Pseudomonas aeruginosa, klebsiella and pasteurella triple-inactivated vaccine for mink
A technology of mink Pseudomonas aeruginosa and Klebsiella, which is applied in the field of economic animal vaccine production, can solve the problems of increasing the infection rate, and achieve the effects of improving immune protection rate, preventing mixed infection, and preventing mink bacterial diseases
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[0022] Preparation of triple inactivated vaccine against Pseudomonas aeruginosa, Klebsiella and Pasteurella multocida
[0023] 1. Seed preparation for production
[0024] (1) Primary seed preparation
[0025] Inoculate the basic strain of Pasteurella multocida on a Martin agar plate containing 0.1% lysed blood and 4% healthy animal serum, culture it at 36-37°C for 24 hours, then select more than 5 typical colonies and inoculate it on blood agar Several branches on the slant, cultured at 36-37°C for 24 hours, as first-class seeds, stored at 2-8°C, and the use period should not exceed 14 days. Passage should not exceed 5 generations.
[0026] Inoculate the basic strains of Pseudomonas aeruginosa and Klebsiella on the agar plate of ordinary broth medium, culture at 36-37°C for 24 hours, then select more than 5 typical colonies, and inoculate several branches of ordinary agar slant, 36-37°C Cultivate at 37°C for 24 hours, store it at 2-8°C as first-class seeds, and use it fo...
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