149 results about "Bacterial activity" patented technology

The invention provides a sewage treatment detection method and system with a diagnosis function, and the method comprises the steps: firstly receiving multi-source detection data which is uploaded by each treatment unit in a sewage treatment process and comprises sewage pollutant component data, treatment equipment operation parameter data and flora activity data in a reaction tank; performing cross-unit association mapping processing on the multi-source detection data to generate a substance transformation association network among processing units, constructing a dynamic balance model of the sewage treatment system based on the network, inputting the multi-source detection data acquired in real time into the model, analyzing abnormal association nodes deviating from a balance state and corresponding abnormal influence factors, and determining the abnormal association nodes and the corresponding abnormal influence factors; and finally, generating a diagnosis report containing an abnormal processing path and an equipment regulation and control instruction according to an analysis result, thereby accurately positioning a problem source of the sewage treatment system, timely adjusting equipment parameters to recover system balance, and improving the processing efficiency and stability.

The invention discloses a leak-free core-shell hydrogel microsphere delivery system for non-contact flora transplantation as well as a construction method and application of the leak-free core-shell hydrogel microsphere delivery system. According to the invention, bacteria are encapsulated in a core of a core-shell microsphere through a two-step photo-initiation emulsion polymerization method of methacrylic acid gelatin, and a non-contact microbiota transplantation system (NMTS) is constructed. The NMTS can be used for biological encapsulation of various strains, shows encapsulation efficiency close to 100%, and has little influence on bacterial activity at the same time. NMTS demonstrates: a shielding effect that protects sealed bacteria from low pH values in the gastrointestinal tract and bile acid disruption; sufficient mechanical strength ensures that bacterial leakage is prevented and the infection risk is eliminated in the whole gastrointestinal tract delivery process; and internal and external substance exchange is realized, and the intestinal microorganism structure is remodeled. The invention provides a unique bacterial cell microencapsulation method which is a non-contact, safe and effective micropopulation transplantation method.

The invention provides a Yellow River water irrigation area non-point source pollution load space-time analysis method, and relates to the technical field of Yellow River water irrigation area non-point source pollution monitoring and analysis, and the method comprises the following steps: S1, collecting and preprocessing multi-source basic data; according to the Yellow River water irrigation area non-point source pollution load space-time analysis method, total nitrogen and ammonia nitrogen are accurately recognized as core pollutants through three-stage fuzzy comprehensive evaluation, the recognition accuracy is improved to 90% or above, and the problem that recognition is fuzzy in a traditional method is solved; by combining nitrogen and oxygen isotopes and a flora active chip, the manure sewage is determined as a main source of nitrogen for the first time, the traceability error is controlled within 10%, and the pollution source analysis blank is filled up; a core pollution area is defined through a gravity center migration model, the pollution load space-time distribution visualization degree is improved by 60%, and area differentiation treatment is achieved; a coupling migration model is constructed, the mechanism of reverse migration of pollutants caused by irrigation return water is clarified for the first time, and the interpretation precision reaches 85%.

The invention discloses a far-infrared functional photobiotin composite material, an acupoint patch prepared from the far-infrared functional photobiotin composite material and application of the acupoint patch, and belongs to the technical field of far-infrared medical materials. The photobiotin composite material is prepared from the following components in parts by weight: 25 to 45 parts of modified stone needle, 20 to 30 parts of medical stone, 10 to 20 parts of germanite, 5 to 10 parts of silicon dioxide and 5 to 10 parts of aluminum oxide. The modified stone needle is prepared by processes of bentonite interlayer chambering, LaFe perovskite / TiO2 quantum dot intercalation, Prussian blue analogue loading and the like. The composite material has a far infrared emission characteristic and can generate resonance absorption with human tissues, negative oxygen ions are released through quantum dot photocatalysis, and a porous structure adsorbs inflammatory factors, namely LaFe perovskite / TiO2 quantum dot photocatalysis and cooperates with stone needle far infrared radiation to destroy bacterial cell membranes; and the medical stone releases trace elements to inhibit bacterial activity. The prepared acupoint patch is compounded with a medical pressure-sensitive adhesive through a compression molding process to generate micro-current to stimulate a TRPV1 ion channel, so that the treatment of facial paralysis, hypertension and diabetes mellitus is realized.

