167results about How to "Improve infection ability" patented technology

The invention discloses a probiotic agent containing Lactobacillus plantarum and Bifidobacterium lactis, and its preparing method, and belongs to the technical field of feed microbes. The probiotic agent having the advantages of special sour fragrance, high number of contained live bacteria and improved degradation rate of proteins in the soybean meal, wheat bran and corn flour is prepared through the solid-state fermentation of soybean meal, wheat bran and corn flour by adopting Lactobacillus plantarum HM-10 and Bifidobacterium lactis V9; and the method has the characteristics of low production cost and simple operation. The adoption of mixed bacterium segment solid state fermentation can utilize the mutualism synergism of strains, and can improve the yield and the thallus concentration; and the probiotic agent utilizing the growth, propagation and metabolism of the Lactobacillus plantarum HM-10 in the early stage of fermentation can provide abundant nutrients and necessary anaerobic environment for the propagation of the Bifidobacterium lactis V9, so it is helpful for the growth of the Bifidobacterium lactis V9, and the number of Lactobacillus plantarum HM-10 live bacteria is increased.

The invention relates to pseudo-virus. The pseudo-virus comprises a plasmid for expressing envelope protein Spike, a reporter gene plasmid and a packaging helper plasmid, wherein the plasmid for expressing the envelope protein Spike contains a CBA promoter and a human optimized Spike expression sequence, and is used for driving the expression of the envelope protein Spike. The pseudo-virus disclosed by the invention can simulate the process of infecting cells by wild novel coronavirus, can be used for researching the relationship between virus and host cells, cloning a receptor of virus and replacing a traditional neutralization test, and is used for researching differential diagnosis of diseases, evaluation and screening of antiviral drugs, evaluation of vaccine immune effect and the like. The invention also relates to a packaging method of the pseudo-virus and a novel coronavirus virus drug evaluation system containing the pseudo-virus, wherein the pseudo-virus is used for infectingthe 293T monoclonal cell which overexpresses the human ACE2 gene under the condition of drug interference, and by detecting the expression quantity of a reporter gene in the pseudo-virus (such as fluorescence intensity detection), the effectiveness of the drug is preliminarily evaluated.

The invention belongs to the technical field of skin care products, and particularly relates to mask liquid with a repairing function and a preparation method thereof in order to solve the problems that in the prior art, flushing, red itching and slow skin healing are caused after a face operation, skin is dry, rough, loose and free of elasticity, pigmentation is likely to be generated, or scars are left and hard to heal. The mask liquid with the repairing function is prepared from, by weight, 1-15 parts of butanediol, 1-15 parts of propylene glycol, 0.1-1 part of xanthan gum, 0.1-1 part of EDTA, 0.1-1 part of allantoin, 45-96 parts of water, 3-20 parts of glucan, 0.1-2 parts of snail mucus extract, 0.01-0.3 part of drawing agent, 0.01-0.4 part of decoyl hydroximic acid, 0.5-3 parts of glycerol, 0.01-0.6 part of oligopeptides-1, 0.1-3 parts of the stem of noble dendrobium and 0.01-0.5 part of sodium hyaluronate. The preparation method of the mask liquid with the repairing function is further provided. All the components act together according to the matching relationship to achieve the functions of repairing, skin whitening and face skin regeneration promoting.

The invention relates to a tilapia immune potentiator and a preparation method thereof, and belongs to the technical field of an aquatic compound feed additive. The preparation method is characterized by comprising the following steps: fully mixing curcumin, glutamine, betaine and a carrier; and crushing the components into dry powder. The tilapia immune potentiator has the advantages of stable raw material source, simple preparation process, wide material selection range and low cost; starting from an immune mechanism of fish, Chinese herbal medicines and a western medicine-glutamine dipeptide are supplemented in the immune potentiator; and by means of combination of the traditional Chinese medicines and the western medicine, immunity of the fish is improved. By utilizing the tilapia immune potentiator, the immunity and oxidation resistance of the fish are improved, the capability of tilapia on resisting pathogenic streptococcus infection is strengthened, and growth of the tilapia is promoted.

