169results about How to "Improve infection ability" patented technology

The invention relates to a new method for preparing an antibiotic bioactive coating material on the surface of titanium alloy and magnesium alloy by ultrasonic-microarc oxidation composite technology, which can be used for obtaining a biological coating material which is compact at the bottom layer and is porous at the surface layer, wherein Ca, P and Ag contained in the coating can improve the bioactivity and the corrosion resistance of magnesium and titanium, and reduce bacterial infection caused by implantation. The invention can meet the requirements of bearing bones of human beings for mechanical properties of implanted materials, and can overcome disadvantages of the traditional surface modification method for biologic materials. In the coating composite material, the coating thickness of titanium alloy is 50-85 mu m, the surface hole diameter is 4-25 mu m, the porosity is 20-30%, and the bonding strength between the coating and the matrix is 23-40 MPa. The coating thickness of magnesium alloy is 16-22 mu m, the surface hole diameter is 5-28 mu m, the porosity is 21-30%, and the bonding strength between the coating and the matrix is 8-20 MPa.

The invention relates to the field of gene therapy, in particular relates to sgRNA (singleguide Ribonucleic Acid), as well as a lentiviral vector constructed by the same and application thereof and specifically relates to sgRNA with SIVmac1A11 lentivirus as a framework to express SpCas9 protein and gene specificity. The sgRNA is applied to treating human and simian AIDS (Acquired Immune Deficiency Syndrome). A nucleotide sequence of the sgRNA is shown as SEQ ID NO.1 to 2. According to the sgRNA disclosed by the invention, a current most efficient CRISPR / Cas9 gene editing tool is utilized, a designed CXCR4 / CCR5 gene sgRNA locus has gene knockout activity superior to other loci reported by existing research, and the sgRNA is applied to gene therapy of SIV infected rhesus monkeys for the first time. Compared with ZFN and TALEN, the sgRNA has the advantages of being convenient to operate, low in cost and the like.

The invention discloses a method to mass produce flu vaccine with bioreactor, which comprises the following steps: proceeding passage adaptation on Africa green monkey kidney cell (Vero cell) with influenza virus; intensifying intrinsic appeal of influenza virus for Vero cell; fitting for Vero cell quickly; breeding the Vero cell with cell density above 10. 7deka / ml in bioreactor; seeding the influenza virus into bioreactor; adjusting various controlling parameter in the reactor; making the virus high effective breed in the reactor; proceeding continuous perfusion; harvesting virus liquid; deactivating the virus; condensing; purifying; cracking; getting the flu vaccine. This invention possesses the advantages of high antigen quantity and stable quality.

The invention discloses feed additive which improves the immunity of seawater fish. The feed additive consists of three or more than three types in clostridium butyricum, mannan oligosaccharide, xylo-oligosaccharide and fructooligosaccharide by proportion. 0.1 percent to 1 percent of seawater fish immunity intensifier of the invention is added into seawater fish feed, which can obviously improve the level of the muramidase in serum and skin mucus, obviously improve the level of IgM in the serum and the skin mucus, improve the fish immunity response capability, thereby improving the disease resistance of the cultivated fish. 1 percent of the immunity intensifier of the invention is added into the seawater fish feed, which can obviously improve the daily weight gain of the fish and reduce the food coefficient. The feed additive which improves the immunity of the seawater cultivating fish of the invention can obviously improve the immunity response level of the seawater cultivating fish, improve the disease resistance, and is safe without public harm and residue; the seawater fish cultivated by the additive meet the green food sanitation requirements.

The invention discloses a probiotic agent containing Lactobacillus plantarum and Bifidobacterium lactis, and its preparing method, and belongs to the technical field of feed microbes. The probiotic agent having the advantages of special sour fragrance, high number of contained live bacteria and improved degradation rate of proteins in the soybean meal, wheat bran and corn flour is prepared through the solid-state fermentation of soybean meal, wheat bran and corn flour by adopting Lactobacillus plantarum HM-10 and Bifidobacterium lactis V9; and the method has the characteristics of low production cost and simple operation. The adoption of mixed bacterium segment solid state fermentation can utilize the mutualism synergism of strains, and can improve the yield and the thallus concentration; and the probiotic agent utilizing the growth, propagation and metabolism of the Lactobacillus plantarum HM-10 in the early stage of fermentation can provide abundant nutrients and necessary anaerobic environment for the propagation of the Bifidobacterium lactis V9, so it is helpful for the growth of the Bifidobacterium lactis V9, and the number of Lactobacillus plantarum HM-10 live bacteria is increased.

