68results about How to "High immune protection rate" patented technology

The invention discloses a fish compound traditional Chinese medicinal preparation prepared from raw materials including rhubarb, acanthopanax, radix astragali, hawthorn and liquorice according a certain weight ratio. A method for preparing fish compound traditional Chinese medicinal preparation comprises the following steps of: A. washing and drying the rhubarb, the acanthopanax, the radix astragali, the hawthorn and the liquorice in the sun; B. respectively crushing and sieving the rhubarb, the acanthopanax, the radix astragali, the hawthorn and the liquorice to ensure the granularity reaches 140-200 mu; and C. weighing up the rhubarb, the acanthopanax, the radix astragali, the hawthorn and the liquorice according to the ratio and mixing and subpackaging after uniformly mixed to obtain the fish compound traditional Chinese medicinal preparation. The method has the advantages of feasibility, convenient operation, reasonable formulation and convenient use; traditional Chinese medicines have wide sources, low price, remarkable effects and no toxic or side effects; various components mutually act; and the invention simultaneously has two properties of nutrition and medicament, not only contains nutrient substances, but also has components with antibacterial activity and other bioactivities, can improve the metabolism of cultivated fishes, promote the growth and the development, improve the immunologic function, prevent and treat diseases and the like.

The invention belongs to the field of biological pharmacy. The invention provides a recombinant vaccine, comprising a SPV and one pharmaceutically acceptable vector and/or adjuvant or a plurality of such vectors and/or adjuvants. The SPV comprises an SPV vector and the encoding genes of SzP. The recombinant SPV vaccine provided in the invention can proliferate in large quantities in immune animalbodies, express target protein SzP and induce the generation of high titer antibodies in animal bodies, and exerts a good protective effect on immune animals.

The invention discloses recombinant porcine reproductive and respiratory syndrome virus (PRRSV) virus-like particles (VLP) and a preparation method and an application thereof. Based on comparative analysis of GP5 of a PRRSV epidemic strain and an M gene sequence, a GP5 and M tandem sequence GP5M is synthesized artificially, the synthesized GP5M gene sequence is cloned into a vector with a pBAC5 plasmid as a skeleton, the baculovirus transfer vector pBAC-PRRSVGP5M is obtained, the recombinant bacmid rBacmid-GP5M is obtained, sf9 cells are transfected with the bacmid, and the recombinant baculovirus Ac-PRRSVGP5M is obtained. The PRRSV GP5 and M protein are expressed efficiently by the recombinant baculovirus, and the virus-like particles are formed. A subunit vaccine prepared by the proteinexpressed by the recombinant baculovirus can induce a body to produce a specific immune response after immunizing animals and can protect the pig body against the strong poison attacking of porcine reproductive and respiratory syndrome virus.

The preparation process of reovirus antigen subunit vaccine for grass carp includes the following steps: proliferation of GCRV873, which is the Hunan Shaoyang strain of reovirus, in grass carp kidney cell line; centrifugal purification and concentration of GCRV873 infected cell virus suspension; processing purified virus with surfactant to disintegrate complete virus structure to form viral nucleic acid and capsid protein subunit component; digesting with nuclease to degrade free virus genome dsRNA; dialysis to obtain reovirus protein subunit antigen preparation for grass carp containing no nuclease; and diluting with physiological saline to obtain the said vaccine. The vaccine of the present invention has the advantages of containing complete virus protein antigen component, containing no genetic virus matter RNA, simple preparation process, high product purity, etc.

