6results about How to "Improve infection ability" patented technology

The invention belongs to the technical field of biological pharmacy, and provides a piceatannol nano delivery particle based on chitosan oligosaccharide as well as a preparation method and application of the piceatannol nano delivery particle. The piceatannol nano delivery particle disclosed by the invention is a chitosan oligosaccharide nano drug-loaded particle modified by hyaluronic acid and D-tyrosine; the chitosan oligosaccharide nano drug-loaded particles comprise a chitosan oligosaccharide nano particle carrier and piceatannol wrapped in the chitosan oligosaccharide nano particle carrier. The hyaluronic acid and the D-tyrosine are adopted to carry out functional modification on the chitosan oligosaccharide nano drug-loaded particles, the hyaluronic acid realizes targeted delivery of MRSA infected parts, and the D-tyrosine has a biological membrane dispersing capacity, destroys a biological membrane structure, promotes a drug to deeply permeate into a biological membrane and improves an MRSA infection resisting effect; the antibacterial and anti-biofilm activity of piceatannol to MRSA (Methicillin Resistant Staphylococcus Aureus) is enhanced, and stronger antibacterial ability is shown; the lung bacterial load can be obviously reduced, the inflammatory lung injury can be relieved, and the treatment effect of resisting MRSA infection can be enhanced.

The invention discloses a method for synthesizing 2-(aminomethyl)-1,3-thiazole-5-carboxylic acid methyl ester, and belongs to the technical field of drug intermediate synthesis. The synthesis method uses 2-methylthiazole-5-carboxylic acid as the initial raw material, and sequentially obtains the target compound 2-(aminomethyl)-1,3-thiazole-5-carboxylic acid methyl ester through the classical chemical reactions of esterification, bromination, single reduction, double Boc amination and de-Boc, with a purity of more than 97%, a total yield of up to 48.3%, good stability, and solves the problems of low yield, difficult purification, strong reagent taste, difficult availability of cyclization raw materials, and inappropriate amplification of the overall process in the synthesis of similar compounds in the prior art, while promoting the application of the target compound in the field of medicine.

The present application relates to the technical field of microorganism, and particularly relates to a streptococcus gallolyticus and application of the streptococcus gallolyticus in preparation of anti-salmonella infection preparation. Streptococcus gallolyticus The streptococcus gallolyticus can improve the resistance of a host to salmonella infection, and can be used for preparing probiotic preparation, functional feed additive or related health care product for preventing or assisting in treating salmonella infection.

The application provides a polypeptide for targeting and an AAV vector for gene targeting and expression. The AAV vector comprises a nucleotide sequence encoding a modified capsid protein of an AAV-8 type serum, wherein the modified capsid protein is inserted with the polypeptide between the 590th amino acid and the 591st amino acid of the AAV-8 type serum capsid protein. The application also provides a recombinant adeno-associated virus particle comprising the modified capsid protein. In addition, the application also discloses a construction method and application of the polypeptide, the AAV vector and the recombinant adeno-associated virus particle. Compared with a vector expressing an AAV-8 type serum capsid protein, a cell and a tissue infected with the AAV vector can obtain a stronger fluorescence effect, and in the case of using the same virus amount, the AAV-8 type serum has a better expression effect.

The invention discloses an ScAAV vector for efficiently targeting aqueous humor outflow functional cells as well as a screening method and application of the ScAAV vector. The ScAAV vector with the dominant serotype and the promoter is selected through the screening method, target cells can be efficiently infected, therapeutic genes are efficiently expressed to form protein to induce the target cells, the shape of the target cells is shrunk, intraocular aqueous humor outflow is promoted, and intraocular pressure is effectively reduced.

This invention relates to a culture medium for high-density serum-free suspension culture of BHK-21 cells and its application. The culture medium contains inorganic salts, amino acids, vitamins, energy substances, buffers, antioxidants, sterols, polyamines, additives, and indicators. The antioxidants include dihydrolipoic acid (DLA) and S-acetyl-L-glutathione. This invention provides a culture medium with clearly defined components and significant culture effects for serum-free suspension culture of BHK-21 cells. The added DLA and S-acetyl-L-glutathione, as a combination of antioxidants in the culture medium, enhance the antioxidant properties of the serum-free suspension medium while also improving the cell viability of BHK-21 cells cultured in the medium.