The invention relates to pseudo-virus. The pseudo-virus comprises a plasmid for expressing envelope protein Spike, a reporter gene plasmid and a packaging helper plasmid, wherein the plasmid for expressing the envelope protein Spike contains a CBA promoter and a human optimized Spike expression sequence, and is used for driving the expression of the envelope protein Spike. The pseudo-virus disclosed by the invention can simulate the process of infecting cells by wild novel coronavirus, can be used for researching the relationship between virus and host cells, cloning a receptor of virus and replacing a traditional neutralization test, and is used for researching differential diagnosis of diseases, evaluation and screening of antiviral drugs, evaluation of vaccine immune effect and the like. The invention also relates to a packaging method of the pseudo-virus and a novel coronavirus virus drug evaluation system containing the pseudo-virus, wherein the pseudo-virus is used for infectingthe 293T monoclonal cell which overexpresses the human ACE2 gene under the condition of drug interference, and by detecting the expression quantity of a reporter gene in the pseudo-virus (such as fluorescence intensity detection), the effectiveness of the drug is preliminarily evaluated.

The invention belongs to the technical field of skin care products, and particularly relates to mask liquid with a repairing function and a preparation method thereof in order to solve the problems that in the prior art, flushing, red itching and slow skin healing are caused after a face operation, skin is dry, rough, loose and free of elasticity, pigmentation is likely to be generated, or scars are left and hard to heal. The mask liquid with the repairing function is prepared from, by weight, 1-15 parts of butanediol, 1-15 parts of propylene glycol, 0.1-1 part of xanthan gum, 0.1-1 part of EDTA, 0.1-1 part of allantoin, 45-96 parts of water, 3-20 parts of glucan, 0.1-2 parts of snail mucus extract, 0.01-0.3 part of drawing agent, 0.01-0.4 part of decoyl hydroximic acid, 0.5-3 parts of glycerol, 0.01-0.6 part of oligopeptides-1, 0.1-3 parts of the stem of noble dendrobium and 0.01-0.5 part of sodium hyaluronate. The preparation method of the mask liquid with the repairing function is further provided. All the components act together according to the matching relationship to achieve the functions of repairing, skin whitening and face skin regeneration promoting.

The invention discloses a green feed additive and a preparation method thereof. The green feed additive is prepared by mixing the following components in portion by weight: 40 to 60 portions of cortex eucommiae extract, 10 to 20 portions of streptococcus faecalis powder, 30 to 40 portions of polysaccharide peptide powder and 8 to 20 portions of folium cortex eucommiae finemeal, wherein the cortex eucommiae extract at least contains 0.2 percent of chlorogenic acid; the polysaccharide peptide powder is prepared by concentrating, decoloring, precipitating with alcohol and drying the ferment liquid which is obtained by fermenting the streptococcus faecalis powder. The components of the invention are integrally applied, which can remarkably improve the function of an animal immune system, strengthen disease preventing ability of organism, and resist the intrusion of pathogen; the green feed additive contains virus resisting the immunodeficiency which can bidirectionally adjust the function of cellular immunity and greatly improve the survival rate of feeding animals; and under the conditions without hormone, the green feed additive can promote the growth and breed of animals, greatly improve the quality and taste of eggs and meat, and keep the meat quality more fresh and more close to wild taste.

The invention relates to a tilapia immune potentiator and a preparation method thereof, and belongs to the technical field of an aquatic compound feed additive. The preparation method is characterized by comprising the following steps: fully mixing curcumin, glutamine, betaine and a carrier; and crushing the components into dry powder. The tilapia immune potentiator has the advantages of stable raw material source, simple preparation process, wide material selection range and low cost; starting from an immune mechanism of fish, Chinese herbal medicines and a western medicine-glutamine dipeptide are supplemented in the immune potentiator; and by means of combination of the traditional Chinese medicines and the western medicine, immunity of the fish is improved. By utilizing the tilapia immune potentiator, the immunity and oxidation resistance of the fish are improved, the capability of tilapia on resisting pathogenic streptococcus infection is strengthened, and growth of the tilapia is promoted.

