14results about How to "Improve infection ability" patented technology

The invention belongs to the technical field of biology, and discloses a capsid protein mutant capable of improving the infection ability of AAV to eye tissues and application of the capsid protein mutant. AAVRFDGTERAA and RQYSDAVRAE are inserted into amino acid at the 587 site and amino acid at the 588 site, which are equivalent to that of wild type AAV2 virus capsid protein, so that capsid protein mutants AAV2-NE01 and AAV2-NE02 are obtained. The virus yield of AAV2-NE01 and AAV2-NE02 is 1.96 times and 1.20 times that of AAV2-WT, after intravitreal injection, cells and trabecular meshwork areas of NHP retina GCL, INL to ONL can be well infected, the infection positive rate is high, the fluorescence intensity is high, the penetrating power to the retina is high, and visual cell nucleuses and inner segments can be infected. The AAV vector has a good application prospect and is expected to be developed into the AAV vector for treating eye diseases.

The present invention relates to the technical field of genetic breeding of marine economic shellfish, and in particular to a breeding method for a new Vibrio-resistant variety of scorpion clams. Specifically, the method comprises the following steps: using a semi-lethal concentration of Vibrio parahaemolyticus to stress adult scorpion clams, recovering 15% of the surviving individuals, and selecting scorpion clams with an 8% seed retention rate to obtain Vibrio-resistant scorpion clams, fattening the scorpion clams in an indoor workshop to mature the gonads, and inducing the scorpion clams to produce heritable immune factors by inactivating Vibrio parahaemolyticus before inducing spawning; then inducing the scorpion clams to spawn, obtaining the Vibrio-resistant strain of the F1 generation of scorpion clams, and then rapidly cultivating the scorpion clams to sexual maturity by adopting the breeding and culture technology of "breeding in the north and raising in the south" for the F1 generation; thereafter, selecting scorpion clams by stress, enhancing the immunity of the offspring, and continuously breeding by the method of "breeding in the north and raising in the south" every year to obtain a new Vibrio-resistant variety of scorpion clams with stable genetics and excellent traits. The method of the invention can significantly improve the anti-vibrio ability and the aquaculture survival rate of razor clams, and improve the good variety preservation and supply capacity of the razor clams aquaculture industry.

The invention discloses an application of micropterus salmoides neuropeptide in preparation of a product for preventing and treating aeromonas hydrophila infection. The amino acid sequence of the micropterus salmoides neuropeptide is as shown in SEQ ID NO. 1. The micropterus salmoides neuropeptide provided by the invention is a novel antibacterial peptide, can obviously enhance the aeromonas hydrophila infection resistance of micropterus salmoides when being applied in vivo and in vitro, and provides a new technical choice for preventing and treating aeromonas hydrophila related diseases and realizing'antibiotic-free culture 'of micropterus salmoides; the survival rate of the largemouth bass in the largemouth bass fry stage can be increased, green breeding is promoted, and the method has a wide application prospect.

The invention belongs to the technical field of biological fermentation, and particularly relates to a bifidobacterium metagen, a bifidobacterium subgen and a bifidobacterium subgen and a bifidobacterium subgen and a bifidobacterium subgen and a bifidobacterium subgen and a bifidobacterium subgen and a bifidobacterium subgen and a bifidobacterium subgen and a bifidobacterium subgen and a bifidobacterium subgen and a bifidobacterium subgen and a bifidobacterium subgen preparation method and application. The preparation method of the secondary prebiotics and the prebiotics comprises the following steps: centrifuging the secondary prebiotics to obtain supernate and precipitate; the supernate is filtered, and the secondary prebiotics are obtained; and resuspending the precipitate by using a culture solution to obtain the prebiotics. The bifidobacterium metagen, the bifidobacterium subgen and the bifidobacterium pseudogen prepared by the preparation method provided by the invention can achieve the effect of preventing and treating animal bacterial infection without colonization of host intestinal tracts, and have the advantages of high living bacteria number, obvious effect, small side effect and high safety.

The invention belongs to the technical field of biological pharmacy, and provides a piceatannol nano delivery particle based on chitosan oligosaccharide as well as a preparation method and application of the piceatannol nano delivery particle. The piceatannol nano delivery particle disclosed by the invention is a chitosan oligosaccharide nano drug-loaded particle modified by hyaluronic acid and D-tyrosine; the chitosan oligosaccharide nano drug-loaded particles comprise a chitosan oligosaccharide nano particle carrier and piceatannol wrapped in the chitosan oligosaccharide nano particle carrier. The hyaluronic acid and the D-tyrosine are adopted to carry out functional modification on the chitosan oligosaccharide nano drug-loaded particles, the hyaluronic acid realizes targeted delivery of MRSA infected parts, and the D-tyrosine has a biological membrane dispersing capacity, destroys a biological membrane structure, promotes a drug to deeply permeate into a biological membrane and improves an MRSA infection resisting effect; the antibacterial and anti-biofilm activity of piceatannol to MRSA (Methicillin Resistant Staphylococcus Aureus) is enhanced, and stronger antibacterial ability is shown; the lung bacterial load can be obviously reduced, the inflammatory lung injury can be relieved, and the treatment effect of resisting MRSA infection can be enhanced.