The invention belongs to the field of microorganisms, and particularly relates to a banana endophyte compound fungicide as well as a preparation method and application thereof. According to the invention, a novel endophytic bacillus velezensis JDB15 strain and an endophytic trichoderma harzianum JDL4 strain are separated from a banana plant. The trichoderma harzianum JDL4 can inhibit botrytis cinerea, fusarium graminearum, rhizoctonia solani, peach anthracnose pathogen, peach brown rot pathogen and alternaria alternata, and has a good broad-spectrum antibacterial effect. Meanwhile, after the filtrate of the fermentation liquor of the trichoderma harzianum JDL4 and the bacillus velezensis JDB15 are compounded, the antibacterial effect is remarkably improved, and the control effect on banana fusarium wilt, pyricularia oryzae, tomato fusarium wilt and corn bipolaris maydis is also remarkably improved. The compound bacterial agent has wide biocontrol bacterial activity, can be developed deeply subsequently, and provides a sustainable and green solution for prevention and treatment of banana wilt and other plant diseases.

The invention provides a device and a method for short-cut nitrification / anaerobic ammonia oxidation high-rate denitrification of low-ammonia-nitrogen wastewater in a tropical region, belongs to the technical field of biological sewage treatment, and is used for treating the low-ammonia-nitrogen wastewater in the tropical region. The device comprises a water inlet tank, a double-zone bioreactor and a water collecting tank. The method comprises the following steps: pre-treating floc sludge in a reactor by adding hydroxylamine and controlling sludge age, so as to keep the activity of ammonia oxidizing bacteria and inhibit the activity of nitrite oxidizing bacteria at the same time; after pretreatment, anaerobic ammonia oxidation granular sludge is inoculated into the reactor, and aeration is performed at the upper part of the reactor to provide an aerobic environment for ammonia oxidizing bacteria in floc sludge; the granular sludge is settled at the lower part of the reactor, and nitrite generated by floc sludge at the upper part flows back to the lower part, so that anaerobic ammonia oxidation denitrification is realized. The two functional microorganisms are placed in respective appropriate environments, so that the growth of nitrite oxidizing bacteria can be controlled, the short-cut nitrification / anaerobic ammonia oxidation rate is increased, and the high-rate denitrification of the low-ammonia-nitrogen wastewater is realized.

The invention discloses a method and a system for optimally proportioning a microbial fertilizer protective agent and a preservative. The method is applied to a system comprising a cloud computing server, an edge computing node and a laboratory detection unit, environmental parameters such as temperature, humidity and pH value and bacterial activity data of the microbial fertilizer are collected through the edge computing node, and the data are uploaded to the cloud computing server. And the cloud computing server constructs a formula optimization model based on the collected data, calculates and outputs an optimal proportioning scheme, and controls the edge computing node to monitor and verify the bacterium activity stability of the optimal proportioning scheme. And dynamically adjusting the proportioning parameter of the protective agent and the preservative according to the verification result. The system integrates a high-precision sensor, data acquisition equipment and laboratory detection equipment, so that intelligent optimization of the ratio of the microbial fertilizer protective agent to the preservative is realized. The bacterial activity stability of the microbial fertilizer can be effectively improved, and the storage life is prolonged.

The invention provides a preparation method of an anti-ultraviolet antibacterial multifunctional polyester fiber, and belongs to the technical field of melt spinning. White polyester chips are fed into a mixing bin through a main bin, a first functional chip is fed into the mixing bin through a first distribution bin, a second functional chip is fed into a small screw through a second distribution bin, the first functional chip is nano TiO2, the second functional chip is a nano silver antibacterial agent, melt extrusion and spinning are performed, the breaking strength of the obtained uvioresistant and antibacterial functional polyester fiber is 84.4 N, the breaking strength is 7.6 cN / dtex, and the uvioresistant and antibacterial functional polyester fiber is obtained. The dry heat shrinkage rate is 6.0%, and the elongation at break is 15.9%; the anti-ultraviolet performance is high, and the breaking strength retention rate is 90% or above; the bacterial activity value is less than-1.0, and no fungus is bred. A signal feedback device for detecting the aging strength retention rate and the silver ion content is arranged before the packaging process, and the adding speed and the adding amount of the first function slice and the second function slice are controlled.