The invention provides a fermented composition having a function for regulating and improving the immunity of a human body. The fermented composition is prepared from yeast beta-glucosan, functional oligosaccharide, probiotics and edible and medical food; and the edible and medical food is mainly prepared from the following raw materials in parts by weight: 10 to 45 parts of conifer cherry powder and 3 to 26 parts of cordyceps cephalosporium mycelia. The fermented composition is a novel fermented product and a fermented fungi bag for preparing enzymes; and the fungi bag is convenient to use and suitable for industrialized production and family-type production of various plant enzymes.

The invention discloses a repairing agent for damaged skin, which is composed of the following components in mass percentage: β-glucan 5-10; propylene glycol 10-20; golden chamomile extract 5-10; purslane extract 5‑10; Aloe Vera Extract 5‑10; Chitosan 2‑6; Panthenol 2‑6; Sodium Hyaluronate 0.2‑0.5; Oligopeptide‑10.2‑0.5; And through the addition of ingredients and stirring into a viscous liquid through continuous cooling, the preparation method of the repair agent is formed, so that the repair agent prepared can improve the immunity of the human body, repair damaged cells, repair capillaries, and reduce the damage of nerve ending sheaths in the skin. Threshold, replenish and maintain skin moisture and other three-dimensional multi-faceted skin repairs, so that the skin returns to a healthy state.

The invention discloses a preparation method of probiotics-fermented Chinese wolfberry concentrated slurry. The preparation method comprises the following steps of crushing fresh Chinese wolfberry, dried Chinese wolfberry and Chinese wolfberry powder, so as to obtain Chinese wolfberry slurry, wherein the content of soluble solids is controlled to 15 to 30%; treating by ultrasonic waves, standing and hydrolyzing by enzyme, sterilizing, and inoculating with probiotics; warming at the temperature of 20 to 45 DEG C, and fermenting; naturally stopping fermenting, or finishing fermenting when the pH(potential of hydrogen) value reaches 3.0 to 4.5, so as to obtain probiotics-fermented Chinese wolfberry raw juice; separating the probiotics-fermented Chinese wolfberry raw juice, so as to obtain probiotics-fermented Chinese wolfberry paste; mixing 10 to 50% of probiotics-fermented Chinese wolfberry raw juice and 0 to 10% of probiotics-fermented Chinese wolfberry paste, and adding a proper amount of honey, syrup or white granulated sugar, stabilizer, sodium citrate, essence, sodium erythorbate, oligosaccharide, lutein, taurine, zinc gluconate, Vc (vitamin c) and other nutrition enhancers, soas to obtain the probiotics-fermented Chinese wolfberry concentrated slurry. The obtained probiotics-fermented Chinese wolfberry concentrated slurry has the advantages that the rich nutrients are contained, the color is bright and attractable, the flavor is elegant, and the bioactivity is higher.

The invention discloses a method for improving the streptococcus iniae infection-resisting capacity of GIFT oreochromis niloticus during high-temperature stress. The method comprises the following steps: preparing basic ration and a compound Chinese medicinal herb formula, and feeding feed. Due to the adoption of the method, the immunity index of the GIFT oreochromis niloticus during high-temperature stress can be effectively increased, the liver damage is reduced, meanwhile, the streptococcus iniae infection-resisting capacity of the GIFT oreochromis niloticus can be improved, the morbidity is reduced, and the survival rate of the GIFT oreochromis niloticus infected with streptococcus iniae can be increased. Several Chinese medicinal herbs involved in the method are relatively available and relatively low in cost; a production technology is simple. The basic ration with the compound Chinese medicinal herbs has relatively good palatability, and after the basic ration is taken by the GIFT oreochromis niloticus, drug residues in bodies of the GIFT oreochromis niloticus are relatively few, and therefore, the GIFT oreochromis niloticus meets the requirements on green healthy aquatic products.