The invention provides a fermented composition having a function for regulating and improving the immunity of a human body. The fermented composition is prepared from yeast beta-glucosan, functional oligosaccharide, probiotics and edible and medical food; and the edible and medical food is mainly prepared from the following raw materials in parts by weight: 10 to 45 parts of conifer cherry powder and 3 to 26 parts of cordyceps cephalosporium mycelia. The fermented composition is a novel fermented product and a fermented fungi bag for preparing enzymes; and the fungi bag is convenient to use and suitable for industrialized production and family-type production of various plant enzymes.

The invention discloses a repairing agent for damaged skin, which is composed of the following components in mass percentage: β-glucan 5-10; propylene glycol 10-20; golden chamomile extract 5-10; purslane extract 5‑10; Aloe Vera Extract 5‑10; Chitosan 2‑6; Panthenol 2‑6; Sodium Hyaluronate 0.2‑0.5; Oligopeptide‑10.2‑0.5; And through the addition of ingredients and stirring into a viscous liquid through continuous cooling, the preparation method of the repair agent is formed, so that the repair agent prepared can improve the immunity of the human body, repair damaged cells, repair capillaries, and reduce the damage of nerve ending sheaths in the skin. Threshold, replenish and maintain skin moisture and other three-dimensional multi-faceted skin repairs, so that the skin returns to a healthy state.

The invention discloses a preparation method of probiotics-fermented Chinese wolfberry concentrated slurry. The preparation method comprises the following steps of crushing fresh Chinese wolfberry, dried Chinese wolfberry and Chinese wolfberry powder, so as to obtain Chinese wolfberry slurry, wherein the content of soluble solids is controlled to 15 to 30%; treating by ultrasonic waves, standing and hydrolyzing by enzyme, sterilizing, and inoculating with probiotics; warming at the temperature of 20 to 45 DEG C, and fermenting; naturally stopping fermenting, or finishing fermenting when the pH(potential of hydrogen) value reaches 3.0 to 4.5, so as to obtain probiotics-fermented Chinese wolfberry raw juice; separating the probiotics-fermented Chinese wolfberry raw juice, so as to obtain probiotics-fermented Chinese wolfberry paste; mixing 10 to 50% of probiotics-fermented Chinese wolfberry raw juice and 0 to 10% of probiotics-fermented Chinese wolfberry paste, and adding a proper amount of honey, syrup or white granulated sugar, stabilizer, sodium citrate, essence, sodium erythorbate, oligosaccharide, lutein, taurine, zinc gluconate, Vc (vitamin c) and other nutrition enhancers, soas to obtain the probiotics-fermented Chinese wolfberry concentrated slurry. The obtained probiotics-fermented Chinese wolfberry concentrated slurry has the advantages that the rich nutrients are contained, the color is bright and attractable, the flavor is elegant, and the bioactivity is higher.

The invention discloses a method for improving the streptococcus iniae infection-resisting capacity of GIFT oreochromis niloticus during high-temperature stress. The method comprises the following steps: preparing basic ration and a compound Chinese medicinal herb formula, and feeding feed. Due to the adoption of the method, the immunity index of the GIFT oreochromis niloticus during high-temperature stress can be effectively increased, the liver damage is reduced, meanwhile, the streptococcus iniae infection-resisting capacity of the GIFT oreochromis niloticus can be improved, the morbidity is reduced, and the survival rate of the GIFT oreochromis niloticus infected with streptococcus iniae can be increased. Several Chinese medicinal herbs involved in the method are relatively available and relatively low in cost; a production technology is simple. The basic ration with the compound Chinese medicinal herbs has relatively good palatability, and after the basic ration is taken by the GIFT oreochromis niloticus, drug residues in bodies of the GIFT oreochromis niloticus are relatively few, and therefore, the GIFT oreochromis niloticus meets the requirements on green healthy aquatic products.

The invention discloses a ZIKV (zika virus) vaccine. The ZIKV vaccine contains or can express membrane protein prM/M, envelope protein E and non-structural protein NS1 of ZIKV. The invention also discloses a preparation method of the ZIKV vaccine. The ZIKV vaccine has higher immunogenicity and can more effectively prevent ZIKV infection.