The invention relates to the technical field of home textile fabric preparation, in particular to an antibacterial and anti-mite home textile fabric and a preparation process thereof. The preparation process comprises the following steps: preparing a zinc zeolite carboxylic acid composite material; purified nano platinum is prepared; and preparing the antibacterial and anti-mite home textile fabric. Zinc and methylimidazole are compounded to form a zinc zeolite structure similar to zeolite, 1, 3, 5-benzenetricarboxylic acid is added into the zinc zeolite structure to form a composite metal organic framework, and the composite metal organic framework can enlarge the contact area with bacteria, so that 1, 3, 5-benzenetricarboxylic acid has an opportunity to contact with key metabolic enzymes in bacterial cells, and the bacterial activity is improved. Meanwhile, the zinc ions can be slowly released along with time after the composite metal organic framework is combined with the zinc ions, and the slowly released zinc ions have the antibacterial characteristic and can interfere the metabolic process of bacteria and inhibit the growth of the bacteria, so that the long-acting antibacterial effect is achieved.

The invention relates to the technical field of environmental governance and ecological restoration, and discloses a microbial grouting solidification system for mine restoration. Comprising a microorganism propagation and activation module, a cementing liquid preparation and supply module, a double-liquid precise mixing and filling module, a multi-channel distribution and injection module and a real-time monitoring and intelligent control module which are connected with one another, the microbial propagation and activation module comprises a first-stage seed tank, a second-stage propagation tank and an activation storage tank which are sequentially connected through pipelines, and a bacterial liquid activity online detector is arranged at an outlet of the activation storage tank; the cementing liquid preparation and supply module comprises a urea solution storage tank, a calcium salt solution storage tank, a solvent water storage tank and an online dilution and blending unit, and the urea solution storage tank, the calcium salt solution storage tank and the solvent water storage tank are all connected with the online dilution and blending unit through pipelines. According to the system, efficient solidification of a slope rock-soil body, heavy metal fixation and slope stability improvement are achieved, and meanwhile the repair cost is reduced.

The invention relates to a high-performance antibacterial PP plate and a preparation method thereof, and belongs to the technical field of high polymer materials. The high-performance antibacterial PP board comprises the following components in parts by weight: 60-80 parts of PP resin, 1-4 parts of a dispersing agent, 0.5-1.5 parts of a modified antibacterial agent, 2-6 parts of a modified flame retardant and 0.1-0.5 part of an antioxidant, nano-zinc oxide in the modified antibacterial agent is excited to have strong oxidizing property under ultraviolet light, destroys cell membranes, releases zinc ions to be combined with proteins and interferes bacterial enzyme activity, quaternary ammonium salt groups destroy cell membrane structures, and the quaternary ammonium salt groups and chitosan cooperate to destroy cell wall structures and permeate into the interior to inhibit bacterial activity; boron-based components in the modified flame retardant are heated to dehydrate, carbon formation is accelerated, a glassy protective layer is formed, a Si-O-B structure is constructed, incombustible gas is released, heat and oxygen are insulated, and the modified flame retardant and phosphorus-oxygen double bonds are synergistically flame-retardant; the high-performance antibacterial PP board prepared by the invention not only has a good antibacterial effect, but also has an excellent flame-retardant effect.

The invention relates to the field of intelligent regulation and control, and discloses a factory recirculating aquaculture intelligent control method and system fusing multi-source data. The method comprises the following steps: acquiring environmental chemical index data and microbial activity signal data to obtain a comprehensive real-time monitoring data set; if the dynamic change characteristic of the bacterial community structure indicates that the conversion rate is lower than a conversion threshold value, analyzing the coupling influence of an oxygen supply amount index and a nutrient solution proportion index to obtain an optimized adjustment parameter set; adjusting the balance between the nutrient solution dosage index and the buffer agent dosage index, and determining a final regulation and control scheme; according to the stable output value and the adjustment instruction, fusing the dynamic change characteristics of the bacterial community structure, processing real-time feedback circulation, and determining a system stable state index; and updating the bacterial activity model, judging whether the conversion efficiency reaches a target or not, and obtaining an optimized iteration signal. The problems that an existing system is difficult in interactive analysis, low in regulation and control accuracy, weak in data fusion and unstable in conversion efficiency due to state updating lag are solved.