The invention discloses a ZIKV (zika virus) vaccine. The ZIKV vaccine contains or can express membrane protein prM / M, envelope protein E and non-structural protein NS1 of ZIKV. The invention also discloses a preparation method of the ZIKV vaccine. The ZIKV vaccine has higher immunogenicity and can more effectively prevent ZIKV infection.

The invention discloses a method for Agrobacterium-mediated genetic transformation of sugarcane growth points, and belongs to the technical field of sugarcane cultivation. The method comprises the following steps: selecting a genetically transformed sugarcane material, a strain and plasmid, preparing a callus induction medium M1, a differentiation medium M2, a rooting medium M3, a transformation medium MR and a co-medium M4, inducing and differentiating sugarcane callus, preparing an Agrobacterium engineering bacterial solution, using an Agrobacterium-mediated method to perform genetic transformation on the sugarcane growth points, and testing transgenic plants. The method shortens the time for sugarcane to obtain transformed plants, and improves the transformation efficiency. Through theAgrobacterium-mediated transformation technology, a pCAMBIA3301 plant expression vector is introduced into GT35 sterile seedlings, a total of 353 resistant plants are obtained after screening by a screening medium, the resistant plants are tested by PCR, 333 plants are positive, a positive rate reaches to 94.33%, and thereby the result indicates that the foreign gene is successfully introduced into sugarcane.

The invention discloses a method for earthing-cultivating silkworm insect grass by simulating an ecological environment. The method comprises the following steps: (1) selecting a strain host; (2) enlarging preparation solution of cultivated strain liquid; (3) preprocessing and postprocessing inoculation; (4) preparing an embedding substrate; and (5) management of earthing-cultivating: inoculatingthe strains optimized in the step (2) in a fluid nutrient medium added with 5-8 wt% of silkworm chrysalis meal, after enlarging the cultivation, inoculating in healthy silkworm larvae in the step (1)for infecting and rigidifying, and embedding infected and rigidified silkworm bodies to be developed in the step (3) in a transparent plastic box filled with the plant ash embedding substrate; after sporophore is grown to be matured, taking out the silkworm bodies from the plant ash embedding substrate, and cleaning up plant ash adhered to the periphery of the silkworm bodies, to obtain the matured silkworm insect grass. The method has the characteristics of low sundry fungus contamination rate, high infection rigidifying rate, high production rate, good stability of the obtained silkworm insect grass, higher yield, low cost, and full and regular appearance of the silkworm insect grass, is suitable for the large-scale production, and has the extensive development prospect.

The invention discloses an ecological planting method of lucid ganoderma, and relates to the technical field of lucid ganoderma planting. The method includes the first step of land selection, the second step of shed erection and soil preparation, the third step of strain selection, the fourth step of culture medium treatment, the fifth step of culture medium inoculation, the sixth step of lucid ganoderma culture, and the seventh step of lucid ganoderma harvesting. The planting method is ecological, scientific and high in stability, effectively increases the yield of lucid ganoderma and effectively improves the quality of lucid ganoderma.

The invention relates to an antiviral growth promoting premix feed, which comprises the following ingredients in mass percentage: 3-8 percent of astragalus extracts; 3-8 percent of ganoderma extracts; 1-3 percent of hypericum extracts; 5-10 percent of vitamin C; 1-3 percent of methionine; 0.5-1.5 percent of lysine; and the balance of auxiliary materials. The antiviral growth promoting premix feed can increase the weight of meat poultry, improve the meat quality, reduce leg diseases, improve the survival rate of piglets, promote fat pigs to grow and develop, reduce the ratio of feed to gain and shorten the marketing time.

The invention relates to a chewing gum for preventing and treating oral ulcer after chemotherapy. The chewing gum is composed of chewing gum ingredients and drugs used for preventing and treating oral ulcer. Each 100 g of the chewing gum for preventing and treating oral ulcer after chemotherapy comprises the following drugs used for preventing and treating oral ulcer: 5 to 10 mg of a recombinant human granulocyte colony stimulating factor, 5 to 10 mg of folic acid, 5 to 10 mg of metronidazole, 20 to 50 mg of lidocaine, 5 to 20 mg of cimetidine and 2.5 to 10 mg of vitamin B2, with the balance being the chewing gum ingredients. The chewing gum ingredients comprise, by weight, 25 to 30% of an edible gum base, 20 to 25% of maltose syrup, 35 to 45% of xylitol and 10 to 20% of sorbitol. The chewing gum provided by the invention can effectively prevent and treat oral ulcer resulting from different causes after chemotherapy, mitigate stimulation of the drugs on gastrointestinal tracts, reduce occurrence of adverse reactions and improve safety, has good taste and is convenient to take.

