2results about How to "Improve infection ability" patented technology

The application provides a polypeptide for targeting and an AAV vector for gene targeting and expression. The AAV vector comprises a nucleotide sequence encoding a modified capsid protein of an AAV-8 type serum, wherein the modified capsid protein is inserted with the polypeptide between the 590th amino acid and the 591st amino acid of the AAV-8 type serum capsid protein. The application also provides a recombinant adeno-associated virus particle comprising the modified capsid protein. In addition, the application also discloses a construction method and application of the polypeptide, the AAV vector and the recombinant adeno-associated virus particle. Compared with a vector expressing an AAV-8 type serum capsid protein, a cell and a tissue infected with the AAV vector can obtain a stronger fluorescence effect, and in the case of using the same virus amount, the AAV-8 type serum has a better expression effect.

This invention relates to a culture medium for high-density serum-free suspension culture of BHK-21 cells and its application. The culture medium contains inorganic salts, amino acids, vitamins, energy substances, buffers, antioxidants, sterols, polyamines, additives, and indicators. The antioxidants include dihydrolipoic acid (DLA) and S-acetyl-L-glutathione. This invention provides a culture medium with clearly defined components and significant culture effects for serum-free suspension culture of BHK-21 cells. The added DLA and S-acetyl-L-glutathione, as a combination of antioxidants in the culture medium, enhance the antioxidant properties of the serum-free suspension medium while also improving the cell viability of BHK-21 cells cultured in the medium.