The invention discloses a method for Agrobacterium-mediated genetic transformation of sugarcane growth points, and belongs to the technical field of sugarcane cultivation. The method comprises the following steps: selecting a genetically transformed sugarcane material, a strain and plasmid, preparing a callus induction medium M1, a differentiation medium M2, a rooting medium M3, a transformation medium MR and a co-medium M4, inducing and differentiating sugarcane callus, preparing an Agrobacterium engineering bacterial solution, using an Agrobacterium-mediated method to perform genetic transformation on the sugarcane growth points, and testing transgenic plants. The method shortens the time for sugarcane to obtain transformed plants, and improves the transformation efficiency. Through theAgrobacterium-mediated transformation technology, a pCAMBIA3301 plant expression vector is introduced into GT35 sterile seedlings, a total of 353 resistant plants are obtained after screening by a screening medium, the resistant plants are tested by PCR, 333 plants are positive, a positive rate reaches to 94.33%, and thereby the result indicates that the foreign gene is successfully introduced into sugarcane.

The invention discloses a method for earthing-cultivating silkworm insect grass by simulating an ecological environment. The method comprises the following steps: (1) selecting a strain host; (2) enlarging preparation solution of cultivated strain liquid; (3) preprocessing and postprocessing inoculation; (4) preparing an embedding substrate; and (5) management of earthing-cultivating: inoculatingthe strains optimized in the step (2) in a fluid nutrient medium added with 5-8 wt% of silkworm chrysalis meal, after enlarging the cultivation, inoculating in healthy silkworm larvae in the step (1)for infecting and rigidifying, and embedding infected and rigidified silkworm bodies to be developed in the step (3) in a transparent plastic box filled with the plant ash embedding substrate; after sporophore is grown to be matured, taking out the silkworm bodies from the plant ash embedding substrate, and cleaning up plant ash adhered to the periphery of the silkworm bodies, to obtain the matured silkworm insect grass. The method has the characteristics of low sundry fungus contamination rate, high infection rigidifying rate, high production rate, good stability of the obtained silkworm insect grass, higher yield, low cost, and full and regular appearance of the silkworm insect grass, is suitable for the large-scale production, and has the extensive development prospect.

The invention discloses a II-set alphabaculovirus shuttle expression vector MbBacmid capable of expressing foreign protein in various insect cells and application thereof. The Bacmid contains whole genome sequences of mamestra brassicae nuclear polyhedrosis viruses, and contains genetic elements capable of being copied in escherichia coli. Foreign protein genes can be quickly inserted into the Bacmid provided by the invention through a donor plasmid pFast-Bac Mbph, and are efficiently expressed inside various commercial insect cells including Sf9, Tn368, high five and the like. The vector and a donor plasmid pFast-Bac Mbph+piel-cath can be subjected to homologous recombination to obtain a recombinant virus MbMNPV-ph+ piel-cath capable of increasing insect killing speed. The expression system provided by the invention is the only II-set alphabaculovirus shuttle expression vector system capable of efficiently expressing foreign protein in various commercial insect cells.

The invention belongs to the technical field of healthcare products, and concretely relates to an immunity enhancing healthcare food prepared through mixing wall-broken Mythic Fungus spore powder with a Mythic Fungus extract. The healthcare product has the advantages of easy production, low cost, good mouthfeel, easy absorption, enhancement of the pathogen infection resistance of a body, and improvement of the health level of the body, and is a good immunity enhancing healthcare food.

The invention discloses a chimeric simian/human immunodeficency virus strain and application thereof, relating to a virus modified with an introduced exogenetic gene material. The virus is a simian/human immunodeficency virus SHIV-XJN0363B8 carrying a China HIV-1 (Human Immunodeficiency Virus) B'/C recombinant subtype envelope protein gene segment, has the classification name as immunodeficency virus and the Latin name as Lentivirus, and is preserved with the collection number of CGMCC No. 4243 on Oct. 15th, 2010 in China General Microbiological Culture Collection Center with the address of No.3, yard 1, Beichen west road, Chaoyang District, Beijing. An SHIV-XJN0363B8/China rhesus monkey AIDS (Acquired Immune Deficiency Syndrome) model built by using the virus strain SHIV-XJN0363B8 can beused for effectively simulating the virus replication characteristic at the early stage of HIV-1 infection, and play roles in researching the in-vivo pathogenic mechanism of HIV-1 and evaluating the effectiveness of biological agents, such as AIDS vaccines, AIDS antiviral medicaments and the like, in China.