The invention discloses a II-set alphabaculovirus shuttle expression vector MbBacmid capable of expressing foreign protein in various insect cells and application thereof. The Bacmid contains whole genome sequences of mamestra brassicae nuclear polyhedrosis viruses, and contains genetic elements capable of being copied in escherichia coli. Foreign protein genes can be quickly inserted into the Bacmid provided by the invention through a donor plasmid pFast-Bac Mbph, and are efficiently expressed inside various commercial insect cells including Sf9, Tn368, high five and the like. The vector and a donor plasmid pFast-Bac Mbph+piel-cath can be subjected to homologous recombination to obtain a recombinant virus MbMNPV-ph+ piel-cath capable of increasing insect killing speed. The expression system provided by the invention is the only II-set alphabaculovirus shuttle expression vector system capable of efficiently expressing foreign protein in various commercial insect cells.

The invention belongs to the technical field of healthcare products, and concretely relates to an immunity enhancing healthcare food prepared through mixing wall-broken Mythic Fungus spore powder with a Mythic Fungus extract. The healthcare product has the advantages of easy production, low cost, good mouthfeel, easy absorption, enhancement of the pathogen infection resistance of a body, and improvement of the health level of the body, and is a good immunity enhancing healthcare food.

The invention discloses a chimeric simian / human immunodeficency virus strain and application thereof, relating to a virus modified with an introduced exogenetic gene material. The virus is a simian / human immunodeficency virus SHIV-XJN0363B8 carrying a China HIV-1 (Human Immunodeficiency Virus) B' / C recombinant subtype envelope protein gene segment, has the classification name as immunodeficency virus and the Latin name as Lentivirus, and is preserved with the collection number of CGMCC No. 4243 on Oct. 15th, 2010 in China General Microbiological Culture Collection Center with the address of No.3, yard 1, Beichen west road, Chaoyang District, Beijing. An SHIV-XJN0363B8 / China rhesus monkey AIDS (Acquired Immune Deficiency Syndrome) model built by using the virus strain SHIV-XJN0363B8 can beused for effectively simulating the virus replication characteristic at the early stage of HIV-1 infection, and play roles in researching the in-vivo pathogenic mechanism of HIV-1 and evaluating the effectiveness of biological agents, such as AIDS vaccines, AIDS antiviral medicaments and the like, in China.

The invention relates to a medicine composite containing erythromycin thiocyanate and a preparation method thereof. The composite comprises erythromycin thiocyanate, stabilizers, solubilizer and auxiliary materials. The preparation method includes the steps that the stabilizers, the solubilizer and erythromycin thiocyanate are evenly mixed, and the mixture is screened by a screen of 80-120 meshes in a smashed mode to obtain a semi-finished product; the auxiliary materials are screened by a screen of 80-100 meshes in a smashed mode to be thrown into a mixing tank; then the semi-finished product is added, even mixing is performed, and the medicine composite is obtained through sub-packaging. The approximate solubility of the medicine composite containing erythromycin thiocyanate in water is remarkably improved, stability is good, and through six months of acceleration tests, all detection items meet quality standards. The water solution of the medicine composite is not likely to be affected by temperature, water quality hardness and other objective factors, the content of effective components in drinking water can be kept unchanged for 24 hours, exertion of the medicine effect is guaranteed, and the effect of resisting gram-positive bacteria and mycoplasma infection is remarkably improved for livestock.

