Callicarpa nudiflora as fish vaccine adjuvant and application thereof

The technology of naked flower purple pearl and fish is applied in the field of naked flower purple pearl as an adjuvant for fish vaccine, which can solve the problems of large consumption of vaccine, high cost, difficult to handle, etc., and achieves improvement of immune protection rate, low residue and price. low cost effect

Pending Publication Date: 2022-05-31
HUNAN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These three vaccination methods have their own advantages and disadvantages: (1) Injection immunization can lead to the best immune protection, but the requirements for vaccination equipment are high, it is not suitable for fry or small fish, and it is labor-intensive; (2) The bathing operation is convenient, There is no need to transfer the fish out of the breeding pond, and it will not cause damage to the fish, but its disadvantage is that it is only suitable for fry or juveniles, and the cost will be too high if the adult fish or a large number of fish are immunized by bathing; (3) Oral Immunization is suitable for large numbers of fish that are difficult to handle or are prone to stress, with the disadvantage of poor protection and excessive vaccine consumption
At present, there is no relevant report on the use of nudibranchs as fish vaccine adjuvants

Method used

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  • Callicarpa nudiflora as fish vaccine adjuvant and application thereof
  • Callicarpa nudiflora as fish vaccine adjuvant and application thereof
  • Callicarpa nudiflora as fish vaccine adjuvant and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0023] (3) Preparation and use of inactivated vaccine: Put Edwardella lentus in 1% concentration formalin, and let stand overnight in a 4°C refrigerator. The next day, the cells were washed three times with PBS and centrifuged, and PBS was added to prepare inactivated vaccines of different concentrations. The zebrafish were inoculated by intraperitoneal injection after mixing different concentrations of the violet pearl adjuvant and the inactivated vaccine in proportion, and then the zebrafish were fed and observed according to the aforementioned method.

[0024] (4) Determination of the optimal concentration of S. japonica as an adjuvant for zebrafish / herring: different concentrations of S. japonica adjuvant (0mg / mL, 0.1mg / mL, 0.5mg / mL, 1mg / mL, 2.5mg / mL, 5mg / mL) and vaccine (10 4 / 10 5 cfu) immunized with zebrafish / herring after mixing in proportion, 30 days later, live Edward lentus challenge test was performed at a challenge concentration of 10 4 / 10 5 cfu, zebrafish / he...

Embodiment 1

[0026] Example 1: Test of safe injection dose of Violet nudica to zebrafish

[0027] 1. Materials and methods

[0028] (1) Test materials and breeding conditions: Five-month-old healthy TU strain zebrafish with a body weight of about 0.32 g were selected. During the test, each group was placed in a separate water tank, and the zebrafish were fed with Artemia twice a day, and the water temperature was controlled at 21 Between ℃-25℃, 14 hours of light and 10 hours of darkness per day;

[0029] (2) Determination of the safe dose for zebrafish injection with S. japonica: The zebrafish were injected intraperitoneally with S. japonica at the aforementioned concentration, 30 animals in each group, 10 μL per tail, and observed for 21 days.

[0030] 2. Test results

[0031] The death situation of zebrafish in the control group and the experimental group in 21 days is shown in Table 1. It can be seen that the maximum safe injection dose of zebrafish to zebrafish is 50 μg / tail, that is...

Embodiment 2

[0034] Example 2: Lethal concentration test of Edwardsiella lentus to zebrafish

[0035] 1. Materials and methods

[0036] (1) test material and breeding conditions: the same as in Example 1;

[0037] (2) Determination of lethal concentration of Edwardsiella lentus to zebrafish: 0 and 10 were used respectively. 3 , 10 4 , 10 5 , 10 6 , 107 The number of Edwardsiella lentus was intraperitoneally injected into zebrafish, 30 tails per group, 10 μL per tail, and observed for 21 days.

[0038] 2. Test results

[0039] The mortality of the zebrafish in the control group and the experimental group in 21 days is shown in Table 2. It can be seen that the injection of Edwardella lentus to the zebrafish has a lethal concentration of 10 4 / tail.

[0040] Table 2 Lethal concentration test of Edwardella lentus to zebrafish injection

[0041]

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PUM

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Abstract

The callicarpa nudiflora is used as the adjuvant of the fish vaccine, the effective component of the callicarpa nudiflora is callicarpa nudiflora, the application of the adjuvant can improve the specific protection rate after vaccination, and the adjuvant is used by injection after being mixed with an edwardsiella tarda inactivated vaccine. The callicarpa nudiflora is used as the fish vaccine adjuvant, and has the advantages of low price, low residue, no drug resistance of fish and the like.

Description

technical field [0001] The present invention relates to the field of biotechnology, in particular to the use of Violet violacea as a fish vaccine adjuvant and its application. Background technique [0002] In recent years, with the improvement of the scale and intensification of the aquaculture industry in my country, diseases have become an important factor restricting the sustainable and healthy development of the aquaculture industry. Among them, bacterial diseases caused by Edwardsiella tarda pose a huge threat to the healthy breeding of carp such as herring and grass carp. Edwardsiella tarda belongs to the Enterobacteriaceae genus Edwardsiella and is a gram-negative bacterium. It has been listed as three types of animal diseases by the Ministry of Agriculture and Rural Affairs of China. Its susceptible animals include fish, shellfish, and mammals. , reptiles, etc. Fish are primarily infected by ingestion of E. lentus-laden bait, or exposure to E. lentus-laden fish. E...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61K39/02A61P31/04
CPCA61K39/39A61K39/0208A61K9/0019A61P31/04A61K2039/55588A61K2039/54A61K2039/521Y02A50/30Y02A40/81
Inventor 冯浩吴慧肖军肖俊冯星刘吉晏俊
Owner HUNAN NORMAL UNIVERSITY
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