The invention discloses a method for screening candidate bacterial strains from fish streptococcus agalactiae vaccines. The method comprises the steps: separation and breed conservation of tilapia streptococcicosis agalactiae epidemic strains, identification of the epidemic strains and the establishing of a pathogenic library, PFGE genotype analysis of epidemic strains, toxicity determination of PFGE genotype representative strains, and immunogenicity and protection domain test of the PFGE genotype representative strains so as to obtain a candidate bacterial strains combination of a tilapia streptococcicosis agalactiae immunoprophylaxis vaccine in China. The technology has the characterizes of strong pertinence, high screening efficiency, remarkable effect and the like, and the screen candidate bacterial strains combination of the tilapia streptococcosis agalactiae immunoprophylaxis vaccine in China can protect 90% of genotypes and 96.47% of epidemic strains in the pathogenic library. The technology provides a new method for screening the candidate bacterial strains from the fish streptococcus agalactiae vaccines, is suitable for screening the candidate bacterial strains from the fish streptococcus agalactiae vaccines, and has significance and using value in immune prevention and control on fish streptococcicosis.

The invention provides Japanese encephalitis (JE) vaccine composition. The JE vaccine composition comprises a JE virus antigen of immunization effective quantity and water based vaccine adjuvant. The invention further provides a preparing method and application of the JE vaccine composition. The vaccine composition has good immunogenicity and immunizing protection. The immune effect of the JE vaccine composition is remarkably superior to that of single pig JE live vaccine and other live vaccine diluted by immunologic adjuvant. The clinical application prospects are good.

An attenuated live vaccine of eel vibrio for preventing the sensitive fish from being infected by wild vibrio and its preparing process are disclosed.

The invention relates to the field of immunology, and specifically relates to an Edwardsiella tarda subunit vaccine and a preparation method thereof. The protein gene of the vaccine has a base sequence shown in SEQ ID No.1 in a sequence table, and the vaccine protein coded by the protein gene has an amino acid sequence as shown in SEQ ID No.2 in the sequence table. The subunit vaccine provided by the invention is mixed with an oil emulsion adjuvant, and then capable of effectively exciting the generation of specific antibodies after immunizing the fish through intraperitoneal injection; the Edwardsiella tarda subunit vaccine is capable of efficiently improving the ability of resisting the infection of the Edwardsiella tarda of the immune fish and thus can be applied to controlling the Edwardsiella tarda of the fish.

Part of protein fragment G2 of MSRV glycoprotein is selected, G2 protein is obtained through prokaryotic recombinant expression, and mannosylation modification is carried out on the G2 protein to prepare a perch rhabdovirus nano-carrier targeting vaccine, and research shows that for perch after immunized by the subunit vaccine for 21 days is attacked by an MSRV FJ985 strain, the perch immune protection rate is 94%-96%, and the vaccine is safe and effective. After the perch is immunized, a better protection effect can be achieved, and the perch can effectively resist the attack of MSRV virulent virus.

The invention discloses an immune-enhanced recombinant PRRSV (Porcine Reproductive and Respiratory Syndrome Virus)-like particle subunit vaccine. The immune-enhanced recombinant PRRSV virus-like particle subunit vaccine consists of PRRSV-like particles and a compound immunologic adjuvant. The immune-enhanced recombinant PRRSV-like particle subunit vaccine disclosed by the invention has the beneficial effects that by the means of gene engineering, PRRSV GP5-M gene is modified, and the PRRSV-like particles with high immunogenicity are prepared by constructing rhabdovirus expression vectors. In addition, by improvement on the immunologic adjuvant, the immune-enhanced recombinant PRRSV-like particle subunit vaccine is obtained and the subunit vaccine has a better immune effect.