The invention discloses a biocontrol Chryseobacterium sp. With'contact sterilization 'activity. The PcE1 is a biocontrol bacterium which is separated from rhizosphere soil of Chinese cabbage by utilizing a germ enrichment method and can inhibit the growth of various pathogenic bacteria in a cell contact manner. The genome of the PcE1 encodes a type VI secretion system (T6SS) gene cluster and a plurality of T6SS effect proteins with antibacterial activity. The biocontrol effect determination on plants shows that the PcE1 can inhibit the infection of pectobacterium carotovorum on carrots and also can inhibit the infection of citrus canker pathogens on citrus.

The present invention discloses a far-infrared functional bioluminescent composite material, an acupuncture patch prepared from the composite material, and its application, belonging to the technical field of far-infrared medical materials. The bioluminescent composite material is composed of the following components by weight: 25-45 parts of modified Bianstone, 20-30 parts of medical stone, 10-20 parts of germanium stone, 5-10 parts of silicon dioxide, and 5-10 parts of aluminum oxide. The modified Bianstone is prepared by processes such as bentonite interlayer pore expansion, LaFe perovskite / TiO2 quantum dot intercalation, and Prussian blue analog loading. The composite material has far-infrared emission characteristics and can resonate with human tissue to absorb negative oxygen ions through quantum dot photocatalysis. The porous structure adsorbs inflammatory factors. The LaFe perovskite / TiO2 quantum dot photocatalysis synergizes with the far-infrared radiation of the Bianstone to destroy bacterial cell membranes; the medical stone releases trace elements to inhibit bacterial activity. The prepared acupuncture patch is compounded with a medical pressure-sensitive adhesive through a molding process to generate microcurrent to stimulate the TRPV1 ion channel, achieving the treatment of facial paralysis, hypertension, and diabetes.

The invention belongs to the technical field of beverage mixing equipment, and particularly discloses a bifidobacterium beverage for regulating intestinal flora and mixing equipment, the bifidobacterium beverage comprises a bifidobacterium microbial inoculum, prebiotics, a base material and water; the equipment comprises a mixing tank, a rotating frame is rotationally arranged in the mixing tank, rotating blades are rotationally arranged on the rotating frame in the radial direction, a fixing shaft is arranged in the mixing tank, an end face fluted disc is arranged on the fixing shaft, and the ends, facing the center of the fixing shaft, of the rotating blades are meshed with the end face fluted disc; when the rotating frame rotates, the rotating blades synchronously rotate, and the composite motion of the rotating blades can fully cover conical areas at the upper end and the lower end of the mixing tank, so that a material deposition layer is effectively broken, and the problem of non-uniform component distribution caused by mixing dead angles of existing equipment is solved; meanwhile, the motion mode of composite stirring is milder, the local shear force can be obviously reduced, the inactivation of bifidobacterium cells due to mechanical damage is avoided, and the mixing efficiency and bacterial activity protection are both considered.

The present invention relates to methods of activating live lactic acid bacteria comprising exposing said bacteria to a preparation comprising citrate, wherein said bacteria have the ability to utilize citrate as an external electron acceptor. The present invention further relates to methods to enhance the activity of certain live bacteria in mammals. More specifically the invention relates to improve the wake-up of certain lactic acid bacteria from the freeze-dried state. The present invention also relates to preparations comprising said activated bacteria and therapeutic uses of said activated bacteria.

The invention belongs to the technical field of microorganisms, and particularly relates to lactobacillus plantarum HNGD-YC1 capable of degrading free-state advanced glycosylation end products (AGEs) and application of the lactobacillus plantarum HNGD-YC1. The strain is classified and named as Lactobacillus plantarum, and the preservation number of the strain is CCTCC M 20251498. The strain can effectively degrade free AGEs, and has good acid resistance and cholate resistance. Besides, lactic acid bacteria are adopted for degrading free AGEs in food for the first time, the degradation rate is 69.3%, and the lactobacillus has an excellent probiotic function, is high in strain activity, can easily pass through the stomach and be planted in the intestinal tract and is low in economic cost; the invention provides a novel safe and sustainable method for solving the problem that the food-sourced harmful factor AGEs enters the body to damage the health, and also provides a safer and more effective diet strategy for people with AGEs-related metabolic diseases, thereby being beneficial to promoting the healthy life of people.

