle intermittent constant current pulse modulation, it can maintain stable operation for more than 72 hours under no-light conditions. Relying on the time-sharing pulse output of the mode switching unit, electrochemical oxidation of recalcitrant organic matter is achieved through the auxiliary anode. The electroisolated electrode pair stimulates the metabolism of electrogenic bacteria with a non-contact alternating electric field, solving the problems of unstable power supply and difficulty in balancing oxidation efficiency and bacterial activity in off-grid scenarios, thus improving wastewater treatment efficiency and long-term operational stability of the system.

The invention discloses an astaxanthin liposome-coating probiotic compound system based on an interface free radical quenching and bidirectional coupling mechanism and application of the astaxanthin liposome-coating probiotic compound system in oxidative stress protection, and belongs to the technical field of biological medicines and probiotic preparations. According to the preparation method, probiotics with an iron ion mediated sodium alginate-guar gum coating are constructed, and the probiotics and astaxanthin nano-liposomes form an interface protection layer in a hydrogen peroxide stress microenvironment. Wherein astaxanthin protects the key enzyme activity of a thallus energy metabolism pathway, and the thallus continuously consumes a hydrogen peroxide substrate in the environment through an enzyme system of the thallus. According to the composite system disclosed by the invention, under the condition of no chemical coupling, synergistic enhancement of antioxidant defense and thallus activity can be realized through physical blending, the survival rate and colonization ability of probiotics in gastrointestinal stress are remarkably improved, and an innovative solution is provided for targeted intervention of inflammatory bowel diseases and development of functional probiotic preparations.

Composition for the biostimulation of plant growth comprising a bacterial active agent, said bacterial active agent comprising a bacterium of the genus Bacillus and a nitrogen-fixing bacterium, characterized in that the quantity of said bacterium of the genus Bacillus and of said nitrogen-fixing bacterium in said bacterial active agent is greater than or equal to 75% by weight relative to the total weight of said bacterial active agent in the composition.

The invention provides a separating medium, a kit and a method for separating microorganisms in positive blood. The method for separating the microorganisms in the positive blood comprises the following steps: firstly, centrifuging a positive blood culture by adopting a vacuum blood collection tube containing separation gel, and collecting a first separation sample containing the microorganisms to be separated; and then, carrying out centrifugal treatment on the first separation sample by using different separation liquids, and carrying out collection and subsequent identification analysis on the microorganisms in the positive blood according to different densities of the microorganisms to be separated. The traditional culture technology is long in time consumption; in the prior art, microorganisms in a positive blood culture bottle cannot be rapidly and efficiently enriched and purified by pretreatment methods reported in literatures, and especially for gram-positive bacteria, the identification rates of the multiple pretreatment methods combined with mass spectrometry are low, and universality does not exist. The invention provides a rapid purification method which is simple and convenient to operate and high in identification rate and does not influence the bacterial activity, so that the inspection turnover time is shortened, and the clinical diagnosis and treatment efficiency is improved.

This invention discloses a method for preparing κ-carrageenan oligosaccharides and their applications. The preparation method includes the following steps: (1) preparing a mixed solution of κ-carrageenan, H2O2, and ascorbic acid; (2) placing the mixed solution in a reaction environment at a temperature of 40℃-60℃ and a pressure of 20MPa-60MPa for 5min-30min to obtain a κ-carrageenan degradation solution; (3) separating and retaining the κ-carrageenan degradation solution to obtain κ-carrageenan oligosaccharides with a molecular weight of 1000Da-3000Da. The method for preparing κ-carrageenan oligosaccharides provided by this invention uses the H2O2-ascorbic acid system as the main component to oxidize and degrade κ-carrageenan under low temperature and high pressure. It has many advantages such as simple process, high yield, short time consumption, and environmental friendliness, and can be industrialized. The antibacterial composition with the above-mentioned κ-carrageenan oligosaccharides as the active ingredient has good antibacterial activity.

The application relates to a preparation method and application of a Clitopilus passeckerianus strain spore suspension, and the strain has a preservation number of CGMCC No.41407. Through antibacterial activity test on the Clitopilus passeckerianus spore suspension, it is proved that the Clitopilus passeckerianus spore suspension has excellent bacteriostatic activity on plant bacterial diseases, can be applied to preparation of a plant pathogenic bacterial prevention and treatment agent, and has simple preparation process and low production cost.