The invention relates to a preparation method and application of a Vibro harveyi and Vibrio parahaemolyticus bigeminy DNA vaccine. The Vibro harveyi and Vibrio parahaemolyticus bigeminy DNA vaccine is characterized by being a tdh2-oppA fusion gene protein eukaryotic expression engineering vector vaccine formed by connecting a Vibro harveyi oppA gene with a Vibrio parahaemolyticus tdh2 gene through 6 nucleotides GAATTC. The preparation method comprises the steps of: cloning the tdh2 gene and the oppA gene into a linear vector Pmd19-T Simple; inserting the fusion gene into a eukaryotic expression vector pEGFP-N1 by adopting a double-enzyme cleavage technology to construct a pEGFP-N1-tdh2-oppA fusion gene protein eukaryotic expression engineering vector vaccine; and finally preparing a Vibro harveyi and Vibrio parahaemolyticus bigeminy DNA vaccine suspension by using a conventional method, and immunizing fishes by the dosage of 100 microlitres per fish. The invention has strong operability, high repetition rate, safety, no toxic or side effect, double immune protection for fishes and higher immune effect than an inactivated vaccine, a recombinant protein vaccine and a common DNA vaccine.

The invention discloses recombined enterotoxin (namely, fimbriae composite polyvalent vaccine) for resisting diarrhea of piglets and a preparation method for the recombined enterotoxin (namely, the fimbriae composite polyvalent vaccine), and belongs to the technical field of animal husbandry and veterinary. The composite vaccine comprising an enterotoxin protein antigen and a various fimbriae antigen protein is prepared by the following steps of: uniformly emulsifying recombined trivalent enterotoxin SLS proteins expressed by gene engineering recombination and fimbriae antigen proteins of the conventional pathogenic bacteria K88, K99 and F41 in China with an adjuvant. The recombined enterotoxin (namely, fimbriae composite polyvalent vaccine) comprises two important pathogenic factors of an ETEC (enterotoxigenic e.coli) pathopoiesia loop, namely, enterotoxin and fimbriae; each process of diarrhea generated by the ETEC is protected; in the first step, the growth of the ETEC is stopped; in the second step, the enterotoxin generated by a small amount of growing ETEC is neutralized, so that the generation of the diarrhea is controlled from respective layers; the vaccine can not be limited by domains; the use range is relatively wide; and the protection rate is relatively high. After the vaccine is used, the protection rate is up to 95 percent; the immune efficiency of the vaccine is 5 times higher than that of the vaccine used in the market; and the recombined enterotoxin (namely, the fimbriae composite polyvalent vaccine) is safe and reliable.

The invention relates to a vaccine composition for improving the immune protective rate of anti-Fasciola hepatica Cat L1 (FhCat L1) DNA. The vaccine composition is pVAX1-Cat L1+pIL4+pFlt3L. An FhCat L1 DNA plasmid is constructed and a host body Th2 immune response is utilized to well kill or inhibit worm infection, and Flt3L which can induce the maturity of dendritic cells (DCs) and increase the number and the frequency of the DCs and a cell factor IL4 which can induce the host immune response trending to Th2 are selected as a molecular adjuvant to improve the immunogenicity and the immune protective rate of FhCat L1 DNA after the immunization of BALB/c mice, so a new idea is provided for the research and development of an anti-Fasciola hepatica DNA vaccine.

The invention provides a vibrio paraheamolyticus bivalent DNA vaccine as well as a preparation method and application thereof. The vibrio paraheamolyticus bivalent DNA vaccine is characterized in that a vibrio paraheamolyticus tdh2 gene and a vps gene are connected through 6 nucleotides to form a tdh2-vps fusion gene protein eukaryotic expression engineering vector vaccine. The preparation method of the vibrio paraheamolyticus bivalent DNA vaccine comprises the following steps of connecting the gene tdh2 and the gene vps by utilizing a double digestion technology after a cohesive end is obtained, and cloning into a prokaryotic expression vector pET28a to construct a fusion gene protein prokaryotic expression engineering vector; through the double digestion technology, inserting the fusion gene into a eukaryotic vector to construct a fusion gene protein eukaryotic expression engineering vector; finally, using a conventional method to prepare a vibrio paraheamolyticus bivalent DNA vaccine suspension. The invention has the advantages of strong operability, high repetition rate, safety without toxic and side effects, double immune protection on fish and higher immune effect than that of an inactivated vaccine, a recombinant protein vaccine and a monovalent DNA vaccine.