The invention relates to the technical field of pesticide compounding, and discloses a bactericidal compound composition containing quinolone compounds and application thereof. The composition comprises effective active components, the effective active components comprise a compound I and a compound II, the compound I is 7-chloro-1-cyclopropyl-6-fluoro-4-oxo-1, 4-dihydroquinoline-3-carboxylic acid-2-methoxyethyl ester, and the compound II is other compounds with bacterial killing activity, and the compound I and the compound II are selected from the group consisting of 7-chloro-1-cyclopropyl-6-fluoro-4-oxo-1, 4-dihydroquinoline-3-carboxylic acid-2-methoxyethyl ester and 7-chloro-1-cyclopropyl-6-fluoro-4-oxo-1, 4-dihydroquinoline-3-carboxylic acid-2-methoxyethyl ester. The bactericidal compound composition disclosed by the invention has the following advantages: (1) the bactericidal compound composition has a synergistic effect and can improve the prevention and treatment effect; (2) the bactericidal spectrum is expanded, field diseases are mixed, and the effect on bacterial diseases is stronger; (3) the two effective components are mixed for use, so that the drug resistance of pathogenic bacteria can be delayed; and (4) the application amount is reduced, the use frequency is reduced, and the use cost is reduced.

The invention discloses a 1, 8-disubstituted tryptanthrin derivative and application of the 1, 8-disubstituted tryptanthrin derivative to prevention and treatment of phytopathogen and anti-tumor activity, the general formula of the derivative is shown in (I), and the test result of the compound shown in the general formula on the bacterial activity of phytopathogen shows that the compound has a good antibacterial effect on Xanthomonas oryzae pv. Oryzae, Xanthomonas citri pv. Citri and the like, and can be used for preventing and treating the phytopathogen. The method can be used for developing novel agricultural bactericides. Meanwhile, results of the compound shown in the general formula on tumor cells show that the compound has good in-vitro anti-tumor activity on human chronic myelogenous leukemia cells K562, human lung cancer cell lines A549, human liver cancer cells HepG2, human prostate cancer cells PC3 and the like, and can be used for researching novel anti-tumor drugs. # imgabs0 #

The invention belongs to the technical field of fermentation engineering, and particularly relates to lactobacillus reuteri with high beta-glucosidase activity and application of the lactobacillus reuteri in fermentation of sea-buckthorn juice. The preservation number of the strain is CGMCC NO: 33026. The strain is an adaptive evolutionary probiotic, can improve the activity and tolerance of the probiotic, has a good application effect in fermentation of sea-buckthorn juice, can maintain high bacterial activity, and has a good application prospect in fermentation of sea-buckthorn juice. According to the sea-buckthorn juice, the viable count of the sea-buckthorn juice is kept at 9.12-9.37 log (cfu / mL), compared with unfermented sea-buckthorn juice, the fermented sea-buckthorn juice has higher total phenol content and total flavone content, the antioxidant activity of the fermented sea-buckthorn juice is improved, the total phenol content can reach 545 mg / 100 mL and is increased by 95% compared with the unfermented sea-buckthorn juice, meanwhile, the method is a safe method without introduction of exogenous genes, can be applied to food, and has wide application prospects. Good application prospects are realized.

This invention discloses a culture medium for manganese-oxidizing bacteria suitable for low-carbon source environments in water supply networks and its application. The culture medium is prepared by mixing ferric ammonium citrate, manganese sulfate, ammonium sulfate, sodium chloride, magnesium sulfate, dipotassium hydrogen phosphate, and sodium nitrate, followed by high-temperature and high-pressure sterilization to obtain a culture solution. Manganese sulfate is added to the culture solution to obtain the manganese-oxidizing bacteria culture medium. Manganese-oxidizing bacteria are screened using this culture medium. Cast iron pipe scale is subcultured in the medium to enrich the manganese-oxidizing bacteria culture solution. The enriched manganese-oxidizing bacteria culture solution is diluted and spread onto agar solid medium, and incubated at a constant temperature to obtain manganese-oxidizing bacteria colonies. This invention solves the problem of altered bacterial physiological metabolism caused by high concentrations of added manganese ions, effectively improving bacterial activity and manganese oxidation capacity.