The invention discloses a freshness keeping device for fruits and vegetables in supermarkets, and belongs to the technical field of freshness keeping devices for fruits and vegetables. The freshness keeping device comprises a temperature detection unit, a humidity detection unit, a carbon dioxide concentration detection unit, a control unit, a temperature control unit, a humidity control unit, an ozone generation unit and a high-pressure electrostatic generation unit, wherein the high-pressure electrostatic generation unit comprises a direct-current high-voltage power supply, a positive-negative high-voltage filter circuit, a positive-negative ionic discharge electrode, a frequency producing module and a frequency selection module. According to the device disclosed by the invention, a high-voltage electrostatic field is adopted, so that the respiratory metabolism activity of the vegetables and the fruits is restrained, moisture content in the vegetables and the fruits is effectively maintained, and a freshness keeping effect is achieved.

The invention relates to a composite porcine alpha-IFN gene and a recombinant vector thereof, and belongs to the technical field of genetic engineering biological products. The newly designed gene sequences of composite porcine alpha-IFN are recombined to pPICZ alpha-A vectors, and the vectors are electronically transformed into yeast competent cells; and the positive identification, and the screening and the induced expression of high copy yeast strains are carried out. A pichia pastoris expression system is used for expressing foreign genes so as to be favorable for the commercial production of porcine IFN. Compared with the natural IFN which is expressed by yeast, the expression level of the protein which is expressed by genome increases by 3.17 times. The fluorescent quantitative PCR result shows that the level of PRVmRNA after PK-15 cells treated by 10-6mg / ml of the composite porcine alpha-IFN are re-infected with PRV is obviously lower than the level of PRVmRNA after the PK-15 cells which are not treated by the composite porcine alpha-IFN are infected with the PRV, and the level of PRVmRNA decreases by 37 times.

The invention provides application of cyclic dipeptide in preparation of drugs for treating skin injury. The cyclic dipeptide is cyclo-L-prolyl-L-dipeptide, and is particularly used for burns and scalds. The dipeptide has the features of small molecular weight, simple structure and the like. The cyclic dipeptide has a remarkable effect on treatment of burns and scalds, can quickly permeate injured skin tissues and enter cells in a treatment process, repairs injured cells and activates self-repairing capability, wound healing speed is accelerated, recurrence rate is low after healing, scars are shallow, and potential side effects after treatment by traditional Chinese medicines and western drugs are avoided. Moreover, the cyclic dipeptide also has effects of inhibiting inflammation and stopping pain, and improving immunity.

The invention discloses a recombinant mutant adeno-associated virus capable of efficiently infecting primary microglial cells and a related biological material of the recombinant mutant adeno-associated virus. The recombinant adeno-associated virus is an adeno-associated virus for expressing AAV-6X type capsid protein, and the AAV-6X type capsid protein is protein with an amino acid sequence as shown in a sequence 4 in a sequence table. According to the recombinant mutant adeno-associated virus, wild AAV-6 type capsid protein is subjected to the following mutation to obtain the mutated AAV-6 type capsid protein: seven amino acid residues are inserted between the 588 site and the 589 site of the amino acid sequence of the AAV-6 type capsid protein. The efficiency of in-vitro microglial cell infection of the recombinant adeno-associated virus rAAV-6X (expressing the AAV-6X type capsid protein) is 8 times that of the recombinant adeno-associated virus rAAV-6 (expressing the wild type AAV-6 type capsid protein). The recombinant adeno-associated virus provided by the invention is an rAAV vector for efficiently transducing the microglial cells.

The invention relates to the field of proteins, and particularly relates to a bioactive peptide AGYDVEKNNSRIKLGLK and a preparation method and application thereof. The amino acid sequence of the bioactive peptide AGYDVEKNNSRIKLGLK is Ala-Gly-Tyr-Asp-Val-Glu-Lys-Asn-Asn-Ser-Arg-Ile-Lys-Leu-Gly-Leu-Lys. In-vitro immune function regulation experiments verify that the bioactive peptide AGYDVEKNNSRIKLGLK has a good immune regulation function. The bioactive peptide AGYDVEKNNSRIKLGLK can promote macrophage activation and release cell factors, has the capacity of promoting increase of macrophage nitric oxide induction amount under a certain concentration condition, improves the capacity of resisting external pathogen infection of the body, reduces the morbidity of the body, improves the life quality and has very important significance in developing food, health care products and medicine with the immune regulation function.