The invention belongs to the technical field of microorganism preservation, and particularly relates to normal-temperature preservation liquid for group B streptococcus (GBS) in a vaginal swab as well as a preparation method and application of the normal-temperature preservation liquid. The invention provides a GBS normal-temperature preservation solution. The formula of the GBS normal-temperature preservation solution comprises glycerol, glucose, sodium citrate, EDTA, FBS, compound amino acid, NaCl and the like. The preservation solution ensures that GBS can be stably preserved for 30 days at normal temperature, the activity of GBS and the stability of RNA are effectively maintained, the problems of RNA degradation and bacterial activity loss in an existing preservation method are solved, and reliable sample support is provided for subsequent bacterial culture and RNA detection. The method is suitable for long-time preservation and subsequent detection of clinical vaginal swab samples, and has a good application prospect.

The invention relates to composite particles for repairing polluted water and a preparation method and application of the composite particles, and belongs to the technical field of environmental functional materials and water pollution governing. Through double chemical modification of acrylic acid grafting and glutaraldehyde crosslinking, a sodium alginate / chitosan charcoal carrier is modified into a high-strength pH-responsive intelligent carrier, and the composite particles are used for repairing the polluted water. And then the plant growth-promoting bacteria and sodium percarbonate are compounded and granulated. After being added, the carrier can effectively resist SPC initial oxidation impact, protect the activity of thalli, intelligently release PGPB along with the change of pH, accurately link and deepen the oxidative degradation process, and realize efficient and deep mineralization of pollutants. Experiments show that the microbial protection rate of the material is greater than 95%, the 72-hour phenol mineralization rate reaches 85%, and the material is remarkably superior to that of a traditional method. According to the invention, the problem of compatibility between the oxidant and microorganisms is solved, and a new technology is provided for efficient and green water body remediation.

The invention relates to a composition comprising a population of plant-derived extracellular vesicles (EVs) having a diameter ranging from 10 to 500 nm and showing pro-angiogenic, and anti-bacterial activity, for use in therapeutic applications. The invention also relates to a method of loading one or more negatively-charged biologically-active molecules into said population of EVs.

This invention belongs to the field of fermented dairy product technology. It discloses a mildly acidic jujube seed fermented milk containing sleep-regulating functional components, its preparation method, and its applications. A mildly acidic fermenting agent is used, employing a compound of *Streptococcus thermophilus* subsp. *salivarius* to replace *Lactobacillus bulgaricus* in traditional fermenting agents, thus avoiding post-acidification problems at the source of the bacterial strain. The process of adding ultrafine jujube seed powder eliminates the inhibition of bacterial activity and off-flavors caused by co-fermentation, while also inhibiting post-acidification of the product. The jujube seed fermented milk containing sleep-regulating functional components prepared using the above method has a stable GABA content of over 64 mg / 100g, and its pH remains above 4.0 after 21 days of storage at 15℃. This invention is simple to operate, has a short fermentation cycle, significantly improves flavor appeal, and is suitable for industrial production and the preparation of various fermented dairy products.

EnzyFloss is an innovative interdental cleaning device that integrates diagnostic and oral health functionality into a standard dental flosser. The floss string is coated with a unique combination of enzymes: amylase, protease, and lipase, long with a pH-sensitive dye and candelilla wax, enabling the detection of early oral health issues such as plaque accumulation, bacterial activity, or imbalanced oral pH. The biodegradable handle is ergonomically designed and inscribed with inspirational scripture for user encouragement. This multifunctional design advances the art by transforming flossing into a proactive oral health screening tool, unlike traditional flossers which only provide mechanical cleaning.

The utility model provides a garbage penetrating fluid recovery processing device which comprises a waste liquid processing tank, a bacteria liquid storage cylinder, an electromagnetic valve, a mixing cylinder, a discharge pipeline, a suction pipeline and a hydraulic telescopic rod, the bacteria liquid storage cylinder is arranged in the waste liquid processing tank, the electromagnetic valve is installed in the bacteria liquid storage cylinder, the mixing cylinder is arranged in the waste liquid processing tank, and the discharge pipeline is communicated with the suction pipeline. A discharge pipeline is arranged on the outer side face of the mixing cylinder, a suction pipeline is arranged on the outer side face of the mixing cylinder, a hydraulic telescopic rod is arranged in the mixing cylinder, and the problems that the activity of bacteria in bacterial liquid is affected and the treatment efficiency is reduced due to stirring and mixing of an original garbage penetrating fluid recycling treatment device are solved through the design. The bacteria liquid and the garbage penetrating fluid are mixed in a suction and pump-out mode, the activity of bacteria in the bacteria liquid is ensured, meanwhile, the bacteria liquid and the garbage penetrating fluid are more rapidly and evenly mixed so as to facilitate reaction, use is easy and convenient, and practicability is high.