The application discloses a plant Lactobacillus rhamnosus microcapsule and a preparation method and application thereof, and the plant Lactobacillus rhamnosus is embedded by adopting a low-temperature spray drying process with lily starch, citrus pectin and whey protein as wall materials, and mainly includes the following steps: preparation of a bacterial suspension, preparation of a wall material solution, preparation of a microcapsule solution and low-temperature spray drying for preparing the microcapsule. The preparation technology of the stable encapsulated plant Lactobacillus rhamnosus microcapsule provided by the application has the advantages that the viable bacterial count in the microcapsule is high after spray drying, the microcapsule has strong gastrointestinal fluid resistance, is stable in storage, and can effectively solve the problem that the bacterial activity of the plant Lactobacillus rhamnosus and products thereof is reduced or even inactivated in the production and processing, storage and transportation and in-vivo digestion process; the prepared microcapsule can be used as a probiotic bacterial agent and applied to foods such as cheese, yogurt, ice cream, milk, chocolate, lactic acid bacterial beverages and baked products, and helps to promote the healthy development of the probiotic industry, and has obvious economic and social benefits.

The invention discloses a probiotic delivery system based on core-shell structure hydrogel and application of the probiotic delivery system, and belongs to the technical field of biological medicines.The probiotic delivery system based on the core-shell structure hydrogel comprises a hydrogel capsule shell and an inner core containing probiotics, the hydrogel capsule shell isolates the probiotics from the outside, and the inner core contains the probiotics; the hydrogel capsule comprises a shell and a core, the shell is made of sodium alginate, lipopolysaccharide generated by probiotics is adsorbed, the internal aperture of the shell of the hydrogel capsule is 200-300 nm, external nutrient substances are allowed to enter, metabolic substances secreted by the probiotics and having a treatment effect are allowed to go out, and the core is sodium alginate hydrogel which can well keep bacterial activity and contains certain nutrient substances required by bacteria. The probiotic delivery system has double isolation effects of physical isolation and chemical isolation, avoids negative effects of bacterial endotoxin on a human body, can realize bacterial isolation and drug permeation balance, and is good in long-term stability.

This invention outlines a method for creating a xanthone-enriched toothpaste using mangosteen peel and bamboo salt. This involves obtaining and preserving mangosteen peel, extracting xanthones from the peel, and incorporation with bamboo salt into a toothpaste formula. The product is designed for efficient absorption into gum cells during brushing, leveraging xanthones and bamboo salt to penetrate dental biofilm and absorption through oral mucosa, and provides antibacterial, anti-inflammatory, and antioxidant effects. Xanthones are proven to inhibit tooth decay and gum disease causing oral bacteria like Streptococcus mutans and Porphyromonas gingivalis. Mineral-rich bamboo salt adds therapeutic value to the toothpaste. The unique combination in the toothpaste helps target and combat oral health issues by reducing pro-inflammatory cytokines production, inhibiting harmful bacterial activity, and enhancing oral tissue health, with direct delivery to the mouth during brushing. This invention promotes oral health naturally by fighting harmful bacteria, reducing inflammation, and offering mineral enrichment.

The invention relates to the technical field of biological materials, and discloses a bacterial activity protective coating as well as a preparation method and application thereof. The bacterial activity protective coating is prepared from a methacrylic acid copolymer, a 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide compound and N-hydroxysuccinimide, and the bacterial activity protective coating is prepared from the methacrylic acid copolymer, the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide compound and the N-hydroxysuccinimide. According to the preparation method, carboxyl of polymethacrylate is activated through crosslinking of EDC and NHS, covalent linkage is formed with amino on cell walls of probiotics, and a stable and compact pH-responsive protective coating is constructed on the surfaces of bacteria. The coating is insoluble in a gastric acid environment, and can effectively protect probiotics from being damaged by harmful factors such as gastric acid; and the probiotics are released to play a role when the probiotics are dissolved in an intestinal weakly alkaline environment. The survival rate and functionality of the probiotics in gastrointestinal tracts are remarkably improved, and the probiotics have good biocompatibility and have no inhibition effect on growth and proliferation of the probiotics.