The invention discloses preparation and application of an intravenous injection oncolytic virus capable of strengthening a radiotherapy curative effect, and belongs to the field of nano-drugs. According to the invention, PEI-Se-Se-PEG is utilized to modify the surface of an oncolytic virus with negative charges, so that an invisible oncolytic virus suitable for intravenous injection, which is called AD (at) PSSP for short, is developed. Compared with the prior art, through intravenous injection of the AD (at) PSSP, the circulation time of the oncolytic virus in blood can be remarkably prolonged, and the safety is improved; in addition, not only is the inhibition effect of radiotherapy on whole-body tumor growth effectively improved, but also lasting immune memory can be activated, and tumor recurrence can be inhibited.

The invention discloses a method for synthesizing 2-(aminomethyl)-1,3-thiazole-5-carboxylic acid methyl ester, and belongs to the technical field of drug intermediate synthesis. The synthesis method uses 2-methylthiazole-5-carboxylic acid as the initial raw material, and sequentially obtains the target compound 2-(aminomethyl)-1,3-thiazole-5-carboxylic acid methyl ester through the classical chemical reactions of esterification, bromination, single reduction, double Boc amination and de-Boc, with a purity of more than 97%, a total yield of up to 48.3%, good stability, and solves the problems of low yield, difficult purification, strong reagent taste, difficult availability of cyclization raw materials, and inappropriate amplification of the overall process in the synthesis of similar compounds in the prior art, while promoting the application of the target compound in the field of medicine.

The present application relates to the technical field of microorganism, and particularly relates to a streptococcus gallolyticus and application of the streptococcus gallolyticus in preparation of anti-salmonella infection preparation. Streptococcus gallolyticus The streptococcus gallolyticus can improve the resistance of a host to salmonella infection, and can be used for preparing probiotic preparation, functional feed additive or related health care product for preventing or assisting in treating salmonella infection.

The application provides a polypeptide for targeting and an AAV vector for gene targeting and expression. The AAV vector comprises a nucleotide sequence encoding a modified capsid protein of an AAV-8 type serum, wherein the modified capsid protein is inserted with the polypeptide between the 590th amino acid and the 591st amino acid of the AAV-8 type serum capsid protein. The application also provides a recombinant adeno-associated virus particle comprising the modified capsid protein. In addition, the application also discloses a construction method and application of the polypeptide, the AAV vector and the recombinant adeno-associated virus particle. Compared with a vector expressing an AAV-8 type serum capsid protein, a cell and a tissue infected with the AAV vector can obtain a stronger fluorescence effect, and in the case of using the same virus amount, the AAV-8 type serum has a better expression effect.

The invention discloses an application of a single base mutation of a bovine DDX58 gene in anti-viral infection, and belongs to the technical field of anti-viral infection prevention and treatment. The single base mutation of the bovine DDX58 gene provided by the invention can significantly affect the replication of RNA viruses and the expression of IFNBI. When the 858th amino acid of the DDX58 protein is asparagine, the expression of IFNBI is significantly increased, thereby inhibiting the replication of RNA viruses and improving the anti-viral ability; the site is highly linked to the anti-RNA virus infection ability; the single / base / amino acid mutation of the bovine DDX58 gene provided can be used as a linkage site for bovine resistance to RNA virus infection, and is used for the genotype breeding of anti-viral dairy cow varieties, thereby simplifying the breeding method and shortening the breeding time.

The invention belongs to the technical field of beverage processing, and relates to a fruit juice beverage, in particular to low-sugar nutritional fruit juice and a preparation method thereof. The fruit juice beverage is prepared from the following fruits in parts by weight: 50 to 60 parts of passion fruit, 200 to 220 parts of grapefruit pulp, 240 to 260 parts of apple blocks and 140 to 150 parts of banana sections.

The invention discloses an ScAAV vector for efficiently targeting aqueous humor outflow functional cells as well as a screening method and application of the ScAAV vector. The ScAAV vector with the dominant serotype and the promoter is selected through the screening method, target cells can be efficiently infected, therapeutic genes are efficiently expressed to form protein to induce the target cells, the shape of the target cells is shrunk, intraocular aqueous humor outflow is promoted, and intraocular pressure is effectively reduced.

The invention relates to a biomedical titanium alloy surface modification method, and belongs to the technical field of laser application. The method comprises the following steps: firstly, machining a groove-micro-ripple composite structure on the surface of a clean and polished nickel-titanium alloy material by using femtosecond laser; then, rich nanosheets are formed on the basis of the structure through alkali heat treatment, and a groove-micro ripple-nanosheet multistage micro-nano composite structure is obtained; then, the multi-stage micro-nano composite structure is put into an alkaline Tris buffer solution containing dopamine hydrochloride to be coated with a polydopamine coating; and finally, the multi-stage micro-nano composite structure coated with the polydopamine coating is placed in a silver nitrate solution to load silver ions, surface modification of the titanium alloy is completed, and the biological activity, the osseointegration capacity and the bacterial infection resistance of the titanium alloy are improved.

This invention relates to a culture medium for high-density serum-free suspension culture of BHK-21 cells and its application. The culture medium contains inorganic salts, amino acids, vitamins, energy substances, buffers, antioxidants, sterols, polyamines, additives, and indicators. The antioxidants include dihydrolipoic acid (DLA) and S-acetyl-L-glutathione. This invention provides a culture medium with clearly defined components and significant culture effects for serum-free suspension culture of BHK-21 cells. The added DLA and S-acetyl-L-glutathione, as a combination of antioxidants in the culture medium, enhance the antioxidant properties of the serum-free suspension medium while also improving the cell viability of BHK-21 cells cultured in the medium.