The invention provides a method for improving immune protective rate of a fasciola hepatica Cat L1 (FhCat L1) DNA (Deoxyribose Nucleic Acid) vaccine. The method is carried out based on the presenting effect of the antigen in dendritic cells, the influential role mechanism that the single-chain antibody scFvNLDC-145 of the DEC-205 of surface molecules of dendritic cells (DC) can target the DC cells is utilized, so that the resistance of a Spragae-Dawley rat to fasciola hepatica infection subjected to the immune encoding of the DNA vaccine of the FhCat L1 is improved, and as a result, the immune protective rate of the DNA vaccine of the FhCat L1 is improved.

The invention discloses a trachinotus ovatus-derived streptococcus agalactiae DNA vaccine as well as a preparation method and application thereof. The nucleotide sequence of the vaccine is shown as SEQ ID NO: 1, and the preparation method of the vaccine comprises the following steps: designing primers according to an internalin gene sequence, carrying out PCR (Polymerase Chain Reaction) amplification, connecting the obtained product with pMD-18T, transforming DH5alpha, selecting positive bacterium clones, carrying out amplification culture, extracting plasmids, and verifying to obtain pMD-internalin recombinant plasmids; performing enzyme digestion with restriction enzymes EcoR I and Xho I on the obtained plasmids and pcDNA3.1 (+) respectively, ligation of enzyme digestion products with T4DNA ligase and transformation of DH5alpha cells, selecting positive bacterium clones, extracting plasmids and obtaining the trachinotus ovatus-derived streptococcus agalactiae DNA vaccine through verification. The vaccine disclosed by the invention is relatively high in immune protection rate and relatively long in immune protection period.

The invention relates to application of a bacillus pumilus and immunopotentiator powdery preparation to preparation of biological agents for preventing and treating aquatic bacterial diseases. The bacillus pumilus and immunopotentiator powdery preparation comprises effective components of bacillus pumilus and an immunopotentiator, wherein the bacillus pumilus is a powdery bacterial agent prepared through solid fermentation or liquid fermentation of bacillus pumilus with the collection number of CCTCC M 2013240; the viable count of the bacillus pumilus is 109-1011cfu/g; the immunopotentiator comprises vitamin C, astragalus membranaceus, eucommia, codonopsis pilosula, Chinese angelica, honeysuckle, radix bupleuri, black plum, pomegranate peel and fructus schisandrae. The bacillus pumilus and immunopotentiator powdery preparation has the advantages as follows: the bacillus pumilus and immunopotentiator powdery preparation can significantly improve the resistance of fishes to infection of aeromonas hydrophila, enhance the body immunity of cultured fishes, significantly inhibit the growth of intestinal pathogenic bacteria of the fishes, promote the rapid growth of the fishes and improve the weight gain rate and the disease resistance of the cultured fishes.

The invention relates to a bacillus pumilus and immunopotentiator powdery preparation. The bacillus pumilus and immunopotentiator powdery preparation comprises effective components of bacillus pumilus and an immunopotentiator, wherein the bacillus pumilus is a powdery bacterial agent prepared through solid fermentation or liquid fermentation of bacillus pumilus with the collection number of CCTCC M 2013240; the viable count of the bacillus pumilus is 109-1011cfu/g; the immunopotentiator comprises vitamin C, astragalus membranaceus, eucommia, codonopsis pilosula, Chinese angelica, honeysuckle, radix bupleuri, black plum, pomegranate peel and fructus schisandrae. The bacillus pumilus and immunopotentiator powdery preparation has the advantages as follows: the bacillus pumilus and immunopotentiator powdery preparation can significantly improve the resistance of fishes to infection of aeromonas hydrophila, enhance the body immunity of cultured fishes, significantly inhibit the growth of intestinal pathogenic bacteria of the fishes, promote the rapid growth of the fishes and improve the weight gain rate and the disease resistance of the cultured fishes.