The application discloses a 1,3,4-oxadiazole sulfide compound containing an isopropanolamine structure and a preparation method and application thereof. The preparation method of the 1,3,4-oxadiazole sulfide compound containing the isopropanolamine structure is simple in operation, does not involve a noble metal in the reaction, and is efficient. The 1,3,4-oxadiazole sulfide compound containing the isopropanolamine structure has good bactericidal activity, and the 1,3,4-oxadiazole sulfide compound containing the isopropanolamine structure shows efficient and / or broad-spectrum bacteriostatic activity, and can be effectively used in the prevention and treatment of crop pathogenic bacteria.

The invention provides a constant-temperature freezer integrating soft freezing and high-voltage electric field unfreezing functions, which comprises a freezer body, a soft freezing system, a high-voltage electric field generator, a control system and a power supply module, and the power supply module supplies power to the soft freezing system, the high-voltage electric field generator and the control system respectively. According to the soft refrigeration system, the design that the cold storage agent is injected into the cold storage agent storage plate is innovatively adopted, and an efficient and stable refrigeration constant-temperature space is constructed; the high-voltage electric field generator generates a high-voltage electric field, so that the temperature control precision of the refrigeration equipment can be optimized, the temperature of a refrigeration / freezing space is more stable and better meets the antibacterial requirement, and meanwhile, the polarization effect of the electric field on bacterial cell membranes is utilized to destroy the metabolism and reproduction structures of the bacterial cell membranes and assist in reducing the bacterial activity.

The invention relates to the technical field of medical equipment. The invention discloses a cross infection prevention type human body excrement drainage and collection device, and aims to solve the problems that the bacterial activity of excrement is relatively high in a normal temperature state, cross infection is easily generated in the excrement conveying or transferring process, and solid excrement inhibiting the bacterial activity after freezing is difficult to freeze in the freezing process. The excrement formed into ice blocks is easy to adhere to the inner wall of a container for containing the excrement, the excrement and the container are difficult to separate, and the manual operation process is not sanitary. According to the device, the problem of cross infection easily caused by bacteria breeding of excreta is avoided in a quick freezing mode, meanwhile, the problem that frozen excreta is easily adhered in a freezing forming container and is not easy to separate is solved, and collection, freezing forming, discharging, storage and conveying of the excreta can be achieved only through one driving component; the equipment cost is saved; meanwhile, the excrement treatment efficiency is also improved.

The present application relates to a quality control product for quality evaluation of a preparation for intestinal flora transplantation, a preparation method and application thereof. The quality control product comprises a complex microbial component, an internal standard component, a simulated matrix and a stabilizer. The present application adopts a "multi-donor mixing" method, and compared with a scheme based on cultured strains, the quality control product prepared by the present application is highly consistent with a real FMT preparation in terms of species composition, relative proportion and bacterial activity state, and the quality control result has a strong clinical relevance. By constructing a "core bacterial spectrum", the complex flora quality control becomes simple, focused and cost controllable. In addition, the use of bifidobacterium as an internal standard has better compatibility with the sample matrix, more accurate monitoring and completely eliminates the risk of exogenous contamination.

The present application relates to a composition for cryopreservation of microalgae belonging to the Thraustochytridaceae family, and a method for cryopreservation of microalgae belonging to the Thraustochytridaceae family using the same. According to one aspect, by the composition for cryopreservation of microalgae belonging to the Thraustochytridaceae family and the method for cryopreservation of microalgae belonging to the Thraustochytridaceae family using the same, the microalgae can be stably stored for a long time, and the cost of preserving the microalgae can be reduced by shortening the process. Furthermore, according to the method of preparing a freeze-dried biomass of microalgae belonging to the Thraustochytridaceae family using the same, even during long-term storage at room temperature, a freeze-dried biomass in the form of a powder capable of maintaining bacterial activity and easy to store and transport can be manufactured through a simple process.

