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Application of bacillus pumilus and immunopotentiator powdery preparation

A technology of Bacillus pumilus and immune enhancer, which is applied in the field of microorganisms to achieve the effects of inhibiting growth, improving infection ability, increasing weight gain rate and disease resistance

Inactive Publication Date: 2015-03-11
SUZHOU IRIVET BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there is no report about a kind of Bacillus pumilus that can significantly prevent and treat aquatic bacterial diseases and improve the survival rate and the powdery preparation of immune enhancer.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1 Bacillus pumilus liquid fermentation

[0020] The bacillus pumilus strain is cultivated in the order of slant, liquid seed and liquid fermentation, the slant medium is a conventional nutrient agar medium, and the liquid seed medium is a conventional nutrient broth liquid medium; liquid fermentation medium formula (W / V ): Glucose 2%, bean cake powder 8%, corn steep liquor 1%, MgSO 4 ·7H 2 O0.02%, NaCl 0.4%, KH 2 PO 4 0.1%, MnCl 2 0.04%, the fermentation temperature is 30°C, and the ventilation is cultivated for 24-48h. Obtain a high-concentration fermented broth, in which the number of viable bacteria is 10 9 -10 11 cfu / g. Centrifuge the fermented bacterial liquid to remove the fermented liquid to obtain wet bacterial sludge, add heat-resistant protective agent, and then spray dry to obtain solid powder bacterial agent with a viable count of 10 9 -10 11 cfu / g.

Embodiment 2

[0021] Example 2 Solid state fermentation of Bacillus pumilus

[0022] The Bacillus pumilus strain is cultivated in the order of slant, liquid seed and solid-state fermentation, the slant medium is conventional nutrient agar medium, and the liquid seed medium is conventional nutrient broth liquid medium. Inoculate the seed culture liquid with 5-10% inoculation amount into the solid-state fermentation medium for solid-state fermentation. The fermentation time is 24-48 hours, the fermentation temperature is 30°C, and the fermentation product is dried and pulverized to obtain Bacillus pumilus powdery bacteria agent. Among them, the number of live bacteria is 10 9 -10 11 cfu / g.

[0023] Wherein the solid-state fermentation medium is made up of material and water, and described material comprises rice bran 10%, bran 25%, soybean meal 32%, corn flour 30%, yeast extract 2%, NaCl 1 %, the ratio of material to water is 1:1.

Embodiment 3

[0024] The preparation of embodiment 3 powder preparation

[0025] The described Bacillus pumilus is solid-state fermented to make a powdery inoculum, and the number of viable Bacillus pumilus in the powdery inoculum is 10 9 -10 11 cfu / g, 16 parts of the powdered bacterial agent, 12 parts of vitamin C, 8 parts of astragalus, 5 parts of Eucommia, 4 parts of Codonopsis, 5 parts of angelica, 8 parts of honeysuckle, 10 parts of Bupleurum, 5 parts of black plum, pomegranate 6 parts of skin, 10 parts of schisandra. After being pulverized and passed through a 100-mesh sieve, the powder preparation is obtained by mixing evenly according to the proportion by weight.

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Abstract

The invention relates to application of a bacillus pumilus and immunopotentiator powdery preparation to preparation of biological agents for preventing and treating aquatic bacterial diseases. The bacillus pumilus and immunopotentiator powdery preparation comprises effective components of bacillus pumilus and an immunopotentiator, wherein the bacillus pumilus is a powdery bacterial agent prepared through solid fermentation or liquid fermentation of bacillus pumilus with the collection number of CCTCC M 2013240; the viable count of the bacillus pumilus is 109-1011cfu / g; the immunopotentiator comprises vitamin C, astragalus membranaceus, eucommia, codonopsis pilosula, Chinese angelica, honeysuckle, radix bupleuri, black plum, pomegranate peel and fructus schisandrae. The bacillus pumilus and immunopotentiator powdery preparation has the advantages as follows: the bacillus pumilus and immunopotentiator powdery preparation can significantly improve the resistance of fishes to infection of aeromonas hydrophila, enhance the body immunity of cultured fishes, significantly inhibit the growth of intestinal pathogenic bacteria of the fishes, promote the rapid growth of the fishes and improve the weight gain rate and the disease resistance of the cultured fishes.

Description

technical field [0001] The invention relates to the field of microbes, in particular to the application of a bacillus pumilus and powdery preparation of an immune enhancer. Background technique [0002] Bacterial diseases have always been one of the important reasons that plague the development of aquaculture. In recent years, while the high-density and intensive production mode has promoted the increase of production in the aquaculture industry, it has also increased the chance, frequency and harm of bacterial diseases; in addition, drug resistance, drug residues and The destruction of the ecological environment has also become the focus of social attention. Therefore, finding alternative drugs to antibiotics and exploring new control methods have become one of the important research directions for aquatic disease control. [0003] Quorum sensing (QS for short) means that bacteria can spontaneously produce and release some specific signaling molecules, sense changes in th...

Claims

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Application Information

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IPC IPC(8): A23K1/16A23K1/18A61K36/79A61K36/736A61P37/04A61P31/04A61K31/375A61K35/742
Inventor 宋增福许维素
Owner SUZHOU IRIVET BIOTECH
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