Pseudomonas aeruginosa, klebsiella and pasteurella triple-inactivated vaccine for mink

A technology of mink Pseudomonas aeruginosa and Klebsiella, which is applied in the field of economic animal vaccine production, can solve the problems of increasing the infection rate, and achieve the effects of improving immune protection rate, preventing mixed infection, and preventing mink bacterial diseases

Active Publication Date: 2015-07-01
JILIN HEYUAN BIOENG LIMITED
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Therefore, to a certain extent, monovalent vaccines cannot achieve good results in preventing multiple diseases. At the same time, the use of multiple monovalent vaccines often has multiple contraindications, which also increases the chance of infection

Method used

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  • Pseudomonas aeruginosa, klebsiella and pasteurella triple-inactivated vaccine for mink
  • Pseudomonas aeruginosa, klebsiella and pasteurella triple-inactivated vaccine for mink

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Preparation of triple inactivated vaccine against Pseudomonas aeruginosa, Klebsiella and Pasteurella multocida

[0023] 1. Seed preparation for production

[0024] (1) Primary seed preparation

[0025] Inoculate the basic strain of Pasteurella multocida on a Martin agar plate containing 0.1% lysed blood and 4% healthy animal serum, culture it at 36-37°C for 24 hours, then select more than 5 typical colonies and inoculate it on blood agar Several branches on the slant, cultured at 36-37°C for 24 hours, as first-class seeds, stored at 2-8°C, and the use period should not exceed 14 days. Passage should not exceed 5 generations.

[0026] Inoculate the basic strains of Pseudomonas aeruginosa and Klebsiella on the agar plate of ordinary broth medium, culture at 36-37°C for 24 hours, then select more than 5 typical colonies, and inoculate several branches of ordinary agar slant, 36-37°C Cultivate at 37°C for 24 hours, store it at 2-8°C as first-class seeds, and use it fo...

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Abstract

The invention discloses a pseudomonas aeruginosa, klebsiella and pasteurella triple-inactivated vaccine for a mink. The vaccine is composed of inactivated pseudomonas aeruginosa, klebsiella and pasteurella, and is capable of simultaneously preventing a pseudomonas aeruginosa disease, a klebsiella disease and a pasteurella disease, and mixed infection with pseudomonas aeruginosa, klebsiella disease and pasteurella disease. The vaccine is small in side effect, has synergistic effect, has relatively good immunogenicity and safety, and can reach multiple prevention effects; the immune procedure for vaccination is reduced; and the protection efficiency is improved.

Description

technical field [0001] The invention discloses a triple inactivated vaccine of Pseudomonas aeruginosa, Klebsiella and Pasteurella multocida and a preparation method thereof. The invention can be used for preventing Pseudomonas aeruginosa, Klebsiella, Pasteurella multocida and the mixed infection of the three in minks, and relates to the technical field of economic animal vaccine production. Background technique [0002] Mink Pseudomonas aeruginosa, the pathogen is Pseudomonas aeruginosa, because it can produce green pus after invading the body, so it is customarily called Pseudomonas aeruginosa. Pseudomonas aeruginosa is an opportunistic pathogen that exists in the body of normal healthy animals. When the body is stressed or the immune function is weakened, it has the opportunity to infect the body. Pseudomonas aeruginosa is a Gram-negative bacillus or coccus, which does not require high nutrition and can grow in ordinary medium. The medium is stained blue-green due to the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/102A61P31/04A61K39/108A61K39/104
Inventor 张中洋张国军扈荣良吴金张守峰刘晔王宏宇
Owner JILIN HEYUAN BIOENG LIMITED
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