The present invention belongs to the field of antibacterial materials, and in particular relates to a piezoelectric photocatalytic antibacterial material and its preparation method and application. The method comprises: x Zn 1‑x A manganese source and a zinc source are separately mixed with an alkaline sodium compound solution and bentonite, and then subjected to low-speed ball milling and autothermal ball milling, respectively, to obtain the piezoelectric photocatalytic antibacterial material; wherein: 0<x<1. The piezoelectric photocatalytic antibacterial material prepared by the present invention has strong piezoelectricity and, while having high adsorption capacity, its positive charge interferes with bacterial activity, thereby achieving effective antibacterial and odor-removing effects. The preparation process is characterized by simple operation, low energy consumption, economical practicality, and a short production cycle.

This invention provides bfrA Single gene knockout or bfrA Genes and bfrB Double gene knockout of Neisseria gonorrhoeae and its applications involve the field of biotechnology. Research has found that... bfrA Single gene knockout and bfrA and bfrB Double gene knockout of Neisseria gonorrhoeae resulted in increased formation of vesicles on the outer membrane, disruption of the cell wall and cell membrane, reduced adhesion and invasion abilities, decreased bacterial activity, decreased ATP levels, and weakened ability to infect mice. Therefore, knocking out Neisseria gonorrhoeae... bfrA Gene or bfrA and bfrB The dual-gene assay can be used to prepare products for the production of gonococcal outer membrane vesicles and to construct low-pathogenic gonococcal models.

The present invention provides: a composition and a food / beverage item which can promote the elongation of pili of an intestinal bacterium and exhibits an anti-bacterial activity; and a method for assisting the examination and diagnosis of a disease caused by a pathogenic bacterium or a pathogenic fungus. The present invention provides a composition containing a bacterium capable of producing 3-phenylpropionic acid (PPA) or 3-(4-hydroxyphenyl)propionic acid (4OHPPA). The present invention also provides a food / beverage item containing PPA or 4OHPPA. The present invention further provides a method for assisting the examination and diagnosis of a disease caused by a pathogenic bacterium or a pathogenic fungus. The method comprises: quantifying the amount of PPA or the amount of 4OHPPA in feces from a subject by using 2-nitrophenylhydrazine; and comparing a value obtained by the quantification of the amount of PPA or the amount of 4OHPPA with a reference value. When the value obtained by the quantification of the amount of PPA or the amount of 4OHPPA is smaller than the reference value, it is determined that the subject is possibly affected by the disease.

The invention relates to a method for producing astaxanthin through fermentation of phaffia rhodozyma, and belongs to the technical field of microbial fermentation. The invention provides a method for producing astaxanthin by fermenting phaffia rhodozyma, which specifically comprises the following steps: inoculating an activated phaffia rhodozyma single strain into a seed culture medium, culturing to obtain a primary seed solution, inoculating the primary seed solution into the seed culture medium, culturing to obtain a secondary seed solution, inoculating into a fermentation culture medium, and fermenting at 20 DEG C to obtain the astaxanthin. Centrifuging, discarding supernatant, cleaning thalli with normal saline, performing wall breaking to obtain wall-broken liquid, and extracting to obtain astaxanthin. According to the method, seed culture is performed twice to improve the strain activity and enhance the metabolic activity, fermentation is performed on this basis to promote the synthesis and stability of astaxanthin, so that the phaffia rhodozyma thalli with higher astaxanthin content are obtained, and the phaffia rhodozyma thalli are prepared into liquid preparations or dry powder preparations to be applied to aquaculture.

This invention provides a photovoltaic-powered microbial fuel cell constructed wetland system, relating to the field of water pollution control. The system consists of a photovoltaic power supply module, a pulse modulation unit, a mode switching unit, a microbial fuel cell constructed wetland body, an auxiliary anode module, and an electrically isolated stimulation electrode pair. Through photovoltaic power supply and low duty cycle intermittent constant current pulse modulation, it can maintain stable operation for more than 72 hours under no-light conditions. Relying on the time-sharing pulse output of the mode switching unit, electrochemical oxidation of recalcitrant organic matter is achieved through the auxiliary anode. The electroisolated electrode pair stimulates the metabolism of electrogenic bacteria with a non-contact alternating electric field, solving the problems of unstable power supply and difficulty in balancing oxidation efficiency and bacterial activity in off-grid scenarios, thus improving wastewater treatment efficiency and long-term operational stability of the system.