The application provides a silk fibroin-gelatin hydrogel material embedding bacterial strains and a preparation method and application thereof, and belongs to the technical field of microorganism embedding. After gelatin is crosslinked with silk fibroin, a "double network" hydrogel can be formed, wherein the beta-fold domain of the silk fibroin provides a rigid skeleton, the spiral chain of the gelatin provides elasticity and cell binding sites, and both have high mechanical toughness and excellent cell affinity, and are ideal carriers for embedding probiotic bacteria or therapeutic bacterial strains. The application adopts a composite hydrogel embedding system taking silk fibroin-gelatin as a matrix, and after bacterial strains are embedded, the activity, stress resistance and functional expression of the bacterial strains can be significantly improved, and the problems of poor biocompatibility and rapid decline of bacterial activity of traditional embedding materials are solved.

The invention belongs to the technical field of microorganisms, and particularly relates to a composite microbial preparation and a preparation method thereof.The composite microbial preparation comprises an inner core, a middle layer and an outer layer; the inner core is an agricultural microorganism, the middle layer is a metal phenolic network layer, and the outer layer is a wrapping layer; the agricultural microorganisms comprise bacillus cereus and / or bacillus pumilus; the viable concentration of the agricultural microorganisms is greater than or equal to 1 * 10 < 12 > CFU / mL; and the metal phenolic network layer is prepared from the following raw materials: polyphenol and ferric salt. The compound microbial preparation is stable in property, has good slow release and degradation effects, can be preserved for a long time, does not need a cold chain in the storage or transportation process, and is convenient to carry and transport.

This invention provides a fecal microbial freeze-drying protective solution. Through the synergistic effect of components such as phosphorylcholine polymer, glycerol, trehalose, ergothioneine, L-cysteine, melibiose, isomaltooligosaccharide, and stachydrine, it effectively reduces the damage to bacterial activity during the freeze-drying process, improves the stability of freeze-drying preservation, and enhances the ability to maintain bacterial metabolic activity. It plays a significant role in maintaining the diversity and functional activity of the bacterial community.

The invention relates to the technical field of medicine, in particular to a pyrrole quinazoline diamine derivative and a preparation method and application thereof, the preparation method of the pyrrole quinazoline diamine derivative is mild in reaction condition, the reaction process is easy to operate, and used reagents are low in price and easy to obtain. The compound has excellent broad-spectrum antibacterial activity, bactericidal ability and drug resistance, and has good biological safety to cells. Cell membrane permeability experiments and transmission electron microscopes show that the compound can destroy bacterial cell membranes, so that intracellular substances leak to cause bacterial death; a dihydrofolate reductase inhibition experiment shows that the compound has high affinity with e-DHFR and can effectively inhibit enzyme activity. The pyrroloquinazoline diamine derivative compound provided by the invention effectively inhibits the growth of bacteria and resists the generation of drug resistance through the double-target action of bacterial DHFR activity inhibition and bacterial cell membrane destruction.

The product for reduction of body odours disclosed herein contains 60 to 98.5% of demineralized water by weight, 0.2 to 5% of cellulose gum by weight, 0.5 to 10% of salt by weight, 0.2 to 5% of preservative by weight, 0.5 to 5% of capsules containing the neutralizer for unpleasant odours by weight, 0.1 to 5% of disinfectant by weight, 0 to 5% of tenside by weight and 0 to 5% of fragrance by weight. The product itself is applied directly to the fabric (e.g. T-shirt) or sports equipment (e.g. sports shoes) and is left to dry. During the drying process, a thin layer of nanoparticles containing an encapsulated neutralizer for unpleasant odours is created. When such items that have been treated with this product are worn, the cracking of nanocapsules occurs due to friction and a discrete fragrance that neutralizes the body odour (bacterial activity) is released.

The invention belongs to the technical field of image processing, and particularly relates to a desulfurization strain activity parameter intelligent detection method and system based on a hyperspectral image, and the method comprises the steps: extracting the original reflectivity of each pixel point in a desulfurization strain liquid hyperspectral image under a continuous wave band; anchoring a local background baseline by using the original reflectivity at the first and second reference wavebands, and determining the metabolic absorption depth by combining the original reflectivity at the characteristic metabolic waveband; determining a spatial spectrum gradient offset based on the original reflectivity difference between each pixel point and the spatial neighborhood pixel point thereof at the structure scattering wave band and the metabolic absorption depth difference between the pixel points; and determining strain activity evaluation parameters according to the spatial spectrum gradient offsets and the metabolic absorption depths of all the pixel points. According to the method, interference of background water fluctuation and high-density inactivated bacterial liquid can be eliminated, real characterization of the microbial metabolism intensity is realized, and the monitoring accuracy and stability of a desulfurization system are improved.