The invention relates to a medicine composite containing erythromycin thiocyanate and a preparation method thereof. The composite comprises erythromycin thiocyanate, stabilizers, solubilizer and auxiliary materials. The preparation method includes the steps that the stabilizers, the solubilizer and erythromycin thiocyanate are evenly mixed, and the mixture is screened by a screen of 80-120 meshes in a smashed mode to obtain a semi-finished product; the auxiliary materials are screened by a screen of 80-100 meshes in a smashed mode to be thrown into a mixing tank; then the semi-finished product is added, even mixing is performed, and the medicine composite is obtained through sub-packaging. The approximate solubility of the medicine composite containing erythromycin thiocyanate in water is remarkably improved, stability is good, and through six months of acceleration tests, all detection items meet quality standards. The water solution of the medicine composite is not likely to be affected by temperature, water quality hardness and other objective factors, the content of effective components in drinking water can be kept unchanged for 24 hours, exertion of the medicine effect is guaranteed, and the effect of resisting gram-positive bacteria and mycoplasma infection is remarkably improved for livestock.

The invention relates to a composite porcine alpha-IFN gene and a recombinant vector thereof, and belongs to the technical field of genetic engineering biological products. The newly designed gene sequences of composite porcine alpha-IFN are recombined to pPICZ alpha-A vectors, and the vectors are electronically transformed into yeast competent cells; and the positive identification, and the screening and the induced expression of high copy yeast strains are carried out. A pichia pastoris expression system is used for expressing foreign genes so as to be favorable for the commercial production of porcine IFN. Compared with the natural IFN which is expressed by yeast, the expression level of the protein which is expressed by genome increases by 3.17 times. The fluorescent quantitative PCR result shows that the level of PRVmRNA after PK-15 cells treated by 10-6mg/ml of the composite porcine alpha-IFN are re-infected with PRV is obviously lower than the level of PRVmRNA after the PK-15 cells which are not treated by the composite porcine alpha-IFN are infected with the PRV, and the level of PRVmRNA decreases by 37 times.

The invention provides application of cyclic dipeptide in preparation of drugs for treating skin injury. The cyclic dipeptide is cyclo-L-prolyl-L-dipeptide, and is particularly used for burns and scalds. The dipeptide has the features of small molecular weight, simple structure and the like. The cyclic dipeptide has a remarkable effect on treatment of burns and scalds, can quickly permeate injured skin tissues and enter cells in a treatment process, repairs injured cells and activates self-repairing capability, wound healing speed is accelerated, recurrence rate is low after healing, scars are shallow, and potential side effects after treatment by traditional Chinese medicines and western drugs are avoided. Moreover, the cyclic dipeptide also has effects of inhibiting inflammation and stopping pain, and improving immunity.

The invention relates to the field of proteins, and particularly relates to a bioactive peptide AGYDVEKNNSRIKLGLK and a preparation method and application thereof. The amino acid sequence of the bioactive peptide AGYDVEKNNSRIKLGLK is Ala-Gly-Tyr-Asp-Val-Glu-Lys-Asn-Asn-Ser-Arg-Ile-Lys-Leu-Gly-Leu-Lys. In-vitro immune function regulation experiments verify that the bioactive peptide AGYDVEKNNSRIKLGLK has a good immune regulation function. The bioactive peptide AGYDVEKNNSRIKLGLK can promote macrophage activation and release cell factors, has the capacity of promoting increase of macrophage nitric oxide induction amount under a certain concentration condition, improves the capacity of resisting external pathogen infection of the body, reduces the morbidity of the body, improves the life quality and has very important significance in developing food, health care products and medicine with the immune regulation function.